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1. 发明申请

WO2013083482A1 CHROMATOGRAPHIC METHOD FOR THE SEPARATION OF FATTY ACID MIXTURES 审中-公开
标题翻译：用于分离脂肪酸混合物的色谱方法
公开(公告)号：WO2013083482A1
公开(公告)日：2013-06-13
申请号：PCT/EP2012/074047
申请日：2012-11-30
申请人： CHROMACON AG , MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael
发明人： MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael
IPC分类号： G01N30/42 , G01N30/44 , G01N30/46 , B01D15/18 , C11B7/00 , G01N30/80
CPC分类号： G01N30/42 , B01D15/166 , B01D15/1814 , B01D15/1864 , C11B7/00 , G01N30/44 , G01N30/468 , G01N30/80
摘要： The invention relates to a process for purification of a fatty acid containing, multi-component mixture (F) by means of at least two individual chromatographic columns through which the mixture (F) is fed by means of at least one solvent (s). According to the invention, the multi- component mixture (F) at least comprises weakly adsorbing impurities (W), a first target product (P1)to be purified, an intermediate impurity (S1), a second target product (P2) to be purified, and strongly adsorbing impurities (S2), the first target product (P1) is first fatty acid, e.g. eicosapentaenoic acid (EPA), or a derivative thereof, and the second target product (P2) is a second fatty acid different from the first target product (P1), or a derivative of said second fatty acid. For the purification a multicolumn countercurrent purification chromatography is used, and in this multicolumn countercurrent purification chromatography at least one outlet thereof is further separated by using outlet fractionation.
摘要翻译：本发明涉及通过至少两个单独的色谱柱纯化含有脂肪酸的多组分混合物（F）的方法，混合物（F）通过至少一种溶剂通过至少一种溶剂进料。根据本发明，多组分混合物（F）至少包含弱吸附杂质（W），待纯化的第一目标产物（P1），中间杂质（S1），第二目标产物（P2）纯化和强吸附杂质（S2），第一目标产物（P1）是第一种脂肪酸，例如，二十碳五烯酸（EPA）或其衍生物，第二目标产物（P2）是与第一目标产物（P1）不同的第二种脂肪酸或所述第二种脂肪酸的衍生物。对于纯化，使用多列逆流纯化色谱法，并且在该多列逆流纯化色谱中，通过使用出口分馏进一步分离其至少一个出口。

2. 发明申请

WO2012123520A1 METHOD FOR IDENTIFICATION AND PURIFICATION OF MULTI-SPECIFIC POLYPEPTIDES 审中-公开
标题翻译：多特异性多肽的鉴定和纯化方法
公开(公告)号：WO2012123520A1
公开(公告)日：2012-09-20
申请号：PCT/EP2012/054507
申请日：2012-03-15
申请人： CHROMACON AG , MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael
发明人： MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael
IPC分类号： B01D15/18 , G01N30/46
CPC分类号： C12N15/1082 , B01D15/166 , B01D15/1807 , B01D15/1864 , C07K1/18 , C07K1/22 , C07K1/36 , C07K2317/31 , G01N30/468
摘要： The document pertains to a method for the purification of a ternary mixture of dimeric antibodies of the type AA, AB, BB, characterised in that for the separation of the three components and in particular for the isolation of the multi-specific fraction ABmulticolumn counter current solvent gradient purification chromatography with a stationary phase load of more than 1 mg antibody mixture per millilitre stationary phase is used.It furthermore relates to a method for the identification of in particular bispecific antibody systems, which are particularly suitable for the application of such a purification method.
摘要翻译：该文献涉及一种用于纯化AA，AB，BB型二聚体抗体三元混合物的方法，其特征在于用于分离三种成分，特别是用于分离多特异性级分的多元柱反向电流使用具有大于1mg抗体混合物/毫升固定相的固定相负载的溶剂梯度纯化色谱法。此外，还涉及用于特异性鉴定特异性双特异性抗体系统的方法，其特别适用于施用这种纯化方法。

3. 发明申请

WO2019096622A1 METHOD FOR MONITORING, EVALUATING, AND CONTROLLING A CYCLIC CHROMATOGRAPHIC PURIFICATION PROCESS 审中-公开
公开(公告)号：WO2019096622A1
公开(公告)日：2019-05-23
申请号：PCT/EP2018/080261
申请日：2018-11-06
申请人： CHROMACON AG
发明人： AUMANN, Lars , MÜLLER-SPÄTH, Thomas , BAVAND, Michael
IPC分类号： B01D15/18 , G01N30/60 , G01N30/86
摘要： A method for monitoring, evaluating and controlling a cyclic chromatographic purification process is proposed involving at least two adsorbers, wherein the method comprises at least the following steps: a) monitoring of the chromatogram including the measurement of at least one current concentration-proportional signal in the liquid; b) evaluation of the chromatogram including a comparison of at least one of said current concentration-proportional signals measured in step (a) with a threshold value thereof; c) controlling the chromatographic purification process by adapting the termination of the currently running phase as a function of the comparison of step (b) and initiating the next phase, wherein the sequence a)-c) is carried out in given order at least twice.

