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1. 发明申请

WO1994005771A2 NEW BACILLUS THURINGIENSIS STRAINS AND THEIR INSECTICIDAL PROTEINS 审中-公开
标题翻译：新的BACILLUS THURINGIENSIS菌株及其杀虫蛋白
公开(公告)号：WO1994005771A2
公开(公告)日：1994-03-17
申请号：PCT/EP1993001820
申请日：1993-07-12
申请人： PLANT GENETIC SYSTEMS N.V. , LAMBERT, Bart , JANSENS, Stefan , VAN AUDENHOVE, Katrien , PEFEROEN, Marnix
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N01/20
CPC分类号： C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinia nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.
摘要翻译：四种新型苏云金芽孢杆菌菌株，其保藏在BCCM-LMG，保藏号为。 LMG P-12592，LMG P-12593，LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质，更特别地针对夜蛾科（Spodoptera spp）等等。和Agrotis ipsilon，针对鞭毛虫如鸵鸟（Ostrinia nubilalis），以及针对pon科（Yponomeutidae）如小菜蛾（Plutella xylostella），并且由新基因编码。晶体蛋白含有原毒素，其可以产生作为胰蛋白酶消化产物的毒素。一种植物，其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化，对鳞翅目具有抗性。每种菌株本身或其晶体，晶体蛋白质，原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。

2. 发明申请

WO1993021335A2 TRANSFORMATION OF MONOCOT CELLS 审中-公开
标题翻译：单核细胞的转化
公开(公告)号：WO1993021335A2
公开(公告)日：1993-10-28
申请号：PCT/EP1993000905
申请日：1993-04-14
申请人： PLANT GENETIC SYSTEMS, N.V. , GOBEL, Elke , NAKAKIDO, Fumio
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/87
CPC分类号： C12N5/04 , C12N15/8206
摘要： A process for genetically transforming the nuclear genome of a cell of a monocotyledonous plant, particularly a gramineous plant, comprising the step of: electroporating, with one or more DNA fragments, either a culture of suspended cells of the plant or cells of the plant capable of forming the culture, each cell possessing at least part of its plant cell wall.
摘要翻译：一种用于遗传转化单子叶植物，特别是禾本科植物的细胞的核基因组的方法，其包括以下步骤：用一个或多个DNA片段电穿孔植物的悬浮细胞或能够植物的细胞的培养物形成培养物，每个细胞具有其植物细胞壁的至少一部分。

3. 发明申请

WO1991016432A1 MODIFIED BACILLUS THURINGIENSIS INSECTICIDAL-CRYSTAL PROTEIN GENES AND THEIR EXPRESSION IN PLANT CELLS 审中-公开
标题翻译：改良的BACILLUS THURINGIENSIS INSECTICIDAL-CRYSTAL蛋白基因及其在植物细胞中的表达
公开(公告)号：WO1991016432A1
公开(公告)日：1991-10-31
申请号：PCT/EP1991000733
申请日：1991-04-17
申请人： PLANT GENETIC SYSTEMS N.V. , CORNELISSEN, Marc , SOETAERT, Piet , STAM, Maike , DOCKX, Jan
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N15/31
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： A DNA fragment, encoding all or an insecticidally-effective part of a Bt crystal protein, is modified by changing A and T sequences to corresponding G and C sequences encoding the same amino acids.
摘要翻译：通过将A和T序列改变成编码相同氨基酸的相应G和C序列来修饰编码Bt结晶蛋白的全部或杀虫有效部分的DNA片段。

