专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2006138257A3 SINGLE MOLECULE ARRAYS FOR GENETIC AND CHEMICAL ANALYSIS 审中-公开
标题翻译：用于遗传和化学分析的单分子阵列
公开(公告)号：WO2006138257A3
公开(公告)日：2008-12-11
申请号：PCT/US2006022895
申请日：2006-06-13
申请人： CALLIDA GENOMICS INC , DRMANAC RADOJE , CALLOW MATTHEW J , DRMANAC SNEZANA , HAUSER BRIAN K , YEUNG GEORGE
发明人： DRMANAC RADOJE , CALLOW MATTHEW J , DRMANAC SNEZANA , HAUSER BRIAN K , YEUNG GEORGE
IPC分类号： C12Q1/68 , C07H21/04 , C12M1/00 , C12M1/36
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： Random arrays of single molecules are provided for carrying out large scale analyses, particularly of biomolecules, such as genomic DNA, cDNAs, proteins, and the like. In one aspect, arrays of the invention comprise concatemers of DNA fragments that are randomly disposed on a regular array of discrete spaced apart regions, such that substantially all such regions contain no more than a single concatemer. Preferably, such regions have areas substantially less than 1 µm2 and have nearest neighbor distances that permit optical resolution of on the order of 109 single molecules per cm2. Many analytical chemistries can be applied to random arrays of the invention, including sequencing by hybridization chemistries, sequencing by synthesis chemistries, SNP detection chemistries, and the like, to greatly expand the scale and potential applications of such techniques.
摘要翻译：提供单个分子的随机阵列用于进行大规模分析，特别是生物分子，例如基因组DNA，cDNAs，蛋白质等。在一个方面，本发明的阵列包括随机设置在离散间隔开的区域的规则阵列上的DNA片段的并列物质，使得基本上所有这些区域都包含不超过单个并联体。优选地，这样的区域具有基本上小于1μm2的面积，并且具有最近的相邻距离，其允许每厘米每分钟109个单分子的光学分辨率。许多分析化学物质可以应用于本发明的随机阵列，包括杂交化学测序，合成化学测序，SNP检测化学等，以大大扩展这些技术的规模和潜在应用。

2. 发明申请

WO2007044245A3 SELF-ASSEMBLED SINGLE MOLECULE ARRAYS AND USES THEREOF 审中-公开
标题翻译：自组装单分子阵列及其用途
公开(公告)号：WO2007044245A3
公开(公告)日：2009-04-30
申请号：PCT/US2006037938
申请日：2006-09-29
申请人： CALLIDA GENOMICS INC , DRMANAC RADOJE , CALLOW MATTHEW J , HAUSER BRIAN K , YEUNG GEORGE
发明人： DRMANAC RADOJE , CALLOW MATTHEW J , HAUSER BRIAN K , YEUNG GEORGE
IPC分类号： C12Q1/68 , C07H21/04 , C12M1/00 , C12M1/36
CPC分类号： C12Q1/682 , C12Q1/6837 , C12Q2531/125 , C12Q2525/313 , C12Q2525/151
摘要： The present invention provides methods of making and using self-assembled arrays of single polynucleotide molecules for carrying out a variety of large-scale genetic measurements, such as gene expression analysis, gene copy number assessment, and the like. Random arrays used in the invention are "self-assembled" in the sense that they are formed by deposition of polynucleotide molecules onto a surface where they become fixed at random locations. The polynucleotide molecules fixed on the surface are then identified by direct sequence determination of component nucleic acids, such as incorporated probe sequences, or by other decoding schemes. Such identification converts a random array of determinable polynucleotides, and their respective probes into an addressable array of probe sequences.
摘要翻译：本发明提供了制备和使用单个多核苷酸分子的自组装阵列进行各种大规模遗传测量的方法，例如基因表达分析，基因拷贝数评估等。在本发明中使用的随机阵列是“自组装”的意思，即它们通过将多核苷酸分子沉积到其随机位置固定的表面上而形成。然后通过直接测序组分核酸，例如并入的探针序列，或通过其他解码方案来确定表面上固定的多核苷酸分子。这种鉴定将随机阵列的可测定多核苷酸及其各自的探针转换成可寻址的探针序列阵列。

