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1. 发明申请

WO2010052337A3 BIOANALYTICAL PROCEDURE TO DETECT MEMBRANE RECEPTOR ACTIVATION 审中-公开
标题翻译：检测膜受体活化的生物学方法
公开(公告)号：WO2010052337A3
公开(公告)日：2010-07-01
申请号：PCT/EP2009064907
申请日：2009-11-10
申请人： ECOLE POLYTECH , PICK HORST MATTHIAS , VOGEL HORST
发明人： PICK HORST MATTHIAS , VOGEL HORST
IPC分类号： G01N33/543 , G01N33/68
CPC分类号： G01N33/567 , B82Y30/00 , G01N33/554 , G01N33/6872 , G01N2500/04
摘要： The invention concerns a method for screening compound interaction with transmembrane receptors involved in cellular signal transduction, preferably with G protein coupled receptors, by adding the compound to be analyzed into a medium comprising native inverted vesicles with at least one said transmembrane receptor whose native intracellular side is located on the outer surface of the inverted vesicle.
摘要翻译：本发明涉及通过将待分析的化合物加入包含天然倒卵泡的培养基中的至少一种所述跨膜受体（其天然细胞内侧面）来筛选与参与细胞信号转导的跨膜受体的化合物相互作用的方法，所述跨膜受体优选与G蛋白偶联受体位于倒置囊泡的外表面。

2. 发明申请

WO2006105985A1 REVERSIBLE SEQUENTIAL BINDING ASSAYS 审中-公开
标题翻译：可逆序列绑定测试
公开(公告)号：WO2006105985A1
公开(公告)日：2006-10-12
申请号：PCT/EP2006/003201
申请日：2006-04-07
申请人： EPFL-SWISS FEDERAL INSTITUTE OF TECHNOLOGY-LAUSANNE , SCHREITER, Christoph , SEGURA, Jean-Manuel , VOGEL, Horst , HOVIUS, Rudolf , MARTINEZ, Karen , GJONI, Marinela
发明人： SCHREITER, Christoph , SEGURA, Jean-Manuel , VOGEL, Horst , HOVIUS, Rudolf , MARTINEZ, Karen , GJONI, Marinela
IPC分类号： G01N33/58 , G01N33/566 , G01N33/68 , C07K1/13
CPC分类号： G01N33/582 , G01N33/566 , G01N33/6872 , G01N2333/70571
摘要： The present invention relates to a novel method for determining a receptor- ligand interaction which is suitable for ultra sensitive pharmacological receptor profiling on cells, e.g. single living cells based on the repetitive application of reversibly binding fluorescent ligands with fast association- dissociation kinetics. The method allows the determination of single receptor molecules and ligand binding cycles on single cells in microfluidic structures. Thus, the present method is important for high throughput screening of compounds, membrane receptors and tissues. Further, novel fluorescent derivatives of α-conotoxins, particularly of α-conotoxin Gl as described.
摘要翻译：本发明涉及一种用于测定受体 - 配体相互作用的新方法，该方法适用于细胞上的超敏感药理学受体分析。基于可逆结合荧光配体的快速缔合解离动力学的单一活细胞。该方法允许在微流体结构中的单细胞上测定单受体分子和配体结合循环。因此，本方法对于化合物，膜受体和组织的高通量筛选是重要的。此外，α-芋螺毒素，特别是α-芋螺毒素Gl的新型荧光衍生物如上所述。

