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    • 3. 发明申请
    • MICROORGANISMS FOR PRODUCING METHACRYLIC ACID AND METHACRYLATE ESTERS AND METHODS RELATED THERETO
    • 生产甲基丙烯酸和甲基丙烯酸酯的微生物及其相关方法
    • WO2012135789A2
    • 2012-10-04
    • PCT/US2012/031733
    • 2012-03-30
    • GENOMATICA, INC.BURK, Mark, J.BURGARD, Anthony, P.OSTERHOUT, Robin, E.SUN, JunPHARKYA, Priti
    • BURK, Mark, J.BURGARD, Anthony, P.OSTERHOUT, Robin, E.SUN, JunPHARKYA, Priti
    • C12P7/40
    • C12P7/40C12P7/42C12P7/62C12P19/32
    • The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2- hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.
    • 本发明提供具有甲基丙烯酸,甲基丙烯酸酯,3-羟基异丁酸酯和/或2-羟基异丁酸酯途径的非天然存在的微生物生物。 微生物生物体含有编码甲基丙烯酸途径中的酶的至少一种外源核酸。 本发明另外提供了生产甲基丙烯酸,甲基丙烯酸酯,3-羟基异丁酸酯和/或2-羟基异丁酸酯的方法。 该方法可以包括培养产生甲基丙烯酸,甲基丙烯酸酯,3-羟基异丁酸酯和/或2-羟基异丁酸酯的微生物,其中微生物生物体表达至少一种编码甲基丙烯酸途径酶的外源核酸,其量足以产生甲基丙烯酸, 甲基丙烯酸酯,3-羟基异丁酸酯和/或2-羟基异丁酸酯在条件下和足够的时间以产生甲基丙烯酸,甲基丙烯酸酯,3-羟基异丁酸酯和/或2-羟基异丁酸酯。
    • 8. 发明申请
    • ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE
    • 生产环己酮的有机体
    • WO2010132845A1
    • 2010-11-18
    • PCT/US2010/035015
    • 2010-05-14
    • GENOMATICA, INC.OSTERHOUT, Robin, E.BURGARD, Anthony, P.BURK, Mark, J.PHARKYA, Priti
    • OSTERHOUT, Robin, E.BURGARD, Anthony, P.BURK, Mark, J.PHARKYA, Priti
    • A61K48/00C07C49/00
    • C12P7/26C12N9/16C12N9/88C12N9/93C12N15/52C12N15/70
    • A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1 -carboxyl-CoA hydrolase (acting on C-C bond), a 2-ketocyclohexane-l- carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-l-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-l -carboxyl-CoA transferase, and a 2- ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6- ketocyclohex-1-ene-l-carboxyl-CoA hydrolase (acting on C-C bond), a 6-ketocyclohex- 1 -ene- 1 - carboxyl-CoA synthetase, a 6-ketocyclohex-l-ene-l-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex- 1 -ene- 1 -carboxyl-CoA transferase, a 6-ketocyclohex- 1 -ene- 1 - carboxyl-CoA reductase, a 6-ketocyclohex- 1 -ene- 1 -carboxylate decarboxylase, a 6- ketocyclohex-1 -ene- 1 -carboxylate reductase, a 2-ketocyclohexane-l-carboxyl-CoA synthetase, a 2-ketocyclohexane-l -carboxyl-CoA transferase, a 2-ketocyclohexane-l-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-l -carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2- diol dehydrogenase, and a cyclohexane-l,2-diol dehydratase. A pathway includes a 3- oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.
    • 非天然存在的微生物有机体具有包含至少一种编码环己酮途径酶的外源核酸的环己酮途径。 途径包括2-酮环己烷-1-羧基-CoA水解酶(作用于CC键),2-酮环己烷-1-羧酸脱羧酶和选自2-酮环己烷-1-羧基-CoA水解酶(酶作用于硫酯) ),2-酮环己烷-1-羧基-CoA转移酶和2-酮环己烷-1-羧基-CoA合成酶。 途径包括选自6-酮环己-1-烯-1-羧基-CoA水解酶(作用于CC键)的酶,6-酮环己-1-烯-1-羧基-CoA合成酶,6-酮环己基 - 1-烯-1-羧基-CoA水解酶(作用于硫酯),6-酮环己-1-烯-1-羧基-CoA转移酶,6-酮环己-1-烯-1-羧基-CoA还原酶,6 2-环己-1-烯-1-羧酸脱羧酶,6-酮环己-1-烯-1-羧酸还原酶,2-酮环己烷-1-羧基-CoA合成酶,2-酮环己烷-1-羧基-CoA转移酶, 2-酮环己烷-1-羧基-CoA水解酶(作用于硫酯),2-酮环己烷-1-羧酸脱羧酶和环己酮脱氢酶。 途径包括己二酸半醛脱水酶,环己烷-1,2-二醇脱氢酶和环己烷-1,2-二醇脱水酶。 途径包括3-氧代庚酸酯脱羧酶,4-乙酰基丁酸脱水酶,3-羟基环己酮脱氢酶,2-环己烯酮水合酶,环己酮脱氢酶和选自3-氧代木糖酰辅酶A合成酶,3-氧代木糖酰-CoA水解酶 (作用于硫酯)和3-氧代木糖酰辅酶A转移酶。 每个这些途径可以包括PEP羧基激酶。 生产环己酮的方法包括培养这些非天然存在的微生物。