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1. 发明申请

WO2010118300A1 CONFORMATIONAL EPITOPE INITIATED SIGNAL AMPLIFICATION 审中-公开
标题翻译：合成EPITOPE启动信号放大
公开(公告)号：WO2010118300A1
公开(公告)日：2010-10-14
申请号：PCT/US2010/030505
申请日：2010-04-09
申请人： UNGER, John T. , HILFIKER, Rolf
发明人： UNGER, John T. , HILFIKER, Rolf
IPC分类号： G01N33/53
CPC分类号： G01N33/557 , C12Q1/6804 , C12Q2565/101 , C12Q2525/205
摘要： This invention relates to a method to generate a signal used to detect the presence or quantity of a biomarker in a sample. The signal generating reaction is initiated when the biomarker under assay interacts with a specific binding partner. The interaction produces a structural change in the binding partner that is recognized by additional binding partners capable of generating a signal. The reaction produces a localized cluster of signaling molecules that can be detected above background. The signaling cluster is detectable within minutes when interrogated in a chamber of specific dimensions. The presence of the signaling clusters is a qualitative indication of the presence of the analyte, while the number of signaling clusters detected is a direct quantification of the number of biomarker molecules in the sample. The reaction can be formatted to detect proteins, nucleic acids, cells or other informative biomarkers.
摘要翻译：本发明涉及生成用于检测样品中生物标志物的存在或数量的信号的方法。当测定中的生物标志物与特异性结合配偶体相互作用时，起始信号产生反应。相互作用产生由能够产生信号的另外的结合配偶体识别的结合配偶体的结构变化。该反应产生可以在背景以上检测到的信号分子的局部簇。当在特定尺寸的室中询问时，信号簇在几分钟内可被检测。信号簇的存在是分析物存在的定性指示，而检测到的信号簇的数量是对样品中生物标记分子数量的直接定量。反应可以被格式化以检测蛋白质，核酸，细胞或其他信息生物标志物。

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