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1. 发明申请

WO1997021814A1 ANTIFUNGAL PROTEINS 审中-公开
标题翻译：抗真菌蛋白
公开(公告)号：WO1997021814A1
公开(公告)日：1997-06-19
申请号：PCT/GB1996003065
申请日：1996-12-12
申请人： ZENECA LIMITED , BROEKAERT, Willem, Frans , DE SAMBLANX, Genovera, Wivina , REES, Sarah, Bronwen
发明人： ZENECA LIMITED
IPC分类号： C12N15/29
CPC分类号： C12N15/8282 , A01N65/08 , C07K14/415
摘要： Antifungal proteins which are analogues of the Rs-AFP2 protein and contain particular mutations in their amino acid sequence. The mutated proteins possess enhanced salt-tolerant antifungal activity. The proteins are useful for combating fungal diseases in agricultural, pharmaceutical or preservative applications.
摘要翻译：抗真菌蛋白是Rs-AFP2蛋白的类似物，并在其氨基酸序列中含有特定的突变。突变蛋白具有增强的耐盐性抗真菌活性。该蛋白质可用于防治农业，药物或防腐应用中的真菌疾病。

2. 发明申请

WO1995004754A1 ANTIMICROBIAL PROTEINS 审中-公开
标题翻译：抗微生物蛋白
公开(公告)号：WO1995004754A1
公开(公告)日：1995-02-16
申请号：PCT/GB1994001636
申请日：1994-07-29
申请人： ZENECA LIMITED , BROEKAERT, Willem, Frans , CAMMUE, Bruno, Philippe, Angelo , REES, Sarah, Bronwen
发明人： ZENECA LIMITED
IPC分类号： C07K14/415
CPC分类号： C12N15/8282 , A01N65/42 , C07K14/415 , C12N15/8281
摘要： Antimicrobial proteins capable of isolation from seeds of Allium show a wide range of antifungal activity and some activity against Gram-positive bacteria. DNA encoding the proteins may be isolated and incorporated into vectors. Plants transformed with this DNA may be produced. The proteins find commercial application as antifungal or antibacterial agents; transformed plants will show increased disease resistance.
摘要翻译：能够从葱属种子中分离的抗微生物蛋白质具有广泛的抗真菌活性和一些抗革兰氏阳性菌的活性。编码蛋白质的DNA可以被分离并并入载体中。可以生产用该DNA转化的植物。蛋白质作为抗真菌剂或抗菌剂找到商业应用; 转化的植物将显示增加的抗病性。

3. 发明申请

WO1995024486A1 ANTIMICROBIAL PROTEINS FROM ARALIA AND IMPATIENS 审中-公开
标题翻译：来自阿拉伯和不丹的抗微生物蛋白质
公开(公告)号：WO1995024486A1
公开(公告)日：1995-09-14
申请号：PCT/GB1995000509
申请日：1995-03-09
申请人： ZENECA LIMITED , ATTENBOROUGH, Sheila , BROEKAERT, Willem, Frans , OSBORN, Rupert, William , RAY, John, Anthony , REES, Sarah, Bronwen , TAILOR, Ravindra, Haribhai
发明人： ZENECA LIMITED
IPC分类号： C12N15/29
CPC分类号： C12N15/8282 , A01N65/08 , C07K14/415 , C07K2319/02 , C12N15/62 , C12N15/8216 , C12N15/8281
摘要： Antimicrobial proteins capable of isolation from seeds of Aralia or Impatiens show a wide range of antifungal activity and some antibacterial activity. DNA encoding the proteins may be isolated and incorporated into vectors. Plants transformed with this DNA may be produced. The proteins find commercial application as antifungal or antibacterial agents; transformed plants will show increased disease-resistance. The invention further provides a method of expressing polyproteins in transgenic plants using DNA constructs based on the structure of the gene encoding the Impatiens antimicrobial proteins.
摘要翻译：能够分离Aralia或Impatiens种子的抗菌蛋白质显示出广泛的抗真菌活性和一些抗菌活性。编码蛋白质的DNA可以被分离并并入载体中。可以生产用该DNA转化的植物。蛋白质作为抗真菌剂或抗菌剂找到商业应用; 转化的植物将显示增加的抗病性。本发明还提供了一种使用基于编码凤仙花抗微生物蛋白的基因结构的DNA构建体在转基因植物中表达多种蛋白质的方法。