4. 发明申请

WO2014131719A1 CHROMATOGRAPHIC PROCESS FOR ENRICHMENT AND ISOLATION 审中-公开
标题翻译：用于增强和分离的色谱方法
公开(公告)号：WO2014131719A1
公开(公告)日：2014-09-04
申请号：PCT/EP2014/053511
申请日：2014-02-24
申请人： CHROMACON AG
发明人： MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael , ULMER, Nicole
IPC分类号： B01D15/18 , G01N30/46
CPC分类号： C07K1/165 , B01D15/1864 , B01D15/40 , C11B3/008 , G01N30/461 , G01N30/466 , G01N30/468
摘要： A chromatographic process for the enrichment of at least one compound of interest (X) from a mixture (F) comprising said at least one compound of interest (X) as well as at least one further compound (W, C, S), which is not of interest, is proposed, using at least two chromatographic columns, wherein said process at least involves a sequence of the following steps: (i) a cyclic accumulation phase, in which the at least two chromatographic columns are alternatingly operated in an interconnected phase (IC), followed by a disconnected phase (B), wherein after these two phases the first and second column or group of columns exchange places; wherein said two phases are carried out sequentially M times with M > 1; (ii) a cyclic separation phase, in which the at least two chromatographic columns are alternatingly operated in an interconnected phase (IC), followed by a disconnected phase (B), wherein after these two phases the first and second column or group of columns exchange places to undergo the next interconnected (IC) and disconnected phases (B); wherein said two phases are carried out sequentially N times with N > 0; (iii) an elution phase, in which from the column or group of columns, which at the end of phase (i) or, in case of N>0 at the end of phase (ii) contains the at least one compound of interest (X), said at least one compound of interest (X) is extracted via the outlet.
摘要翻译：用于从包含所述至少一种目标化合物（X）的混合物（F）以及至少一种其它化合物（W，C，S）富集至少一种目的化合物（X）的色谱方法，其中提出使用至少两个色谱柱，其中所述方法至少涉及以下步骤的顺序：（i）循环累积阶段，其中所述至少两个色谱柱交替地在互连的操作中相位（IC），之后是断开相位（B），其中在这两个阶段之后，第一和第二列或一组列交换位置; 其中所述两相按M> 1顺序执行M次; （ii）循环分离阶段，其中至少两个色谱柱在互连相（IC）中交替操作，随后是断开相（B），其中在这两相之后，第一和第二列或一组柱交换场所进行下一个互连（IC）和断开阶段（B）; 其中所述两相按N> 0顺序进行N次执行; （iii）洗脱阶段，其中在阶段（i）结束时或在阶段（ii）结束时的N> 0的情况下从柱或组列中含有至少一种目标化合物（X），所述至少一种感兴趣的化合物（X）经由所述出口提取。

5. 发明申请

WO2006094530A1 DI-POLYMER PROTEIN CONJUGATES AND PROCESSES FOR THEIR PREPARATION 审中-公开
标题翻译： DI聚合蛋白结合物及其制备方法
公开(公告)号：WO2006094530A1
公开(公告)日：2006-09-14
申请号：PCT/EP2005/002632
申请日：2005-03-11
申请人： SIEGFRIED LTD. , BAVAND, Michael , BLASEY, Horst , LANG, Dietmar
发明人： BAVAND, Michael , BLASEY, Horst , LANG, Dietmar
IPC分类号： C07K14/535 , A61K47/48
CPC分类号： C07K14/535 , A61K9/0019 , A61K47/60
摘要： The invention relates to polymer-protein conjugates, wherein two nitrogen atoms of amino groups of the protein are each conjugated with a polymer unit via an amine linkage, and to processes for their preparation.
摘要翻译：本发明涉及聚合物 - 蛋白质缀合物，其中蛋白质的氨基的两个氮原子通过胺键与聚合物单元缀合，并且其制备方法。

6. 发明申请

WO2006095029A2 DI-POLYMER PROTEIN CONJUGATES AND PROCESSES FOR THEIR PREPARATION 审中-公开
标题翻译： DI聚合蛋白结合物及其制备方法
公开(公告)号：WO2006095029A2
公开(公告)日：2006-09-14
申请号：PCT/EP2006/060671
申请日：2006-03-13
申请人： SIEGFRIED Ltd. , BAVAND, Michael , BLASEY, Horst , LANG, Dietmar
发明人： BAVAND, Michael , BLASEY, Horst , LANG, Dietmar
IPC分类号： C07K14/535 , A61K47/48
CPC分类号： C07K14/535 , A61K9/0019 , A61K47/60
摘要： The present invention refers to di-polymer protein conjugates and processes for their preparation. Further, the present invention refers to the use of such di-polymer protein conjugate, especially di-pegylated protein conjugate, for the manufacture of a medicament for the treatment of disorders.
摘要翻译：本发明涉及二聚体蛋白质缀合物及其制备方法。此外，本发明涉及这种二聚体蛋白结合物，特别是二聚乙二醇化蛋白质缀合物在制备用于治疗疾病的药物中的用途。

