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1. 发明申请

WO2012177619A2 MICROORGANISMS FOR PRODUCING 1,3-BUTANEDIOL AND METHODS RELATED THERETO 审中-公开
标题翻译：用于生产1,3-丁二醇的微生物及其相关方法
公开(公告)号：WO2012177619A2
公开(公告)日：2012-12-27
申请号：PCT/US2012/043117
申请日：2012-06-19
申请人： GENOMATICA, INC. , BURGARD, Anthony, P. , BURK, Mark, J. , OSTERHOUT, Robin, E. , SUN, Jun , PHARKYA, Priti
发明人： BURGARD, Anthony, P. , BURK, Mark, J. , OSTERHOUT, Robin, E. , SUN, Jun , PHARKYA, Priti
IPC分类号： C12N1/21 , C12P7/18
CPC分类号： C12P7/18 , C12N15/52
摘要： Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3 -BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3 -BDO pathway enzyme expressed in a sufficient amount to produce 1,3 -BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.
摘要翻译：本文提供了具有1,3-丁二醇（1,3-BOD）途径的非天然存在的微生物生物，并且包含至少一种编码1,3 -BDO途径酶的外源核酸，其以足够的量表达以产生1 ，3 -BDO。在一些实施方案中，该途径包括从CO或氢中还原当量。在某些实施方案中，1,3-BDO途径通过中枢代谢物丙酮酸，琥珀酸或α-酮戊二酸进行。本文还提供了一种生产1,3-BDO的方法，包括在条件和足够的时间内培养这些微生物以产生1,3-BDO。

2. 发明申请

WO2012135789A2 MICROORGANISMS FOR PRODUCING METHACRYLIC ACID AND METHACRYLATE ESTERS AND METHODS RELATED THERETO 审中-公开
标题翻译：生产甲基丙烯酸和甲基丙烯酸酯的微生物及其相关方法
公开(公告)号：WO2012135789A2
公开(公告)日：2012-10-04
申请号：PCT/US2012/031733
申请日：2012-03-30
申请人： GENOMATICA, INC. , BURK, Mark, J. , BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , SUN, Jun , PHARKYA, Priti
发明人： BURK, Mark, J. , BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , SUN, Jun , PHARKYA, Priti
IPC分类号： C12P7/40
CPC分类号： C12P7/40 , C12P7/42 , C12P7/62 , C12P19/32
摘要： The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2- hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.
摘要翻译：本发明提供具有甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯途径的非天然存在的微生物生物。微生物生物体含有编码甲基丙烯酸途径中的酶的至少一种外源核酸。本发明另外提供了生产甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的方法。该方法可以包括培养产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的微生物，其中微生物生物体表达至少一种编码甲基丙烯酸途径酶的外源核酸，其量足以产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯在条件下和足够的时间以产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯。

3. 发明申请

WO2012018624A3 MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF AROMATICS, 2,4-PENTADIENOATE AND 1,3-BUTADIENE 审中-公开
公开(公告)号：WO2012018624A3
公开(公告)日：2012-02-09
申请号：PCT/US2011/045364
申请日：2011-07-26
申请人： GENOMATICA, INC. , OSTERHOUT, Robin, E. , BURGARD, Anthony, P. , PHARKYA, Priti , BURK, Mark, J.
发明人： OSTERHOUT, Robin, E. , BURGARD, Anthony, P. , PHARKYA, Priti , BURK, Mark, J.
IPC分类号： C12P5/00 , C12P5/02 , C12P7/40
摘要： The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-lol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4- oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4- pentadienoate, 3-butene-lol or 1,3-butadiene.

