专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2013013304A1 METHODS AND DEVICES FOR ELECTRICAL SAMPLE PREPARATION 审中-公开
标题翻译：电子样品制备的方法和装置
公开(公告)号：WO2013013304A1
公开(公告)日：2013-01-31
申请号：PCT/CA2012/000698
申请日：2012-07-25
申请人： QVELLA CORPORATION , TALEBPOUR, Samad , KHINE, Aye Aye , MAASKANT, Robert , ALAVIE, Tino
发明人： TALEBPOUR, Samad , KHINE, Aye Aye , MAASKANT, Robert , ALAVIE, Tino
IPC分类号： C12M1/42 , B81B1/00 , B81B7/02 , C12M1/33 , C12N1/06 , C12N13/00 , C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/502715 , B01L3/502738 , B01L3/502753 , B01L7/52 , B01L2200/0631 , B01L2200/143 , B01L2300/0645 , B01L2300/0681 , B01L2300/0809 , B01L2300/0858 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/14 , B01L2300/1833 , B01L2400/0694 , C12M35/02 , C12M47/06 , C12N1/066 , C12N1/08 , C12N13/00 , C12Q1/686 , C12Q3/00
摘要： Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.
摘要翻译：提供了用于电裂解细胞并从细胞释放大分子的装置和方法。提供了一种微流体装置，其包括具有亚毫米级厚度的平面通道，并且在其上内表面和下内表面上包括电极。在用液体填充通道之后，使得通道包含液体内的单元，在通道电极之间施加一系列交替极性的电压脉冲，其中电压脉冲的幅度和电压脉冲的脉冲宽度对于引起细胞的不可逆电穿孔。该通道被配置为具有热性质，使得施加电压由于焦耳加热而导致快速升温，以从电镀单元释放大分子。通道还可以包括用于在电处理之前捕获和集中电池的内部滤波器。

2. 发明申请

WO2011014946A1 CELL CONCENTRATION, CAPTURE AND LYSIS DEVICES AND METHODS OF USE THEREOF 审中-公开
标题翻译：细胞浓度，捕获和检测装置及其使用方法
公开(公告)号：WO2011014946A1
公开(公告)日：2011-02-10
申请号：PCT/CA2010/001176
申请日：2010-07-30
申请人： TALEBPOUR, Samad , KHINE, Aye Aye , LEONARD, Stephen W. , MAASKANT, Robert , ALAVIE, Tino
发明人： TALEBPOUR, Samad , KHINE, Aye Aye , LEONARD, Stephen W. , MAASKANT, Robert , ALAVIE, Tino
IPC分类号： C12M1/42 , C12M1/33 , C12M1/34 , C12Q1/02 , C12Q1/68 , G01N27/26 , G01N33/50 , G01N33/543 , C12N11/00 , C12N13/00
CPC分类号： G01N33/5438 , B01L3/502715 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0645 , B01L2300/0809 , C12M23/16 , C12M25/00 , C12M25/04 , C12M29/10 , C12M35/02 , C12M41/38 , C12N1/066 , C12N11/14 , C12N13/00 , G01N33/5005
摘要： The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.
摘要翻译：本发明提供一种微流体装置及其用于浓缩和捕获细胞的方法。在层流下相对于样品施加脉冲非法拉第电场，这导致带电分析物的浓度和捕获。有利地，选择脉冲定时以避免与通道内的离子屏蔽相关的问题。通道内的至少一个电极涂有绝缘层，以防止法拉第电流在通道中流动。在脉冲施加单极电压给电极的情况下，样品内的带电分析物经由瞬时电泳力朝向其中一个电极移动。

