专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2022190014A1 HIGH THROUGHOUT SCREENING IN DROPLETS 审中-公开
公开(公告)号：WO2022190014A1
公开(公告)日：2022-09-15
申请号：PCT/IB2022/052124
申请日：2022-03-09
申请人： NOVARTIS AG
发明人： ABEYWARDANE, Asitha Adrian , APSUNDE, Tushar Dattu , ETIENNE, Gianluca Nicolas , GAMBARDELLA, Alessia , MAINQUIST, James Kevin , MEYER, Andrew J. , MINIE, Brian , PAULA, Steven , VICKERS, Dwayne A. L. , YAMADA, Ken
IPC分类号： B01F23/41 , B01F25/43 , B01F25/433 , B01F33/30 , B01J19/00 , B01L3/00 , C40B40/00 , C40B50/00 , C08G81/00 , C08G65/00 , C08G65/332 , C08G65/337 , C08L87/00 , C09K23/00
摘要： The present disclosure generally relates to compositions, methods, sorters, systems, devices and uses for screening for bioactive substances in emulsion droplets. In some embodiments, the compositions are a continuous phase formulation for stable emulsions, In some embodiments, the methods are for preparing a monodisperse polyethylene glycol acrylamide (PEGA) co-polymer resin, or for preparing a core-shell bead. In some embodiments, the systems and devices include a sorter, comprising an inlet channel, first and second outlet channels meeting the inlet channel at a junction, and first and second electrodes proximate respective first and second sides of the junction sorter. In some embodiments, the systems and devices include a sorter, comprising a microwell array plate configured to host one microdroplet per microwell, a fluorescence microscope, an imager configured to automatically image assay droplets and identify desired droplets, and an automated microcapillary-based droplet sampling device configured to continuously deposit desired droplets to hit wells.

2. 发明申请

WO2021191247A1 NEW METHOD FOR AUTOMATED ON-DEMAND BIOMOLECULAR ARRAY SYNTHESIS 审中-公开
公开(公告)号：WO2021191247A1
公开(公告)日：2021-09-30
申请号：PCT/EP2021/057520
申请日：2021-03-24
申请人： TECHNISCHE UNIVERSITÄT DRESDEN
发明人： ZHANG, Yixin , LIN, Weilin
IPC分类号： G01N33/543 , B01J21/00 , B01J19/0046 , C40B50/00 , G01N33/54393
摘要： The invention provides an amphiphilic coating for the direct and rapid synthesis of an array of peptides and small molecular compounds on a planar surface of a solid support, comprising a hydrophilic chemical structure and a lipophilic group, wherein said peptides and small molecular compounds differ from spot to spot from each other in the chemical structure, characterized in that said amphiphilic coating possesses low wettability to polar aprotic solvents used in the array synthesis; said amphiphilic coating possessing low wettability is designed that it can be converted to a coating possessing high wettability by hydrolysis of the lipophilic group; and said amphiphilic coating comprises an amino group for the reaction with an electrophilic reagent. The invention further provides a solid support comprising said amphiphilic coating and a method for method for the direct and rapid synthesis of an array of peptides and small molecular compounds on a planar surface of a solid support, wherein said planar surface of a solid support comprises said amphiphilic coating. Said method includes the enhancing of the wettability of a glass surface to organic solvents to realize automated on-demand biomolecular array synthesis comprising both, peptides and small molecular compounds. The amphiphilic surface can be switched to a hydrophilic surface, resulting in high density arrays suitable for protein- and cell-based screening.

3. 发明申请

WO2019222612A1 DEVICES, SYSTEMS, AND METHODS OF ELECTRONIC MODULATION OF POLYMERASE FOR DNA SYNTHESIS 审中-公开
公开(公告)号：WO2019222612A1
公开(公告)日：2019-11-21
申请号：PCT/US2019/032844
申请日：2019-05-17
申请人： GEORGIA TECH RESEARCH CORPORATION
发明人： WANG, Hua , HESSEL, Andrew
IPC分类号： C07H21/04 , C40B50/18 , C40B50/14 , C40B50/00 , C12N15/09
摘要： A method of synthesis of a nucleotide chain, the nucleotide chain including an ordered plurality of nucleotides, the method including: identifying a first nucleotide of the ordered plurality of nucleotides; controlling a polymerase enzyme to assemble the first nucleotide onto the nucleotide chain by electrically modulating an electrode; identifying a subsequent nucleotide in the ordered plurality of nucleotides as a current nucleotide; and controlling the polymerase enzyme to assemble the current nucleotide onto an end of the nucleotide chain by electrically modulating the electrode.

