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    • 4. 发明申请
    • SYSTEM AND METHOD FOR PLANAR VOLTAGE-CLAMP ELECTROPHYSIOLOGICAL MEASUREMENTS ON MULTIPLE CELLS AND THEIR USE IN IDENTIFYING AGENTS THAT MODULATE ION CHANNELS
    • 用于平面电压钳位电泳测量多个细胞的系统和方法及其在识别离子通道的鉴定试剂中的应用
    • WO2005080971A1
    • 2005-09-01
    • PCT/SE2005/000239
    • 2005-02-21
    • ASTRAZENECA ABDAVIS, AndrewHARGREAVES, AndrewHENTHORN, DeborahORME, Alexandra
    • DAVIS, AndrewHARGREAVES, AndrewHENTHORN, DeborahORME, Alexandra
    • G01N33/487
    • G01N33/48728
    • The present invention relates to an assay method for identifying an agent that modulates the electrical property of a membranous sample comprising the steps of: (a) providing an electrophysiological measurement apparatus; (b) contacting one or more membranous samples with a compartment of the electrophysiological measurement apparatus containing an aperture; (c) forming a seal between the membranous samples and the aperture in the presence of a low ion concentration seal phase internal solution; (d) exchanging the low ion concentration seal phase internal solution for an access phase internal solution; (e) establishing electrical access, via the aperture, to the internal compartment of the membranous sample; and (f) taking electrophysiological recordings before and after incubation of the cell with one or more agents; wherein a difference between: (i) the electrophysiological recording in the presence of the agent; and (ii) the electrophysiological recording in the absence of the agent is indicative of an agent that modulates the electrical property of a membranous sample.
    • 本发明涉及一种用于鉴定调节膜样品的电性质的试剂的测定方法,包括以下步骤:(a)提供电生理测量装置; (b)使一个或多个膜样品与包含孔的电生理测量装置的隔室接触; (c)在低离子浓度密封相内溶液存在下,在膜样品和孔之间形成密封; (d)交换低离子浓度密封相内部溶液用于进入相内部溶液; (e)通过孔径建立到膜样品的内部隔室的电气通路; 和(f)在细胞与一种或多种药剂孵育之前和之后进行电生理记录; 其特征在于:(i)所述药剂存在下的电生理记录; 和(ii)在没有药剂的情况下的电生理记录是指调节膜样品的电性能的试剂。