3. 发明申请

WO2006094388A1 AUTOMATED ANALYZER 审中-公开
标题翻译：自动分析仪
公开(公告)号：WO2006094388A1
公开(公告)日：2006-09-14
申请号：PCT/CA2006/000321
申请日：2006-03-07
申请人： NOVX SYSTEMS INC. , ALAVIE, Tino , BECKLEY, Keith , HUDSON, Andrew , LEONARD, Stephen, W. , MAASKANT, Robert , TALEBPOUR, Samad , YANG, William
发明人： ALAVIE, Tino , BECKLEY, Keith , HUDSON, Andrew , LEONARD, Stephen, W. , MAASKANT, Robert , TALEBPOUR, Samad , YANG, William
IPC分类号： G01N35/02 , G01N21/76 , G01N21/62 , G01N35/10
CPC分类号： G01N35/0092 , G01N21/253 , G01N35/028 , G01N35/109 , G01N2001/288 , G01N2035/00326 , G01N2035/0422 , G01N2035/0465 , Y10T436/11 , Y10T436/111666 , Y10T436/113332 , Y10T436/2575
摘要： The present invention provides a bar-code driven, completely automated, microplate-based analyzer system for performing chemical, biochemical or biological assays. The analyzer is a modular, bench-top instrument that compactly integrates subsystems for sample dispensing, liquid handling, microplate transport, thermal incubation, vortexing, solid phase separation and optical reading. An internal processor is included for automating the instrument, and a user interface to facilitate communication with the operator via a touch-sensitive liquid-crystal display (LCD), and communicating with a remote network via multiple protocols. The analyzer includes firmware resident within the processing system and the user interface allows the operator to select pre-defined assay batch protocols and the user interface is configured in such as way so as to restrict an operator from programming the firmware.
摘要翻译：本发明提供了用于进行化学，生物化学或生物测定的条形码驱动的，完全自动化的基于微孔板的分析仪系统。分析仪是一种模块化的台式仪器，可紧凑集成样品分配，液体处理，微孔板输送，热孵育，涡旋，固相分离和光学读取的子系统。包括用于使仪器自动化的内部处理器，以及用于通过触敏液晶显示器（LCD）促进与操作者通信的用户接口，以及经由多个协议与远程网络通信。分析器包括驻留在处理系统内的固件，用户界面允许操作员选择预定义的测试批处理协议，并且用户界面被配置为以限制操作者编程固件的方式。

4. 发明申请

WO2007098576A1 MICROPLATE ASSAY KIT 审中-公开
标题翻译：麦克风测试包
公开(公告)号：WO2007098576A1
公开(公告)日：2007-09-07
申请号：PCT/CA2007/000293
申请日：2007-02-26
申请人： NOVX SYSTEMS INC. , TALEBPOUR, Samad , LEONARD, Stephen, W. , BECKLEY, Keith , MAASKANT, Robert
发明人： TALEBPOUR, Samad , LEONARD, Stephen, W. , BECKLEY, Keith , MAASKANT, Robert
IPC分类号： G01N35/00 , C12M1/34 , G01N21/03 , G01N33/50 , G01N35/10
CPC分类号： B01L3/50853 , B01L2200/148 , B01L2200/16 , B01L2300/044 , B01L2300/0829
摘要： The present invention provides a microplate-based assay kit that incorporates all assay reagents and standards in a simple and efficient format and may be used in biochemical assays. The assay kit includes pre-filled, pre-sealed and barcoded microplates with a standard physical footprint for use in a microplate-based automated analyzer system which enables simple and efficient operation by an unskilled user via per-use factory-sealed and barcoded reagent and calibrator microplates. Such microplates allow the user to run a broad test menu without the need to monitor the arrangement and supply of internally stored reagents and standards, thus greatly simplifying the user experience and eliminating the need for highly skilled users.
摘要翻译：本发明提供了一种基于微板的测定试剂盒，其以简单且有效的形式并入所有测定试剂和标准品，并可用于生物化学测定。测定试剂盒包括预填充的预密封和条形码微孔板，具有用于基于微孔板的自动分析仪系统的标准物理尺寸，使得非熟练使用者能够通过使用工厂密封和条形码的试剂进行简单有效的操作，校准器微孔板。这样的微板允许用户运行广泛的测试菜单，而不需要监视内部存储的试剂和标准的布置和供应，从而大大简化了用户体验并且消除了对高技能用户的需要。