4. 发明申请

WO2019157693A1 DNA编码分子库及应用广的化合物筛选方法审中-公开
公开(公告)号：WO2019157693A1
公开(公告)日：2019-08-22
申请号：PCT/CN2018/076803
申请日：2018-02-14
申请人：深圳劲宇生物科技有限公司
发明人：韩珂珩 , 托斯特·迪恩 , 赵劲 , 黄湧 , 周海鹏 , 李笑宇
IPC分类号： C40B40/00 , C40B30/04 , C40B50/00 , C40B70/00
摘要：一种DNA编码分子库，包括DNA标签，该标签包括第一引物区和第二引物区，第一引物区一端连接有化合物，DNA编码分子库还包括短链DNA和夹板DNA，短链DNA的一端与第一引物区结合，短链DNA的另一端与夹板DNA通过碱基配对结合，且夹板DNA与DNA标签通过碱基配对结合；短链DNA在靠近化合物的一端连接有光交联基团。一种DNA编码分子库的化合物筛选方法：将上述DNA编码分子库中加入DNA连接酶进行连接处理，然后与蛋白质靶点孵育后光照处理；将光照处理后的产物进行电泳分离，收集与蛋白质靶点结合的DNA标签并进行DNA测序，以筛选出对应的化合物。该方法够用于任何蛋白质靶点的DNA编码分子库的筛选。

5. 发明申请

WO2019148037A1 NANOBODY BASED IMAGING AND TARGETING OF ECM IN DISEASE AND DEVELOPMENT 审中-公开
公开(公告)号：WO2019148037A1
公开(公告)日：2019-08-01
申请号：PCT/US2019/015290
申请日：2019-01-25
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人： HYNES, Richard, O. , JAILKHANI, Noor
IPC分类号： C07K16/18 , A61K39/395 , C07K16/22 , C07K14/78 , G01N33/53 , C12N15/10 , A61P37/00 , A61P9/10 , A61P35/00 , C40B50/00 , C40B40/08 , G01N33/68 , G01N33/574
摘要： Methods for developing disease-related nanobodies and related products and kits are provided. The disease- specific proteins are extracellular matrix (ECM) proteins, domains or epitopes that are associated with various aspects of disease and are not present, or are present in very low quantities, in non-diseased individuals. Highly effective nanobodies capable of specifically binding to these ECM protein epitopes useful in in vivo imaging assays, the detection, diagnosis and treatment of diseases as well as monitoring therapeutic progress in a patient with a disease are provided herein.

6. 发明申请

WO2017197488A1 LIBRARIES OF DIVERSE MACROCYCLIC COMPOUNDS AND METHODS OF MAKING AND USING THE SAME 审中-公开
标题翻译：多种大环化合物的库及其制备和使用方法
公开(公告)号：WO2017197488A1
公开(公告)日：2017-11-23
申请号：PCT/CA2017/000128
申请日：2017-05-16
申请人： CYCLENIUM PHARMA INC.
发明人： MACDONALD, Dwight , DUBÉ, Daniel , WAHHAB, Amal , THOMAS, Helmut , RICHARD, Luc , PETERSON, Mark L.
IPC分类号： C40B40/04 , C40B30/00 , C40B30/04 , C40B30/06 , C40B30/08 , C40B50/00 , G01N33/48 , C12Q1/00
摘要： The present disclosure relates to novel macrocyclic compounds and libraries thereof that are useful as research tools for drug discovery efforts. This disclosure also relates to methods of preparing these compounds and libraries and methods of using these libraries, such as in high throughput screening. In particular, these libraries are useful for evaluation of bioactivity at existing and newly identified pharmacologically relevant targets, including G protein-coupled receptors, nuclear receptors, enzymes, ion channels, transporters, transcription factors, protein-protein interactions and nucleic acid-protein interactions. As such, these libraries can be applied to the search for new pharmaceutical agents for the treatment and prevention of a range of medical conditions.
摘要翻译：本公开涉及新的大环化合物及其文库，其可用作用于药物发现工作的研究工具。本公开还涉及制备这些化合物和文库的方法以及使用这些文库的方法，例如高通量筛选。特别是，这些文库可用于评估现有和新鉴定的药理学相关靶点的生物活性，包括G蛋白偶联受体，核受体，酶，离子通道，转运蛋白，转录因子，蛋白质 - 蛋白质相互作用和核酸 - 蛋白质相互作用。因此，这些文库可以用于寻找治疗和预防一系列医疗状况的新药物。

7. 发明申请

WO2016109808A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID-TEMPLATED SYNTHESIS OF LARGE LIBRARIES OF COMPLEX SMALL MOLECULES 审中-公开
标题翻译：用于复合小分子的大型图谱的核酸酸化合成的方法和组合物
公开(公告)号：WO2016109808A1
公开(公告)日：2016-07-07
申请号：PCT/US2015/068308
申请日：2015-12-31
申请人： ENSEMBLE THERAPEUTICS CORPORATION
发明人： TERRETT, Nicholas, K. , CONNORS, William, H.
IPC分类号： C40B50/00
CPC分类号： C40B50/00 , C12N15/1031
摘要： The present invention provides methods and compositions for expanding the scope of chemical reactions that can be performed during nucleic acid-templated organic syntheses, and for producing small molecule libraries of greater size, scope and complexity than previously possible.
摘要翻译：本发明提供了用于扩展可在核酸模板化有机合成过程中进行的化学反应范围以及用于产生比以前可能的更大尺寸，范围和复杂性的小分子文库的方法和组合物。