3. 发明申请

WO1993021528A1 LIPID MEMBRANE SENSORS 审中-公开
标题翻译： LIPID膜传感器
公开(公告)号：WO1993021528A1
公开(公告)日：1993-10-28
申请号：PCT/EP1993000976
申请日：1993-04-21
申请人： EUROPEAN INSTITUTE OF TECHNOLOGY , LANG, Holger , KOENIG, Bernd , VOGEL, Horst
发明人： EUROPEAN INSTITUTE OF TECHNOLOGY
IPC分类号： G01N33/543
CPC分类号： C12Q1/001 , G01N33/5432 , Y10S436/806
摘要： A bilayer lipid membrane (BLM) sensor comprising (1) a gold recording surface, (2) a first lipid layer which is an imperfect layer of a thiolipid which comprises the residue of two phospholipid molecules linked to each end of a disulphide (-S-S-) group, each through an oxyethylene (-O-CH2-CH2) chain which is short enough to allow the thiolipid to become anchored to the gold surface by self-assembly, but long enough to trap an aqueous layer between the gold surface and the bottom of the thiolipid layer, said thiolipid being attached to the gold surface and said imperfect layer being completed by a phospholipid which provides an unattached fluid phase at room temperature, and (3) a second lipid layer of phospholipid. The sensor is especially useful for measuring changes in electrical resistance and/or capacity when it is prepared to contain a receptor protein and an analyte containing an agonist to the receptor is contacted with the bilayer.
摘要翻译：包含（1）金记录表面的双层脂质膜（BLM）传感器，（2）作为硫醇脂不完美层的第一脂质层，其包含与二硫键的每个末端连接的两个磷脂分子的残基（-SS - ）基团，各自通过氧化乙烯（-O-CH 2 -CH 2）链，其足够短以允许硫醇脂通过自组装而锚定到金表面，但足够长以在水表面和金表面之间捕获水层所述硫醇脂层的底部，所述硫醇脂连接到金表面，并且所述不完美层由在室温下提供未连接的流体相的磷脂完成，和（3）磷脂的第二脂质层。当测定受体蛋白质的含量时，传感器特别适用于测量电阻和/或容量的变化，并且含有受体的激动剂的分析物与双层接触。

4. 发明申请

WO2006013109A1 METHOD FOR TESTING A SUBSTANCE INTERACTING WITH A TARGET MOLECULE 审中-公开
标题翻译：用于测试与目标分子相互作用的物质的方法
公开(公告)号：WO2006013109A1
公开(公告)日：2006-02-09
申请号：PCT/EP2005/008510
申请日：2005-08-05
申请人： EPFL SWISS FEDERAL INSTITUTE OF TECHNOLOGY-LAUSANNE , JANKEVICS, Hanna , PRUMMER, Michael , VOGEL, Horst
发明人： JANKEVICS, Hanna , PRUMMER, Michael , VOGEL, Horst
IPC分类号： G01N21/64 , G01N33/543 , G01N33/554
CPC分类号： G01N33/5091 , G01N21/6428 , G01N33/5008 , G01N33/502 , G01N2333/72
摘要： The present invention relates to a method for testing a substance interacting with a target molecule in a cell by fluorescence correlation spectroscopy (FCS) by adding a test substance to the cell and determining a distribution of diffusion coefficients for the fluorescently labelled target molecule. Preferably, the target molecule is a nuclear hormone receptor, e.g. the estrogen receptor.
摘要翻译：本发明涉及一种通过荧光相关光谱法（FCS），通过向细胞中加入测试物质并确定荧光标记的靶分子的扩散系数的分布来测试与细胞中的靶分子相互作用的物质的方法。优选地，靶分子是核激素受体，例如。雌激素受体。

5. 发明申请

WO2005121226A1 CELLULOSE MODEL SURFACE 审中-公开
标题翻译：纤维素模型表面
公开(公告)号：WO2005121226A1
公开(公告)日：2005-12-22
申请号：PCT/IB2005/001479
申请日：2005-05-27
申请人： FIRMENICH SA , VARNOUS, Mahtab , VOGEL, Horst , ULRICH, Wolf-Peter , STORA, Thierry , LEUFGEN, Kirsten , VELAZCO, Maria Inés
发明人： VARNOUS, Mahtab , VOGEL, Horst , ULRICH, Wolf-Peter , STORA, Thierry , LEUFGEN, Kirsten , VELAZCO, Maria Inés
IPC分类号： C08J5/18
CPC分类号： C08B16/00 , C08J5/18 , C08J2301/08 , C08L1/08 , C09D101/08 , G01N21/05
摘要： The present invention relates to an isotropic cellulose film deposited on an inorganic layer. The present invention concerns also a process to manufacture such cellulose films as well as an analytical cell comprising such deposited films. Said cellulose film is particularly useful to study the interactions or performances of various chemicals with a cellulose surface.
摘要翻译：本发明涉及沉积在无机层上的各向同性纤维素膜。本发明还涉及制造这种纤维素膜的方法以及包括这种沉积膜的分析单元。所述纤维素膜特别可用于研究具有纤维素表面的各种化学品的相互作用或性能。