4. 发明申请

WO2010132845A1 ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE 审中-公开
标题翻译：生产环己酮的有机体
公开(公告)号：WO2010132845A1
公开(公告)日：2010-11-18
申请号：PCT/US2010/035015
申请日：2010-05-14
申请人： GENOMATICA, INC. , OSTERHOUT, Robin, E. , BURGARD, Anthony, P. , BURK, Mark, J. , PHARKYA, Priti
发明人： OSTERHOUT, Robin, E. , BURGARD, Anthony, P. , BURK, Mark, J. , PHARKYA, Priti
IPC分类号： A61K48/00 , C07C49/00
CPC分类号： C12P7/26 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/52 , C12N15/70
摘要： A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1 -carboxyl-CoA hydrolase (acting on C-C bond), a 2-ketocyclohexane-l- carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-l-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-l -carboxyl-CoA transferase, and a 2- ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6- ketocyclohex-1-ene-l-carboxyl-CoA hydrolase (acting on C-C bond), a 6-ketocyclohex- 1 -ene- 1 - carboxyl-CoA synthetase, a 6-ketocyclohex-l-ene-l-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex- 1 -ene- 1 -carboxyl-CoA transferase, a 6-ketocyclohex- 1 -ene- 1 - carboxyl-CoA reductase, a 6-ketocyclohex- 1 -ene- 1 -carboxylate decarboxylase, a 6- ketocyclohex-1 -ene- 1 -carboxylate reductase, a 2-ketocyclohexane-l-carboxyl-CoA synthetase, a 2-ketocyclohexane-l -carboxyl-CoA transferase, a 2-ketocyclohexane-l-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-l -carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2- diol dehydrogenase, and a cyclohexane-l,2-diol dehydratase. A pathway includes a 3- oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.
摘要翻译：非天然存在的微生物有机体具有包含至少一种编码环己酮途径酶的外源核酸的环己酮途径。途径包括2-酮环己烷-1-羧基-CoA水解酶（作用于CC键），2-酮环己烷-1-羧酸脱羧酶和选自2-酮环己烷-1-羧基-CoA水解酶（酶作用于硫酯）），2-酮环己烷-1-羧基-CoA转移酶和2-酮环己烷-1-羧基-CoA合成酶。途径包括选自6-酮环己-1-烯-1-羧基-CoA水解酶（作用于CC键）的酶，6-酮环己-1-烯-1-羧基-CoA合成酶，6-酮环己基 - 1-烯-1-羧基-CoA水解酶（作用于硫酯），6-酮环己-1-烯-1-羧基-CoA转移酶，6-酮环己-1-烯-1-羧基-CoA还原酶，6 2-环己-1-烯-1-羧酸脱羧酶，6-酮环己-1-烯-1-羧酸还原酶，2-酮环己烷-1-羧基-CoA合成酶，2-酮环己烷-1-羧基-CoA转移酶， 2-酮环己烷-1-羧基-CoA水解酶（作用于硫酯），2-酮环己烷-1-羧酸脱羧酶和环己酮脱氢酶。途径包括己二酸半醛脱水酶，环己烷-1,2-二醇脱氢酶和环己烷-1,2-二醇脱水酶。途径包括3-氧代庚酸酯脱羧酶，4-乙酰基丁酸脱水酶，3-羟基环己酮脱氢酶，2-环己烯酮水合酶，环己酮脱氢酶和选自3-氧代木糖酰辅酶A合成酶，3-氧代木糖酰-CoA水解酶（作用于硫酯）和3-氧代木糖酰辅酶A转移酶。每个这些途径可以包括PEP羧基激酶。生产环己酮的方法包括培养这些非天然存在的微生物。

5. 发明申请

WO2011031897A1 MICROORGANISMS AND METHODS FOR THE CO-PRODUCTION OF ISOPROPANOL WITH PRIMARY ALCOHOLS, DIOLS AND ACIDS 审中-公开
标题翻译：用主要醇，二醇和酸共同生产异丙酚的微生物和方法
公开(公告)号：WO2011031897A1
公开(公告)日：2011-03-17
申请号：PCT/US2010/048318
申请日：2010-09-09
申请人： GENOMATICA, INC. , PHARKYA, Priti , BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , BURK, Mark, J. , SUN, Jun
发明人： PHARKYA, Priti , BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , BURK, Mark, J. , SUN, Jun
IPC分类号： C12P7/04 , C12N15/74
CPC分类号： C12P7/40 , C12N15/52 , C12P7/04 , C12P7/18
摘要： The invention provides a non-naturally occurring microbial organism having n-propanol and isopropanol pathways, 1,4-butanediol (14-BDO) and isopropanol pathways, 1,3-butanediol (13- BDO) and isopropanol pathways or methylacrylic acid (MAA) and isopropanolpathways. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in each of the respective n-propanol, 14-BDO, 13-BDO or MAA and isopropanol pathways. The invention additionally provides a method for co-producing n-propanol and isopropanol, 14-BDO and isopropanol, 13-BDO and isopropanol or MAA and isopropanol. The method can include culturing an n-propanol and an isopropanol co-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an n-propanol, an isopropanol, a 14-BDO, a 13-BDO and/or a MAA pathway enzyme in a sufficient amount to produce each of the respective products, under conditions and for a sufficient period of time to produce each of the respective products.
摘要翻译：本发明提供具有正丙醇和异丙醇途径的非天然存在的微生物生物，1,4-丁二醇（14-BDO）和异丙醇途径，1,3-丁二醇（13-BDO）和异丙醇途径或甲基丙烯酸（MAA ）和异丙醇通道。微生物生物体含有至少一种外源核酸，其编码在各自的正丙醇，14-BDO，13-BDO或MAA和异丙醇途径中的酶。本发明另外提供了共同生产正丙醇和异丙醇，14-BDO和异丙醇，13-BDO和异丙醇或MAA和异丙醇的方法。该方法可以包括培养正丙醇和异丙醇共同生产微生物，其中微生物生物体表达至少一种编码正丙醇，异丙醇，14-BDO，13-BDO和/或足够量的MAA途径酶，以在产生各个产物的条件下和足够的时间段内产生各个产物。

