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1. 发明申请

WO2010118300A1 CONFORMATIONAL EPITOPE INITIATED SIGNAL AMPLIFICATION 审中-公开
标题翻译：合成EPITOPE启动信号放大
公开(公告)号：WO2010118300A1
公开(公告)日：2010-10-14
申请号：PCT/US2010/030505
申请日：2010-04-09
申请人： UNGER, John T. , HILFIKER, Rolf
发明人： UNGER, John T. , HILFIKER, Rolf
IPC分类号： G01N33/53
CPC分类号： G01N33/557 , C12Q1/6804 , C12Q2565/101 , C12Q2525/205
摘要： This invention relates to a method to generate a signal used to detect the presence or quantity of a biomarker in a sample. The signal generating reaction is initiated when the biomarker under assay interacts with a specific binding partner. The interaction produces a structural change in the binding partner that is recognized by additional binding partners capable of generating a signal. The reaction produces a localized cluster of signaling molecules that can be detected above background. The signaling cluster is detectable within minutes when interrogated in a chamber of specific dimensions. The presence of the signaling clusters is a qualitative indication of the presence of the analyte, while the number of signaling clusters detected is a direct quantification of the number of biomarker molecules in the sample. The reaction can be formatted to detect proteins, nucleic acids, cells or other informative biomarkers.
摘要翻译：本发明涉及生成用于检测样品中生物标志物的存在或数量的信号的方法。当测定中的生物标志物与特异性结合配偶体相互作用时，起始信号产生反应。相互作用产生由能够产生信号的另外的结合配偶体识别的结合配偶体的结构变化。该反应产生可以在背景以上检测到的信号分子的局部簇。当在特定尺寸的室中询问时，信号簇在几分钟内可被检测。信号簇的存在是分析物存在的定性指示，而检测到的信号簇的数量是对样品中生物标记分子数量的直接定量。反应可以被格式化以检测蛋白质，核酸，细胞或其他信息生物标志物。

2. 发明申请

WO1995014790A1 USE OF ANTISENSE OLIGOMERS IN A PROCESS FOR CONTROLLING CONTAMINATION IN NUCLEIC ACID AMPLIFICATION REACTIONS 审中-公开
标题翻译：在控制核酸放大反应中的污染过程中使用抗生素寡聚体
公开(公告)号：WO1995014790A1
公开(公告)日：1995-06-01
申请号：PCT/IB1994000366
申请日：1994-11-21
申请人： CIBA CORNING DIAGNOSTICS CORP. , LUDTKE, Douglas, N. , MONAHAN, John, E. , UNGER, John, T.
发明人： CIBA CORNING DIAGNOSTICS CORP.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6848 , C12Q1/682 , C12Q1/6867 , C12Q2549/126
摘要： A novel process for the use of antisense oligonucleotides and analogs thereof has been developed. Namely, this technique is useful for the elimination of contamination in the nucleic acid amplification area. Elimination of unwanted contamination has made gene probe analyses much more reproducible.
摘要翻译：已经开发了一种使用反义寡核苷酸及其类似物的新方法。也就是说，该技术对于消除核酸扩增区域中的污染是有用的。消除不想要的污染使得基因探针分析更加可重现。

3. 发明申请

WO1997001763A1 NON-SEPARATION SPECIFIC BINDING CHEMILUMINESCENT ASSAY 审中-公开
标题翻译：非分离特异性结合光亮度测定
公开(公告)号：WO1997001763A1
公开(公告)日：1997-01-16
申请号：PCT/IB1996000617
申请日：1996-06-27
申请人： CHIRON DIAGNOSTICS CORPORATION , BARLOW, Eve, H. , CARROLL, Eddie, III , CONNOLLY, Joseph, E. , LEE, Michael, J. , MARTINELLI, Richard, A. , UNGER, John, T.
发明人： CHIRON DIAGNOSTICS CORPORATION
IPC分类号： G01N33/58
CPC分类号： G01N33/542 , G01N33/582
摘要： The assay described herein is predicated on an observation that when acridinium ester labeled tracers are bound to their corresponding binding conjugate immobilized on a metal oxide solid phase, the measurable chemiluminescent light emission of the labeled tracer bound to the solid phase is quenched as compared to the free fraction tracer that is unattached to the solid phase. According to the invention, a non-separation specific binding assay to detect or quantify the presence of an analyte is provided where the entire reaction mixture is flashed (including unreacted tracer) and modulated signal (because of the quench effect) is associated with a reference, thus determining the amount or presence of said analyte in said sample. Disadvantages inherent in heterogeneous assays employing multiple separations may be avoided using this non-separation method.
摘要翻译：本文所述的测定是基于以下观察结果：当吖啶酯标记的示踪剂与其固定在金属氧化物固相上的相应结合缀合物结合时，与固相结合的标记示踪剂的可测量化学发光光发射与与固相无关的游离级分示踪剂。根据本发明，提供了检测或定量分析物存在的非分离特异性结合测定法，其中整个反应混合物闪蒸（包括未反应的示踪剂）和调制信号（由于骤冷作用）与参考文献相关联，从而确定所述样品中所述分析物的量或存在。使用这种非分离方法可以避免使用多次分离的异质测定中固有的缺点。

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