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1. 发明申请

WO2002078438A2 TISSUE-PREFERRED PROMOTER FROM MAIZE 审中-公开
标题翻译：组织优先促销者从MAIZE
公开(公告)号：WO2002078438A2
公开(公告)日：2002-10-10
申请号：PCT/US2002/009555
申请日：2002-03-28
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , NIU, Xiaomu , WANG, Haiyin , TOMES, Dwight, T. , HANTKE, Sabine
发明人： NIU, Xiaomu , WANG, Haiyin , TOMES, Dwight, T. , HANTKE, Sabine
IPC分类号： A01N
CPC分类号： C12N9/0032 , C07K14/415 , C12N15/8223 , C12N15/8295
摘要： The present invention provides a composition and method for regulating expresson of heterologous nucleotide sequences in a plant. The composition is novel nucleic acid sequence for a tissue-preferred promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译：本发明提供了调节植物中异源核苷酸序列表达的组合物和方法。该组合物是用于组织优选启动子的新型核酸序列。还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将植物细胞转化为含有与本发明的组织优选启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

2. 发明申请

WO2004090143A2 MODULATION OF CYTOKININ ACTIVITY IN PLANTS 审中-公开
标题翻译：植物中细胞因子活性的调节
公开(公告)号：WO2004090143A2
公开(公告)日：2004-10-21
申请号：PCT/US2004/010064
申请日：2004-04-02
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , HABBEN, Jeffrey, E. , ZINSELMEIER, Chris , TOMES, Dwight, T. , ABBITT, Shane, E. , HELENTJARIS, Timothy, G. , NIU, Xiaomu
发明人： HABBEN, Jeffrey, E. , ZINSELMEIER, Chris , TOMES, Dwight, T. , ABBITT, Shane, E. , HELENTJARIS, Timothy, G. , NIU, Xiaomu
IPC分类号： C12N15/82
CPC分类号： C12N9/0032 , C12N9/1085 , C12N15/8234 , C12N15/8261 , Y02A40/146
摘要： This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes that affect metabolically effective levels of cytokinins in plants, particularly in plant seeds and related female reproductive tissue. This invention further relates to transgenic plants having enhanced levels of cytokinin expression wherein the transgenic plant exhibits useful characteristics, such as improved seed size, decreased tip kernel abortion, increased seed set during unfavorable environmental conditions, or stability of yield. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for seed-preferred promoters known as eep1 and eep2 . A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译：本发明一般涉及植物分子生物学领域。更具体地说，本发明涉及影响植物中特别是植物种子和相关雌性生殖组织中细胞分裂素的代谢有效水平的基因的时间和/或空间调节表达的方法和试剂。本发明进一步涉及具有增强的细胞分裂素表达水平的转基因植物，其中转基因植物表现出有用的特征，例如改善的种子大小，降低的末梢核仁流产，在不利的环境条件下增加的种子集合或产量的稳定性。本发明还提供用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包含已知为eep1和eep2的种子优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

3. 发明申请

WO2005012516A2 MAIZE CELLULOSE SYNTHASES AND USES THEREOF 审中-公开
标题翻译：玉米纤维素合成及其用途
公开(公告)号：WO2005012516A2
公开(公告)日：2005-02-10
申请号：PCT/US2004/023640
申请日：2004-07-22
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , DHUGGA, Kanwarpal, S. , WANG, Haiyin , TOMES, Dwight, T. , HELENTJARIS, Timothy, G.
发明人： DHUGGA, Kanwarpal, S. , WANG, Haiyin , TOMES, Dwight, T. , HELENTJARIS, Timothy, G.
IPC分类号： C12N9/10
CPC分类号： C12N9/1059 , C07K14/415 , C12N15/8246 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.
摘要翻译：本发明提供分离的纤维素合酶核酸及其编码的蛋白质。本发明提供了关于改变植物中纤维素合成酶水平的方法和组合物。本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。