5. 发明申请

WO2006081651A1 IMMUNOASSAYS WITH ENHANCED SELECTIVITY 审中-公开
标题翻译：具有增强选择性的免疫球蛋白
公开(公告)号：WO2006081651A1
公开(公告)日：2006-08-10
申请号：PCT/CA2006/000063
申请日：2006-01-19
申请人： NOVX SYSTEMS INC. , TALEBPOUR, Samad , LEONARD, Stephen, W. , EMADI-KONJIN, Pasha
发明人： TALEBPOUR, Samad , LEONARD, Stephen, W. , EMADI-KONJIN, Pasha
IPC分类号： G01N33/544 , G01N33/573
CPC分类号： G01N33/54393 , G01N33/5306 , G01N2600/00
摘要： The present invention provides a method for the detection of an analyte in a specimen comprising two steps. First, the specimen is contacted with a substrate having receptors bound thereon, having higher affinity for cross-reactive substances than for the analyte, in order to increase the ratio of the analyte to the cross-reactive substances. Then, the treated specimen is assayed by using another receptor while decreasing the effects of the cross-reactive substance contained in the original specimen. The receptors in the first step are preferably molecular imprinted polymers that are capable of absorbing multiple cross-reactive substances.
摘要翻译：本发明提供一种用于检测样品中分析物的方法，包括两个步骤。首先，将样品与具有结合于其上的受体的底物接触，对于交叉反应性物质的亲和性高于分析物，以增加分析物与交叉反应物质的比例。然后，通过使用另一种受体同时降低原始样品中包含的交叉反应物质的影响来测定处理的样品。第一步中的受体优选是能够吸收多个交叉反应物质的分子印迹聚合物。

6. 发明申请

WO2008109977A1 METHOD OF COMPENSATION OF DOSE-RESPONSE CURVE OF AN ASSAY FOR SENSITIVITY TO PERTURBING VARIABLES 审中-公开
标题翻译：补偿灵敏度测量变量的剂量响应曲线的方法
公开(公告)号：WO2008109977A1
公开(公告)日：2008-09-18
申请号：PCT/CA2007/000398
申请日：2007-03-12
申请人： NOVX SYSTEMS INC. , LEONARD, Stephen, W. , TALEBPOUR, Samad
发明人： LEONARD, Stephen, W. , TALEBPOUR, Samad
IPC分类号： G01N37/00 , G01N21/59 , G01N21/62 , G01N21/64 , G01N21/93
CPC分类号： G01N21/274 , G01N21/276 , G01N2201/1211
摘要： The present invention provides assays and methods of compensating for changes in the dose-response curve of an assay where such changes are due to variations in a perturbing variable such as, but not limited to temperature. This is achieved by a two-step method, the first step of which involves measurements of the dose-response curve, and thus the individual assay parameters, at many different values of the perturbing variable, spanning the expected range of the perturbing variable. In the second step, unknown samples are assayed simultaneously with a known standard at a chosen analyte concentration. During this measurement, the value of the perturbing variable is unknown and the dose-response curve is therefore also unknown. The different dose-response curves from the first step are used to determine a mathematical relationship between the assay parameters and the assay signal of the known standard. With this relationship, in which the value of the perturbing variable is implicit rather than explicit, assay parameters that are valid for the unknown value of the perturbing variable can be obtained by substituting the value of the assay signal from the known standard (measured when assaying the unknown samples) into the mathematical relationship and solving for the assay parameters. The method enables an accurate determination of the analyte concentration even when the perturbing variable is changing or fluctuating from one sample measurement to another. Once the first step is completed, the second step can be performed repeatedly to measure unknown samples with accuracy.
摘要翻译：本发明提供了用于补偿测定的剂量 - 反应曲线的变化的测定和方法，其中这些变化是由于扰动变量（例如但不限于温度）的变化引起的。这通过两步法实现，其第一步涉及剂量 - 响应曲线的测量，并且因此涉及扰动变量的许多不同值的单独测定参数，跨越扰动变量的预期范围。在第二步中，未知样品在选定的分析物浓度下与已知标准同时测定。在此测量期间，扰动变量的值是未知的，因此剂量 - 反应曲线也是未知的。使用来自第一步的不同剂量 - 响应曲线来确定测定参数与已知标准的测定信号之间的数学关系。通过这种关系，其中扰动变量的值是隐含的而不是显式的，可以通过用来自已知标准的测定信号的值（测定时测量）获得对扰动变量的未知值有效的测定参数未知样品）进入数学关系并解决测定参数。该方法即使当扰动变量从一个样品测量改变或波动到另一个样品测量时，也能够精确地确定分析物浓度。一旦完成第一步，可以重复执行第二步，准确测量未知样品。

