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1. 发明申请

WO2006076046A3 BRANCHED AND MULTI-CHAIN NUCLEIC ACID SWITCHES FOR SENSING AND SCREENING 审中-公开
标题翻译：用于检测和筛选的分支和多链核酸开关
公开(公告)号：WO2006076046A3
公开(公告)日：2007-02-15
申请号：PCT/US2005027527
申请日：2005-08-02
申请人： UNIV SYRACUSE , BORER PHILIP N , HUDSON BRUCE S
发明人： BORER PHILIP N , HUDSON BRUCE S
IPC分类号： C12Q1/68
CPC分类号： C12N15/115 , C12N2310/16 , C12N2310/3517 , C12Q1/6818 , C12Q1/70 , C12Q1/701 , C12Q2541/101 , C12Q2525/313 , C12Q2525/205 , C12Q2525/301 , C12Q2565/301
摘要： Embodiments of the invention relate to a branched or multichain nucleic acid switch adapted to switch from a first conformation to a second conformation upon ligand binding. The switch includes a probe strand, P, which includes the ligand binding domain; a switching framework which includes a cover strand (C), and a tether that holds P and C together and a signaling apparatus. Some embodiments include a toggle strand (T) where now the tether holds P, C, T, and the signaling apparatus together. As the switch changes between the first and second conformations; the signaling apparatus reports the state of the switch. The signaling entity is typically a lumiphore and a quencher located along the switching framework. Nucleic acid switches have applications in real time assays for diverse agents including infectious agents, environmental toxins, and terrorist agents, as well as screening' methods for such agents. Further applications are found for nanoelectronics, nanofabrication and nanomachines.
摘要翻译：本发明的实施方案涉及适合于在配体结合后从第一构象切换至第二构象的分枝或多核酸核酸开关。该开关包括探针链P，其包含配体结合结构域; 包括盖股（C）和将P和C保持在一起的系绳和信号装置的开关框架。一些实施例包括肘节线束（T），其中绳索现在将P，C，T和信号装置保持在一起。随着开关在第一种和第二种构象之间变化，信令装置报告交换机的状态。信令实体通常是沿交换框架定位的发光体和猝灭剂。核酸开关可用于实时分析各种物质，包括传染物，环境毒素和恐怖分子，以及筛选这些物质的方法。纳米电子学，纳米加工和纳米机器还有其他应用。

2. 发明申请

WO2004069850A2 SWITCHABLE NUCLEIC ACIDS FOR DIAGNOSTICS, SCREENING AND MOLECULAR ELECTRONICS 审中-公开
标题翻译：用于诊断，筛选和分子电子的可切换核酸
公开(公告)号：WO2004069850A2
公开(公告)日：2004-08-19
申请号：PCT/US2004003002
申请日：2004-02-03
申请人： BORER PHILIP N , HUDSON BRUCE S
发明人： BORER PHILIP N , HUDSON BRUCE S
IPC分类号： C07H21/00 , C12N15/63 , C12Q1/68 , C07H
CPC分类号： C12Q1/68 , C07H21/00 , C12N15/63 , C12N15/635 , C12Q1/6818 , C12Q2565/133 , C12Q2525/205 , C12Q2525/301
摘要： In one embodiment, the present invention relates to fluorescent nucleic acid constructs and methods of using these switchable constructs to rapidly screen for target molecule interactions. More particularly, an RNA/DNA chimera comprising a fluorophore-quencher pair and a nucleic acid construct is disclosed for the rapid screening of interactions between the HIV-1 nucleocapsid protein, NCp7, and a stem-loop region, SL3, of the HIV-1 RNA, or antagonists thereof. The compositions and methods disclosed herein can be used in preferred aspects of the present invention for diagnosing disease states, distinguishing the presence of infectious or toxic agents, drug discovery and design, and molecular electronic applications.
摘要翻译：在一个实施方案中，本发明涉及使用这些可切换构建体快速筛选靶分子相互作用的荧光核酸构建体和方法。更具体地，公开了包含荧光团 - 猝灭剂对和核酸构建体的RNA / DNA嵌合体，用于快速筛选HIV-1核衣壳蛋白NCp7和HIV-1核衣壳蛋白的茎环区域SL3之间的相互作用， 1 RNA或其拮抗剂。本文公开的组合物和方法可用于本发明的优选方面，用于诊断疾病状态，区分感染性或毒性剂的存在，药物发现和设计以及分子电子应用。