4. 发明申请

WO2011011273A1 THE USE OF DIMERIZATION DOMAIN COMPONENT STACKS TO MODULATE PLANT ARCHITECTURE 审中-公开
标题翻译：使用二维域组件堆栈来调整植物建筑
公开(公告)号：WO2011011273A1
公开(公告)日：2011-01-27
申请号：PCT/US2010/042231
申请日：2010-07-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , LAWIT, Shai, J. , TOMES, Dwight, T.
发明人： LAWIT, Shai, J. , TOMES, Dwight, T.
IPC分类号： C12N15/82 , C07K14/415 , A01H5/00
CPC分类号： C12N15/8261 , C07C31/08 , C07K14/415 , C12N15/8242 , C12N15/8255 , C12N15/8269 , C12N15/827 , C12N15/8293 , C12N15/8297 , C12P7/06 , Y02A40/146
摘要： This invention provides means for altering the harvest index of crop plants by modulating the expression of transgenic genes using dimerization domain and component stacks, thereby modulating plant architecture. The transgene/dimerization domain stacks are provided in a single transformation vector unit and are used to modulate plant growth, yield, and harvest index in plants.
摘要翻译：本发明提供了通过使用二聚化结构域和组分堆栈调节转基因基因的表达来改变作物植物的收获指数的方法，从而调节植物结构。转基因/二聚体结构域提供在单个转化载体单位中，用于调节植物的植物生长，产量和收获指数。

5. 发明申请

WO2011011273A9 THE USE OF DIMERIZATION DOMAIN COMPONENT STACKS TO MODULATE PLANT ARCHITECTURE 审中-公开
公开(公告)号：WO2011011273A9
公开(公告)日：2011-01-27
申请号：PCT/US2010/042231
申请日：2010-07-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , LAWIT, Shai, J. , TOMES, Dwight, T.
发明人： LAWIT, Shai, J. , TOMES, Dwight, T.
IPC分类号： C12N15/82 , C07K14/415 , A01H5/00
摘要： This invention provides means for altering the harvest index of crop plants by modulating the expression of transgenic genes using dimerization domain and component stacks, thereby modulating plant architecture. The transgene/dimerization domain stacks are provided in a single transformation vector unit and are used to modulate plant growth, yield, and harvest index in plants.

6. 发明申请

WO1998011200A1 APPARATUS FOR TISSUE PREPARATION 审中-公开
标题翻译：用于组织准备的装置
公开(公告)号：WO1998011200A1
公开(公告)日：1998-03-19
申请号：PCT/US1997016258
申请日：1997-09-12
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： PIONEER HI-BRED INTERNATIONAL, INC. , TOMES, Dwight, T. , WILLIAMS, John, G., K. , MOORE, Michael, E.
IPC分类号： C12M03/08
CPC分类号： C12M47/06 , G01N1/286 , G01N2001/2866 , G01N2001/2873
摘要： An apparatus (10) and method for preparing tissue samples for DNA, RNA or protein extraction of the present invention includes a reciprocating element (20) mounted to a frame (12) and connected to a plurality of tissue sample containers (52) containing tissue samples (62) and agitation members (60). By activating the reciprocating element (20), the tissue samples (62) are ground by the agitation members (60). A control circuit (18) is connected to the reciprocating element (20) for controlling the speed and duration of the agitation.
摘要翻译：用于制备本发明的DNA，RNA或蛋白质提取的组织样品的装置（10）和方法包括安装到框架（12）并连接到多个包含组织的组织样本容器（52）的往复运动元件（20）样品（62）和搅拌构件（60）。通过激活往复运动元件（20），组织样本（62）被搅拌构件（60）研磨。控制回路（18）连接到往复运动元件（20），用于控制搅拌的速度和持续时间。

7. 发明申请

WO1997042333A1 METHODS AND COMPOSITIONS FOR PRODUCTION OF PLANT FOODSTUFFS WITH ENHANCED SWEET COMPONENT OF FLAVOR 审中-公开
标题翻译：用于生产植物食品的方法和组合物与增强的甜味剂组分
公开(公告)号：WO1997042333A1
公开(公告)日：1997-11-13
申请号：PCT/US1997007877
申请日：1997-05-08
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： PIONEER HI-BRED INTERNATIONAL, INC. , TOMES, Dwight, T.
IPC分类号： C12N15/82
CPC分类号： C12N15/8242 , C07K14/43
摘要： The invention discloses a transgenic method for producing plant foodstuffs such as fruits, vegetables or seeds with a modified sweet component. It involves the temporal expression of a gene which encodes the sweet protein brazzein to enhance the sweet component of flavor. The invention also includes constructs, vectors, and transgenic plants for production of such foodstuffs.
摘要翻译：本发明公开了一种用于生产具有改性甜味成分的植物食品如水果，蔬菜或种子的转基因方法。它涉及编码甜蛋白brazzein的基因的时间表达，以增强风味的甜味成分。本发明还包括用于生产这种食品的构建体，载体和转基因植物。

8. 发明申请

WO2003026389A3 MANIPULATION OF PLANT POLYSACCHARIDE SYNTHASES 审中-公开
公开(公告)号：WO2003026389A3
公开(公告)日：2003-04-03
申请号：PCT/US2002/030796
申请日：2002-09-27
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： DHUGGA, Kanwarpal, S. , TOMES, Dwight, T.
IPC分类号： A01H1/00
摘要： The present invention provides compositions and methods for manipulation of plant polysaccharides and plant polysaccharide synthases. Compositions include novel nucleotide sequences encoding polysaccharide synthases polypeptides. and biologically active variants thereof. Further provided are methods for polysaccharide manipulation using the sequences disclosed herein. One method comprises stably incorporating into the genome of a plant cell. a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence. Methods for enhancing digestibility of plants. improving gum extractability from plants. and altering plant growth are also provided.

