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1. 发明申请

WO2019085320A1 METHODS AND KITS FOR TARGETED ENRICHMENT OF TARGET DNA WITH HIGH GC CONTENT 审中-公开
公开(公告)号：WO2019085320A1
公开(公告)日：2019-05-09
申请号：PCT/CN2018/075543
申请日：2018-02-07
申请人： BERRY GENOMICS CO., LTD
发明人： QU, Ziwei , YU, Tao , WANG, Fangming , ZHANG, Nannan , ZHANG, Jianguang
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6806 , C12Q2537/159 , C12Q2565/519
摘要： The present invention provides a method for enrichment of target DNA with high GC content based on target sequence capture and multiple displacement amplification, as well as a kit suitable for this method. The present invention also provides a method for constructing a sequencing library of target DNA with high GC content based on the enrichment method of the present invention.

2. 发明申请

WO2019060722A2 METHODS AND COMPOSITIONS FOR PREPARING POLYNUCLEOTIDES 审中-公开
公开(公告)号：WO2019060722A2
公开(公告)日：2019-03-28
申请号：PCT/US2018/052217
申请日：2018-09-21
申请人： X GEN US CO.
发明人： WU, Yalei , LEE, Wai Ho , LAO, Kai Qin
IPC分类号： C12Q1/686
CPC分类号： C12Q1/6806 , C12Q2523/125 , C12Q2537/159
摘要： Provided herein are methods, compositions, and kits for forming amplification products. In various embodiments provided herein, transposomes comprising transposases are used in forming tagged polynucleotides for downstream amplification and polynucleotide processing steps.

3. 发明申请

WO2016207379A1 TARGETED ENRICHMENT OF LONG NUCLEOTIDE SEQUENCES USING MICROFLUIDIC PARTITIONING 审中-公开
标题翻译：使用微流控制的长核序列的目标强化
公开(公告)号：WO2016207379A1
公开(公告)日：2016-12-29
申请号：PCT/EP2016/064719
申请日：2016-06-24
申请人： SAMPLIX S.A.R.L.
发明人： MIKKELSEN, Marie Just , KVIST, Thomas , MADSEN, Esben Bjørn
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6806 , C12Q1/686 , C12Q2521/531 , C12Q2535/122 , C12Q2537/159 , C12Q2561/101 , C12Q2563/159 , C12Q2565/629
摘要： The invention relates to a method for enrichment or isolation of a long nucleotide fragment comprising a known nucleotide sequence element, i.e. a sequence encoding a conserved active site or domain, the method being applicable i.a. to high throughput screening for DNA fragments containing a known sequence element.
摘要翻译：本发明涉及用于富集或分离长核苷酸片段的方法，所述长核苷酸片段包含已知的核苷酸序列元件，即编码保守活性位点或结构域的序列，该方法适用于此。以高通量筛选含有已知序列元件的DNA片段。

4. 发明申请

WO2016161054A1 MASSIVE PARALLEL PRIMER DIMER-MEDIATED MULTIPLEXED SINGLE CELL-BASED AMPLIFICATION FOR CONCURRENT EVALUATION OF MULTIPLE TARGET SEQUENCES IN COMPLEX CELL MIXTURES 审中-公开
标题翻译：大容量并行引导二进制多媒体多单一单元基于扩频的多重目标序列在复合单元混合中的并行评估
公开(公告)号：WO2016161054A1
公开(公告)日：2016-10-06
申请号：PCT/US2016/025124
申请日：2016-03-31
申请人： PHARMACYCLICS LLC
发明人： SCHOENMAKERS, Henricus Franciscus Petrus, Maria
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12N15/09 , C12N15/1031 , C12Q1/68 , C12Q1/6853 , C12Q2525/161 , C12Q2525/197 , C12Q2531/113 , C12Q2537/143 , C12Q2537/159
摘要： The present invention provides methods and compositions for parallel single cell-based multiplexed primer dimer-mediated amplification of multiple target sequences in complex cell mixtures. The invention provides primer dimer-mediated concatenation of multiple targets in multi-target fusion amplicon products for evaluation of two or more target sequences as present individual cells across a population or mixture of cells.
摘要翻译：本发明提供了用于复杂细胞混合物中多个靶序列的并行单细胞复合引物二聚体介导扩增的方法和组合物。本发明提供了多靶标融合扩增子产物中多个靶点的引物二聚体介导的连接，用于评估两个或更多个目标序列作为跨细胞群体或混合物存在的个体细胞。

