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1. 发明申请

WO2007094762A3 NOVEL PLANT HOMEODOMAIN PROTEIN-ENCODING GENES AND THEIR USES 审中-公开
公开(公告)号：WO2007094762A3
公开(公告)日：2007-08-23
申请号：PCT/US2006/004777
申请日：2006-02-13
申请人： TEMASEK LIFE SCIENCES LABORATORY , JI, Lianghui , CAI, Lin , CHUA, Nam-Hai
发明人： JI, Lianghui , CAI, Lin , CHUA, Nam-Hai
IPC分类号： C12N15/82 , C12N15/00 , A01H5/00 , A01H1/00 , C07H21/04
摘要： A group of genes including GhCIR1 from cotton ( Gossypium hirsutum ), and AtCIR1 and AtCIR2 from Arabidopsis thaliana promote shoot regeneration in plants even in the absence of cytokinin. In the presence of cytokinin, the genes significantly improve transformation efficiency. The genes can be used as an enhancer as well as a selectable marker of transformation in plants. The proteins encoded by the novel genes have a homeodomain (HD) at the N-terminus and a highly divergent domain at the C-terminus. The proteins share a common structural motif.

2. 发明申请

WO2007094762A2 NOVEL PLANT HOMEODOMAIN PROTEIN-ENCODING GENES AND THEIR USES 审中-公开
标题翻译：新的植物家庭蛋白质编码基因及其用途
公开(公告)号：WO2007094762A2
公开(公告)日：2007-08-23
申请号：PCT/US2006004777
申请日：2006-02-13
申请人： TEMASEK LIFE SCIENCES LAB , JI LIANGHUI , CAI LIN , CHUA NAM-HAI
发明人： JI LIANGHUI , CAI LIN , CHUA NAM-HAI
CPC分类号： C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/821 , C12N15/8261 , Y02A40/146
摘要： A group of genes including GhCIR1 from cotton (Gossypium hirsutum), and AtCIR1 and AtCIR2 from Arabidopsis
摘要翻译：包括来自棉花（Gossypium hirsutum）的GhCIR1和来自拟南芥的AtCIR1和AtCIR2的一组基因

3. 发明申请

WO0134821A2 CHEMICAL INDUCIBLE PROMOTER USED TO OBTAIN TRANSGENIC PLANTS WITH A SILENT MARKER AND ORGANISMS AND CELLS AND METHODS OF USING SAME FOR SCREENING FOR MUTATIONS 审中-公开
标题翻译：用于获得具有无机标记和有机体和细胞的转基因植物的化学诱导促进剂及其筛选用于筛选的方法
公开(公告)号：WO0134821A2
公开(公告)日：2001-05-17
申请号：PCT/US0031070
申请日：2000-11-13
申请人： UNIV ROCKEFELLER , ZUO JIANRU , CHUA NAM HAI
发明人： ZUO JIANRU , CHUA NAM-HAI
IPC分类号： A01H1/00 , C12N5/10 , C12N15/09 , C12N15/82 , A01H5/00 , C12Q1/02 , C12Q1/68
CPC分类号： C12N15/8274 , C12N15/8209 , C12N15/8237 , C12N15/8238 , C12N15/8242 , C12N15/8295
摘要： Disclosed is a chemically inducible promoter for transforming plants or plant cells with genes which are regulatable by adding the plants or cells to a medium containing an inducer or by removing them from such medium. The promoter is inducible by a glucocorticoid, estrogen or inducer not endogenous to plants. Such promoters may be used with any plant genes that can promote shoot regeneration and development to induce shoot formation in the presence of a glucocorticoid, estrogen or inducer. The promoter may be used with antibiotic or herbicide resistance genes or other genes which are regulatable by the presence or absence of a given inducer. Also presented are organisms or cells comprising a gene wherein the natural promoter of the gene is disrupted and the gene is placed under the control of a transgenic inducible promoter. These organisms and cells and their progeny are useful for screening for conditional gain of function and loss of function mutations.
摘要翻译：公开了一种化学诱导型启动子，用于通过将植物或细胞添加到含有诱导物的培养基中或通过从这种培养基中除去可调节基因来转化植物或植物细胞。启动子可通过糖皮质激素，雌激素或对植物无内源性的诱导物诱导。这样的启动子可以与任何植物基因一起使用，所述植物基因可以在糖皮质激素，雌激素或诱导物的存在下促进芽再生和发育以诱导芽形成。启动子可以与抗生素或除草剂抗性基因或其它可通过给定诱导物的存在或不存在而调节的基因一起使用。还提供了包含基因的生物体或细胞，其中基因的天然启动子被破坏并且将基因置于转基因诱导型启动子的控制下。这些生物和细胞及其后代可用于筛选功能条件增加和功能突变丧失。