7. 发明申请

WO2006094375A1 CORRECTION FOR TEMPERATURE DEPENDENCE OF ASSAYS 审中-公开
标题翻译：温度依赖性的校正
公开(公告)号：WO2006094375A1
公开(公告)日：2006-09-14
申请号：PCT/CA2006/000099
申请日：2006-01-26
申请人： NOVX SYSTEMS INC. , TALEBPOUR, Samad
发明人： TALEBPOUR, Samad
IPC分类号： G01N37/00 , G01N33/98 , G01N33/70 , G01N33/50
CPC分类号： G01N35/00693 , G01N2035/00702
摘要： The present invention provides a method to calibrate an assay for variations or fluctuations in an assay parameter such as, but not limited to, temperature that affect the measured assay signal and thereby to calculate an analyte concentration with improved accuracy. The method is preferably performed in a manner such that the measured signal from a single additional standard per batch is sufficient to provide the required calibration.
摘要翻译：本发明提供了一种用于校准化验参数中的变化或波动的方法，所述化验参数例如但不限于影响所测量的化验信号的温度，并由此通过改进来计算分析物浓度准确性。该方法优选以这样的方式进行，即每批来自单个附加标准的测量信号足以提供所需的校准。

8. 发明申请

WO2006092036A1 IMMUNOASSAY WITH EXTENDED DYNAMIC RANGE 审中-公开
标题翻译：免疫力超强动态范围
公开(公告)号：WO2006092036A1
公开(公告)日：2006-09-08
申请号：PCT/CA2006/000083
申请日：2006-01-23
申请人： NOVX SYSTEMS INC. , TALEBPOUR, Samad , LEONARD, Stephen, W.
发明人： TALEBPOUR, Samad , LEONARD, Stephen, W.
IPC分类号： G01N33/543
CPC分类号： G01N33/536
摘要： The present invention provides a method of performing a competitive assay for the detection and quantification of an analyte over an extended dynamic range. This is achieved by a multi-step sample addition method whereby different concentrations of sample are added at different times during the assay that produces a dose-response curve with multiple windows of detection. This multi-step sample addition method causes the dose-response curve of the composite assay to broaden, dramatically increasing the assay dynamic range.
摘要翻译：本发明提供了一种在扩展的动态范围内进行用于检测和定量分析物的竞争性测定的方法。这通过多步骤样品添加方法来实现，其中在测定期间在不同时间添加不同浓度的样品，其产生具有多个检测窗口的剂量 - 反应曲线。这种多步骤样品添加方法使得复合测定的剂量 - 反应曲线变宽，显着增加了测定动态范围。