5. 发明申请

WO2016090273A1 MULTIGENE ANALYSIS OF TUMOR SAMPLES 审中-公开
标题翻译：肿瘤样本的多元分析
公开(公告)号：WO2016090273A1
公开(公告)日：2016-06-09
申请号：PCT/US2015/064044
申请日：2015-12-04
申请人： FOUNDATION MEDICINE, INC.
发明人： OTTO, Geoffrey, Alan , NAHAS, Michelle , LIPSON, Doron
IPC分类号： C12Q1/68 , C40B20/00 , C40B20/04 , C40B40/06 , C40B50/00 , A61K39/395
CPC分类号： C12Q1/6886 , C12N15/1065 , C12Q1/6806 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G06F19/20 , G06F19/22 , G06F19/26 , C12Q2535/122 , C12Q2537/159 , C12Q2563/131
摘要： Methods of evaluating or providing a clonal profile of a subject interval, e.g., a subgenomic interval, or an expressed subgenomic interval (or of a cell containing the same), in a subject, are disclosed.
摘要翻译：公开了评价或提供受试者间隔的克隆分布的方法，例如亚基因组间隔或表达的亚基因组间隔（或包含其的细胞）。

6. 发明申请

WO2015165667A1 PHAGE DISPLAY LIBRARY 审中-公开
标题翻译： PHAGE显示库
公开(公告)号：WO2015165667A1
公开(公告)日：2015-11-05
申请号：PCT/EP2015/056677
申请日：2015-03-27
申请人： FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
发明人： SZARDENINGS, Michael , DELAROQUE, Nicolas
IPC分类号： C12N15/10 , C07K1/04
CPC分类号： C12N15/1037 , C12Q2525/179 , C12Q2537/159
摘要： A library of replicating entities, each entity comprises a recombinant vector comprising a randomized nucleic acid sequence, having the reading frame structure [NXX] n [CorAA] [NXX] m [NZZ] o , or [NZZ] o [NXX] m [CorAA] [NXX] n . Each NXX is independently a codon encoding for any amino acid except cysteine, CorAA is a codon encoding for cysteine or at least one other amino acid, each NZZ is independently a codon encoding for any amino acid, and n is an integer from 0 to 40, m is an integer from 1 to 20, o is an integer from 1 to 40, and at least 20 percent of CorAA encode for cysteine. The invention further relates to a set of recombinant vectors and to a set of randomized oligonucleotides, each oligonucleotide having said structure, as well as to a method for generating a library of replicating entities and to a method for identifying an amino acid polymer.
摘要翻译：复制实体文库，每个实体包含重组载体，其包含具有阅读框架结构[NXX] n [CorAA] [NXX] m [NZZ] o或[NZZ] o [NXX] m [ CorAA] [NXX] n。每个NXX独立地是编码除半胱氨酸之外的任何氨基酸的密码子，CorAA是编码半胱氨酸或至少一个其他氨基酸的密码子，每个NZZ独立地是编码任何氨基酸的密码子，并且n是0至40的整数 m为1〜20的整数，o为1〜40的整数，至少20％的CorAA编码为半胱氨酸。本发明还涉及一组重组载体和一组随机寡核苷酸，每个寡核苷酸具有所述结构，以及用于产生复制实体文库的方法和用于鉴定氨基酸聚合物的方法。

7. 发明申请

WO2015132615A1 ENRICHING A SAMPLE OF CIRCULATING CELL-FREE NUCLEIC ACIDS 审中-公开
标题翻译：增加循环无细胞核酸的样品
公开(公告)号：WO2015132615A1
公开(公告)日：2015-09-11
申请号：PCT/GB2015/050681
申请日：2015-03-09
申请人： OXFORD GENE TECHNOLOGY (OPERATIONS) LTD. , UCL BUSINESS PLC
发明人： SOUTHERN, Edwin , HURD, Douglas , LUEERSSEN, Dietrich , REID, James , CHITTY, Lyn
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6851 , C12Q1/6855 , C12Q1/686 , C12Q1/6883 , C12Q1/6886 , C12Q2525/191 , C12Q2537/159
摘要： A method of enriching a sample of circulating cell-free DNA for DNA derived from a foetus or from a tumour includes carrying out amplification of DNA such that amplification of longer DNA molecules is discriminated against. This results in preferential amplification of foetal or tumour DNA, which can then be analysed using, for example, array comparative genome hybridisation.
摘要翻译：富含来自胎儿或来自肿瘤的DNA的循环的无细胞DNA样品的方法包括进行DNA的扩增，以便区分更长的DNA分子的扩增。这导致胎儿或肿瘤DNA的优先扩增，然后可以使用例如阵列比较基因组杂交来分析。