6. 发明申请

WO2010129936A1 MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF ADIPATE, HEXAMETHYLENEDIAMINE AND 6-AMINOCAPROIC ACID 审中-公开
标题翻译：丙二酸，十六烷二胺和6-氨基酸的生物合成的微生物和方法
公开(公告)号：WO2010129936A1
公开(公告)日：2010-11-11
申请号：PCT/US2010/034144
申请日：2010-05-07
申请人： GENOMATICA, INC. , BURK, Mark, J. , BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , PHARKYA, Priti
发明人： BURK, Mark, J. , BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , PHARKYA, Priti
IPC分类号： C12N1/21 , C12P13/04 , C12P7/44
CPC分类号： C12P17/10 , C12N1/38 , C12N15/52 , C12P7/40 , C12P7/44 , C12P13/001 , C12P13/005 , C12P13/02 , C12P17/12
摘要： The invention provides a non-naturally occurring microbial organism having a 6- aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.
摘要翻译：本发明提供了具有6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径的非天然存在的微生物。微生物生物体含有至少一种编码各自的6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径中的酶的外源核酸。本发明另外提供了制备6-氨基己酸，己内酰胺，己二胺或乙酰丙酸的方法。该方法可以包括培养6-氨基己酸，己内酰胺或六亚甲基二胺生产微生物，其中微生物生物表达至少一种编码6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸途径酶的外源核酸，其量足以产生各自的产物在条件下和足够的时间内产生6-氨基己酸，己内酰胺，六亚甲基二胺或乙酰丙酸。

7. 发明申请

WO2009135074A3 MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID 审中-公开
公开(公告)号：WO2009135074A3
公开(公告)日：2009-11-05
申请号：PCT/US2009/042426
申请日：2009-04-30
申请人： GENOMATICA, INC. , BURK, Mark, J. , BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , PHARKYA, Priti
发明人： BURK, Mark, J. , BURGARD, Anthony, P. , OSTERHOUT, Robin, E. , PHARKYA, Priti
IPC分类号： C12P7/42 , C12N1/20
摘要： The invention provides a non-naturally occurring microbial organism having a 2- hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2- hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3- hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.

8. 发明申请

WO2010127319A2 ORGANISMS FOR THE PRODUCTION OF 1,3-BUTANEDIOL 审中-公开
标题翻译：用于生产1,3-丁二醇的有机物
公开(公告)号：WO2010127319A2
公开(公告)日：2010-11-04
申请号：PCT/US2010/033300
申请日：2010-04-30
申请人： GENOMATICA, INC. , BURGARD, Anthony, P. , BURK, Mark, J. , OSTERHOUT, Robin, E. , PHARKYA, Priti
发明人： BURGARD, Anthony, P. , BURK, Mark, J. , OSTERHOUT, Robin, E. , PHARKYA, Priti
IPC分类号： C12N1/21 , C12P7/18
CPC分类号： C12P7/18 , C07C29/80 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12N9/1096 , C12N9/88 , C12N15/52 , C12Y101/01 , C12Y101/01157 , C12Y102/01 , C12Y203/01 , C12Y206/01 , C12Y401/01 , C12Y402/01 , C12Y403/01
摘要： A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3- BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4- hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2- one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA- dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl- CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.
摘要翻译：非天然存在的微生物有机体包括具有1,3-丁二醇（1,3-BDO）途径的微生物，其具有至少一种编码1,3-BDO途径酶的外源核酸以足以产生1,3-BDO的量表达。该途径包括选自2-氨基-4-酮戊酸（AKP）硫解酶，AKP脱氢酶，2-氨基-4-羟基戊酸氨基转移酶，2-氨基-4-羟基戊酸酯氧化还原酶（脱氨基），2-氨基-4-羟基戊酸 4-羟基戊酸脱羧酶，3-羟基丁醛还原酶，AKP氨基转移酶，AKP氧化还原酶（脱氨基），2,4-二氧代戊酸脱羧酶，3-氧代丁醛还原酶（酮还原），3-氧代丁醛还原酶（醛还原），4-羟基-2-丁酮还原酶，AKP脱羧酶，4-氨基丁-2-酮转氨酶，4-氨基丁-2-酮氧化还原酶（脱氨基），4-氨基丁-2-酮氨裂解酶，丁烯酮水合酶，AKP氨 - 裂解酶，乙酰基丙烯酸酯脱羧酶，乙酰乙酰-CoA还原酶（CoA依赖性，醛形成），乙酰乙酰-CoA还原酶（CoA依赖性，形成醇），乙酰乙酰-CoA还原酶（酮还原），3-羟基丁酰-CoA还原酶（醛形成），3-羟基乌乙酰-CoA还原酶（形成醇），4-羟基丁酰-CoA脱水酶和巴豆酸酶。生产1,3-BDO的方法包括在一定条件下培养这种微生物并持续足够的时间以产生1,3-BDO。