4. 发明申请

WO1990004032A1 A PROCESS FOR THE PRODUCTION OF TRANSGENIC PLANTS WITH INCREASED NUTRITIONAL VALUE VIA THE EXPRESSION OF MODIFIED 2S STORAGE ALBUMINS 审中-公开
标题翻译：通过修改的2S储存铝的表达生产具有增加的营养价值的转基因植物的方法
公开(公告)号：WO1990004032A1
公开(公告)日：1990-04-19
申请号：PCT/EP1989001229
申请日：1989-10-13
申请人： PLANT GENETIC SYSTEMS N.V. , EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA ... , DE CLERCQ, Ann , KREBBERS, Enno , VANDERKERCKHOVE, Joël , BARRETO DE CASTRO, Luiz , GANDER, Eugen , VAN MONTAGU, Marc
发明人： PLANT GENETIC SYSTEMS N.V. , EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA ...
IPC分类号： C12N15/82
CPC分类号： C07K14/00 , C07K14/415 , C07K2319/02 , C12N15/8251 , C12N15/8253 , C12N15/8254
摘要： The invention pertains to a process for producing transgenic plants with increased nutritional value. It comprises: cultivating plants obtained from regenerated plant cells or from seeds of plants obtained from said regenerated plant cells over one or several generations, whose genetic patrimony, replicable with said plants, comprises a precursor-coding nucleic acid sequence encoding the precursor of a 2S albumin storage protein and placed under the control of a promoter capable of directing gene expression in plants, said precursor-coding nucleic acid being modified in a nonessential region of its relevant sequence which encodes the mature 2S albumin or a subunit thereof with a nucleic acid insert in appropriate reading frame relationship with the surrounding part of said relevant sequence, said insert including a determined segment encoding an heterologous determined polypeptide containing appropriate aminoacid such as lysine and/or methionine and/or threonine and/or phenylalanine and/or trytophane and/or leucine and/or valine and/or isoleucine.
摘要翻译：本发明涉及生产营养价值增加的转基因植物的方法。它包括：从再生植物细胞获得的植物或从所述再生植物细胞获得的植物种子培育一代或几代，其遗传遗传可与所述植物复制，其包含编码2S前体的前体编码核酸序列白蛋白存储蛋白并置于能够引导植物中基因表达的启动子的控制下，所述前体编码核酸在其相关序列的非必需区域中被修饰，所述相关序列编码成熟的2S白蛋白或其亚基与核酸插入片段所述插入物包含编码含有适当氨基酸例如赖氨酸和/或甲硫氨酸和/或苏氨酸和/或苯丙氨酸和/或试ane酮的异源确定多肽的确定区段和/或亮氨酸和/或缬氨酸和/或异亮氨酸。

5. 发明申请

WO1989010396A1 PLANTS WITH MODIFIED STAMEN CELLS 审中-公开
标题翻译：具有修饰的STAMEN细胞的植物
公开(公告)号：WO1989010396A1
公开(公告)日：1989-11-02
申请号：PCT/EP1989000495
申请日：1989-04-27
申请人： PLANT GENETIC SYSTEMS N.V. , MARIANI, Celestina , LEEMANS, Jan , DE GREEF, Willy , DE BEUCKELEER, Marc
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N05/00
CPC分类号： C12Y302/01031 , C07K14/415 , C12N9/1085 , C12N9/16 , C12N9/22 , C12N9/2434 , C12N9/50 , C12N15/8222 , C12N15/8231 , C12N15/8289 , C12Y301/27003
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which selectively disrupts the metabolism, functioning and/or development of stamen cells of the plant. The foreign DNA sequence also optionally encodes a marker.

