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    • 3. 发明申请
    • FOCUSED ACOUSTIC EJECTION CELL SORTING SYSTEM AND METHOD
    • 聚焦呼吸细胞分选系统及方法
    • WO02054044A2
    • 2002-07-11
    • PCT/US0150753
    • 2001-12-28
    • PICOLITER INC
    • MUTZ MITCHELL WELLSON RICHARD NLEE DAVID SOONG-HUA
    • G01N33/48B41J2/14C12M1/26C12M1/34C12M3/00C12N5/00C12Q1/02G01N1/28G01N15/10G01N15/14G01N30/02G01N37/00G01N15/00
    • B41J2/14008C12M47/04G01N15/1056G01N15/1456G01N29/028G01N30/02G01N35/1074G01N2015/1081G01N2015/1415G01N2015/142G01N2015/1486G01N2015/149G01N2035/1039B01D2015/389
    • A method is provided for acoustically ejecting from a channel or other container a plurality of fluid droplets, each of which contains one or more particles or other localized volumes. The localized volumes, which can be living cells, are ejected towards sites on a substrate surface, a container, or a channel. An integrated cell sorting and arraying system is also provided that is capable of sorting based upon cellular properties by the selective ejection of cells from a carrier fluid. The cells can be ejected with adjustable velocity and trajectory. The ejected cells can be directed to form an array, wherein each site of the array can contain a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly, and economically than by other cell array approaches. This method permits efficient, continuous, and simultaneous sorting of cells based upon the quantitative or semiquantitative measurement of cellular properties, and also permits non-binary or severally-branched decision-making. An integrated system, which includes a processor, and methods are also provided for the detection, selection, and ejection of selected particles or circumscribed volumes, such as live cells, from a continuous stream of fluid-suspended particles or other circumscribed volumes flowing in channels.
    • 提供了一种用于从通道或其它容器声学喷射多个流体液滴的液滴,每个流体液滴包含一个或多个颗粒或其它局部容积。 可以是活细胞的局部体积被排出到基底表面,容器或通道上的位置。 还提供了一种综合的细胞分选和排列系统,其能够通过从载体液体中选择性地排出细胞而基于细胞特性进行分选。 可以以可调节的速度和轨迹弹出细胞。 排出的细胞可以被引导以形成阵列,其中阵列的每个位点可以包含单个细胞。 另外提供了一种形成单个活细胞阵列的方法,其比单体阵列方法更有效,快速,灵活和经济地形成。 该方法允许基于细胞性质的定量或半定量测量来对细胞进行有效,连续和同时的分选,并且还允许非二元或分支分支的决策。 还提供了一种包括处理器和方法的集成系统,用于从流体悬浮颗粒的连续流或在通道中流动的其它外接体积检测,选择和排出所选择的颗粒或外切体积(例如活细胞) 。
    • 6. 发明申请
    • DIFFERENTIAL ULTRASONIC WAVEGUIDE CURE MONITORING PROBE
    • 差分超声波波形监测探测器
    • WO2009152143A1
    • 2009-12-17
    • PCT/US2009/046729
    • 2009-06-09
    • MATERIALS AND SENSORS TECHNOLOGIES, INC.DJORDJEVIC, Borislav, B.ROUCH, Lawrence, L.
    • DJORDJEVIC, Borislav, B.ROUCH, Lawrence, L.
    • G01N11/00
    • G01N29/11G01N29/028G01N29/09G01N29/2462G01N29/4427G01N2203/0092G01N2291/0251
    • The present invention is seen to provide a new methodology, testing system designs and concept to enable in situ real time monitoring of the cure process. Apparatus, system, and method for the non-destructive, in situ monitoring of the time dependent curing of advanced materials using one or more differential ultrasonic waveguide cure monitoring probes. A differential ultrasonic waveguide cure monitoring probe in direct contact with the material to be cured and providing in situ monitoring of the cure process to enable assessment of the degree of cure or cure level in a non-cure related signal variances (e.g., temperature) independent calibrated response manner. A differential ultrasonic waveguide cure monitoring probe including a transducer coupled to a waveguide and incorporating correction and calibration methodology to accurately and reproducibly monitor the cure process and enable assessment of cure level via ultrasonic reflection measurements. The amplitude of the corrected interface response signal reflected from the probe-resin interface indicating changes in the modulus of the material during the cure.
    • 本发明可以提供一种新的方法,测试系统的设计和概念,以实现对固化过程的实时监测。 用于使用一个或多个差分超声波波导固化监测探针对先进材料进行时间依赖固化的非破坏性,现场监测的装置,系统和方法。 差示超声波波导固化监测探头与要固化的材料直接接触,并提供固化过程的现场监测,以便能够在非固化相关信号方差(例如温度)独立性下评估固化程度或固化水平 校准响应方式。 差示超声波波导固化监测探头,其包括耦合到波导的传感器并且包含校正和校准方法以精确和可重复地监测固化过程,并且能够通过超声波反射测量来评估固化水平。 校正的界面响应信号的幅度从探针 - 树脂界面反射,指示在固化期间材料的模量的变化。
    • 9. 发明申请
    • REFLECTION-ONLY SENSOR FOR FLUID ACOUSTIC IMPEDANCE, SOUND SPEED, AND DENSITY
    • 用于流体声学阻抗,声速和密度的反射式传感器
    • WO2015112373A1
    • 2015-07-30
    • PCT/US2015/011141
    • 2015-01-13
    • BAKER HUGHES INCORPORATED
    • DIFOGGIO, Rocco
    • E21B47/00E21B49/08G01V1/40G01V1/48
    • G01N29/028E21B47/101G01N29/024G01N2291/02818G01N2291/044
    • Methods, systems, and devices for estimating a parameter of interest of a downhole fluid. Methods may include using at least two solid acoustic transmission media, including a first media and a second media having different acoustic impedances and each having a corresponding face immersed in the downhole fluid. Methods may include using a reflection of an acoustic pulse from a solid-liquid interface at the corresponding face of the first media and the second media to estimate the parameter of interest. The sound speed and the acoustic impedance of the first media and the sound speed and the acoustic impedance of the second media may each be different than a sound speed and an acoustic impedance of the fluid and each other. The parameter of interest may be estimated independently of a time of flight in the downhole fluid of any acoustic pulses.
    • 用于估计井下流体感兴趣的参数的方法,系统和装置。 方法可以包括使用至少两个固体声学传播介质,包括具有不同声阻抗的第一介质和第二介质,并且每个具有浸入井下流体中的相应面。 方法可以包括使用来自第一介质和第二介质的相应面处的固 - 液界面的声脉冲的反射来估计感兴趣的参数。 第一介质的声速和声阻抗以及第二介质的声速和声阻抗可以各自不同于流体的声速和声阻抗。 感兴趣的参数可以独立于任何声学脉冲的井下流体中的飞行时间来估计。