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1. 发明申请

WO2004029263A3 PROCESS FOR ACTINOL PRODUCTION FROM KETOISOPHORONE 审中-公开
标题翻译：从酮基异硫氰酸酯生产乙酰乙酸的方法
公开(公告)号：WO2004029263A3
公开(公告)日：2004-05-27
申请号：PCT/EP0310295
申请日：2003-09-16
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , SETOGUCHI YUTAKA , SHIMIZU SAKAYU , TABATA KAZUYUKI
发明人： HOSHINO TATSUO , SETOGUCHI YUTAKA
IPC分类号： C12P7/26
CPC分类号： C12R1/645 , C12P7/26 , C12R1/15 , C12R1/74 , C12R1/865
摘要： Disclosed is a process for producing actinol from ketoisophorone which comprises contacting ketoisophorone with a recombinant microorganism or cell-free extract thereof in a reaction mixture, wherein said recombinant microorganism is obtainable by transforming a host microorganism, e.g. selected from the group consisting of microorganisms of the genera Saccharomyces, Zygosaccharomyces, and Candida, such as commercially available baker's yeast, Saccharomyces cerevisiae ATCC7754, Saccharomyces rouxii (Zygosaccharomyces rouxii) HUT7191 (IFO 0494), Saccharomyces delbrueckii HUT7116 (Saccharomyces unisporus IFO 0298), Saccharomyces delbrueckii (Torulaspora delbrueckii) HUT7102, Saccharomyces willianus HUT7106, Zygosaccharomyces bailii ATCC11486, Candida tropicalis IFO 1403, and a mutant thereof, which is capable of reducing ketoisophorone to levodione with a levodione reductase gene, e.g. a levodione reductase gene derived from a microorganism belonging to the genus Corynebacterium, such as C. aquaticum AKU611 (FERM BP-6448) or a mutant thereof, and isolating the produced actinol from the reaction mixture.
摘要翻译：公开了一种由酮基异佛尔酮制备放线醇的方法，该方法包括将酮基异佛尔酮与重组微生物或其无细胞提取物在反应混合物中接触，其中所述重组微生物可通过转化宿主微生物例如甲醇，（Saccharomyces cerevisiae）ATCC7754，鲁氏酵母（Saccharomyces rouxii）（Zygosaccharomyces rouxii），HUT7191（IFO 0494），德氏乳杆菌HUT7116（Saccharomyces unisporus IFO 0298），酵母属假丝酵母属德氏乳杆菌（Torulaspora delbrueckii）HUT7102，Saccharomyces willianus HUT7106，Zygosaccharomyces bailii ATCC11486，热带假丝酵母IFO 1403及其突变体，其能够将酮异佛尔酮用左旋二酮还原酶基因还原成左旋二酮，例如来自棒状杆菌属的微生物如水生假丝酵母AKU611（FERM BP-6448）或其突变体的左旋二酮还原酶基因或其突变体，并从反应混合物中分离所产生的放线醇。

2. 发明申请

WO2004029232A3 ACC GENE 审中-公开
标题翻译： ACC基因
公开(公告)号：WO2004029232A3
公开(公告)日：2004-10-14
申请号：PCT/EP0310683
申请日：2003-09-25
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , OJIMA KAZUYUKI , SETOGUCHI YUTAKA
发明人： HOSHINO TATSUO , OJIMA KAZUYUKI , SETOGUCHI YUTAKA
IPC分类号： C12N9/00 , C12N15/52 , C12P23/00
CPC分类号： C12N9/93 , C12P23/00
摘要： The present invention relates to a gene useful in a process to increase the microbial production of carotenoids. The carotenoids astaxanthin is distributed in a wide variety of organisms such as animals, algae and microorganisms. It has a strong antioxidation property against reactive oxygen species. Astaxanthin is used as a coloring reagent, especially in the industry of farmed fish, such as salmon, because astaxanthin imparts distinctive orange-red coloration to the animals and contributes to consumer appeal in the marketplace.
摘要翻译：本发明涉及可用于增加类胡萝卜素的微生物产生的方法中的基因。类胡萝卜素虾青素分布在各种生物体内，如动物，藻类和微生物。对活性氧具有很强的抗氧化性能。虾青素被用作着色试剂，特别是在养殖鱼类如鲑鱼的行业中，因为虾青素对动物施加独特的橙红色着色，并有助于消费者在市场上的吸引力。