6. 发明申请

WO1997049814A1 GENE EXPRESSION IN PLANTS 审中-公开
标题翻译：植物中的基因表达
公开(公告)号：WO1997049814A1
公开(公告)日：1997-12-31
申请号：PCT/EP1997002832
申请日：1997-05-30
申请人： PLANT GENETIC SYSTEMS, N.V. , MEULEWAETER, Frank , CORNELISSEN, Marcus , VAN AARSSEN, Roel , SOETAERT, Piet , GOSSELE, Véronique
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/32
CPC分类号： C12N15/67 , C12N15/8216 , C12N15/8286 , Y02A40/162
摘要： The present invention provides chimeric genes that comprise a first promoter recognized by a DNA-dependent RNA polymerase different from a eukaryotic RNA polymerase II; a DNA region encoding a chimeric RNA which comprises a 5'UTR, an AU-rich heterologous coding sequence, a 3'UTR; and optionally a terminator sequence recognized by said RNA polymerase, wherein the first promoter and the DNA region encoding the chimeric RNA are operably linked such that upon transcription by the RNA polymerase an uncapped RNA species is produced which comprises a first translation enhancing sequence derived from the 5' region of genomic or subgenomic RNA of a positive stranded RNA plant virus; a heterologous RNA coding sequence encoding a polypeptide or protein of interest, preferably from an AT-rich gene; and a second translation enhancing sequence derived from the 3' region of genomic or subgenomic RNA of a positive-stranded RNA plant virus, wherein the uncapped RNA species is capable of being translated in the cytoplasm of a plant cell to produce the protein or polypeptide. Also provided in the invention are plant cells and plants comprising these chimeric genes, integrated in their nuclear DNA, whereby the plant cell produces the RNA polymerases corresponding to the used promoters and terminators. Further the invention provides a process for producing a plant expressing a protein or polypeptide encoded by a heterologous gene which comprises the steps of transforming the nuclear genome of a plant cell with the above-mentioned chimeric genes; and regenerating a transformed plant from the transformed cell.
摘要翻译：本发明提供包含由不同于真核RNA聚合酶II的DNA依赖性RNA聚合酶识别的第一启动子的嵌合基因; 编码嵌合RNA的DNA区域，其包含5'UTR，富含AU的异源编码序列，3'UTR; 并且任选地由所述RNA聚合酶识别的终止子序列，其中编码嵌合RNA的第一启动子和DNA区域可操作地连接，使得在通过RNA聚合酶转录时，产生未加帽的RNA物种，其包含衍生自所述RNA聚合酶的第一翻译增强序列 5'区域的正链RNA植物病毒的基因组或亚基因组RNA; 编码目标多肽或蛋白质的异源RNA编码序列，优选来自富含AT的基因; 和源自正链RNA植物病毒的基因组或亚基因组RNA的3'区域的第二翻译增强序列，其中未加帽的RNA物种能够在植物细胞的细胞质中翻译以产生蛋白质或多肽。本发明还提供了植物细胞和包含这些嵌合基因的植物，其整合在其核DNA中，由此植物细胞产生对应于所用促进剂和终止子的RNA聚合酶。此外，本发明提供了一种生产表达由异源基因编码的蛋白质或多肽的植物的方法，其包括用上述嵌合基因转化植物细胞的核基因组的步骤; 并从转化细胞再生转化的植物。

7. 发明申请

WO1992009696A1 PROCESS FOR TRANSFORMING MONOCOTYLEDONOUS PLANTS 审中-公开
标题翻译：改造单性植物的方法
公开(公告)号：WO1992009696A1
公开(公告)日：1992-06-11
申请号：PCT/EP1991002198
申请日：1991-11-21
申请人： PLANT GENETIC SYSTEMS, N.V. , D'HALLUIN, Kathleen , GÖBEL, Elke
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/82
CPC分类号： C12N15/8206 , C12N15/8289
摘要： Novel method of transforming monocotyledonous plants, particularly cereals, by wounding and/or degrading the cells of either intact tissue of the plant that is capable of forming compact embryogenic callus or the compact embryogenic callus itself.
摘要翻译：通过伤害和/或降解能够形成紧密的胚发生愈伤组织或致密的胚发生愈伤组织本身的植物的完整组织的细胞来转化单子叶植物，特别是谷类的新方法。

