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1. 发明申请

WO1996004392A2 TRANSGENIC CEREAL PLANTS 审中-公开
标题翻译：转基因谷物植物
公开(公告)号：WO1996004392A2
公开(公告)日：1996-02-15
申请号：PCT/US1995008977
申请日：1995-07-26
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： PIONEER HI-BRED INTERNATIONAL, INC. , BOWEN, Benjamin, A. , LOWE, Keith , ROSS, Margot, C. , SANDAHL, Gary, A. , TOMES, Dwight, T. , SONGSTAD, David, D. , GORDON-KAMM, William, J.
IPC分类号： C12N15/82
CPC分类号： C12N15/8207
摘要： To obtain a transgenic cereal plant which is stably transformed, an exposed cereal meristem is subjected to biolistic bombardment in order to target non-differentiated meristem cells for transformation. Immature embryos at the early proembryo, mid proembryo, late proembryo, transitional or early coleoptilar stage are harvested for biolistic bombardment. The meristem tissue or cells fated to contribute to the meristem then are manipulated in order to enlarge transgenic sectors, either through selection and/or through effecting a proliferation from the tissue of shoots or multiple meristems per se. The shoot population thus obtained then is screened, by means of a nonlethal enrichment assay, to identify either chimeric sectors that will contribute to germline transmission, or non-sectored, L2 periclinal chimeras that will by definition transmit to progeny. Increased time in culture, under selection, enhances the prospects for sectoral-to-periclinal conversions, and also selects for L1-to-L2 conversions which, through a shift in position, ultimately contribute to the germline. Transgenic sectors also are stabilized during the step of tillering.
摘要翻译：为了获得稳定转化的转基因谷物植物，将暴露的谷物分生组织进行生物射弹轰击，以靶向未分化的分生组织转化。收获早期胚胎，中胚胎，晚期胚胎，过渡期或早期胚胎发育阶段的未成熟胚胎进行生物射弹轰击。为了通过选择和/或通过实现来自组织或多个分生组织本身的增殖来扩大转基因部分，操纵分生组织或细胞，以促成分生组织。然后通过非致死浓缩测定筛选由此获得的芽种群，以鉴定将有助于种系传播的嵌合区段或将通过定义向子代传播的非分区的L2周边嵌合体。增加的文化时间在不断的选择下，增加了部门间的转化前景，并选择了L1到L2的转换，通过转变，最终有助于种系。转基因部门在分蘖期间也稳定下来。

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