3. 发明申请

WO2004029261A3 PRODUCTION OF CANTHAXANTHIN BY PHAFFIA 审中-公开
标题翻译：由PHAFFIA生产CANTHAXANTHIN
公开(公告)号：WO2004029261A3
公开(公告)日：2004-05-27
申请号：PCT/EP0310294
申请日：2003-09-16
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , OJIMA KAZUYUKI , SETOGUCHI YUTAKA
发明人： HOSHINO TATSUO , OJIMA KAZUYUKI , SETOGUCHI YUTAKA
IPC分类号： C12N9/02 , C12P23/00
CPC分类号： C12N9/0004 , C12P23/00
摘要： Disclosed is a process for producing canthaxanthin and echinenone which comprises cultivating a recombinant microorganism which is expressing a ss-carotene ketolase gene and belonging to the genus Xanthophyllomyces (Phaffia) in an aqueous nutrient medium under aerobic conditions, and isolating the resulted carotenoids from the cells of said recombinant microorganism or from the cultured broth.
摘要翻译：公开了一种角黄素和海胆烯酮的生产方法，包括在需氧条件下在含水营养培养基中培养表达β-胡萝卜素酮酶基因和属于叶黄霉属（法夫酵母属）的重组微生物，并从细胞中分离所得到的类胡萝卜素的所述重组微生物或来自培养的肉汤。

4. 发明申请

WO2004029260A3 ASTAXANTHIN PRODUCTION USING FED-BATCH FERMENTATION PROCESS BY PHAFFIA RHODOZYMA 审中-公开
标题翻译： PHAFFIA RHODOZYMA用FED-BATCH发酵工艺生产ASTAXANTHIN
公开(公告)号：WO2004029260A3
公开(公告)日：2004-05-27
申请号：PCT/EP0310293
申请日：2003-09-16
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , SETOGUCHI YUTAKA , TAKAGI YOSHINORI
发明人： HOSHINO TATSUO , SETOGUCHI YUTAKA , TAKAGI YOSHINORI
IPC分类号： C12M1/36 , C12P23/00
CPC分类号： C12P23/00
摘要： Disclosed is a fermentation method of astaxanthin using Phaffia rhodozyma comprising the steps of: (a) in the growing phase, feeding of a nutrient medium containing glucose or sucrose based on the specific growth rate (µ) of Phaffia rhodozyma cells, and (b) in the astaxanthin production phase, feeding of the nutrient medium based on the astaxanthin production rate, while keeping the glucose concentration in the fermentation broth almost 0 g/L during the whole fermentation period.
摘要翻译：本发明公开了一种使用红发夫酵母的虾青素发酵方法，包括以下步骤：（a）在生长阶段，根据红发夫酵母细胞的特定生长速率（μ）进料含有葡萄糖或蔗糖的营养培养基，和（b）在虾青素生产阶段，基于虾青素产生速率补充营养培养基，同时保持发酵培养基中的葡萄糖浓度在整个发酵期间几乎为0g / L。