8. 发明申请

WO1991016433A1 NEW BACILLUS THURINGIENSIS STRAINS AND THEIR GENES ENCODING INSECTICIDAL TOXINS 审中-公开
标题翻译：新型香草芽孢杆菌菌株及其编码杀虫毒素的基因
公开(公告)号：WO1991016433A1
公开(公告)日：1991-10-31
申请号：PCT/EP1991000791
申请日：1991-04-24
申请人： PLANT GENETIC SYSTEMS N.V. , PEFEROEN, Marnix , LAMBERT, Bart , VAN ANDENHOVE, Katrien
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N15/32
CPC分类号： C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162 , Y10S435/832
摘要： Two new Bacillus thuringiensis strains, which are deposited at the DSM under accession nos 5870 and 5871, produce new crystal proteins during sporulation that are toxic to Coleoptera and that are encoded by new genes. The crystal proteins contain protoxins, which can yield toxins as trypsin-digestion products. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes an insecticidally effective portion of its respective protoxin or encodes its respective toxin, is resistant to Coleoptera. Each strain, itself, or its crystals, crystal proteins, protoxin, toxin and/or insecticidally effective protoxin portion can be used as the active ingredient in an insecticidal composition for combatting Coleoptera.
摘要翻译：在DSM下保藏的两种新的苏云金芽孢杆菌菌株在加入5870和5871下，在孢子形成过程中产生新的晶体蛋白，对鞘翅目有毒，并由新基因编码。晶体蛋白含有原毒素，其可产生作为胰蛋白酶消化产物的毒素。一种植物，其基因组用来自任一种菌株的DNA序列转化并编码其各自原毒素的杀虫有效部分或编码其各自的毒素，对鞘翅目具有抗性。每种菌株本身或其晶体，晶体蛋白质，原毒素，毒素和/或杀虫有效的前毒素部分都可以用作杀虫鞘组合物中的活性成分。

9. 发明申请

WO1991002068A1 PLANTS WITH MODIFIED FLOWERS, SEEDS OR EMBRYOS 审中-公开
标题翻译：具有改良花卉，种子或EMBRYOS的植物
公开(公告)号：WO1991002068A1
公开(公告)日：1991-02-21
申请号：PCT/EP1990001275
申请日：1990-08-01
申请人： PLANT GENETIC SYSTEMS, N.V. , DE GREEF, Willy , VAN EMMELO, John , DE OLIVEIRA, Dulce, Eleonora , DE SOUZA, Maria-Helena , VAN MONTAGU, Marc
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/82
CPC分类号： C12N9/22 , C07K14/415 , C07K2319/00 , C07K2319/02 , C12N9/1007 , C12N9/50 , C12N15/62 , C12N15/829
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which selectively disrupts the metabolism, functioning and/or development of cells of the flowers, particularly one or more of their female organs, or the seeds or the embryos of the plant. The foreign DNA sequence also optionally encodes a marker.

10. 发明申请

WO1991000353A2 MUTANT MICROBIAL 'alpha'-AMYLASES WITH INCREASED THERMAL, ACID AND/OR ALKALINE STABILITY 审中-公开
标题翻译：具有增加的热，酸和/或碱性稳定性的突变微生物'α' - 淀粉酶
公开(公告)号：WO1991000353A2
公开(公告)日：1991-01-10
申请号：PCT/EP1990001042
申请日：1990-06-27
申请人： GIST-BROCADES N.V. , PLANT GENETIC SYSTEMS N.V. , QUAX, Wilhelmus, Johannes , LAROCHE, Yves , VOLLEBREGT, Adrianus, Wilhelmus, Hermanus , STANSSENS, Patrick , LAUWEREYS, Marc
发明人： GIST-BROCADES N.V. , PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N15/56
CPC分类号： C12N15/75 , C12N9/2414 , C12N9/2417 , C12N15/70
摘要： Thermostable and acid stable α-amylases are provided as expression products of genetically engineered α-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant α-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.
摘要翻译：本发明涉及作为从微生物分离的基因工程α-淀粉酶基因表达产物的热稳定性和酸稳定性α-淀粉酶，所述微生物优选属于杆菌属（Bacilli）类。化学和酶促诱变的方法例如是酸性亚硫酸盐过程以及酶促的erronic掺入间隔的异源双链体DNA。突变α-淀粉酶在淀粉加工和纺织品退浆的工业应用中具有优异的性能，例如在宽pH范围内增强的热稳定性。

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