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1. 发明申请

WO1997017457A2 STABLE PACKAGING CELL LINE PRODUCING PSEUDOTYPED RETROVIRUSES 审中-公开
标题翻译：稳定的包装细胞系生产PSEUDOTYPED RETROVIRUSUS
公开(公告)号：WO1997017457A2
公开(公告)日：1997-05-15
申请号：PCT/US1996017807
申请日：1996-11-07
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , WASHINGTON UNIVERSITY , ORY, Daniel, S. , SADELAIN, Michel , MULLIGAN, Richard, C. , SCHAFFER, Jean, E.
发明人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , WASHINGTON UNIVERSITY
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , C12N2740/13052
摘要： The present invention relates to a stable, pseudotyped retrovirus packaging cell line comprising packaging cells which generate helper-free recombinant pseudotyped retrovirus. The packaging cell line comprises one or more non-retroviral expression constructs, such as an expression construct with the human cytomegalovirus (CMV) immediate early promoter or derivatives of this promoter (e.g., pMD), which direct expression of: (a) retroviral gagpol genes and (b) a non-retroviral gene which is under the control of an inducible operator system and whose gene product pseudotypes retroviral core virions. The present invention further relates to a method of making a stable, pseudotyped retrovirus packaging cell line which generates helper-free recombinant pseudotyped retrovirus. The present invention further relates to the particular packaging cell lines described herein (i.e., H29 gagpol, H29 new gagpol) and the particular cells and constructs (i.e., packaging elements) used to produce the stable, pseudotyped retrovirus packaging cell line described herein (e.g., H29 cells and pMD, pMDtet, pMDtet.G, PMD.gagpol, pMD.new gagpol constructs). The present invention relates to a retroviral vector for producing a cDNA library for expression in mammalian cells, comprising two retroviral long terminal repeats, a cloning site for insertion of cDNA and a cytomegalovirus promoter. The invention also relates to a cDNA library for expression in mammalian cells, the library comprising retroviral vectors of the present invention. The present invention also relates to a method of expression cloning in mammalian cells. The present invention also relates to a method of cDNA expression cloning in mammalian cells. The present invention also relates to a method of identifying a gene defect responsible for a mutant phenotype using cDNA expression cloning by complementation in mammalian cells.
摘要翻译：本发明涉及稳定的假型逆转录病毒包装细胞系，其包含产生无辅助重组假型逆转录病毒的细胞。包装细胞系包含一个或多个非逆转录病毒表达构建体，例如具有人巨细胞病毒（CMV）立即早期启动子或该启动子的衍生物（例如，pMD）的表达构建体，其直接表达：（a）逆转录病毒gagpol 基因和（b）非诱导型操纵子系统控制下的非逆转录病毒基因，其基因产物假型为逆转录病毒核心病毒粒子。本发明还涉及制备稳定的假型逆转录病毒包装细胞系的方法，该细胞系产生无辅助重组假型逆转录病毒。本发明还涉及本文所述的特定包装细胞系（即，H29加格尔波尔，H29新加格尔）以及用于产生本文所述的稳定的假型逆转录病毒包装细胞系的特定细胞和构建体（即包装元件）（例如，，H29细胞和pMD，pMDtet，pMDtet.G，PMD.gagpol，pMD.新的gagpol构建体）。本发明涉及用于产生用于在哺乳动物细胞中表达的cDNA文库的逆转录病毒载体，其包含两个逆转录病毒长末端重复序列，用于插入cDNA的克隆位点和巨细胞病毒启动子。本发明还涉及用于在哺乳动物细胞中表达的cDNA文库，该文库包含本发明的逆转录病毒载体。本发明还涉及在哺乳动物细胞中表达克隆的方法。本发明还涉及哺乳动物细胞中cDNA表达克隆的方法。本发明还涉及使用哺乳动物细胞中互补的cDNA表达克隆来鉴定负责突变体表型的基因缺陷的方法。

2. 发明申请

WO1996034879A1 IDENTIFICATION OF ENANTIOMERIC LIGANDS 审中-公开
标题翻译：识别通配符号
公开(公告)号：WO1996034879A1
公开(公告)日：1996-11-07
申请号：PCT/US1996006155
申请日：1996-05-02
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , SCHUMACHER, Antonius, Nicolaas, Maria , KIM, Peter, S.
发明人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
IPC分类号： C07K01/04
CPC分类号： C40B30/04 , C07H21/00 , C07K1/047 , C07K14/001 , C12N15/1034 , G01N33/6845
摘要： A method of identifying macromolecules (peptides, olignucleotides, sugars and macromolecular complexes, such as RNA-protein complexes, protein-lipid complexes), which are not of the natural handedness (not of the chirality as they occur in nature or as a wild type molecule) and which are lignds for other chiral macromolecules.
摘要翻译：鉴定大分子（肽，寡核苷酸，糖和大分子复合物，如RNA-蛋白复合物，蛋白质 - 脂质复合物）的方法，其不是天然的手性（不是手性，它们在自然界或野生型中发生）分子），它们是其他手性大分子的木质素。

