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1. 发明申请

WO00015829A1 FERMENTATION MEDIUM AND METHOD FOR PRODUCING alpha , omega -ALKANEDICARBOXYLIC ACIDS 审中-公开
标题翻译：发酵培养基和生产α，ω-烷基羧酸的方法
公开(公告)号：WO00015829A1
公开(公告)日：2000-03-23
申请号：PCT/US1999/011751
申请日：1999-05-27
IPC分类号： C12N1/16 , C12P7/44 , C12R1/74
CPC分类号： C12R1/74 , C12N1/16 , C12P7/44
摘要： This invention describes a low cost biofermentation medium, and an economical method using the medium, for the manufacture of alpha , omega -alkanedicarboxylic acids. The invention provides a biofermentation medium and a method of bioproduction for these important diacids which makes their large scale commercial production economically feasible using a biocatalyst. This method of production obviates the need for chemical synthesis using expensive starting materials from fossil fuels, and does not generate a costly hazardous waste stream.
摘要翻译：本发明描述了一种低成本生物发酵介质，以及使用该介质的经济方法，用于制造α，ω-烷二羧酸。本发明提供了一种生物发酵培养基和这些重要二酸的生物生产方法，其使得其大规模商业生产在生物催化剂上经济上可行。这种生产方法避免了使用来自化石燃料的昂贵原料进行化学合成的需要，并且不会产生昂贵的危险废物流。

2. 发明申请

WO2014121428A1 一种产长链二元酸的热带假丝酵母菌的诱变方法审中-公开
公开(公告)号：WO2014121428A1
公开(公告)日：2014-08-14
申请号：PCT/CN2013/001409
申请日：2013-11-18
申请人：徐杰
发明人：徐杰 , 王强
IPC分类号： C12N15/01 , C12N13/00 , C12N1/16 , C12R1/74
CPC分类号： C12P7/44 , C12N1/16 , C12N15/01 , C12P7/6409 , C12R1/74
摘要：本发明涉及一种产长链二元酸的热带假丝酵母菌的诱变方法，该方法是先制备培养基，将热带假丝酵母菌接种于培养基上，采用放射源钴60γ-射线进行辐照诱变，并用诱变筛选培养基系统筛选获得高产长链二元酸的热带假丝酵母菌的突变菌株，从而提高长链二元酸的产酸量和正构烷烃的转化率。

3. 发明申请

WO2017037745A1 AN INTEGRATED PROCESS FOR PRODUCTION OF CARBOHYDRATASES, ETHANOL, AND XYLITOL USING AN ISOLATED CANDIDA STRAIN 审中-公开
标题翻译：使用分离的甘地酸菌株生产碳水化合物，乙醇和XYLITOL的综合方法
公开(公告)号：WO2017037745A1
公开(公告)日：2017-03-09
申请号：PCT/IN2016/050292
申请日：2016-09-02
申请人： HINDUSTAN PETROLEUM CORPORATION LIMITED
发明人： VELANKAR, Harshad Ravindra , MATTAM, Anu Jose , KUILA, Arindam , PEDDY, Venkata Chalapathi Rao , NETTEM, Venkateswarlu Choudary
IPC分类号： C12R1/74 , C12N9/14 , C12P7/00
CPC分类号： C12R1/74 , C12N1/22 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12P7/065 , C12P7/10 , Y02E50/16 , Y02E50/17 , Y02P20/125
摘要： The present disclosure discloses a novel strain of Candida tropicalis (MTCC 25057) which can grown in a wide range of process conditions (temperature, pH), and in the presence of high concentrations of lignocellulosic inhibitors. The said strain has the capacity for simultaneous production of cellulases and xylanses from fermentation of sugars from lignocellulosic mass for production of ethanol, and xylitol.
摘要翻译：本公开公开了一种新型热带假丝酵母菌株（MTCC 25057），其可以在广泛的工艺条件（温度，pH）下以及在高浓度的木质纤维素抑制剂存在下生长。所述菌株具有从用于生产乙醇和木糖醇的木质纤维素块发酵糖同时生产纤维素酶和木聚糖的能力。

