基本信息:
- 专利标题： ARTIFICIAL OOCYTE ACTIVATION
- 专利标题（中）：人工活动
- 申请号：PCT/US2014/067588 申请日：2014-11-26
- 公开(公告)号：WO2015081182A1 公开(公告)日：2015-06-04
- 发明人： LEE, Kiho , PRATHER, Randall, S.
- 申请人： THE CURATORS OF THE UNIVERSITY OF MISSOURI
- 申请人地址： 316 University Hall Columbia, MO 65211 US
- 专利权人： THE CURATORS OF THE UNIVERSITY OF MISSOURI
- 当前专利权人： THE CURATORS OF THE UNIVERSITY OF MISSOURI
- 当前专利权人地址： 316 University Hall Columbia, MO 65211 US
- 代理机构： HANSON, Robert, E.
- 优先权： US61/997,176 20140523; US61/963,246 20131127
- 主分类号： C12N5/075
- IPC分类号： C12N5/075 ; C12N15/877
摘要：
The present invention provides novel methods for improving the efficiency of artificial activation of unfertilized mammalian oocytes by reducing the intracellular concentration of Zn 2+ in the oocyte. The methods of the invention may additionally comprise a preceding step of increasing the intracellular concentration of Ca 2+ in the oocyte prior to reduction of the intracellular Zn 2+ concentration. The invention further provides unfertilized oocytes activated by the disclosed methods and viable mammalian animals produced from unfertilized oocytes activated by the disclosed methods.
摘要（中）：
本发明提供了通过降低卵母细胞中Zn 2+的细胞内浓度来提高未受精哺乳动物卵母细胞人工活化效率的新方法。 本发明的方法可以另外包括在减少细胞内Zn 2+浓度之前增加卵母细胞中Ca 2+的细胞内浓度的前述步骤。 本发明还提供了通过所公开的方法活化的未受精的卵母细胞和由所公开的方法激活的未受精的卵母细胞产生的活的哺乳动物。