7. 发明申请

WO2018011102A1 PURIFICATION METHOD AND USES THEREOF 审中-公开
标题翻译：净化方法及其用途
公开(公告)号：WO2018011102A1
公开(公告)日：2018-01-18
申请号：PCT/EP2017/067190
申请日：2017-07-10
申请人： CHROMACON AG
发明人： MÜLLER-SPÄTH, Thomas , AUMANN, Lars , BAVAND, Michael
IPC分类号： B01D15/18 , G01N30/60
摘要： A cyclic chromatographic purification method for the isolation of a product from a feed mixture consisting of the product and at least one further component representing impurities, which impurities bind stronger to the chromatographic stationary phase than the product is given. The method uses at least two chromatographic adsorbers as chromatographic stationary phase, grouped into only one first adsorber section (1) and one second adsorber section (2), wherein if an adsorber section comprises more than one chromatographic adsorber these are permanently connected in series, wherein the first adsorber section (1) has a first adsorber section inlet and a first adsorber section outlet, and the second adsorber section (1) has a second adsorber section inlet and asecond adsorber section outlet.
摘要翻译：用于从由产物和代表杂质的至少一种其他组分组成的进料混合物中分离产物的循环色谱纯化方法，所述杂质与产物相比对色谱固定相结合更强。该方法使用至少两个色谱吸附器作为色谱固定相，仅分为一个第一吸附器部分（1）和一个第二吸附器部分（2），其中如果吸附剂部分包括多于一个色谱吸附器，则这些吸附器串联永久连接，其中第一吸附器部分（1）具有第一吸附器部分入口和第一吸附器部分出口，第二吸附器部分（1）具有第二吸附器部分入口和第二吸附器部分出口。

8. 发明申请

WO2014166799A1 CHROMATOGRAPHIC PURIFICATION METHOD 审中-公开
标题翻译：色谱纯化方法
公开(公告)号：WO2014166799A1
公开(公告)日：2014-10-16
申请号：PCT/EP2014/056656
申请日：2014-04-03
申请人： CHROMACON AG
发明人： MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael , ULMER, Nicole
IPC分类号： B01D15/18 , G01N30/42 , G01N30/46 , G01N30/86
CPC分类号： B01D15/1871 , B01D15/1807 , B01D15/1864 , B01D15/3804 , B01D15/3809 , G01N30/02 , G01N30/26 , G01N30/34 , G01N30/38 , G01N30/44 , G01N30/46 , G01N30/461 , G01N30/462 , G01N30/463 , G01N30/468
摘要： The invention relates to a chromatographic purification method for the isolation of a desired product fraction from a mixture using 2 chromatographic columns, it relates to methods for setting up such a process, and it also relates to control and/or monitoring and/or optimization processes in this context. The method comprises, within one cycle to be carried out at least once, the following steps: a first batch step (Bl), wherein during a batch timespan said columns are disconnected and a first column (1) is loaded with feed via its inlet using a first flow rate and its outlet is directed to waste, and from a second column (2) desired product is recovered via its outlet and subsequently the second column (2) is regenerated; a first interconnected step (IC1), wherein the outlet of the first column (1) is connected to the inlet of the second column (2) during an interconnected timespan, the first column (1) is loaded beyond its dynamic breakthrough capacity with feed via its inlet using a second flow rate which is the same or larger than the first flow rate, and the outlet of the second column (2) is directed to waste, a second batch step (B2) analogous to the first batch step (Bl) but with exchanged columns; a second interconnected step (IC2), analogous to the first interconnected step (IC1) but with exchanged columns.
摘要翻译：本发明涉及一种使用2个色谱柱从混合物中分离所需产物级分的色谱纯化方法，涉及用于建立这种方法的方法，并且还涉及控制和/或监测和/或优化方法在这种情况下该方法包括在一个循环内进行至少一次的步骤：第一批次步骤（B1），其中在批次间隔期间，所述柱断开，并且第一塔（1）通过其入口使用第一流量并且其出口被引导到废物，并且从第二塔（2）通过其出口回收期望的产物，随后再生第二塔（2）; 第一相互连接的步骤（IC1），其中第一列（1）的出口在互连时间段期间连接到第二塔（2）的入口，第一塔（1）被加载超过其动态穿透能力通过其入口使用与第一流量相同或更大的第二流量，并且第二塔（2）的出口被引导废物;第二批次步骤（B2），类似于第一批次步骤（B1 ）但交换列; 第二互连步骤（IC2），类似于第一互连步骤（IC1），但具有交换的列。

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