3. 发明申请

WO1994016109A1 MEMBRANE FUSION EVENTS AND MEANS FOR ALTERING SAME 审中-公开
标题翻译：膜融合事件及其改进方法
公开(公告)号：WO1994016109A1
公开(公告)日：1994-07-21
申请号：PCT/US1994000709
申请日：1994-01-18
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
发明人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , CARR, Chavela, M. , KIM, Peter, S.
IPC分类号： C12Q01/70
CPC分类号： C07K14/005 , A61K45/00 , C12N2760/16022 , C12Q1/18
摘要： The present invention relates to a method of altering (reducing or enhancing) membrane fusion events in a wide variety of contexts, including infection of cells by organisms (such as pathogens including viruses and particularly influenza virus), cell-cell interactions or fusions (such as cell signalling and sperm-ovum union) and intercellular membrane fusion events. It further relates to agents useful in the method of altering membrane fusion events and to a method of identifying or designing agents which alter membrane fusion events.
摘要翻译：本发明涉及一种在多种情况下改变（减少或增强）膜融合事件的方法，包括由有机体（例如包括病毒，特别是流感病毒在内的病原体）感染细胞，细胞 - 细胞相互作用或融合（如作为细胞信号传导和精子 - 卵子联合）和细胞间膜融合事件。它还涉及可用于改变膜融合事件的方法的试剂以及鉴定或设计改变膜融合事件的试剂的方法。

4. 发明申请

WO1992012242A1 MODIFIED HEPATOCYTES AND USES THEREFOR 审中-公开
标题翻译：改良的肝细胞及其用途
公开(公告)号：WO1992012242A1
公开(公告)日：1992-07-23
申请号：PCT/US1991009700
申请日：1991-12-23
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , HOWARD HUGHES MEDICAL CENTER
发明人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , HOWARD HUGHES MEDICAL CENTER , MULLIGAN, Richard, C. , WILSON, James, M.
IPC分类号： C12N15/00
CPC分类号： C12N15/86 , A61K38/00 , A61K48/00 , C07K14/635 , C07K14/705 , C12N5/067 , C12N2740/13043
摘要： Genetically engineered or transduced hepatocytes which express genetic material of interest introduced or incorporated into them, as well as methods of producing, transplanting and using the genetically engineered hepatocytes. The genetic material of interest can be incorporated through use of a vector, such as a recombinant retrovirus, which contains the genetic material of interest, or by other means.
摘要翻译：表达遗传材料的转基因或转导肝细胞引入或并入其中，以及生产，移植和使用遗传工程化肝细胞的方法。感兴趣的遗传物质可以通过使用载体，如包含感兴趣的遗传物质的重组逆转录病毒载体，或通过其他方式加入。

5. 发明申请

WO1988005823A2 MYCOBACTERIUM TUBERCULOSIS GENES ENCODING PROTEIN ANTIGENS 审中-公开
标题翻译： MYCOBACTERIUM TUBERCULOSIS GENES编码蛋白抗原
公开(公告)号：WO1988005823A2
公开(公告)日：1988-08-11
申请号：PCT/US1988000281
申请日：1988-02-01
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
发明人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , HUSSON, Robert, N. , YOUNG, Richard, A. , SHINNICK, Thomas, M.
IPC分类号： C12N15/00
CPC分类号： C07K14/35 , A61K39/00
摘要： Mycobacterium tuberculosis genes encoding five immunologically relevant proteins have been isolated by systematically screening a lambda gt11 recombinant DNA expression library with a collection of murine monoclonal antibodies directed against protein antigens of this pathogen. One of the M. tuberculosis antigens, a 65kD protein, has been shown to have determinants common to M. tuberculosis and M. leprae. In addition, genes encoding proteins of other mycobacteria (M. africanum, M. smegmatis, M. bovis BCG and M. avium) have been isolated. Isolation and characterization of genes encoding major protein antigens of M. tuberculosis make it possible to develop reagents useful in the diagnosis, prevention and treatment of tuberculosis. They can be used, for example, in the development of skin tests, serodiagnostic tests and vaccines specific for tuberculosis.
摘要翻译：已经通过用针对该病原体的蛋白质抗原的鼠单克隆抗体的集合系统地筛选λgt11重组DNA表达文库来分离编码五种免疫相关蛋白的结核分枝杆菌基因。结核分枝杆菌抗原（一种65kD蛋白）中的一种已被证明具有与结核分枝杆菌和麻疯病菌相似的决定因素。此外，已经分离了编码其他分枝杆菌蛋白质的基因（M. africanum，M.耻垢分枝杆菌，牛分枝杆菌BCG和鸟分枝杆菌）。编码结核分枝杆菌主要蛋白质抗原的基因的分离和表征使得开发可用于诊断，预防和治疗结核病的试剂成为可能。它们可以用于例如皮肤试验，血清诊断试验和特异性结核病疫苗的开发。