3. 发明申请

WO2006138284A3 NUCLEIC ACID ANALYSIS BY RANDOM MIXTURES OF NON-OVERLAPPING FRAGMENTS 审中-公开
标题翻译：非叠加片段随机混合物的核酸分析
公开(公告)号：WO2006138284A3
公开(公告)日：2007-12-13
申请号：PCT/US2006022950
申请日：2006-06-13
申请人： CALLIDA GENOMICS INC , DRMANAC RADOJE
发明人： DRMANAC RADOJE
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6874 , C07H21/04 , C07K1/047 , C12Q1/6806 , C12Q1/682 , C12Q1/6837 , C12Q1/6869 , C12Q2525/151 , C12Q2525/313 , C12Q2531/125 , C12Q2565/513 , G01N15/1404 , G01N15/1434 , Y10S977/778 , Y10S977/789 , Y10S977/792 , Y10S977/88 , Y10S977/882 , C12Q2521/307 , C12Q2525/161 , C12Q2535/122 , C12Q2563/179 , C12Q2565/514
摘要： The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.
摘要翻译：本发明提供了用于排序从一个或多个目标多核苷酸衍生的序列信息的方法和试剂盒。在一个方面，产生一个或多个分层或等级的碎片和等分试样，之后从最终级别或层级的片段获得序列信息。这样的最后一层中的每个片段都来自特定的等分试样，反过来，这些等分试样来自先前层的特定等分试样，依此类推。对于最后一层中的等分试样的每个片段，从每个先前的层次派生的等分试样是已知的，或者可以被辨别出来。因此，来自不同等分试样的重叠片段的相同序列可以被区分并分组为从与先前层相同或不同的片段衍生的。当最终层中的片段被排序时，使用不同等分试样的片段的重叠序列区域来登记片段，使得非重叠区域被排序。在一个方面，该方法以分级方式进行，直到一个或多个目标多核苷酸被表征为例如。通过其核酸序列，或通过序列片段的排序，或通过单核苷酸多态性（SNP）等的排序。

4. 发明申请

WO2007133831A3 HIGH THROUGHPUT GENOME SEQUENCING ON DNA ARRAYS 审中-公开
标题翻译：在DNA阵列上测序的高通量基因
公开(公告)号：WO2007133831A3
公开(公告)日：2008-02-07
申请号：PCT/US2007062825
申请日：2007-02-26
申请人： COMPLETE GENOMICS INC , DRMANAC RADOJE , CALLOW MATTHEW J , DRMANAC SNEZANA
发明人： DRMANAC RADOJE , CALLOW MATTHEW J , DRMANAC SNEZANA
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6837 , C12Q1/6874 , C12Q2531/125 , C12Q2525/151 , C12Q2521/313
摘要： The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new. e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both "upstream" and "downstream" of the adaptors, identification of entire target sequences may be accomplished.
摘要翻译：本发明涉及使用分散在靶多核苷酸中的衔接子获得靶序列的核苷酸序列信息的方法和组合物。序列信息可以是新的。例如测序未知核酸，重新测序或基因分型。本发明优选地包括在多核苷酸的靶多核苷酸或片段内的间隔位置插入多个衔接子的方法。这样的衔接子可以用作使用各种测序化学品询问相邻序列的平台，例如通过引物延伸，探针连接等鉴定核苷酸的测序化学物质。在本发明中包括用于将已知衔接子序列插入靶序列的方法和组合物，使得与适配器存在连续靶序列的中断。通过对适配器的“上游”和“下游”进行排序，可以完成整个靶序列的识别。

5. 发明申请

WO2008070375A3 SELECTION OF DNA ADAPTOR ORIENTATION 审中-公开
标题翻译：选择DNA适配器方位
公开(公告)号：WO2008070375A3
公开(公告)日：2008-10-02
申请号：PCT/US2007083540
申请日：2007-11-02
申请人： COMPLETE GENOMICS INC , SPARKS ANDREW , OLIPHANT ARNOLD , DRMANAC RADOJE , HUANG STEVEN , YEUNG GEORGE
发明人： SPARKS ANDREW , OLIPHANT ARNOLD , DRMANAC RADOJE , HUANG STEVEN , YEUNG GEORGE
IPC分类号： C40B30/00 , C12Q1/68
CPC分类号： C12N15/10 , C12N15/66
摘要： Aspects described and claimed herein provide methods to insert multiple DNA adaptors into a population of circular target DNAs at defined positions and orientations with respect to one another. The resulting multi-adaptor constructs are then used in massively-parallel nucleic acid sequencing techniques.
摘要翻译：在此描述和要求保护的方面提供了将多个DNA适配器插入到相对于彼此的限定位置和取向的圆形靶DNA群中的方法。然后将所得多连接体构建体用于大规模并行核酸测序技术。