5. 发明申请

WO2004029255A3 SQUALENE SYNTHASE 8SQS9 GEN 审中-公开
公开(公告)号：WO2004029255A3
公开(公告)日：2004-05-27
申请号：PCT/EP0310573
申请日：2003-09-23
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , OJIMA KAZUYUKI , SETOGUCHI YUTAKA
发明人： HOSHINO TATSUO , OJIMA KAZUYUKI , SETOGUCHI YUTAKA
IPC分类号： C12N9/10 , C12N15/81 , C12P23/00 , C12P235/00
CPC分类号： C12N9/1085 , C12N15/815 , C12P23/00
摘要： The present invention relates to a gene useful in a process to increase the microbial production of carotenoids. The carotenoids astaxanthin is distributed in a wide variety of organisms such as animals, algae and microorganisms. It has a strong antioxidation property against reactive oxygen species. Astaxanthin is used as a coloring reagent, especially in the industry of farmed fish, such as salmon, because astaxanthin imparts distinctive orange-red coloration to the animals and contributes to consumer appeal in the marketplace.
摘要翻译：本发明涉及可用于增加类胡萝卜素的微生物产生的方法中的基因。类胡萝卜素虾青素分布在各种生物体内，如动物，藻类和微生物。对活性氧具有很强的抗氧化性能。虾青素被用作着色试剂，特别是在养殖鱼类如鲑鱼的行业中，因为虾青素对动物施加独特的橙红色着色，并有助于消费者在市场上的吸引力。

6. 发明申请

WO2004029262A3 PRODUCTION OF 2 - KETO - L - GULONIC ACD 审中-公开
标题翻译：生产2 - KETO - L - GULONIC ACD
公开(公告)号：WO2004029262A3
公开(公告)日：2004-07-01
申请号：PCT/EP0310490
申请日：2003-09-22
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , SUGISAWA TERUHIDE , TAKAGI YOSHINORI
发明人： HOSHINO TATSUO , SUGISAWA TERUHIDE , TAKAGI YOSHINORI
IPC分类号： C12P7/60
CPC分类号： C12P7/60
摘要： The present invention relates to a process for the production of 2-KGA or a salt thereof from D-sorbitol by a multi-stage continuous or a single-stage semi-continuous fermentation mode using a mixed culture of microorganisms of the genus Gluconobacter or Acetobacter. One of the microorganisms is G. oxydans DSM 4025.
摘要翻译：本发明涉及使用葡萄糖酸杆菌属或醋酸杆菌属的微生物的混合培养的多阶段连续或单阶段半连续发酵模式从D-山梨糖醇生产2-KGA或其盐的方法。其中一种微生物是G.oxydans DSM 4025。

7. 发明申请

WO2004029252A3 A GENE ENCODING VITAMIN B6 PHOSPHATE PHOSPHATASE AND USE THEREOF 审中-公开
标题翻译：一种编码维生素B6磷酸酯酶的基因及其用途
公开(公告)号：WO2004029252A3
公开(公告)日：2004-06-03
申请号：PCT/EP0310575
申请日：2003-09-23
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , NAGAHASHI YOSHIE , TAZOE MASAAKI
发明人： HOSHINO TATSUO , NAGAHASHI YOSHIE , TAZOE MASAAKI
IPC分类号： C12N1/21 , C12N9/16 , C12N15/55 , C12P17/12
CPC分类号： C12N9/16 , C12P17/12
摘要： Disclosed is an isolated DNA encoding vitamin B6 phosphate phosphatase selected from the group consisting of: (a) a DNA sequence represented in SEQ ID NO:9; (b) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity and hybridizes under standard conditions to the DNA sequence defined in (a) or a fragment of thereof; (c) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity, wherein said polypeptide is at least 70 % identical to the amino acid sequence represented in SEQ ID NO:10; (d) a DNA sequence which encodes a polypeptide having vitamin B6 phosphate phosphatase activity and is at least 70 % identical to the DNA sequence represented in SEQ ID NO:9; (e) a degenerate DNA sequence of any one of (a) to (c).
摘要翻译：公开了编码选自以下的维生素B6磷酸磷酸酶的分离DNA：（a）SEQ ID NO：9所示的DNA序列; （b）编码具有维生素B6磷酸酯酶活性的多肽的DNA序列，并在标准条件下与（a）中定义的DNA序列或其片段杂交; （c）编码具有维生素B6磷酸酯酶活性的多肽的DNA序列，其中所述多肽与SEQ ID NO：10所示氨基酸序列至少70％相同; （d）编码具有维生素B6磷酸酯酶活性并与SEQ ID NO：9所示DNA序列至少70％相同的多肽的DNA序列; （e）（a）至（c）中任一项的简并DNA序列。

