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1. 发明申请

WO2007087336A2 METHODS FOR DETECTING NUCLEIC ACIDS USING MULTIPLE SIGNALS 审中-公开
标题翻译：使用多个信号检测核酸的方法
公开(公告)号：WO2007087336A2
公开(公告)日：2007-08-02
申请号：PCT/US2007/001859
申请日：2007-01-23
申请人： QUEST DIAGNOSTICS INVESTMENTS INCORPORATED , EXNER, Maurice , ROGERS, Amy
发明人： EXNER, Maurice , ROGERS, Amy
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6818 , C12Q2563/173 , C12Q2527/107
摘要： Disclosed is are methods for identifying a nucleic acid in a sample. In one example, the method includes: (a) contacting the nucleic acid in the sample with an oligonucleotide that is specific for the nucleic acid in the sample and that is labeled with at least a first fluorescent dye; (b) contacting the nucleic acid in the sample with a second fluorescent dye that is different from the first fluorescent dye, such that the second fluorescent dye interacts with the nucleic acid; (c) amplifying the nucleic acid if present in the sample; and (d) detecting the nucleic acid if present in the sample by observing fluorescence from the first fluorescent dye after the oligonucleotide hybridizes to the amplified nucleic acid and determining the melting temperature of the amplified nucleic acid by measuring the fluorescence of the second fluorescent dye. The second fluorescent dye may include a fluorescent intercalating agent.
摘要翻译：公开了用于鉴定样品中的核酸的方法。在一个实例中，该方法包括：（a）使样品中的核酸与样品中核酸特异的寡核苷酸接触，并用至少第一荧光染料标记; （b）使样品中的核酸与不同于第一荧光染料的第二荧光染料接触，使得第二荧光染料与核酸相互作用; （c）如果存在于样品中则扩增核酸; 和（d）在寡核苷酸与扩增的核酸杂交后，通过观察来自第一荧光染料的荧光检测样品中的核酸，并通过测量第二荧光染料的荧光来测定扩增的核酸的解链温度。第二荧光染料可以包括荧光插入剂。

2. 发明申请

WO2009018225A3 INTERNAL POSITIVE CONTROL FOR NUCLEIC ACID ASSAYS 审中-公开
公开(公告)号：WO2009018225A3
公开(公告)日：2009-02-05
申请号：PCT/US2008/071378
申请日：2008-07-28
申请人： QUEST DIAGNOSTICS INVESTMENTS INCORPORATED , ONG, Edgar , EXNER, Maurice , LEWINSKI, Michael A.
发明人： ONG, Edgar , EXNER, Maurice , LEWINSKI, Michael A.
IPC分类号： C12Q1/68
摘要： Compositions and methods for detecting a non-specific nucleic acid amplification inhibitor in a reaction are disclosed. An internal positive control (IPC) may be included in samples to be tested for target nucleic acids as a means to monitor non-specific inhibition of nucleic acid amplification and provide confidence in negative results obtained in target-specific assays. Provided herein are an IPC polynucleotide, IPC control primers, and IPC probes. Also provided are methods of using an IPC polynucleotide, primers, and probes to detect a non-specific nucleic acid amplification inhibitor.

3. 发明申请

WO2013006793A2 DIRECT AMPLIFICATION AND DETECTION OF VIRAL AND BACTERIAL PATHOGENS 审中-公开
标题翻译：直接放大和检测病毒和细菌病原体
公开(公告)号：WO2013006793A2
公开(公告)日：2013-01-10
申请号：PCT/US2012/045763
申请日：2012-07-06
申请人： QUEST DIAGNOSTICS INVESTMENTS INCORPORATED , EXNER, Maurice , JACKY, Luca , CHEN, Yin-Peng , MAI, Huong , CHEN, Jules , TABB, Michelle M. , AYE, Michael , ELEAZAR, Emberlee
发明人： EXNER, Maurice , JACKY, Luca , CHEN, Yin-Peng , MAI, Huong , CHEN, Jules , TABB, Michelle M. , AYE, Michael , ELEAZAR, Emberlee
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/701 , C12Q1/68 , C12Q1/6846 , C12Q1/686 , C12Q1/689 , C12Q1/70 , C12Q2527/101 , C12Q2527/125
摘要： Provided herein are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids.
摘要翻译：本文提供的方法是使用直接扩增从微生物鉴定目标核酸的存在或不存在，而没有提取核酸的步骤，但是保留测定提取的核酸的方法的基本相同的特异性和灵敏度。