4. 发明申请

WO1997021815A2 ANTIFUNGAL PROTEINS 审中-公开
标题翻译：抗真菌蛋白
公开(公告)号：WO1997021815A2
公开(公告)日：1997-06-19
申请号：PCT/GB1996003068
申请日：1996-12-12
申请人： ZENECA LIMITED , MELOEN, Robbert, Hans , PUIJK, Wouter, Cornelis , SCHAAPER, Wilhelmus, Martinus, Maria , SIJTSMA, Lolke , VAN AMERONGEN, Aart , BROEKAERT, Willem, Frans , DE SAMBLANX, Genoveva, Wivina , FANT, Franky , BORREMANS, Frans, Alois, Melania , REES, Sarah, Bronwen , VAN GELDER, Wilhelmus, Martinus, Josef
发明人： ZENECA LIMITED
IPC分类号： C12N15/29
CPC分类号： C12N15/8282 , A01N65/08 , A61K38/00 , C07K14/415
摘要： Antifungal peptides which comprise at least six amino acid residues identical to a run of amino acid residues found between position 21 and position 51 of the Rs-AFP2 antifungal protein sequence or of substantially homologous protein sequences. The peptides are useful for combating fungal diseases in agricultural, pharmaceutical or preservative applications.
摘要翻译：包含与Rs-AFP2抗真菌蛋白质序列的位置21和位置51之间发现的氨基酸残基相同的至少6个氨基酸残基或基本上同源的蛋白质序列的抗真菌肽。这些肽可用于防治农业，药物或防腐应用中的真菌疾病。

5. 发明申请

WO1994011511A1 BIOCIDAL CHITIN BINDING PROTEINS 审中-公开
标题翻译：生物化学结合蛋白
公开(公告)号：WO1994011511A1
公开(公告)日：1994-05-26
申请号：PCT/GB1993002179
申请日：1993-10-22
申请人： ZENECA LIMITED , BROEKAERT, Willem, Frans , CAMMUE, Bruno, Philippe, Angelo , OSBORN, Rupert, William , REES, Sarah, Bronwen
发明人： ZENECA LIMITED
IPC分类号： C12N15/29
CPC分类号： C07K14/415 , A01N65/20 , A01N65/32 , A01N65/38 , A01N65/44 , C12N15/8281 , C12N15/8282
摘要： Biocidal proteins capable of isolation from seeds have been characterised. The proteins have an amino acid sequence containing the common cysteine/glycine domain of Chitin-binding Plant Proteins but show substantially better activity against pathogenic fungi, a higher ratio of basic amino acids to acidic amino acids, and/or antifungal activity which results in increased hyphal branching. Antimicrobial proteins isolated from Capsicum, Briza and related species are provided. The proteins show a wide range of antifungal activity and are active against Gram-positive bacteria. DNA encoding the proteins may be isolated and incorporated into vectors. Plants may be transformed with this DNA. The proteins find agricultural or pharmaceutical application as antifungal or antibacterial agents. Transgenic plants expressing the protein will show increased disease resistance.
摘要翻译：已经表征了能够从种子中分离的杀生物蛋白质。蛋白质具有含有几丁质结合植物蛋白质的共同半胱氨酸/甘氨酸结构域的氨基酸序列，但对病原真菌显示出更好的活性，碱性氨基酸与酸性氨基酸的比例更高，和/或抗真菌活性增加菌丝分枝提供从辣椒，Briza和相关物种分离的抗菌蛋白质。蛋白质显示出广泛的抗真菌活性，并且对革兰氏阳性菌具有活性。编码蛋白质的DNA可以被分离并并入载体中。可以用这种DNA转化植物。蛋白质发现农业或药物应用作为抗真菌剂或抗菌剂。表达蛋白质的转基因植物将显示出增加的抗病性。