4. 发明申请

WO1994012015A1 EXPRESSION MOTIFS THAT CONFER TISSUE- AND DEVELOPMENTAL-SPECIFIC EXPRESSION IN PLANTS 审中-公开
标题翻译：在工厂中组织组织和开发特定表达的表达运动
公开(公告)号：WO1994012015A1
公开(公告)日：1994-06-09
申请号：PCT/US1993011516
申请日：1993-11-29
申请人： CHUA, Nam-Hai
IPC分类号： A01H05/00
CPC分类号： C12N15/8227 , C12N15/8222 , C12N15/8234
摘要： The present invention relates to the use of DNA sequence motifs to regulate gene expression in a tissue- or developmental-specific manner in transgenic plants. The invention generally relates to the engineering and use of G-box related sequence motifs, specifically Iwt and PA motifs, which function as cis-elements of promoters, to regulate the expression of heterologous genes in transgenic plants. PA enhances high level expression in roots, low level expression in leaves and little or no expression in seeds. By contrast, Iwt confers preferential expression in seeds, but in a developmentally-regulated manner.
摘要翻译：本发明涉及DNA序列基序在转基因植物中以组织或发育特异性方式调节基因表达的用途。本发明一般涉及用作调节转基因植物中异源基因表达的G盒相关序列基序，特别是Iwt和PA基序的工程和应用，其作为启动子的顺式元件。 PA增强根中的高水平表达，叶中低水平表达，并且在种子中几乎没有表达。相比之下，Iwt赋予种子优先表达，但以发育调节的方式。

5. 发明申请

WO2011040880A1 CONTROL OF PESTS IN PLANTS 审中-公开
标题翻译：植物中的植物控制
公开(公告)号：WO2011040880A1
公开(公告)日：2011-04-07
申请号：PCT/SG2010/000339
申请日：2010-09-15
申请人： TEMASEK LIFE SCIENCES LABORATORY LIMITED , YE, Jian , CHUA, Nam-Hai , QU, Jing , GAO, Shi-Qiang
发明人： YE, Jian , CHUA, Nam-Hai , QU, Jing , GAO, Shi-Qiang
IPC分类号： C12N15/83
CPC分类号： C12N15/8218 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： The present invention relates to the field of controlling pests, such as insects, using a virus to express pest genes in hosts. More specifically, the present invention relates to a method for rapidly screening for pest genes which can lead to mortality of the pest when the pest has ingested host tissues expressing virus-linked pest gene sequences. The present invention also relates to a method for controlling pests by viral expression of target pest sequences to modify endogenous expression of pest genes in cells or tissues of the pest.
摘要翻译：本发明涉及使用病毒来在宿主中表达有害生物基因的昆虫等害虫防治领域。更具体地说，本发明涉及一种用于快速筛选有害生物基因的方法，当害虫摄入表达病毒连接的害虫基因序列的宿主组织时，可导致害虫死亡。本发明还涉及通过靶病毒序列的病毒表达来控制害虫的方法，以修饰害虫的细胞或组织中害虫基因的内源性表达。

6. 发明申请

WO2010080071A1 FUNCTIONAL ANALYSIS OF JATROPHA CURCAS GENES 审中-公开
标题翻译： JATROPHA CURCAS基因的功能分析
公开(公告)号：WO2010080071A1
公开(公告)日：2010-07-15
申请号：PCT/SG2009/000481
申请日：2009-12-16
申请人： TEMASEK LIFE SCIENCES LABORATORY LIMITED , YE, Jian , CHUA, Nam Hai , QU, Jing
发明人： YE, Jian , CHUA, Nam Hai , QU, Jing
IPC分类号： C12N15/83 , A01H1/06
CPC分类号： C12N15/8218
摘要： The present invention relates to the field of functional analysis of Jatropha genes on a genomic scale. More specifically, the present invention relates to a method for high-throughtput functional analysis of Jatropha curcas genes on a genomic scale using virus-induced gene silencing. The method involves use of the tobacco rattle virus (TRV).
摘要翻译：本发明涉及基因组尺度上的麻疯树基因的功能分析领域。更具体地，本发明涉及使用病毒诱导的基因沉默在基因组规模上高麻醉麻疯树基因的高通量功能分析的方法。该方法涉及使用烟草拨浪鼓病毒（TRV）。

7. 发明申请

WO2009008837A1 LEXA-BASED CHEMICAL-INDUCIBLE GENE EXPRESSION SYSTEM FOR USE IN TRANSGENIC ANIMALS 审中-公开
标题翻译：用于转基因动物的基于LEXA的化学诱导基因表达系统
公开(公告)号：WO2009008837A1
公开(公告)日：2009-01-15
申请号：PCT/SG2008/000237
申请日：2008-07-02
申请人： TEMASEK LIFE SCIENCES LABORATORY LIMITED , EMELYANOV, Alexander , PARINOV, Sergey , CHUA, Nam-Hai
发明人： EMELYANOV, Alexander , PARINOV, Sergey , CHUA, Nam-Hai
IPC分类号： C12N15/85 , A01K67/027 , C12N15/63
CPC分类号： C12N15/85 , A01K67/0275 , A01K2217/052 , A01K2217/15 , A01K2217/203 , A01K2227/40 , A01K2267/03 , A01K2267/0393 , C07K2319/71 , C07K2319/715 , C07K2319/80 , C12N15/8509 , C12N2800/40 , C12N2830/002
摘要： The present invention relates generally to chemical -inducible system and to methods of use in transgenic animals. More specifically, the present invention relates to a chimeric transcription factor that binds to a ligand and functions in ligand-dependent manner to induce expression of genes of interest under the control of a synthetic operator-promoter sequence. The expression of genes of interest can be tightly controlled by adding or removing the ligand.
摘要翻译：本发明一般涉及化学诱导系统和转基因动物中使用的方法。更具体地，本发明涉及结合配体并以配体依赖性方式起作用以在合成的操作者 - 启动子序列的控制下诱导感兴趣的基因表达的嵌合转录因子。感兴趣的基因的表达可以通过添加或除去配体来严格控制。