6. 发明申请

WO1987000201A1 EPITHELIAL CELLS EXPRESSING FOREIGN GENETIC MATERIAL 审中-公开
标题翻译：上皮细胞表达外源遗传材料
公开(公告)号：WO1987000201A1
公开(公告)日：1987-01-15
申请号：PCT/US1986001378
申请日：1986-06-30
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
发明人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , MORGAN, Jeffrey, R. , MULLIGAN, Richard, C.
IPC分类号： C12N15/00
CPC分类号： C07K14/61 , A61K35/36 , A61K38/00 , A61L27/54 , A61L27/60 , A61L2300/254 , A61L2300/258 , A61L2300/43 , A61L2300/64 , C07K14/635 , C12N15/85 , C12N15/86 , C12N15/90 , C12N2740/13041 , C12N2740/13043 , Y10S435/948
摘要： The foreign genetic material can be DNA or RNA which does not occur in epithelial cells; DNA or RNA which occurs in epithelial cells but is not expressed in them at levels which are biologically significant; DNA or RNA which occurs in epithelial and has been modified so that it is expressed in epithelial cells; and any DNA or RNA which can be modified to be expressed in epithelial cells, alone or in any combination thereof. In addition, epithelial cells can express genetic material encoding a selectable marker by which cells expressing the foreign genetic material can be expressed.
摘要翻译：外来遗传物质可以是不发生在上皮细胞中的DNA或RNA; 发生在上皮细胞中但不具有生物学意义的水平的DNA或RNA; DNA或RNA，其在上皮中发生并被修饰，使其在上皮细胞中表达; 以及可以修饰以在上皮细胞中单独或以任何组合表达的任何DNA或RNA。此外，上皮细胞可以表达编码可以表达外源遗传物质的细胞的选择标记的遗传物质。

7. 发明申请

WO1996009379A1 DNA PURIFICATION AND ISOLATION USING A SOLID PHASE 审中-公开
标题翻译：使用固相的DNA纯化和分离
公开(公告)号：WO1996009379A1
公开(公告)日：1996-03-28
申请号：PCT/US1995011839
申请日：1995-09-19
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
发明人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , HAWKINS, Trevor
IPC分类号： C12N15/10
CPC分类号： C12N15/1013 , Y10S435/975
摘要： A method of separating polynucleotides, such as DNA, RNA and PNA, from a solution containing polynucleotides by reversibly and non-specifically binding the polynucleotides to a solid surface, such as a magnetic microparticle, having a functional group-coated surface is disclosed. The salt and polyalkylene glycol concentration of the solution is adjusted to levels which result in polynucleotide binding to the magnetic microparticles. The magnetic microparticles with bound polynucleotides are separated from the solution and the polynucleotides are eluted from the magnetic microparticles.
摘要翻译：公开了一种通过将多核苷酸可逆地和非特异性地结合到具有官能团涂覆表面的固体表面的磁性微粒从包含多核苷酸的溶液中分离多核苷酸的方法，例如DNA，RNA和PNA。将溶液的盐和聚亚烷基二醇浓度调节至导致多核苷酸与磁性微粒结合的水平。从溶液中分离具有结合多核苷酸的磁性微粒，并从磁性微粒中洗脱多核苷酸。