4. 发明申请

WO2015042526A1 DETECTION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS IN BIOLOGICAL SAMPLES 审中-公开
标题翻译：在生物样品中检测耐甲氧西林黄连素
公开(公告)号：WO2015042526A1
公开(公告)日：2015-03-26
申请号：PCT/US2014/056777
申请日：2014-09-22
申请人： QUEST DIAGNOSTICS INVESTMENTS INCORPORATED , AYE, Michael
发明人： AYE, Michael , NAIR, Lakshmi , VINCENT, Heather , MAI, Huong , TABB, Michelle , EXNER, Maurice
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q2600/112 , C12Q2600/158 , C12Q2600/16 , G01N21/6428 , G01N2021/6432 , G01N2021/6441
摘要： Disclosed are methods of identifying a methicillin-resistant Staphylococcus aureus (MRSA) in a sample wherein the methods involve detecting a S. aureus-specific nucleic acid sequence, mecA and mecC, in the sample. Kits for determining the presence of MRSA in a sample are also provided.
摘要翻译：公开了在样品中鉴定耐甲氧西林金黄色葡萄球菌（MRSA）的方法，其中所述方法涉及检测样品中金黄色葡萄球菌特异性核酸序列mecA和mecC。还提供了用于确定样品中MRSA存在的试剂盒。

5. 发明申请

WO2012158997A1 SYSTEMS AND METHODS FOR DETECTING THE PRESENCE OF A SELECTED VOLUME OF MATERIAL IN A SAMPLE PROCESSING DEVICE 审中-公开
标题翻译：用于检测样品处理装置中选择的材料体积的存在的系统和方法
公开(公告)号：WO2012158997A1
公开(公告)日：2012-11-22
申请号：PCT/US2012/038498
申请日：2012-05-18
申请人： 3M INNOVATIVE PROPERTIES COMPANY , FOCUS DIAGNOSTICS, INC. , LUDOWISE, Peter D. , WHITMAN, David A. , ARMANTROUT, Kyle C. , EXNER, Maurice , JACKY, Lucien A.E. , TABB, Michelle
发明人： LUDOWISE, Peter D. , WHITMAN, David A. , ARMANTROUT, Kyle C. , EXNER, Maurice , JACKY, Lucien A.E. , TABB, Michelle
IPC分类号： B01L3/00
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502715 , B01L2200/0605 , B01L2300/087 , B01L2400/0409 , G01N21/6486 , G01N35/025 , G01N35/04 , G01N2035/0441
摘要： Systems and methods for processing sample processing devices. The system can include a sample processing device comprising a detection chamber, a motor configured to rotate the sample processing device about an axis of rotation, and an optical module operatively positioned relative to the sample processing device and configured to determine whether a selected volume of material is present in the detection chamber of the sample processing device. The method can include rotating the sample processing device about an axis of rotation, and determining whether a selected volume of material is present in the detection chamber, while rotating the sample processing device. In some embodiments, determining whether a selected volume of material is present can be performed by optically interrogating the detection chamber for an optical property of the material.
摘要翻译：用于处理样品处理装置的系统和方法。该系统可以包括样品处理装置，该样品处理装置包括检测室，被配置为围绕旋转轴线旋转样品处理装置的马达和相对于样品处理装置可操作地定位的光学模块，并配置成确定所选择的材料体积存在于样品处理装置的检测室中。该方法可以包括围绕旋转轴旋转样品处理装置，并且在旋转样品处理装置的同时确定所选择的材料体积是否存在于检测室中。在一些实施例中，确定所选择的材料体积是否存在可以通过光学询问该检测室以获得该材料的光学特性。

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