6. 发明申请

WO2003052136A3 SEQUENZIERUNG ÜBER LOCHMEMBRANEN 审中-公开
公开(公告)号：WO2003052136A3
公开(公告)日：2003-06-26
申请号：PCT/EP2002/014489
申请日：2002-12-18
申请人： GNOTHIS HOLDING SA , RIGLER, Rudolf , VOGEL, Horst
发明人： RIGLER, Rudolf , VOGEL, Horst
IPC分类号： C12Q1/68
摘要： Die Erfindung betrifft ein Verfahren und eine Vorrichtung zur Sequenzierung von Nukleinsäuremolekülen über eine Lochmembran. Insbesondere kann die Erfindung zur Multiplex-Sequenzierung eingesetzt werden.

7. 发明申请

WO2002046766A3 BIOANALYTISCHES REAGENS VERFAHREN ZU DESSEN HERSTELLUNG UND SENSORPLATTFORMEN SOWIE NACHWEISVERFAHREN BERUHEND AUF DER VERWENDUNG DES BIOANALYTISCHEN REAGENS 审中-公开
公开(公告)号：WO2002046766A3
公开(公告)日：2002-06-13
申请号：PCT/EP2001/014134
申请日：2001-12-04
申请人： ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE , ZEPTOSENS AG , VOGEL, Horst , PICK, Horst, M. , PREUSS, Axel, K. , TAIRI, Ana-Paula , SCHMID-OSBORNE, Evelyne , PAWLAK, Michael
发明人： VOGEL, Horst , PICK, Horst, M. , PREUSS, Axel, K. , TAIRI, Ana-Paula , SCHMID-OSBORNE, Evelyne , PAWLAK, Michael
IPC分类号： G01N33/554
摘要： Die Erfindung betrifft verschiedene Ausführungsformen eines bioanalytisches Reagens mit mindestens einem Vesikel, das aus einer lebenden Zelle erzeugt wird, umfassend mindestens einen Rezeptor, dadurch gekennzeichnet, dass ein von diesem Rezeptor in der verwendeten lebenden Zelle ausgelöster Mechanismus einer Signaltransduktion in besagtem Vesikel als Bestandteil des bioanalytischen Reagens erhalten bleibt. Die Erfindung betrifft ausserdem Verfahren zur Herstellung eines erfindungsgemässen bioanalytischen Reagens sowie auf dem Einsatz dieses Reagens basierende bioanalytische Nachweisverfahren sowie die verwendung dieses Nachweisverfahren und des bioanalytischen Reagens.

