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    • 5. 发明申请
    • TRANSFORMATION SYSTEMS FOR FLAVINOGENIC YEAST
    • FLAVINOGENIC YEAST转化体系
    • WO0206448A3
    • 2003-05-15
    • PCT/US0122083
    • 2001-07-13
    • ABBAS CHARLES
    • ABBAS CHARLES
    • C07K14/40C12N15/01C12N15/81C12P25/00C12N15/00
    • C12R1/72C07K14/40C12N15/01C12N15/81C12P25/00
    • This invention is directed to the transformation of the flavinogenic yeasts, Pichia guilliermondii and Candida famata, and mutants therof, by electroporation (electrotransformation) and by spheroplast transformation. The invention is also directed to nucleic acid constructs such as vectors, plasmids, and ARS sequences which transform flavinogenic yeasts, and mutants thereof, at a high level and in a stable manner so as to result in stably transformed yeast host cells which express/produce recombinant products. This invention also is directed to flavinogenic yeasts, Pichia guilliermondii and Candida famata, and mutants and temperature sensitive mutants thereof, which produce or overproduce riboflavin.
    • 本发明涉及通过电穿孔(电转化)和通过原生质球转化转化黄素生物酵母,吉西州毕赤酵母和假丝酵母以及突变体。 本发明还涉及以高水平和稳定的方式转化黄素生物酵母及其突变体的载体,质粒和ARS序列的核酸构建体,从而产生稳定转化的酵母宿主细胞,其表达/产生 重组产物。 本发明还涉及产生或过量产生核黄素的黄素生成酵母,吉西州毕赤酵母和念珠菌,以及其突变体和温度敏感突变体。
    • 6. 发明申请
    • TRANSFORMATION SYSTEMS FOR FLAVINOGENIC YEAST
    • FLAVINOGENIC YEAST转化体系
    • WO02006448A2
    • 2002-01-24
    • PCT/US2001/022083
    • 2001-07-13
    • C07K14/40C12N15/01C12N15/81C12P25/00C12N1/00
    • C12R1/72C07K14/40C12N15/01C12N15/81C12P25/00
    • This invention is directed to the transformation of the flavinogenic yeasts, Pichia guilliermondii and Candida famata, and mutants therof, by electroporation (electrotransformation) and by spheroplast transformation. The invention is also directed to nucleic acid constructs such as vectors, plasmids, and ARS sequences which transform flavinogenic yeasts, and mutants thereof, at a high level and in a stable manner so as to result in stably transformed yeast host cells which express/produce recombinant products. This invention also is directed to flavinogenic yeasts, Pichia guilliermondii and Candida famata, and mutants and temperature sensitive mutants thereof, which produce or overproduce riboflavin.
    • 本发明涉及通过电穿孔(电转化)和通过原生质球转化转化黄素生物酵母,吉西州毕赤酵母和假丝酵母以及突变体。 本发明还涉及以高水平和稳定的方式转化黄素生物酵母及其突变体的载体,质粒和ARS序列的核酸构建体,从而产生稳定转化的酵母宿主细胞,其表达/产生 重组产物。 本发明还涉及产生或过量产生核黄素的黄素生成酵母,吉西州毕赤酵母和念珠菌,以及其突变体和温度敏感突变体。
    • 8. 发明申请
    • RIBOFLAVIN PRODUCING STRAINS OF MICROORGANISMS, METHOD FOR SELECTING, AND METHOD FOR FERMENTATION
    • 生产微生物菌株的RIBOFLAVIN,选择方法和发酵方法
    • WO1988009822A1
    • 1988-12-15
    • PCT/US1988001876
    • 1988-06-02
    • COORS BIOTECH PRODUCTS COMPANY
    • COORS BIOTECH PRODUCTS COMPANYHEEFNER, Donald, L.WEAVER, Craig, A.YARUS, Michael, J.BURDZINSKI, Linda, A.GYURE, Dale, C.FOSTER, Edward, W.
    • C12P25/00
    • C12N15/04C12N15/01C12P25/00C12R1/72Y10S435/921
    • Strains of yeast of the species Candida flareri are disclosed which can produce 10 grams of riboflavin per liter in 6 days, and in particular, strains identified by ATCC Accession Nos. 20849 and 20850. Riboflavin yields of more than 20 grams per liter in 200 hours have been achieved. Strains of the present invention have increased sensitivity to iron inhibition of flavinogenesis and have enhanced riboflavin production per cell at increased iron concentrations in the fermentation medium. The invention also is directed toward a process for selecting improved microorganisms which are resistant to inhibition of growth by depleted medium. Such selected microorganisms are then tested for riboflavin overproduction. The present invention is also directed toward a selection process in which mutated microorganisms are cultured in the presence of tubercidin, a purine analog. Mutant strains resistant to tubercidin are then tested for riboflavin overproduction. The present invention also includes a process for producing riboflavin by culturing strains of riboflavin overproducing microorganisms in a fermentation medium. Increased riboflavin production can be obtained by elevated iron concentrations in the nutrient medium. The fermentation is conducted and after cell growth levels off, riboflavin is recovered from the fermentation medium.
    • 公开了假丝酵母属的酵母菌株,其可以在6天内每升产生10克核黄素,特别是ATCC登录号20849和20850鉴定的菌株.200小时内每升的核黄素产量大于20克 已经实现。 本发明的菌株具有增加的铁抑制黄素生成的敏感性,并且在发酵培养基中铁浓度增加时,每个细胞的核黄素产量增加。 本发明还涉及一种选择改良的微生物的方法,所述改良的微生物对消耗的培养基抑制生长是有抗性的。 然后测试这样选择的微生物的核黄素过量产生。 本发明还涉及选择过程,其中在结核菌素嘌呤类似物存在下培养突变的微生物。 然后测试抗结核菌素的突变株,以检测核黄素过量产生。 本发明还包括通过在发酵培养基中培养过量生产微生物的核黄素菌株来生产核黄素的方法。 增加的核黄素生产可以通过营养培养基中升高的铁浓度获得。 进行发酵,在细胞生长水平以后,从发酵培养基中回收核黄素。