6. 发明申请

WO1995018229A1 ANTIMICROBIAL PROTEINS 审中-公开
标题翻译：抗微生物蛋白
公开(公告)号：WO1995018229A1
公开(公告)日：1995-07-06
申请号：PCT/GB1994002766
申请日：1994-12-19
申请人： ZENECA LIMITED , BROEKAERT, Willem, Frans , CAMMUE, Bruno, Philippe, Angelo , OSBORN, Rupert, William , REES, Sarah, Bronwen
发明人： ZENECA LIMITED
IPC分类号： C12N15/29
CPC分类号： C12N15/8282 , A01N65/08 , C07K14/415 , C12N15/8281
摘要： Antimicrobial proteins capable of isolation from seeds of Heuchera or Aesculus show a wide range of antifungal activity and some activity against Gram-positive bacteria. DNA encoding the proteins may be isolated and incorporated into vectors. Plants transformed with this DNA may be produced. The proteins find commercial application as antifungal or antibacterial agents; transformed plants will show increased disease-resistance.
摘要翻译：能够分离自Hecehera或Eesculus的种子的抗微生物蛋白质显示出广泛的抗真菌活性和对革兰氏阳性菌的一些活性。编码蛋白质的DNA可以被分离并并入载体中。可以生产用该DNA转化的植物。蛋白质作为抗真菌剂或抗菌剂找到商业应用; 转化的植物将显示增加的抗病性。

7. 发明申请

WO1994016076A1 ANTIMICROBIAL-PROTEIN-PRODUCING ENDOSYMBIOTIC MICROORGANISMS 审中-公开
标题翻译：抗微生物蛋白生产内生微生物
公开(公告)号：WO1994016076A1
公开(公告)日：1994-07-21
申请号：PCT/GB1994000012
申请日：1994-01-05
申请人： ZENECA LIMITED , DUBOCK, Adrian, Christopher , POWELL, Keith, Adrian , REES, Sarah, Bronwen
发明人： ZENECA LIMITED
IPC分类号： C12N15/29
CPC分类号： C07K14/415 , A01N63/00 , C12N15/74
摘要： There is provided a method of producing an antimicrobial-protein-producing microorganism capable of entering into an endosymbiotic relationship with a plant host comprising the combination of genetic material encoding a plant-derived antimicrobial protein with an endophyte. Examples of potent plant-derived antimicrobial proteins are given. A method for protecting a plant host from disease comprising treating the plant host with the antimicrobial-protein-producing microorganism is described.
摘要翻译：提供了一种能够与植物宿主进行内共生关系的生产抗微生物蛋白质的微生物的方法，所述植物宿主包含编码植物来源的抗微生物蛋白质的遗传物质与内生真菌的组合。给出有效的植物来源的抗微生物蛋白质的实例。描述了用于保护植物宿主免受疾病的方法，包括用产生抗微生物蛋白的微生物处理植物宿主。

8. 发明申请

WO1998000023A2 PLANT PROTECTION METHOD 审中-公开
标题翻译：植物保护方法
公开(公告)号：WO1998000023A2
公开(公告)日：1998-01-08
申请号：PCT/GB1997001672
申请日：1997-06-20
申请人： ZENECA LIMITED , BROEKAERT, Willem, Frans , THOMMA, Bart, Pierre, Helene, Joseph , PENNINCKX, Iris, Anne, Marie, Armande , TERRAS, Franky, Raymond, Gerard , MANNERS, John, Michael , KAZAN, Kemal
发明人： ZENECA LIMITED
IPC分类号： A01N61/00
CPC分类号： A01N37/42 , A01N27/00 , A01N61/00 , C07K14/415 , C12N15/8237 , C12N15/8279
摘要： A method of protecting a plant against a pathogen is described, the method comprising inducing expression of a plant defensin gene by stimulating the jasmonate and/or ethylene pathways. Also described is a method of inducing expression of a plant defensin gene, a composition which is capable of inducing expression of a plant defensin gene and a method for screening compounds giving resistance-inducing activity. Preferably, the pathogen is a necrotrophic pathogen.
摘要翻译：描述了保护植物免受病原体的方法，所述方法包括通过刺激茉莉酮酸和/或乙烯途径诱导植物防御素基因的表达。还描述了诱导植物防御素基因表达的方法，能够诱导植物防御素基因表达的组合物和筛选产生抗性诱导活性的化合物的方法。优选地，病原体是坏死性病原体。