9. 发明申请

WO1996004392A2 TRANSGENIC CEREAL PLANTS 审中-公开
标题翻译：转基因谷物植物
公开(公告)号：WO1996004392A2
公开(公告)日：1996-02-15
申请号：PCT/US1995008977
申请日：1995-07-26
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： PIONEER HI-BRED INTERNATIONAL, INC. , BOWEN, Benjamin, A. , LOWE, Keith , ROSS, Margot, C. , SANDAHL, Gary, A. , TOMES, Dwight, T. , SONGSTAD, David, D. , GORDON-KAMM, William, J.
IPC分类号： C12N15/82
CPC分类号： C12N15/8207
摘要： To obtain a transgenic cereal plant which is stably transformed, an exposed cereal meristem is subjected to biolistic bombardment in order to target non-differentiated meristem cells for transformation. Immature embryos at the early proembryo, mid proembryo, late proembryo, transitional or early coleoptilar stage are harvested for biolistic bombardment. The meristem tissue or cells fated to contribute to the meristem then are manipulated in order to enlarge transgenic sectors, either through selection and/or through effecting a proliferation from the tissue of shoots or multiple meristems per se. The shoot population thus obtained then is screened, by means of a nonlethal enrichment assay, to identify either chimeric sectors that will contribute to germline transmission, or non-sectored, L2 periclinal chimeras that will by definition transmit to progeny. Increased time in culture, under selection, enhances the prospects for sectoral-to-periclinal conversions, and also selects for L1-to-L2 conversions which, through a shift in position, ultimately contribute to the germline. Transgenic sectors also are stabilized during the step of tillering.
摘要翻译：为了获得稳定转化的转基因谷物植物，将暴露的谷物分生组织进行生物射弹轰击，以靶向未分化的分生组织转化。收获早期胚胎，中胚胎，晚期胚胎，过渡期或早期胚胎发育阶段的未成熟胚胎进行生物射弹轰击。为了通过选择和/或通过实现来自组织或多个分生组织本身的增殖来扩大转基因部分，操纵分生组织或细胞，以促成分生组织。然后通过非致死浓缩测定筛选由此获得的芽种群，以鉴定将有助于种系传播的嵌合区段或将通过定义向子代传播的非分区的L2周边嵌合体。增加的文化时间在不断的选择下，增加了部门间的转化前景，并选择了L1到L2的转换，通过转变，最终有助于种系。转基因部门在分蘖期间也稳定下来。

10. 发明申请

WO2003026389A2 MANIPULATION OF PLANT POLYSACCHARIDE SYNTHASES 审中-公开
标题翻译：植物多糖的合成操作
公开(公告)号：WO2003026389A2
公开(公告)日：2003-04-03
申请号：PCT/US2002/030796
申请日：2002-09-27
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： DHUGGA, Kanwarpal, S. , TOMES, Dwight, T.
IPC分类号： A01H
CPC分类号： C12N9/1059 , C12N15/8246
摘要： The present invention provides compositions and methods for manipulation of plant polysaccharides and plant polysaccharide synthases. Compositions include novel nucleotide sequences encoding polysaccharide synthases polypeptides. and biologically active variants thereof. Further provided are methods for polysaccharide manipulation using the sequences disclosed herein. One method comprises stably incorporating into the genome of a plant cell. a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence. Methods for enhancing digestibility of plants. improving gum extractability from plants. and altering plant growth are also provided.
摘要翻译：本发明提供了用于操作植物多糖和植物多糖合酶的组合物和方法。组合物包括编码多糖合酶多肽的新型核苷酸序列。和其生物活性变体。进一步提供了使用本文公开的序列进行多糖操作的方法。一种方法包括稳定掺入植物细胞的基因组。本发明的核苷酸序列可操作地连接到异源启动子并再生表达核苷酸序列的稳定转化的植物。提高植物消化率的方法。改善植物的树胶提取能力。并改变植物生长。

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