9. 发明申请

WO2021258223A1 SYSTEMS AND METHODS FOR CLASSIFICATION OF MICROBIAL CELLS GROWN IN MICROCOLONIES 审中-公开
公开(公告)号：WO2021258223A1
公开(公告)日：2021-12-30
申请号：PCT/CA2021/050884
申请日：2021-06-25
申请人： QVELLA CORPORATION
发明人： TALEBPOUR, Samad , KHIMCHENKO, Anna , ASADISHEKARI, Maryam , LEONARD, Stephen Wesley
IPC分类号： C12Q1/04 , C12M1/00 , C12M1/34 , C12N1/00 , C12N1/24 , G02B21/36 , C12M41/36
摘要： Systems and methods are provided for classifying microbial cells according to morphological features of microcolonies. A dark-field objective is employed to acquire a dark-field image of a microcolony during a microcolony growth phase that is characterized by phenotypic expression of microcolony morphological features which evolve with time and are differentiated among classes of microbial cell types. The dark-field image is processed to classify the microcolony according to two or more microbial cell types, such as Gram status and/or speciation. The dark-field objective may have a numerical aperture selected to facilitate the imaging of microcolony morphological features, residing, for example, between 0.15 and 0.35. A set of dark-field images of a microcolony may be collected during the microcolony growth phase and processed to classify the microcolony. Classification may be performed according to a temporal ordering of the dark-field images, for example, using a recurrent neural network.

10. 发明申请

WO2015172255A1 APPARATUS, SYSTEM AND METHOD FOR PERFORMING AUTOMATED CENTRIFUGAL SEPARATION 审中-公开
标题翻译：用于执行自动离心分离的装置，系统和方法
公开(公告)号：WO2015172255A1
公开(公告)日：2015-11-19
申请号：PCT/CA2015/050449
申请日：2015-05-19
申请人： QVELLA CORPORATION
发明人： MAASKANT, Robert , YASOTHARAN, Sanjesh , TALEBPOUR, Samad , LEONARD, Stephen W. , ETEMAD-MOGHADAM, Cyrus , ZAHN, Alexandre
IPC分类号： B04B7/12 , B01D17/038 , B04B15/00 , F16K7/00
CPC分类号： B01L3/502753 , B01D17/0217 , B01D19/0052 , B01D21/262 , B01L3/5021 , B01L3/502715 , B01L3/502738 , B01L7/52 , B01L2200/027 , B01L2200/0684 , B01L2200/10 , B01L2300/0816 , B01L2300/0887 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2400/0409 , B01L2400/0487 , B01L2400/0655 , B04B5/0407 , B04B5/0421 , B04B13/00 , C12N15/1003 , F16K7/00 , F16K99/0015 , F16K2099/0084
摘要： Systems, methods and devices are provided for the automated centrifugal processing of samples. In some embodiments, an integrated fluidic processing cartridge is provided, in which a centrifugation chamber is fluidically interfaced, through a lateral surface thereof, with a microfluidic device, and wherein the integrated fluidic processing cartridge is configured to be inserted into a centrifuge for centrifugation. A cartridge interfacing assembly may be employed to interface with the integrated fluidic processing cartridge for performing various fluidic processing steps, such as controlling the flow of fluids into and out of the centrifugation chamber, and controlling the flow of fluids into the microfluidic device, and optionally for the further fluidic processing of fluids extracted to the microfluidic device. The integrated fluidic processing cartridge may include a supernatant chamber the extraction of a supernatant thereto, and a diluent chamber for diluting a suspension collected in the centrifugation chamber.
摘要翻译：提供了用于样品的自动离心处理的系统，方法和装置。在一些实施例中，提供了一种集成流体处理盒，其中离心室通过其侧表面与微流体装置流体连接，并且其中集成流体处理盒被配置为插入到离心机中用于离心。可以使用药筒接口组件与集成流体处理药筒接合，以执行各种流体处理步骤，例如控制流入和离开离心室的流动，以及控制流体进入微流体装置的流动，以及任选地用于进一步流体处理提取到微流体装置的流体。集成流体处理盒可以包括上清液的上清液，以及用于稀释收集在离心室中的悬浮液的稀释剂室。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式