9. 发明申请

WO2010057022A1 MICROORGANISMS FOR THE PRODUCTION OF METHYL ETHYL KETONE AND 2-BUTANOL 审中-公开
标题翻译：用于生产甲基乙基酮和2-丁醇的微生物
公开(公告)号：WO2010057022A1
公开(公告)日：2010-05-20
申请号：PCT/US2009/064433
申请日：2009-11-13
申请人： GENOMATICA, INC. , BURK, Mark, J. , PHARKYA, Priti , BURGARD, Anthony, P.
发明人： BURK, Mark, J. , PHARKYA, Priti , BURGARD, Anthony, P.
IPC分类号： C12P7/26 , C12P7/00 , C12N1/21
CPC分类号： C12P7/16 , C12N15/52 , C12P7/26 , Y02E50/10
摘要： A non-naturally occurring microbial organism having a methyl ethyl ketone pathway includes at least one exogenous nucleic acid encoding a methyl ethyl ketone pathway enzyme expressed in a sufficient amount to produce methyl ethyl ketone. The methyl ethyl ketone pathway includes a β-ketothiolase, a β-ketovalerate decarboxylase and an enzyme selected from the group consisting of a β-ketovaleryl-CoA hydrolase and a β-ketovaleryl-CoA transferase. Alternatively, the methyl ethyl ketone pathway includes a 2-methylacetoacetyl-CoA thiolase, a 2-methylacetoacetate decarboxylase and an enzyme selected from the group consisting of a 2-methylacetoacetyl-CoA hydrolase and a 2-methylacetoacetyl-CoA transferase. Either pathway can further include a methyl ethyl ketone reductase to produce 2-BuOH. A method for producing methyl ethyl ketone or 2-BuOH includes culturing these non-naturally occurring microbial organisms under conditions, and for a sufficient period of time, to produce methyl ethyl ketone or 2-BuOH.
摘要翻译：具有甲基乙基酮途径的非天然存在的微生物包括至少一种编码甲基乙基酮途径酶的外源核酸，其以足以产生甲基乙基酮的量表达。甲基乙基酮途径包括β-酮硫解酶，β-酮戊二酸脱羧酶和选自β-酮戊酰基-CoA水解酶和β-酮戊酰基-CoA转移酶的酶。或者，甲基乙基酮途径包括2-甲基乙酰乙酰辅酶A硫解酶，2-甲基乙酰乙酸脱羧酶和选自2-甲基乙酰乙酰辅酶A水解酶和2-甲基乙酰乙酰辅酶A转移酶的酶。任一途径还可以包括甲基乙基酮还原酶以产生2-BuOH。一种生产甲基乙基酮或2-BuOH的方法包括在条件和足够的时间内培养这些非天然存在的微生物生产甲基乙基酮或2-BuOH。

10. 发明申请

WO2009045637A9 METHODS FOR THE SYNTHESIS OF OLEFINS AND DERIVATIVES 审中-公开
公开(公告)号：WO2009045637A9
公开(公告)日：2009-04-09
申请号：PCT/US2008/072679
申请日：2008-08-08
申请人： GENOMATICA, INC. , BURK, Mark, J. , PHARKYA, Priti , VAN DIEN, Stephen, J. , BURGARD, Anthony, P. , SCHILLING, Christopher, H.
发明人： BURK, Mark, J. , PHARKYA, Priti , VAN DIEN, Stephen, J. , BURGARD, Anthony, P. , SCHILLING, Christopher, H.
IPC分类号： C07C51/347 , C07C67/333 , C12P7/46 , C07C57/04 , C07C69/54
摘要： The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

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