8. 发明申请

WO2014196863A1 Method for targeted sequencing 审中-公开
标题翻译：靶向测序方法
公开(公告)号：WO2014196863A1
公开(公告)日：2014-12-11
申请号：PCT/NL2014/050369
申请日：2014-06-06
申请人： KEYGENE N.V.
发明人： HOGERS, René Cornelis Josephus
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6869 , C12Q1/6855 , C12Q2521/501 , C12Q2525/161 , C12Q2525/191 , C12Q2525/197 , C12Q2525/307 , C12Q2531/125 , C12Q2535/122 , C12Q2537/159 , C12Q2563/179
摘要： The method of the present invention now provides a technique for generating sequence information from nucleic acid samples based on knowledge from part(s) of the nucleotide sequence. The knowledge of the partial sequence may include knowledge about the presence of restriction sites. The knowledge of the partial sequence can be used to generate adaptor-ligated or nucleotide–elongated fragments. From the combination of information on the ligated adaptor and the Known Nucleotide Sequence Section,probes can be designed. The probes can be used in the provision of circularised fragments that can be sequenced. Combining the known and determined sequences adds sequence information to the already existing sequence information and complements the available genomic sequence information.
摘要翻译：本发明的方法现在提供了一种基于核苷酸序列的一部分的知识从核酸样品产生序列信息的技术。部分序列的知识可以包括关于限制性位点的存在的知识。部分序列的知识可用于产生衔接子连接或核苷酸延伸的片段。从连接适配器和已知核苷酸序列部分的信息的组合可以设计探针。探针可用于提供可排序的循环碎片。结合已知和确定的序列将序列信息添加到已经存在的序列信息中并补充可用的基因组序列信息。

9. 发明申请

WO2014186152A1 ANALYTE ENRICHMENT METHODS AND COMPOSITIONS 审中-公开
标题翻译：分析方法和组合
公开(公告)号：WO2014186152A1
公开(公告)日：2014-11-20
申请号：PCT/US2014/036591
申请日：2014-05-02
申请人： INTELLIGENT BIO-SYSTEMS, INC.
发明人： OLEJNIK, Jerzy , GORDON, Steve , WERNER, Martina
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6844 , C12Q2537/159 , C12Q2563/131 , C12Q2563/149 , C12Q2563/159
摘要： Methods and compositions for enriching a population of particles containing an analyte are disclosed. In one embodiment, enrichment beads are used that are larger in size than the beads used for amplification. A separation device is employed that can retain larger beads with bound amplified beads. The technique finds many uses, including enriching for beads with clonally amplified template, which can be used in a variety of assays, including nucleic acid sequencing.
摘要翻译：公开了用于富集含有分析物的颗粒群的方法和组合物。在一个实施方案中，使用比用于扩增的珠更大的富集珠。使用分离装置，其可以用结合的扩增珠粒保留较大的珠粒。该技术发现许多用途，包括用克隆扩增模板富集珠，可用于多种测定，包括核酸测序。

10. 发明申请

WO2014172529A1 METHODS FOR CHARACTERIZING DNA SEQUENCE COMPOSITION IN A GENOME 审中-公开
标题翻译：表征基因组DNA序列组成的方法
公开(公告)号：WO2014172529A1
公开(公告)日：2014-10-23
申请号：PCT/US2014/034476
申请日：2014-04-17
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , BEATTY, Mary , HAYES, Kevin Robert , HOFFMAN, Jenna Lynn , LIN, Haining , ZASTROW-HAYES, Gina Marie
发明人： BEATTY, Mary , HAYES, Kevin Robert , HOFFMAN, Jenna Lynn , LIN, Haining , ZASTROW-HAYES, Gina Marie
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q2525/191 , C12Q2535/122 , C12Q2537/159
摘要： Methods for the high-throughput analysis of transgenic events are herein disclosed. The methods use libraries of sheared genomic DNA ligated to specialized adapters and pooled for sequence analysis and comparison to known genomic and insert sequence. The method finds use in detecting characterizing insertion site, transgene integrity, and transgene copy number.
摘要翻译：本文公开了转基因事件的高通量分析方法。该方法使用剪切的基因组DNA连接到专门的衔接子并汇集进行序列分析并与已知的基因组和插入序列进行比较。该方法用于检测表征插入位点，转基因完整性和转基因拷贝数。

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