8. 发明申请

WO1995003417A2 HOMOLOGOUSLY RECOMBINANT SLOW GROWING MYCOBACTERIA AND USES THEREFOR 审中-公开
标题翻译：非常重要的重组慢性生长因子及其用途
公开(公告)号：WO1995003417A2
公开(公告)日：1995-02-02
申请号：PCT/US1994008267
申请日：1994-07-22
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
发明人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , ALDOVINI, Anna , YOUNG, Richard, A.
IPC分类号： C12N15/74
CPC分类号： A61K39/04 , A61K38/00 , A61K39/00 , C07K14/005 , C07K14/35 , C07K14/52 , C07K14/55 , C12N9/88 , C12N13/00 , C12N15/74 , C12N15/87 , C12N2740/16022 , C12R1/32 , Y02A50/412
摘要： A method of transforming slow-growing mycobacteria, such as M. bovis BCG, M. leprae, M. tuberculosis, M. avium, M. intracellulare and M. africanum; a method of manipulating genomic DNA of slow-growing mycobacteria through homologous recombination; a method of producing homologously recombinant (HR) slow-growing mycobacteria in which heterologous DNA is integrated into the genomic DNA at a homologous locus; homologously recombinant (HR) slow-growing mycobacteria having heterologous DNA integrated into their genomic DNA at a homologous locus; and mycobacteria DNA useful as a genetic marker.
摘要翻译：转化缓慢生长的分枝杆菌的方法，例如牛分枝杆菌BCG，麻疯病，结核分枝杆菌，鸟分枝杆菌，胞内分枝杆菌和非洲雄蕊; 通过同源重组操纵慢生长分枝杆菌的基因组DNA的方法; 产生同源重组（HR）慢生长分枝杆菌的方法，其中异源DNA在同源基因座整合到基因组DNA中; 同源重组（HR）缓慢生长的分枝杆菌在同源基因座处整合到其基因组DNA中的异源DNA; 和分枝杆菌DNA可用作遗传标记。

9. 发明申请

WO1993020201A1 GAP-ASSOCIATED PROTEIN p190 AND TRANSDUCTION 审中-公开
标题翻译： GAP相关蛋白p190和转录
公开(公告)号：WO1993020201A1
公开(公告)日：1993-10-14
申请号：PCT/US1993003076
申请日：1993-03-31
申请人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
发明人： WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , SETTLEMAN, Jeffrey, E. , WEINBERG, Robert, A.
IPC分类号： C12N15/12
CPC分类号： C07K14/82 , A61K38/00 , C07K14/47
摘要： DNA which encodes p190 protein of mammalian origin, the encoded p190 protein, antibodies specific for the encoded p190 protein, a method of altering formation of a complex between p190 protein and the r &cir& _a &cir& _s &cir& _ GTPase activating protein (GAP), agents useful in the method and a method of altering transduction of messages or signals from p21 to the nucleus, are disclosed.
摘要翻译：编码哺乳动物来源的p190蛋白质的DNA，编码的p190蛋白质，编码的p190蛋白质特异的抗体，改变p190蛋白质和r＆cir＆amp; c＆amp; GTPase激活蛋白（GAP），代谢物之间复合物形成的方法公开了用于将消息或信号从p21 向核转换的方法和方法。

10. 发明申请

WO1991013172A1 TRANSCRIPTION FACTORS HAVING A PATHOGENETIC ROLE IN HUMAN NEOPLASIAS 审中-公开
标题翻译：在人类神经系统中具有致病作用的转录因子
公开(公告)号：WO1991013172A1
公开(公告)日：1991-09-05
申请号：PCT/US1991001168
申请日：1991-02-22
申请人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD, JR. , WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH
发明人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD, JR. , WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , CLEARY, Michael, L. , MELLENTIN, Julia, D. , BALTIMORE, David , MURRE, Cornelis , McCAW, Patrick, S.
IPC分类号： C12Q01/68
CPC分类号： C07K16/3061 , C12Q1/6886 , C12Q2600/156 , C12Q2600/158
摘要： A method of detecting and/or quantitating either or both of the genes localized to the breakpoint of a consistently recurring chromosomal translocation present in a human neoplasm, the fusion DNA which crosses the breakpoint, or their encoded products, as well as reagents useful in the method. In particular, a method of detecting either or both of the genes localized to the t(1;19) breakpoint on chromosome 19 and chromosome 1 in human acute lymphoblastic leukemias, the fusion DNA which crosses the breakpoint or their encoded products.
摘要翻译：检测和/或定量存在于人肿瘤中存在的一致性重复的染色体易位的断点中的一种或两种的基因或跨过断裂点的融合DNA或其编码产物的方法，以及用于方法。特别地，一种检测定位于人类急性淋巴细胞白血病中的染色体19和染色体1上的t（1; 19）断点上的任一个或两个基因的方法，穿过断点的融合DNA或其编码产物。

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