8. 发明申请

WO2015158919A1 MAIZE PLASTID TRANSFORMATION METHOD 审中-公开
标题翻译： MAIZE PLASTID转换方法
公开(公告)号：WO2015158919A1
公开(公告)日：2015-10-22
申请号：PCT/EP2015/058449
申请日：2015-04-17
申请人： MØLLER, Simon Geir , DUNNE, Aisling , CHUA, Nam-Hai
发明人： MØLLER, Simon Geir , DUNNE, Aisling , CHUA, Nam-Hai
IPC分类号： C12N15/82
CPC分类号： C12N15/821 , A01H4/008 , C12N15/8201 , C12N15/8207 , C12N15/8213 , C12N15/8214 , C12N15/8294
摘要： The present invention relates to processes for the transformation of plant tissues with a genetic construct which comprises a transgene and a selection gene. The selection gene preferably encodes an auxin biosynthetic polypeptide, thus allowing for selection of transformed plants on media lacking plant auxins. The invention particularly relates to processes wherein the selection step is carried out under a light/dark cycle.
摘要翻译：本发明涉及用包含转基因和选择基因的遗传构建体转化植物组织的方法。选择基因优选编码生长素生物合成多肽，从而允许在缺乏植物生长素的培养基上选择转化的植物。本发明特别涉及其中选择步骤在光/暗循环下进行的方法。

9. 发明申请

WO2010144058A8 VIRUS INDUCED GENE SILENCING (VIGS) FOR FUNCTIONAL ANALYSIS OF GENES IN COTTON. 审中-公开
标题翻译： VIRS诱导基因沉默（VIGS）用于棉花中基因的功能分析。
公开(公告)号：WO2010144058A8
公开(公告)日：2011-03-31
申请号：PCT/SG2010000220
申请日：2010-06-10
申请人： TEMASEK LIFE SCIENCES LAB LTD , YE JIAN , CHUA NAM HAI , QU JING , GENG YUN FENG , BU YUN PING
发明人： YE JIAN , CHUA NAM HAI , QU JING , GENG YUN FENG , BU YUN PING
IPC分类号： C12N15/83 , C12N15/82
CPC分类号： C12N15/8218 , C12N15/8261 , Y02A40/146
摘要： The invention relates to the functional analysis of genes in cotton by employing the Virus Induced Gene Silencing (VIGS) method. More specifically this method induces gene silencing in cotton with the help of the Tobacco Rattle Virus (TRV) vectors RNAl and RNA2 and phenotypic effects on the cotton plant can be analysed Moreover this invention also provides transient expression vector TRV RNA2 in order to transiently express genes in cotton plants and plant tissue under the influence of a strong subgenomic promoter.
摘要翻译：本发明涉及使用病毒诱导基因沉默（VIGS）方法对棉花中基因的功能分析。更具体地说，这种方法在烟草叮咬病毒（TRV）载体RNA1和RNA2的帮助下诱导棉花中的基因沉默，并且可以分析对棉花植物的表型效应。此外，本发明还提供瞬时表达载体TRV RNA2以瞬时表达基因在棉花植物和植物组织中受到强亚基因子启动子的影响。

10. 发明申请

WO2008143786A1 PRODUCTION OF ARTIFICIAL MICRORNAS USING SYNTHETIC MICRORNA PRECURSORS 审中-公开
标题翻译：使用合成微生物前体制备人造微生物
公开(公告)号：WO2008143786A1
公开(公告)日：2008-11-27
申请号：PCT/US2008/005862
申请日：2008-05-08
申请人： THE ROCKEFELLER UNIVERSITY , CHUA, Nam-hai , NIU, Qi-wen
发明人： CHUA, Nam-hai , NIU, Qi-wen
IPC分类号： A01H9/00
CPC分类号： C12N15/8218 , C12N15/113 , C12N2310/141
摘要： The invention provides methods and compositions useful in target sequence suppression, target sequence validation and target sequence down regulation. The invention provides polynucleotide constructs useful for producing artificial microRNA (amiRNA) using synthetic amiRNA precursors.
摘要翻译：本发明提供了可用于靶序列抑制，靶序列验证和靶序列下调的方法和组合。本发明提供了可用于使用合成的amiRNA前体产生人工微小RNA（amiRNA）的多核苷酸构建体。

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