9. 发明申请

WO1998029507A1 PLASTISOL COMPOSITIONS 审中-公开
标题翻译： PLASTISOL组合物
公开(公告)号：WO1998029507A1
公开(公告)日：1998-07-09
申请号：PCT/GB1996003246
申请日：1996-12-31
申请人： ZENECA LIMITED , OVEREND, Andrew, Stuart , PADGET, John, Christopher , COOPER, David, Henry
发明人： ZENECA LIMITED
IPC分类号： C08L33/06
CPC分类号： C08L33/06 , B29C2793/0081 , B29D2030/1685 , C09D133/06 , C08L2666/02 , C08L2666/28
摘要： Acrylic plastisol composition comprising a plasticiser component, a plasticiser compatible acrylic polymer powder component of primary particle size >/=0.4 mu m and preferably not more than 30 mu m, and Tg >/=65 DEG C, and a plasticiser incompatible polymer powder component, where both polymers are preformed, are present in the weight ratio 95:5 to 40:60, and in combination are compatibly formulated with the plasticiser component.
摘要翻译：丙烯酸增塑剂组合物，其包含增塑剂组分，一次粒径≥0.4μm，优选不大于30μm，Tg> = 65℃的增塑剂相容的丙烯酸类聚合物粉末组分和增塑剂不相容的聚合物粉末组分，其中两种聚合物都被预先形成，其重量比为95:5至40:60，并且与增塑剂组分配合使用。

10. 发明申请

WO1998028306A1 PROCESS FOR THE PREPARATION OF AN ORGANOZINC REAGENT 审中-公开
标题翻译：制备有机原料试剂的方法
公开(公告)号：WO1998028306A1
公开(公告)日：1998-07-02
申请号：PCT/GB1997003443
申请日：1997-12-15
申请人： ZENECA LIMITED , BLACKER, Andrew, John , FIELDEN, Jan, Michael
发明人： ZENECA LIMITED
IPC分类号： C07F03/06
CPC分类号： C07D213/30 , C07B53/00 , C07B2200/07 , C07C29/40 , C07F3/06 , C07C33/22 , C07C33/46 , C07C33/20
摘要： A process for the preparation of an aromatic organozinc compound is provided. The process comprises reacting a zinc chloride, bromide or iodide and an organometallic compoud of another metal comprising an aromatic moiety, producing a reaction product comprising an organozinc compound and a halide salt of the other metal. The reaction product is then contacted with a liquid hydrocarbon, in which the organozinc compound is soluble and the halide salt of the other metal is of low solubility. The halide salt of the other metal is separated from the hydrocarbon, and the organozinc compound can be recovered from the hydrocarbon. Organozinc compounds produced by this process can be employed to generate chiral centres in suitable substrates.
摘要翻译：提供了一种制备芳香族有机锌化合物的方法。该方法包括使氯化锌，溴化物或碘化物与包含芳族部分的另一种金属的有机金属化合物反应，产生包含有机锌化合物和另一种金属的卤化物盐的反应产物。然后使反应产物与液态烃接触，其中有机锌化合物是可溶的，另一种金属的卤化物盐的溶解度低。另一种金属的卤化物盐与烃分离，有机锌化合物可以从烃中回收。通过该方法制备的有机锌化合物可用于在合适的底物中产生手性中心。

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