8. 发明申请

WO1996038726A1 COVALENTLY IMMOBILIZED PHOSPHOLIPID BILAYERS ON SOLID SURFACES 审中-公开
标题翻译：在固体表面上共同固定磷脂双酚
公开(公告)号：WO1996038726A1
公开(公告)日：1996-12-05
申请号：PCT/IB1996000496
申请日：1996-05-24
申请人： ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL) , HEYSE, Stephan , SÄNGER, Michael , SIGRIST, Hans , VOGEL, Horst
发明人： ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)
IPC分类号： G01N33/543
CPC分类号： C12Q1/002 , A61L27/34 , A61L29/085 , B01J2219/00734 , G01N33/5438
摘要： A solid coated device having covalently attached a coating which comprises a first layer containing first functional groups, to which first layer functional groups is covalently attached a second, linking layer carrying second functional groups, to which second, linking layer is covalently attached a third, proximal phospholipid layer, which third, proximal phospholipid layer, to which third, proximal phospholipid layer is not-covalently attached a fourth, distal lipid layer, so that the proximal and the distal lipid layers together form a bilayer, which lipid bilayer, into which lipid bilayer are optionally inserted receptor molecules, method of the preparation thereof, and uses therof as biosensors or implantation devices.
摘要翻译：一种具有共价连接涂层的固体涂覆装置，该涂层包括含有第一官能团的第一层，第一层官能团共价连接到载有第二官能团的第二连接层，第二层连接层共同连接第三层，近端磷脂层，其中第三近端磷脂层，其中第三近端磷脂层不共价连接第四远端脂质层，使得近端和远端脂质层一起形成双层，其中脂质双层，其中脂质双层是任选插入的受体分子，其制备方法，并使用该生物传感器或植入装置。

9. 发明申请

WO2010052337A2 BIOANALYTICAL PROCEDURE TO DETECT MEMBRANE RECEPTOR ACTIVATION 审中-公开
标题翻译：生物分布检测膜受体激活的方法
公开(公告)号：WO2010052337A2
公开(公告)日：2010-05-14
申请号：PCT/EP2009/064907
申请日：2009-11-10
申请人： ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL) , PICK, Horst Matthias , VOGEL, Horst
发明人： PICK, Horst Matthias , VOGEL, Horst
IPC分类号： G01N33/543 , G01N33/68
CPC分类号： G01N33/567 , B82Y30/00 , G01N33/554 , G01N33/6872 , G01N2500/04
摘要： The invention concerns a method for screening compound interaction with transmembrane receptors involved in cellular signal transduction, preferably with G protein coupled receptors, by adding the compound to be analyzed into a medium comprising native inverted vesicles with at least one said transmembrane receptor whose native intracellular side is located on the outer surface of the inverted vesicle.
摘要翻译：本发明涉及用于筛选与参与细胞信号转导的跨膜受体（优选G蛋白偶联受体）的化合物相互作用的方法，其通过将待分析的化合物添加到包含天然倒置小泡的培养基中一个说是跨膜受体，其天然细胞内侧位于倒置囊泡的外表面上。

10. 发明申请

WO1999005509A1 DETECTION AND INVESTIGATION OF BIOLOGICAL MOLECULES BY FOURIER TRANSFORM INFRA-RED SPECTROSCOPY 审中-公开
标题翻译：通过FOURIER TRANSFORM红外光谱检测和调查生物分子
公开(公告)号：WO1999005509A1
公开(公告)日：1999-02-04
申请号：PCT/IB1997000920
申请日：1997-07-24
申请人： ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE , VOGEL, Horst , KELLER, Thomas , LILEY, Martha
发明人： ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE
IPC分类号： G01N21/55
CPC分类号： B82Y30/00 , B82Y15/00 , G01N21/552
摘要： Method and apparatus to study biological molecules or biological components at or in self-assembled monolayers (SAMs) on metal surfaces (2) such as gold in aqueous environments (6) using attenuated total internal reflection Fourier transform infra-red (ATR-FTIR) spectroscopy are described. Said method may be used for e.g. the screening for effective substances suitable as agonists or antagonists or - if performed time resolved - for studying kinetics of interactions of e.g. biological molecules adsorption and desorption processes or for the development of materials and surfaces for biological or medical applications.
摘要翻译：在金属表面（2）上的自组装单层（SAM）中或在含水环境中的金（6）中使用衰减的全内反射傅立叶变换红外（ATR-FTIR）研究生物分子或生物成分的方法和装置描述光谱。所述方法可以用于例如。筛选适合作为激动剂或拮抗剂的有效物质，或者 - 如果执行时间解决 - 用于研究例如抗体的相互作用的动力学。生物分子吸附和解吸过程或用于生物或医学应用的材料和表面的开发。

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