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1. 发明申请

WO2005114191A2 METHOD FOR A FULLY AUTOMATED MONOCLONAL ANTIBODY-BASED EXTENDED DIFFERENTIAL 审中-公开
标题翻译：全自动单核抗体基于扩增差异的方法
公开(公告)号：WO2005114191A2
公开(公告)日：2005-12-01
申请号：PCT/US2005/017996
申请日：2005-05-18
申请人： BECKMAN COULTER, INC.
发明人： PAUL, Ronald, D. , WYATT, James, L. , CARRILLO, Barbara , RUBIO, Oilda , CAREAGA, Diana, B. , LOPEZ, Lidice, L.
IPC分类号： G01N33/53
CPC分类号： G01N33/56966 , G01N15/1459 , G01N33/56972 , G01N2015/1006 , G01N2015/1402 , Y10T436/101666 , Y10T436/107497 , Y10T436/13 , Y10T436/25125
摘要： A method useful for the enumeration of cell populations in a biological sample includes the steps of reacting in a single reaction mixture a sample, a first antibody labeled with a fluorochrome having a first emission spectrum and an additional antibody. The first antibody binds to an antigenic determinant differentially expressed on leukocytes and non-leukocytes. The additional antibody binds to an antigenic determinant differentially expressed on mature and immature granulocytes or myeloid cells, and is labeled either with the first fluorochrome or an additional fluorochrome having an emission spectrum distinguishable from the first emission spectrum. The reaction mixture can be mixed with a nucleic acid dye having an emission spectrum that overlaps with one of the first or additional emission spectra. The reaction mixture may be treated with a lytic system that differentially lyses non-nucleated red blood cells and conserves leukocytes. Populations of hematological cells are detected and enumerated using at least two parameters (fluorescence, optical, and electrical) for each population.
摘要翻译：可用于计数生物样品中细胞群体的方法包括以下步骤：在单一反应混合物中使样品，用具有第一发射光谱的荧光染料标记的第一抗体和另外的抗体反应。第一抗体结合在白细胞和非白细胞上差异表达的抗原决定簇。另外的抗体结合在成熟和未成熟粒细胞或骨髓细胞上差异表达的抗原决定簇，并且用第一荧光染料或具有与第一发射光谱可区分的发射光谱的附加荧光染料进行标记。反应混合物可以与具有与第一或另外的发射光谱之一重叠的发射光谱的核酸染料混合。反应混合物可以用裂解体系来处理，所述裂解系统差异地裂解非成核红细胞并保存白细胞。使用每个群体的至少两个参数（荧光，光学和电）检测和计数血细胞的群体。

2. 发明申请

WO2004085989A3 DYE COMPOSITIONS WHICH PROVIDE ENHANCED DIFFERENTIAL FLUORESCENCE AND LIGHT SCATTER CHARACTERISTICS 审中-公开
公开(公告)号：WO2004085989A3
公开(公告)日：2004-10-07
申请号：PCT/US2004/006889
申请日：2004-03-05
申请人： BECKMAN COULTER, INC.
发明人： MAPLES, John, A. , LOPEZ, Lidice, L. , TORKE, Nancy
IPC分类号： C12Q1/68
摘要： A composition for enhancing differential staining of RNA, DNA and granules in a sample comprising cells contains a first fluorescent dye that can bind specific binding sites and non-specific binding sites in the sample. This first dye emits fluorescence at a first wavelength. The composition contains at least an additional component, which is a second non-intercalating dye in the composition that competes with said first dye for binding to the nonspecific binding sites, or a permeabilizing agent to enhance permabilization of the dyes into the cells, or both. The molar ratio of the second dye and the first dye is at least about 20:1.

3. 发明申请

WO2008002745A3 METHOD FOR A FULLY AUTOMATED MONOCLONAL ANTIBODY-BASED EXTENDED DIFFERENTIAL 审中-公开
公开(公告)号：WO2008002745A3
公开(公告)日：2008-01-03
申请号：PCT/US2007/070301
申请日：2007-06-04
申请人： BECKMAN COULTER, INC.
发明人： PAUL, Ronald, D. , RUBIO, Olida , CAREAGA, Diana, B. , LOPEZ, Lidice, L. , MYLVAGANAM, Ravindra
IPC分类号： G01N33/53
摘要： Methods for differentially identifying cells in an instrument employ compositions containing a combination of selected antibodies and fluorescent dyes having different cellular distribution patterns and specificities, as well as antibodies and fluorescent dyes characterized by overlapping emission spectra which form non-compensatable spectral patterns. When utilizing the compositions described herein consisting of fluorescent dyes and fluorochrome labeled antibodies with overlapping spectra that cannot be separated or distinguished based upon optical or electronic compensation means, a new fluorescent footprint is established. This new fluorescent footprint is a result of the overlapping spectra and the combined cellular staining patterns of the dyes and fluorochrome labeled antibodies chosen for the composition. The new fluorescent footprint results in histogram patterns that are useful for the identification of additional cell populations or subtypes in hematological disease.