6. 发明申请

WO2012142611A3 SEQUENCING SMALL AMOUNTS OF COMPLEX NUCLEIC ACIDS 审中-公开
标题翻译：序列复合核酸的小数量
公开(公告)号：WO2012142611A3
公开(公告)日：2016-03-03
申请号：PCT/US2012033832
申请日：2012-04-16
申请人： COMPLETE GENOMICS INC , DRMANAC RADOJE , PETERS BROCK A , KERMANI BAHRAM GHAFFARZADEH
发明人： DRMANAC RADOJE , PETERS BROCK A , KERMANI BAHRAM GHAFFARZADEH
IPC分类号： C12Q1/68
CPC分类号： G06F19/22 , C12Q1/6869 , C12Q2537/159 , C12Q2549/10
摘要： The present invention provides methods and compositions for sequencing small amounts of complex nucleic acids (as defined herein) and for analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, among other things. As one example, even after amplifying complex nucleic acids more than 1 000-fold- starting, for example, with genomic DNA from 5- human cells- highly accurate whole genome sequences that are fully haplotyped have been produced.
摘要翻译：本发明提供了用于测序小量复合核酸（如本文所定义）的方法和组合物，并用于分析所得序列信息以减少错误并执行单倍型定相等。作为一个示例，即使在扩增了多于1000倍的复合核酸之后，例如，使用来自5个人细胞的基因组DNA，已经产生了完全单体型的高度准确的全基因组序列。

7. 发明申请

WO2012142531A3 PROCESSING AND ANALYSIS OF COMPLEX NUCLEIC ACID SEQUENCE DATA 审中-公开
标题翻译：复杂核酸序列数据的处理和分析
公开(公告)号：WO2012142531A3
公开(公告)日：2014-05-08
申请号：PCT/US2012033686
申请日：2012-04-13
申请人： COMPLETE GENOMICS INC , DRMANAC RADOJE , PETERS BROCK A , KERMANI BAHRAM GHAFFARZADEH
发明人： DRMANAC RADOJE , PETERS BROCK A , KERMANI BAHRAM GHAFFARZADEH
IPC分类号： G06F19/10
CPC分类号： G06F19/22
摘要： The present invention is directed to logic for analysis of nucleic acid sequence data that employs algorithms that lead to a substantial improvement in sequence accuracy and that can be used to phase sequence variations, e.g., in connection with the use of the long fragment read (LFR) process.
摘要翻译：本发明涉及用于分析核酸序列数据的逻辑，其使用导致序列精度的实质性改进并且可用于相序变化的算法，例如结合使用长片段读取（LFR ）过程。

8. 发明申请

WO0164834A8 NOVEL NUCLEIC ACIDS AND POLYPEPTIDES 审中-公开
标题翻译：新型核酸和多肽
公开(公告)号：WO0164834A8
公开(公告)日：2002-02-14
申请号：PCT/US0104926
申请日：2001-02-26
申请人： HYSEQ INC , TANG Y TOM , LIU CHENGHUA , ZHOU PING , ASUNDI VINOD , ZHANG JIE , ZHAO QING A , REN FEIYAN , XUE AIDONG J , YANG YONGHONG , WEHRMAN TOM , WANG JIAN RUI , MA YUNQING , WANG DUNRUI , CHEN RUI HONG , XU CHONGJUN , DRMANAC RADOJE
发明人： TANG Y TOM , LIU CHENGHUA , ZHOU PING , ASUNDI VINOD , ZHANG JIE , ZHAO QING A , REN FEIYAN , XUE AIDONG J , YANG YONGHONG , WEHRMAN TOM , WANG JIAN-RUI , MA YUNQING , WANG DUNRUI , CHEN RUI-HONG , XU CHONGJUN , DRMANAC RADOJE
IPC分类号： C07K14/47 , C12Q1/68 , C07H21/04 , C07K14/435
CPC分类号： C07K14/47 , C12Q1/6886
摘要： La présente invention concerne de nouveaux acides nucléiques, de nouvelles séquences polypeptidiques codées par lesdits acides nucléiques et leurs utilisations.
摘要翻译：本发明涉及新型核酸，由所述核酸编码的新多肽序列及其用途。