8. 发明申请

WO2015079053A3 SYNTHETIC PROMOTERS FOR CHO CELLS, METHODS OF PRODUCING SYNTHETIC PROMOTERS USING TRANSCRIPTION FACTOR BINDING SITE MODULES 审中-公开
标题翻译：用于CHO细胞的合成促进剂，使用转录因子结合位点模块生产合成促进剂的方法
公开(公告)号：WO2015079053A3
公开(公告)日：2015-10-08
申请号：PCT/EP2014076024
申请日：2014-11-28
申请人： UCB BIOPHARMA SPRL
发明人： JAMES DAVID CAMERON , BROWN ADAM JOHN
IPC分类号： C12N15/85 , C40B50/00
CPC分类号： C12N15/85 , C12N15/1058 , C12N2800/24 , C12N2800/40 , C12N2830/00 , C12N2830/008 , C12N2830/15
摘要： CHO cell-specific synthetic promoter constructs for expressing recombinant proteins, a library of promoter constructs thereof, and a method for producing the promoter constructs. The promoter constructs enable precise control of recombinant gene transcription over three orders of magnitude, with the top expressing promoters capable of double the transcriptional activity of the CMV promoter.
摘要翻译：用于表达重组蛋白质的CHO细胞特异性合成启动子构建体，其启动子构建体的文库和用于产生启动子构建体的方法。启动子构建体能够精确控制三个数量级的重组基因转录，顶部表达启动子能够使CMV启动子的转录活性增加一倍。

9. 发明申请

WO2014190214A1 TARGETED TRANSPOSITION FOR USE IN EPIGENETIC STUDIES 审中-公开
标题翻译：针对传统研究使用的传染性疾病
公开(公告)号：WO2014190214A1
公开(公告)日：2014-11-27
申请号：PCT/US2014/039250
申请日：2014-05-22
申请人： ACTIVE MOTIF, INC.
发明人： JELINEK, Mary, Anne , EGAN, Brian, Stanley , FERNANDEZ, Joseph , SAMUELSSON, Johanna
IPC分类号： C12N15/10 , C07H21/00 , C40B50/00 , C40B40/06
CPC分类号： C12N15/1068 , C07H21/00 , C12N15/1006 , C12N15/1034 , C12N15/1065 , C12N15/1093 , C12Q1/6804 , C12Q1/6806 , C12Q2522/101 , C12Q2535/122
摘要： Disclosed herein are compositions, methods and kits useful for epigenetic analysis based on the use of transposons that are targeted to specific regions of chromatin based on DNA-DNA interactions, protein-protein interactions, RNA-RNA interactions, and nucleic acid-protein interactions.
摘要翻译：本文公开了用于基于使用基于DNA-DNA相互作用，蛋白质 - 蛋白质相互作用，RNA-RNA相互作用和核酸 - 蛋白质相互作用的染色质特定区域的转座子的表观遗传学分析的组合物，方法和试剂盒。

10. 发明申请

WO2013054200A3 METHODS AND COMPOSITIONS FOR SCREENING AND TREATING DEVELOPMENTAL DISORDERS 审中-公开
标题翻译：筛选和治疗发展障碍的方法和组合
公开(公告)号：WO2013054200A3
公开(公告)日：2013-08-22
申请号：PCT/IB2012002498
申请日：2012-10-10
申请人： HOSPITAL FOR SICK CHILDREN , SCHERER STEPHEN
发明人： SCHERER STEPHEN
IPC分类号： C12N15/12 , C07H21/00 , C12Q1/02 , C12Q1/68 , C40B30/00 , C40B30/04 , C40B40/06 , C40B40/08 , C40B50/00 , C40B50/14 , G01N33/50 , G01N33/53
CPC分类号： C12Q1/6883 , C07K14/47 , C07K14/4728 , C07K14/705 , C12N9/20 , C12N9/93 , C12Q2600/156 , G01N33/6896 , G01N2500/04 , G01N2800/2814
摘要： This document provides methods and materials related to genetic variations of developmental disorders. For example, this document provides methods for using such genetic variations to assess susceptibility of developing Autism Spectrum Disorder.
摘要翻译：本文件提供了与发育障碍遗传变异相关的方法和材料。例如，本文件提供了使用这种遗传变异来评估发展中的自闭症谱系障碍的易感性的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式