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1. 发明申请

WO0134821A2 CHEMICAL INDUCIBLE PROMOTER USED TO OBTAIN TRANSGENIC PLANTS WITH A SILENT MARKER AND ORGANISMS AND CELLS AND METHODS OF USING SAME FOR SCREENING FOR MUTATIONS 审中-公开
标题翻译：用于获得具有无机标记和有机体和细胞的转基因植物的化学诱导促进剂及其筛选用于筛选的方法
公开(公告)号：WO0134821A2
公开(公告)日：2001-05-17
申请号：PCT/US0031070
申请日：2000-11-13
申请人： UNIV ROCKEFELLER , ZUO JIANRU , CHUA NAM HAI
发明人： ZUO JIANRU , CHUA NAM-HAI
IPC分类号： A01H1/00 , C12N5/10 , C12N15/09 , C12N15/82 , A01H5/00 , C12Q1/02 , C12Q1/68
CPC分类号： C12N15/8274 , C12N15/8209 , C12N15/8237 , C12N15/8238 , C12N15/8242 , C12N15/8295
摘要： Disclosed is a chemically inducible promoter for transforming plants or plant cells with genes which are regulatable by adding the plants or cells to a medium containing an inducer or by removing them from such medium. The promoter is inducible by a glucocorticoid, estrogen or inducer not endogenous to plants. Such promoters may be used with any plant genes that can promote shoot regeneration and development to induce shoot formation in the presence of a glucocorticoid, estrogen or inducer. The promoter may be used with antibiotic or herbicide resistance genes or other genes which are regulatable by the presence or absence of a given inducer. Also presented are organisms or cells comprising a gene wherein the natural promoter of the gene is disrupted and the gene is placed under the control of a transgenic inducible promoter. These organisms and cells and their progeny are useful for screening for conditional gain of function and loss of function mutations.
摘要翻译：公开了一种化学诱导型启动子，用于通过将植物或细胞添加到含有诱导物的培养基中或通过从这种培养基中除去可调节基因来转化植物或植物细胞。启动子可通过糖皮质激素，雌激素或对植物无内源性的诱导物诱导。这样的启动子可以与任何植物基因一起使用，所述植物基因可以在糖皮质激素，雌激素或诱导物的存在下促进芽再生和发育以诱导芽形成。启动子可以与抗生素或除草剂抗性基因或其它可通过给定诱导物的存在或不存在而调节的基因一起使用。还提供了包含基因的生物体或细胞，其中基因的天然启动子被破坏并且将基因置于转基因诱导型启动子的控制下。这些生物和细胞及其后代可用于筛选功能条件增加和功能突变丧失。

2. 发明申请

WO2003037072A2 PROMOTION OF SOMATIC EMBRYOGENESIS IN PLANTS BY WUSCHEL GENE EXPRESSION 审中-公开
标题翻译：通过WUSCHEL基因表达促进植物中的生物发育
公开(公告)号：WO2003037072A2
公开(公告)日：2003-05-08
申请号：PCT/US2002/034534
申请日：2002-10-28
申请人： THE ROCKEFELLER UNIVERSITY , ZUO, Jianru , NIU, Qi-Wen , FRUGIS, Giovanna , CHUA, Nam-Hai
发明人： ZUO, Jianru , NIU, Qi-Wen , FRUGIS, Giovanna , CHUA, Nam-Hai
IPC分类号： A01H
CPC分类号： C12N15/8261 , A01H4/005 , C12N15/821 , C12N15/8287 , Y02A40/146
摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.
摘要翻译：本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。在另一个实施方案中，这种表达可以用于赋予植物无义变异体。在另一个实施方案中，这种过表达可用于产生可用于产生双倍体植物的单倍体植物。

3. 发明申请

WO03037072A3 PROMOTION OF SOMATIC EMBRYOGENESIS IN PLANTS BY WUSCHEL GENE EXPRESSION 审中-公开
标题翻译： WUSCHEL基因表达对植物体细胞胚胎发生的促进作用
公开(公告)号：WO03037072A3
公开(公告)日：2003-10-16
申请号：PCT/US0234534
申请日：2002-10-28
申请人： UNIV ROCKEFELLER , ZUO JIANRU , NIU QI-WEN , FRUGIS GIOVANNA , CHUA NAM-HAI
发明人： ZUO JIANRU , NIU QI-WEN , FRUGIS GIOVANNA , CHUA NAM-HAI
IPC分类号： C12N15/82 , C12N5/04 , A01H1/00 , A01H4/00 , A01H5/00 , C12N5/10
CPC分类号： C12N15/8261 , A01H4/005 , C12N15/821 , C12N15/8287 , Y02A40/146
摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.
摘要翻译：本发明涉及通过在所述组织或器官中过表达Wuschel基因从植物的组织或器官促进体细胞胚发生的方法。在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。在另一个实施方案中，这种表达可用于赋予植物无融合生殖。在另一个实施方案中，可以使用这种过表达来产生可用于生产双倍体植物的单倍体植物。

4. 发明申请

WO0134821A9 CHEMICAL INDUCIBLE PROMOTER USED TO OBTAIN TRANSGENIC PLANTS WITH A SILENT MARKER AND ORGANISMS AND CELLS AND METHODS OF USING SAME FOR SCREENING FOR MUTATIONS 审中-公开
标题翻译：用于获得具有无机标记和有机体和细胞的转基因植物的化学诱导促进剂及其筛选用于筛选的方法
公开(公告)号：WO0134821A9
公开(公告)日：2002-06-27
申请号：PCT/US0031070
申请日：2000-11-13
申请人： UNIV ROCKEFELLER , ZUO JIANRU , CHUA NAM-HAI
发明人： ZUO JIANRU , CHUA NAM-HAI
IPC分类号： A01H1/00 , C12N5/10 , C12N15/09 , C12N15/82 , A01H5/00 , C12Q1/02 , C12Q1/68
CPC分类号： C12N15/8274 , C12N15/8209 , C12N15/8237 , C12N15/8238 , C12N15/8242 , C12N15/8295
摘要： Disclosed is a chemically inducible promoter for transforming plants or plant cells with genes which are regulatable by adding the plants or cells to a medium containing an inducer or by removing them from such medium. The promoter is inducible by a glucocorticoid, estrogen or inducer not endogenous to plants. Such promoters may be used with any plant genes that can promote shoot regeneration and development to induce shoot formation in the presence of a glucocorticoid, estrogen or inducer. The promoter may be used with antibiotic or herbicide resistance genes or other genes which are regulatable by the presence or absence of a given inducer. Also presented are organisms or cells comprising a gene wherein the natural promoter of the gene is disrupted and the gene is placed under the control of a transgenic inducible promoter. These organisms and cells and their progeny are useful for screening for conditional gain of function and loss of function mutations.
摘要翻译：公开了一种化学诱导型启动子，用于通过将植物或细胞添加到含有诱导物的培养基中或通过从这种培养基中除去可调节基因来转化植物或植物细胞。启动子可通过糖皮质激素，雌激素或对植物无内源性的诱导物诱导。这样的启动子可以与任何植物基因一起使用，所述植物基因可以在糖皮质激素，雌激素或诱导物的存在下促进芽再生和发育以诱导芽形成。启动子可以与抗生素或除草剂抗性基因或其它可通过给定诱导物的存在或不存在而调节的基因一起使用。还提供了包含基因的生物体或细胞，其中基因的天然启动子被破坏并且将基因置于转基因诱导型启动子的控制下。这些生物和细胞及其后代可用于筛选功能条件增加和功能突变丧失。

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