4. 发明申请

WO2007056622A3 METHOD FOR DISCRIMINATING PLATELETS FROM RED BLOOD CELLS 审中-公开
公开(公告)号：WO2007056622A3
公开(公告)日：2007-05-18
申请号：PCT/US2006/060024
申请日：2006-10-17
申请人： BECKMAN COULTER, INC.
发明人： PEREZ, Carlos, A. , LOPEZ, Lidice, L. , WELLS, Mark, A. , IBANEZ, Joaquin , LANDRUM, Eileen , DEL VALLE, Roberto , GALVEZ, Santiago
IPC分类号： G01N21/00
摘要： A method for discriminating and quantifying platelets within an analyzed blood sample involves initially diluting the blood sample with a ghosting reagent that causes a change in the index of refraction of the cell. Owing to the change in the index of refraction, light scattered from the ghosted red blood cells will be substantially reduced relative to light scattered from platelets. This results in locations of platelets within a scatterplot of the analyzed blood sample to fall within a region distinguishable from those containing normal red blood cells, fragmented red blood cells, and microcytic red blood cells.

5. 发明申请

WO2007087094A2 METHOD OF MEASUREMENT OF NUCLEATED RED BLOOD CELLS 审中-公开
标题翻译：核红细胞测量方法
公开(公告)号：WO2007087094A2
公开(公告)日：2007-08-02
申请号：PCT/US2006/061847
申请日：2006-12-11
申请人： BECKMAN COULTER, INC.
发明人： LOPEZ, Lidice, L. , PEREZ, Carlos, A. , WELLS, Mark, A. , CAREAGA, Diana, B. , HUO, Ziling , QIAN, Cheng , TORKE, Nancy, J.
IPC分类号： G01N33/50
CPC分类号： G01N33/80 , G01N15/1031 , G01N15/12 , G01N15/1459 , G01N33/5094 , G01N2015/0073 , G01N2015/008 , G01N2015/1037 , G01N2015/1062 , G01N2015/1486 , Y10T436/101666 , Y10T436/25125
摘要： Methods are provided for measurement of nucleated red blood cells in a blood sample. The methods include exposing a blood cell sample to a reagent system to lyse mature red blood cells, subsequently analyzing nucleated red blood cells in a flow cell by axial light loss, DC impedance, and medium angle light scatter measurements; by axial light loss, low angle light scatter, and medium angle light scatter measurements; or by axial light loss, DC impedance, low angle light scatter, and medium angle light scatter measurements; and then differentiating nucleated red blood cells from other cell types by using measured signals and/or functions thereof.
摘要翻译：提供了用于测量血液样品中有核红细胞的方法。所述方法包括将血细胞样品暴露于试剂系统以裂解成熟红细胞，随后通过轴向光损失，DC阻抗和中等角度光散射测量分析流动池中的有核红细胞; 通过轴向光损失，低角度光散射和中等角度光散射测量; 或通过轴向光损失，直流阻抗，低角度光散射和中等角度光散射测量; 然后通过使用测量的信号和/或功能将有核红细胞与其它细胞类型区分开来。

6. 发明申请

WO2005076995A3 METHOD OF MEASUREMENT OF NUCLEATED RED BLOOD CELLS 审中-公开
公开(公告)号：WO2005076995A3
公开(公告)日：2005-08-25
申请号：PCT/US2005/003853
申请日：2005-02-01
申请人： BECKMAN COULTER, INC.
发明人： LOPEZ, Lidice, L. , PEREZ, Carlos, A. , WELLS, Mark, A. , CAREAGA, Diana, B. , HUO, Ziling , QIAN, Cheng
IPC分类号： G01N33/48
摘要： Methods are provided for measurement of nucleated red blood cells. The methods include exposing a blood cell sample to a reagent system to lyse mature red blood cells, subsequently analyzing nucleated red blood cells in a flow cell by axial light loss, low angle light scatter and DC impedance measurements, or two selected measurements thereof; and then differentiating nucleated red blood cells from other cell types by using measured signals and/or functions thereof.

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