发明申请
WO2011132990A2 TRANSGENIC CLONED CANINE OVEREXPRESSING PEPCK, AND PRODUCTION METHOD THEREOF
审中-公开
基本信息:
- 专利标题： TRANSGENIC CLONED CANINE OVEREXPRESSING PEPCK, AND PRODUCTION METHOD THEREOF
- 专利标题（中）：转基因克隆重组棉花生产方法及其生产方法
- 申请号：PCT/KR2011002944 申请日：2011-04-22
- 公开(公告)号：WO2011132990A2 公开(公告)日：2011-10-27
- 发明人： HWANG WOO-SUK
- 申请人： HWANG WOO-SUK
- 专利权人： HWANG WOO-SUK
- 当前专利权人： HWANG WOO-SUK
- 优先权： KR20100037299 2010-04-22
- 主分类号： C12N5/075
- IPC分类号： C12N5/075 ; A01K67/027 ; C12N5/16 ; C12N15/873
摘要：
The present invention relates to a transgenic cloned canine overexpressing phosphoenol pyruvate carboxykinase (PEPCK), and a production method thereof. More specifically, the present invention relates to a nuclear transferred egg of a canine formed by transferring the nucleus of a somatic cell derived from a canine transformed with a PEPCK gene to an enucleated egg, a production method thereof, a transgenic cloned canine overexpressing PEPCK produced by transferring the nuclear transferred egg into the oviduct of a surrogate mother, and a production method thereof. The transgenic cloned canine overexpressing PEPCK gene according to the present invention, as an animal model of diabetes mellitus, can be used for investigating the genetic cause of type 2 diabetes mellitus and developing a diagnostic reagent and a therapeutic agent.
摘要（中）：
本发明涉及转基因克隆犬过表达磷酸烯醇丙酮酸羧激酶（PEPCK）及其制备方法。 更具体地，本发明涉及通过将衍生自用PEPCK基因转化的犬的体细胞的细胞核转移到去核卵而形成的犬的核转移卵，其生产方法，产生过表达PEPCK的转基因克隆犬 通过将核转移的卵转移到替代母亲的输卵管中，及其制造方法。 作为糖尿病的动物模型的本发明的过表达PEPCK基因的转基因克隆犬可用于研究2型糖尿病的遗传原因并开发诊断试剂和治疗剂。