9. 发明申请

WO2009073629A2 EFFICIENT SHOTGUN SEQUENCING METHODS 审中-公开
标题翻译：有效的SHOTGUN测序方法
公开(公告)号：WO2009073629A2
公开(公告)日：2009-06-11
申请号：PCT/US2008085189
申请日：2008-12-01
申请人： COMPLETE GENOMICS INC , DRMANAC RADOJE , REID CLIFFORD
发明人： DRMANAC RADOJE , REID CLIFFORD
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2549/119 , C12Q2537/149 , C12Q2565/137
摘要： Methods are provided for efficient shotgun sequencing to allow efficient selection and sequencing of nucleic acids of interest contained in a library. The nucleic acids of interest can be defined any time before or after preparation of the library. One example of nucleic acids of interest is missing or low confidence genome sequences resulting from an initial sequencing procedure. Other nucleic acids of interest include subsets of genomic DNA, RNA or cDNAs (exons, genes, gene sets, transciptomes). By designing an efficient (simple to implement, speedy, high specificity, low cost) selection procedure, a more complete sequence is achieved with less effort than by using highly redundant shotgun sequencing in an initial sequencing procedure
摘要翻译：提供了用于有效鸟枪法测序的方法，以允许对库中所含目的核酸进行有效选择和测序。感兴趣的核酸可以在文库制备之前或之后的任何时候定义。感兴趣的核酸的一个例子是由初始测序程序产生的缺失或低置信度的基因组序列。其他感兴趣的核酸包括基因组DNA，RNA或cDNA（外显子，基因，基因组，转座体）的亚组。通过设计一个有效的（简单实施，快速，高特异性，低成本）选择程序，以比在初始测序程序中使用高度冗余鸟枪法测序更少的努力实现更完整的序列

10. 发明申请

WO0153456A2 METHODS AND MATERIALS RELATING TO PROTHROMBINASE-LIKE POLYPEPTIDES AND POLYNUCLEOTIDES 审中-公开
标题翻译：与原丙氨酸样多肽和多核苷酸相关的方法和材料
公开(公告)号：WO0153456A2
公开(公告)日：2001-07-26
申请号：PCT/US0035061
申请日：2000-12-22
申请人： HYSEQ INC , HALEY DANA A , BOYLE BRYAN J , SUK YUE HO ALICE , ZHOU PING , MIZE NANCY K , KUO CHIAUYUN , ARTERBURN MATTHEW C , TANG Y TOM , LIU CHENGHUA , DRMANAC RADOJE
发明人： HALEY DANA A , BOYLE BRYAN J , SUK-YUE HO ALICE , ZHOU PING , MIZE NANCY K , KUO CHIAUYUN , ARTERBURN MATTHEW C , TANG Y TOM , LIU CHENGHUA , DRMANAC RADOJE
IPC分类号： G01N33/50 , A61K35/76 , A61K38/00 , A61K38/43 , A61K45/00 , A61K48/00 , A61P3/02 , A61P3/10 , A61P13/12 , A61P19/02 , A61P21/00 , A61P25/00 , A61P25/16 , A61P25/28 , A61P29/00 , A61P31/00 , A61P31/04 , A61P35/00 , A61P37/00 , C07H21/02 , C07H21/04 , C07K14/00 , C07K14/475 , C07K14/705 , C07K14/745 , C07K16/36 , C12M1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/16 , C12N9/64 , C12N15/00 , C12N15/09 , C12N15/12 , C12N15/63 , C12N15/85 , C12N15/86 , C12P19/34 , C12P21/02 , C12Q1/02 , C12Q1/68 , G01N33/15 , G01N33/53 , G01N33/566 , C12N
CPC分类号： C12N9/6432 , A61K38/00 , C07K14/70503 , C12N9/16 , C12Y304/21006
摘要： The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel secreted prothrombinase-like polypeptide. These polynucleotides comprise nucleic acid sequences isolated from cDNA library from human ovary (Hyseq clone identification numbers 773565 (SEQ ID NO: 1) and 7727065 (SEQ ID NO: 11)). Other aspects of the invention include vectors containing processes for producing novel secreted prothrombinase-like polypeptides, and antibodies specific for such polypeptides.
摘要翻译：本发明提供由对应于新分泌的凝血酶原酶样多肽的这种多核苷酸及其突变体或变体编码的新型多核苷酸和多肽。这些多核苷酸包含从人卵巢的cDNA文库（Hyseq克隆鉴定号773565（SEQ ID NO：1）和7727065（SEQ ID NO：11））分离的核酸序列。本发明的其它方面包括含有产生新型分泌的凝血酶原酶样多肽的方法的载体和对这些多肽具有特异性的抗体。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式