3. 发明申请

WO2006076046A9 BRANCHED AND MULTI-CHAIN NUCLEIC ACID SWITCHES FOR SENSING AND SCREENING 审中-公开
标题翻译：用于感测和筛选的分支和多链式核酸开关
公开(公告)号：WO2006076046A9
公开(公告)日：2007-04-05
申请号：PCT/US2005027527
申请日：2005-08-02
申请人： UNIV SYRACUSE , BORER PHILIP N , HUDSON BRUCE S
发明人： BORER PHILIP N , HUDSON BRUCE S
IPC分类号： C12Q1/68
CPC分类号： C12N15/115 , C12N2310/16 , C12N2310/3517 , C12Q1/6818 , C12Q1/70 , C12Q1/701 , C12Q2541/101 , C12Q2525/313 , C12Q2525/205 , C12Q2525/301 , C12Q2565/301
摘要： Embodiments of the invention relate to a branched or multichain nucleic acid switch adapted to switch from a first conformation to a second conformation upon ligand binding. The switch includes a probe strand, P, which includes the ligand binding domain; a switching framework which includes a cover strand (C), and a tether that holds P and C together and a signaling apparatus. Some embodiments include a toggle strand (T) where now the tether holds P, C, T, and the signaling apparatus together. As the switch changes between the first and second conformations; the signaling apparatus reports the state of the switch. The signaling entity is typically a lumiphore and a quencher located along the switching framework. Nucleic acid switches have applications in real time assays for diverse agents including infectious agents, environmental toxins, and terrorist agents, as well as screening' methods for such agents. Further applications are found for nanoelectronics, nanofabrication and nanomachines.
摘要翻译：本发明的实施方案涉及一种支链或多链核酸开关，其适于在配体结合时从第一构象切换到第二构象。该开关包括探针链，其包括配体结合结构域; 包括盖链（C）和将P和C保持在一起的系链和信号装置的切换框架。一些实施例包括切换链（T），其中系绳将P，C，T和信令装置保持在一起。当开关在第一和第二构象之间变化时; 信令装置报告交换机的状态。信令实体通常是沿着交换框架定位的集线器和猝熄器。核酸开关可用于多种试剂的实时测定，包括感染因子，环境毒素和恐怖分子，以及筛选这些药剂的方法。发现纳米电子学，纳米制造和纳米机械的其他应用。

4. 发明申请

WO2006102540A3 ELUCIDATION OF HIGH AFFINITY, HIGH SPECIFICITY OLIGONUCLEOTIDES AND DERIVATIZED OLIGONUCLEOTIDE SEQUENCES FOR TARGET RECOGNITION 审中-公开
标题翻译：高度亲和力，高特异性寡核苷酸和衍生化寡核苷酸序列的靶向鉴定
公开(公告)号：WO2006102540A3
公开(公告)日：2007-01-04
申请号：PCT/US2006010645
申请日：2006-03-23
申请人： UNIV SYRACUSE , HUDSON BRUCE S , BORER PHILIP N
发明人： HUDSON BRUCE S , BORER PHILIP N
IPC分类号： C07H21/02 , C07H21/04
CPC分类号： G01N33/5308 , C12Q1/6837 , G01N2333/44
摘要： A method is disclosed to obtain oligonucleotide sequences with high affinity to target molecules. By design, the oligonucleotides have a defined primary and secondary structure. The affinity for binding to target species is classified or quantified by assay measurements using physical measurements rather than being based primarily on separations. Targets include but are not limited to proteins, polymers, biological membranes including cells and organelles and small molecules.
摘要翻译：公开了获得对目标分子具有高亲和力的寡核苷酸序列的方法。通过设计，寡核苷酸具有确定的一级和二级结构。通过使用物理测量的测定测量来分类或量化与靶物种结合的亲和力，而不是主要基于分离。目标包括但不限于蛋白质，聚合物，生物膜，包括细胞和细胞器以及小分子。

5. 发明申请

WO2009151688A3 DIRECT SELECTION OF STRUCTURALLY DEFINED APTAMERS 审中-公开
标题翻译：直接选择结构定义的APTAMERS
公开(公告)号：WO2009151688A3
公开(公告)日：2010-03-04
申请号：PCT/US2009037022
申请日：2009-03-12
申请人： UNIV SYRACUSE , BORER PHILIP N , MCPIKE MARK P
发明人： BORER PHILIP N , MCPIKE MARK P
IPC分类号： C12N15/115 , C12Q1/68
CPC分类号： C12N15/115 , C12N15/1048 , C12N15/111 , C12N2310/16 , C12N2320/13 , C12N2330/31 , C12Q1/6811 , C12Q2565/518 , C12Q2525/205 , C12Q2525/151
摘要： The present invention provides aptamer libraries with pre-defined secondary structures that can be used for oversampled screening for affinity binding. In one embodiment, a multiplex approach is employed to divide the library into degenerate subsets that are immobilized on multiple locations of a solid support such as a microarray chip
摘要翻译：本发明提供具有预定义的二级结构的适体文库，其可用于亲和结合的过取样筛选。在一个实施方案中，使用多重方法将文库分解为固定在固体支持物的多个位置（例如微阵列芯片）上的简并子集

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