4. 发明申请

WO00015828A1 PROCESS FOR MAKING POLYCARBOXYLIC ACIDS 审中-公开
标题翻译：制备聚碳酸的方法
公开(公告)号：WO00015828A1
公开(公告)日：2000-03-23
申请号：PCT/US1998/018494
申请日：1998-09-17
IPC分类号： C12P7/44 , C12P7/46 , C12N1/16
CPC分类号： C12P7/44 , C07C51/34 , C12P7/6409 , C12R1/74 , Y10S435/924 , C07C55/18
摘要： Aliphatic polycarboxylic acids are made by a process comprising the steps of: (1) fermenting a beta-oxidation blocked C. tropicalis cell wherein both copies of the chromosomal POX5 gene and the chromosomal POX4A and POX4B genes are disrupted in a culture medium comprised of a nitrogen source, an organic substrate and a cosubstrate wherein the substrate is an unsaturated aliphatic compound having at least one internal carbon-carbon double bond and at least one terminal methyl group, a terminal carboxyl group and/or a terminal functional group which is oxidizable to a carboxyl group by biooxidation; (2) reacting the product of step (1) with an oxidizing agent to produce one or more polycarboxylic acids.
摘要翻译：脂肪族多元羧酸通过包括以下步骤的方法制备：（1）发酵β-氧化封闭的C。热带异常细胞，其中染色体POX5基因和染色体POX4A和POX4B基因的两个拷贝在包含氮源，有机底物和辅助底物的培养基中被破坏，其中所述底物是至少具有不饱和脂族化合物一个内部碳 - 碳双键和至少一个末端甲基，通过生物氧化可氧化成羧基的末端羧基和/或末端官能团; （2）使步骤（1）的产物与氧化剂反应以产生一种或多种多元羧酸。

5. 发明申请

WO2011112627A1 METHODS, BIOLOGICAL OILS, BIOFUELS, UNITS, AND ORGANISMS RELATED TO USE IN COMPRESSION ENGINES 审中-公开
标题翻译：方法，生物油，生物柴油，单位和与压缩发动机相关的有机体
公开(公告)号：WO2011112627A1
公开(公告)日：2011-09-15
申请号：PCT/US2011/027610
申请日：2011-03-08
申请人： BP BIOFUELS UK LIMITED , PFEIFER, Joseph, W. , BEHRENS, Paul, W. , JOHNSON, Michael, A. , HANSEN, Jon, M.
发明人： PFEIFER, Joseph, W. , BEHRENS, Paul, W. , JOHNSON, Michael, A. , HANSEN, Jon, M.
IPC分类号： C12P7/64 , C12N1/14 , C12N1/16 , C12R1/645 , C12R1/74
CPC分类号： C12R1/645 , C12P7/6463 , C12P7/649 , C12R1/74 , Y02E50/13
摘要： Methods, biological oils, biofuels, units, and/or organisms directed to use in compression engines. A method of producing biological oils includes producing an organism and having the organism consume a feedstock. The organism includes a lipid containing fatty acids. The organism meets or exceeds at least two metrics. The metrics include: A) a eel! density of at least about 115 grams per liter; B) a fatty acid content of at least about 49 percent on a dry mass basis; C) a fatty acid productivity of at least about 15 grams per liter per day; D) a fatty acid yield of at least about 0.175 grams of fatty acids produced per grams of the feedstock consumed; E) a 24 hour peak fatty acid productivity of at least about 30 grams per liter per day: F) an extraction efficiency on a percent of total fatty acid content basis of at least about 50 percent; and/or G) yield of fatty acids on oxygen of more than about 0.4 as grams of fatty acids produced per gram of oxygen consumed basis.
摘要翻译：方法，生物油，生物燃料，单位和/或指导用于压缩发动机的生物。生产生物油的方法包括生产生物体并使生物体消耗原料。生物体包含含脂质的脂肪酸。生物达到或超过至少两个指标。指标包括：A）鳗鱼！至少约115克/升的密度; B）以干质量计至少约49％的脂肪酸含量; C）每天至少约15克/升的脂肪酸生产率; D）每克消耗的原料产生的至少约0.175克脂肪酸的脂肪酸产率; E）每天至少约30克的24小时峰值脂肪酸生产率：F）总脂肪酸含量百分比的提取效率至少为约50％; 和/或G）在氧上的脂肪酸的产量大于约0.4，作为每克氧消耗基础产生的克分子量。