8. 发明申请

WO2004029265A3 PRODUCTION OF 2-KGA 审中-公开
标题翻译：生产2-KGA
公开(公告)号：WO2004029265A3
公开(公告)日：2004-05-27
申请号：PCT/EP0310488
申请日：2003-09-22
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , SUGISAWA TERUHIDE , TAKAGI YOSHINORI
发明人： HOSHINO TATSUO , SUGISAWA TERUHIDE , TAKAGI YOSHINORI
IPC分类号： C12P7/60 , C12P39/00 , C12N1/20
CPC分类号： C12P7/60 , C12P39/00
摘要： The present invention relates to a process for the production of 2-KGA from L-sorbose by a continuous or semi-continuous fermentation mode, wherein a mixed culture of Gluconobacter oxydans DSM 4025 with a second microorganism component is used, or wherein, instead of the second microorganism component, the fermentation medium is supplemented with yeast or a yeast product.
摘要翻译：本发明涉及由L-山梨糖通过连续或半连续发酵模式生产2-KGA的方法，其中使用氧化葡糖杆菌DSM 4025与第二微生物组分的混合培养物，或者其中代替第二微生物组分，发酵培养基补充有酵母或酵母产物。

9. 发明申请

WO2004029267A9 PROCESS FOR PRODUCING L-ASCORBIC ACID 审中-公开
标题翻译：生产L-抗坏血酸的方法
公开(公告)号：WO2004029267A9
公开(公告)日：2004-06-03
申请号：PCT/EP0310489
申请日：2003-09-22
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , SHINJOH MASAKO
发明人： HOSHINO TATSUO , SHINJOH MASAKO
IPC分类号： C12P7/58 , C12P17/04
CPC分类号： C12P7/58 , C12P17/04
摘要： The present invention provides the use of Enzyme B of G. oxydans DSM 4025, as disclosed in EP 0 832 974 A2, in a process for producing L-ascorbic acid from L-gulose, L- galactose, L-idose or L-talose, or from L-gulono-1,4-lactone (and its acid form, L-gulonic acid) and from L-galactono-1,4-lactone (and its acid form, L-galactonic acid).
摘要翻译：本发明提供了如EP 0 832 974 A2中公开的G.oxydans DSM 4025的酶B在从L-古洛糖，L-半乳糖，L-艾杜糖或L-塔罗糖生产L-抗坏血酸的方法中的用途，或由L-古洛糖酸-1,4-内酯（及其酸形式，L-古洛糖酸）和由L-半乳糖酸-1,4-内酯（及其酸形式，L-半乳糖酸）形成。

10. 发明申请

WO2004029270A3 PROCESS FOR PRODUCING VITAMIN B6 审中-公开
标题翻译：生产维生素B6的方法
公开(公告)号：WO2004029270A3
公开(公告)日：2004-05-27
申请号：PCT/EP0310296
申请日：2003-09-16
申请人： DSM IP ASSETS BV , HOSHINO TATSUO , NAGATANI YOICHIRO , ICHIKAWA KEIKO , TAZOE MASAAKI
发明人： HOSHINO TATSUO , NAGATANI YOICHIRO , ICHIKAWA KEIKO , TAZOE MASAAKI
IPC分类号： C12P17/12 , C12R1/41
CPC分类号： C12R1/41 , C12P17/12
摘要： Disclosed is a mutant of a recombinant microorganism of the genus Sinorhizobium capable of producing vitamin B6 having a recombinant plasmid with pdxJ gene that acquired histidine requirement of glycine resistance, or its combination thereof.
摘要翻译：公开了能够产生具有获得组氨酸需要甘氨酸抗性的pdxJ基因的重组质粒的维生素B6的重组微生物的突变体或其组合的突变体。

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