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    • 6. 发明申请
    • PROCESSES AND MATERIALS FOR CARRYING OUT SPECIFIC BINDING ASSAYS
    • 执行特定绑定测试的过程和材料
    • WO1986001899A1
    • 1986-03-27
    • PCT/GB1985000428
    • 1985-09-16
    • UNILEVER PLCUNILEVER NVDAVIS, Paul, JamesBOSLEY, John, Anthony
    • UNILEVER PLCUNILEVER NV
    • G01N33/53
    • G01N33/581G01N33/53G01N33/54306G01N33/582G01N33/583
    • A process for carrying out a label-linked specific binding assay, e.g. an immunoassay, comprising the steps of: providing in an aqueous reaction zone a member of a specific binding pair to participate in the assay, linked to a label which either is a first coreagent which is reactive with a second coreagent in a reaction to produce a detectable assay result, or is a generator of said coreagent; providing a dispersed quantity of said second coreagent in said reaction, or of a generator of said coreagent, within a discretely bounded zone adjacent to said aqueous reaction zone and formed by a zone-forming carrier material, which also carries in immobilised form a member of said specific binding pair; providing in said aqueous reaction zone a consumer of one of said coreagents in said reaction, thereby to ensure the substantial absence of said coreagent in said aqueous reaction zone; wherein said discretely bounded zone comprises a barrier sufficient to prevent entry of the consumer reagent from said aqueous reaction zone into the bounded zone, thereby to separate the consumer reagent from the dispersed quantity of second coreagent or generator thereof in the bounded zone; and allowing the specific binding reactions to take place whereby the assay reaction results in the binding of a variable quantity, dependent on the quantity of labelled member of said specific binding pair directly or indirectly to said immobilised specific binding material, so that said detectable assay result is produced by reaction of bound label or said coreagent generated by said bound label with said coreagent in said bounded zone.
    • 8. 发明申请
    • MONITORING METHOD
    • 监测方法
    • WO1994004926A1
    • 1994-03-03
    • PCT/EP1993002148
    • 1993-08-10
    • UNILEVER PLCUNILEVER NVUNIPATH LIMITEDCATT, MichaelCOLEY, JohnDAVIS, Paul, James
    • UNILEVER PLCUNILEVER NVUNIPATH LIMITED
    • G01N33/74
    • G01N33/689A61B10/0012G01N33/743G01N33/76G01N2800/36
    • A method of monitoring the status of a current ovulation cycle of an individual human female subject, involving testing of the body fluid concentration of an analyte of significance in relation to the status of the ovulation cycle, such as urinary E3G, during at least part of the pre-ovulation phase of the current ovulation cycle of the individual subject, and identification from the results of such testing of an analyte concentration change indicative of imminent ovulation, relative to an analyte concentration reference value that has been adapted to the individual human subject on the basis of analyte concentration test data obtained from the individual human subject during one or more previous ovulation cycles. Preferably testing for said analyte concentration during the current ovulation cycle is commenced a plurality of days (preferably at least 5) following the onset of menses but at least 2 numerical days in advance of the earliest numerical day on which actual ovulation has occurred in one or more previous ovulation cycles in the same individual subject.
    • 一种监测个体人类女性受试者当前排卵周期状态的方法,包括在至少部分时间内测量相对于排卵周期状态(例如尿E3G)的有意义的分析物的体液浓度 个体受试者的当前排卵周期的排卵前阶段,以及相对于已经适应于个体人受试者的分析物浓度参考值的来自对这种测试的分析物浓度变化的指示即将来临的排卵的结果的鉴定 在一个或多个以前的排卵周期中从个体人受试者获得的分析物浓度测试数据的基础。 优选地,在当前排卵周期期间对所述分析物浓度的测试在月经开始之后开始多天(优选至少5天),但是在实际排卵发生在一个或多个之前的最早数字日之前的至少2个数天 在同一个体科目中更多的排卵周期。