6. 发明申请

WO2004029263A3 PROCESS FOR ACTINOL PRODUCTION FROM KETOISOPHORONE 审中-公开
标题翻译：从酮基异硫氰酸酯生产乙酰乙酸的方法
公开(公告)号：WO2004029263A3
公开(公告)日：2004-05-27
申请号：PCT/EP0310295
申请日：2003-09-16
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , SETOGUCHI YUTAKA , SHIMIZU SAKAYU , TABATA KAZUYUKI
发明人： HOSHINO TATSUO , SETOGUCHI YUTAKA
IPC分类号： C12P7/26
CPC分类号： C12R1/645 , C12P7/26 , C12R1/15 , C12R1/74 , C12R1/865
摘要： Disclosed is a process for producing actinol from ketoisophorone which comprises contacting ketoisophorone with a recombinant microorganism or cell-free extract thereof in a reaction mixture, wherein said recombinant microorganism is obtainable by transforming a host microorganism, e.g. selected from the group consisting of microorganisms of the genera Saccharomyces, Zygosaccharomyces, and Candida, such as commercially available baker's yeast, Saccharomyces cerevisiae ATCC7754, Saccharomyces rouxii (Zygosaccharomyces rouxii) HUT7191 (IFO 0494), Saccharomyces delbrueckii HUT7116 (Saccharomyces unisporus IFO 0298), Saccharomyces delbrueckii (Torulaspora delbrueckii) HUT7102, Saccharomyces willianus HUT7106, Zygosaccharomyces bailii ATCC11486, Candida tropicalis IFO 1403, and a mutant thereof, which is capable of reducing ketoisophorone to levodione with a levodione reductase gene, e.g. a levodione reductase gene derived from a microorganism belonging to the genus Corynebacterium, such as C. aquaticum AKU611 (FERM BP-6448) or a mutant thereof, and isolating the produced actinol from the reaction mixture.
摘要翻译：公开了一种由酮基异佛尔酮制备放线醇的方法，该方法包括将酮基异佛尔酮与重组微生物或其无细胞提取物在反应混合物中接触，其中所述重组微生物可通过转化宿主微生物例如甲醇，（Saccharomyces cerevisiae）ATCC7754，鲁氏酵母（Saccharomyces rouxii）（Zygosaccharomyces rouxii），HUT7191（IFO 0494），德氏乳杆菌HUT7116（Saccharomyces unisporus IFO 0298），酵母属假丝酵母属德氏乳杆菌（Torulaspora delbrueckii）HUT7102，Saccharomyces willianus HUT7106，Zygosaccharomyces bailii ATCC11486，热带假丝酵母IFO 1403及其突变体，其能够将酮异佛尔酮用左旋二酮还原酶基因还原成左旋二酮，例如来自棒状杆菌属的微生物如水生假丝酵母AKU611（FERM BP-6448）或其突变体的左旋二酮还原酶基因或其突变体，并从反应混合物中分离所产生的放线醇。

7. 发明申请

WO2003100013A2 NON-REVERTIBLE β-OXIDATION BLOCKED CANDIDA TROPICALIS 审中-公开
标题翻译：不可逆的β-氧化阻滞的甘地酸热带
公开(公告)号：WO2003100013A2
公开(公告)日：2003-12-04
申请号：PCT/US2003/016453
申请日：2003-05-22
申请人： COGNIS CORPORATION
发明人： CRAFT, David, L.
IPC分类号： C12N
CPC分类号： C12R1/74 , C12P7/44
摘要： Genetically modified strains of C. tropicalis, which will not revert to wild-type activity at the POX 4 and/or POX 5 locus, are disclosed. The strains are β -oxidation blocked and have been transformed through homologous recombination with a construct which deletes a portion of the POX 4 and/or POX 5 gene. The modified strains may be used to increase yields of dicarboxylic acids produced in host cells of the strains. Methods for blocking the β -oxidation pathway in a C. tropicalis host cell are also provided.
摘要翻译：披露了热带假丝酵母的转基因菌株，其不会在POX 4和/或POX 5基因座处恢复野生型活性。菌株被β-氧化阻断，并通过与删除一部分POX 4和/或POX 5基因的构建体进行同源重组而转化。修饰的菌株可用于增加菌株宿主细胞中产生的二羧酸的产量。还提供了用于阻断热带假丝酵母宿主细胞中的β-氧化途径的方法。

8. 发明申请

WO1991006660A1 SITE-SPECIFIC MODIFICATION OF THE CANDIDA TROPICALIS GENOME 审中-公开
标题翻译： CANDIDA TROPICALIS基因组的特异性修饰
公开(公告)号：WO1991006660A1
公开(公告)日：1991-05-16
申请号：PCT/US1990006427
申请日：1990-11-06
申请人： HENKEL RESEARCH CORPORATION
发明人： HENKEL RESEARCH CORPORATION , PICATAGGIO, Stephen , DEANDA, Kristine , EIRICH, L., Dudley
IPC分类号： C12P07/44
CPC分类号： C12R1/74 , C12N15/00 , C12N15/01 , C12N15/815 , C12N15/90 , C12P7/44 , Y10S435/924
摘要： The POX genes of C. tropicalis are disrupted resulting in the complete blockage of the beta-oxidation pathway in the strain. Fermentation of C. tropicalis cells having disrupted genes on alkane, fatty acid and fatty acid ester substrates produces substantially pure dicarboxylic acids in substantially quantitative yield.

9. 发明申请

WO2011112627A8 METHODS, BIOLOGICAL OILS, BIOFUELS, UNITS, AND ORGANISMS RELATED TO USE IN COMPRESSION ENGINES 审中-公开
标题翻译：方法，生物油，生物燃料，单位和与压缩机使用有关的机构
公开(公告)号：WO2011112627A8
公开(公告)日：2012-04-26
申请号：PCT/US2011027610
申请日：2011-03-08
申请人： BP BIOFUELS UK LTD , PFEIFER JOSEPH W III , BEHRENS PAUL W , JOHNSON MICHAEL A , HANSEN JON M , APT KIRK E , BARCLAY WILLIAM R , MARTIN DAVID CHRISTIAN , BORDEN JACOB
发明人： PFEIFER JOSEPH W III , BEHRENS PAUL W , JOHNSON MICHAEL A , HANSEN JON M , APT KIRK E , BARCLAY WILLIAM R , MARTIN DAVID CHRISTIAN , BORDEN JACOB
IPC分类号： C12P7/64 , C12N1/14 , C12N1/16 , C12R1/645 , C12R1/74
CPC分类号： C12R1/645 , C12P7/6463 , C12P7/649 , C12R1/74 , Y02E50/13
摘要： Methods, biological oils, biofuels, units, and/or organisms directed to use in compression engines. A method of producing biological oils includes producing an organism and having the organism consume a feedstock. The organism includes a lipid containing fatty acids. The organism meets or exceeds at least two metrics. The metrics include: A) a eel! density of at least about 115 grams per liter; B) a fatty acid content of at least about 49 percent on a dry mass basis; C) a fatty acid productivity of at least about 15 grams per liter per day; D) a fatty acid yield of at least about 0.175 grams of fatty acids produced per grams of the feedstock consumed; E) a 24 hour peak fatty acid productivity of at least about 30 grams per liter per day: F) an extraction efficiency on a percent of total fatty acid content basis of at least about 50 percent; and/or G) yield of fatty acids on oxygen of more than about 0.4 as grams of fatty acids produced per gram of oxygen consumed basis.
摘要翻译：方法，生物油，生物燃料，单位和/或指导用于压缩发动机的生物。生产生物油的方法包括生产有机体并使生物体消耗原料。生物体包含含脂质的脂肪酸。生物达到或超过至少两个指标。指标包括：A）鳗鱼！至少约115克/升的密度; B）以干质量计至少约49％的脂肪酸含量; C）每天至少约15克/升的脂肪酸生产率; D）每克消耗的原料产生的至少约0.175克脂肪酸的脂肪酸产率; E）每天至少约30克/升的24小时峰值脂肪酸生产率：F）至少约50％的总脂肪酸含量百分比的提取效率; 和/或G）在氧上的脂肪酸的产量大于约0.4，作为克消耗的每克消耗的基础上生产的脂肪酸的克数。

10. 发明申请

WO2005010171A1 NOVEL CANDIDA TROPICALIS CJ-FID(KCTC 10457BP) AND MANUFACTURING METHOD OF XYLITOL THEREBY 审中-公开
标题翻译：新西兰花生酱CJ-FID（KCTC 10457BP）和XYLITOL THEREBY的制造方法
公开(公告)号：WO2005010171A1
公开(公告)日：2005-02-03
申请号：PCT/KR2004/001172
申请日：2004-05-18
申请人： CJ CORP. , KIM, Jung-hoon , SOHN, Young-rok , LEE, Woon-hwa , PARK, Seung-won , PARK, Kang-june , LEE, Ki-chang , LIM, Jae-kag
发明人： KIM, Jung-hoon , SOHN, Young-rok , LEE, Woon-hwa , PARK, Seung-won , PARK, Kang-june , LEE, Ki-chang , LIM, Jae-kag
IPC分类号： C12N1/16
CPC分类号： C12R1/74 , C12N11/10 , C12P7/18
摘要： Provided are a novel Candida tropicalis CJ-FID (KCTC 10457BP) derived from honey and a method for producing xylitol using the same. The method includes culturing the Candida tropicalis CJ-FID itself or an alginate-immobilized Candida tropicalis CJ-FID in a fermentation medium containing high concentration xylose and sucrose to obtain xylitol-containing culture, followed by purification with an activated charcoal column and anion column, to finally obtain a powdery xylitol. Therefore, high purity xylitol can be produced with high yield and productivity.
摘要翻译：本发明提供了一种来源于蜂蜜的新颖的热带假丝酵母CJ-FID（KCTC 10457BP）和使用其产生木糖醇的方法。该方法包括在含有高浓度木糖和蔗糖的发酵培养基中培养热带假丝酵母CJ-FID本身或藻酸盐固定的热带假丝酵母CJ-FID，得到含木糖醇的培养物，然后用活性炭柱和阴离子柱纯化，最终得到粉状木糖醇。因此，可以高产率和高生产率生产高纯度木糖醇。

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