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1. 发明申请

WO1995034634A3 USE OF ANTHOCYANIN GENES TO MAINTAIN MALE STERILE PLANTS 审中-公开
公开(公告)号：WO1995034634A3
公开(公告)日：1995-12-21
申请号：PCT/EP1995002157
申请日：1995-06-06
申请人： PLANT GENETIC SYSTEMS, N.V. , KREBBERS, Enno , WILLIAMS, Mark , LEEMANS, Jan
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/82

2. 发明申请

WO1989003887A1 A PROCESS FOR THE PRODUCTION OF BIOLOGICALLY ACTIVE PEPTIDE VIA THE EXPRESSION OF MODIFIED STORAGE SEED PROTEIN GENES IN TRANSGENIC PLANTS 审中-公开
标题翻译：生物活性肽生产过程通过转基因植物修饰的储存种子蛋白基因的表达
公开(公告)号：WO1989003887A1
公开(公告)日：1989-05-05
申请号：PCT/EP1988000944
申请日：1988-10-20
申请人： PLANT GENETIC SYSTEMS N.V. , VANDEKERCKHOVE, Joël, Stefaan , KREBBERS, Enno , BOTTERMAN, Johan , LEEMANS, Jan
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12P21/02
CPC分类号： C12N15/8242 , C07K14/00 , C07K14/415 , C07K14/60 , C07K14/70 , C07K2319/02 , C12N15/8234 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12N15/8257
摘要： The invention pertains to a process for producing a determined polypeptide of interest or repeats thereof in a seed forming plant. It comprises: cultivating plants obtained from regenerated plant cells or from seeds of plants obtained from said regenerated plant cells over one or several generations, whose genetic patrimony, replicable with said plants, comprises a precursor-coding nucleic acid sequence encoding the precursor of a plant storage protein and placed under the control of a seed promoter, said precursor-coding nucleic acid being modified in a non-essential region of its relevant sequence which encodes the mature storage protein or a sub-unit thereof with a nucleic acid insert in appropriate reading phase relationship with the surrounding part of said relevant sequence, said insert including a determined segment encoding a heterologous determined polypeptide of interest or repeats thereof linked to each other and downstream and upstream thereof to the remainder parts of said relevant sequence through codons encoding aminoacid(s) which define selectively cleavable border sites surrounding the peptide of interest in the hybrid storage protein encoded by the so-modified relevant sequence of said precursor-coding nucleic acid; recovering the seeds of the cultivated plants and extracting the hybrid storage proteins contained therein; cleaving out the peptide of interest from said hybrid storage protein, purifying and recovering the peptide of interest. The polypeptide of interest may be a biologically active protein and/or a labeled protein.
摘要翻译：本发明涉及在种子形成植物中产生所确定的感兴趣的多肽或其重复序列的方法。它包括：从一个或几代的再生植物细胞或从所述再生植物细胞获得的植物种子中培养植物，其遗传遗传可与所述植物复制，其包含编码植物前体的前体编码核酸序列储存蛋白并置于种子启动子的控制之下，所述前体编码核酸在其相关序列的非必需区域中被修饰，所述相关序列编码成熟的存储蛋白或其亚单位，并以适当的读数与核酸插入片段与所述相关序列的周围部分的相位关系，所述插入物包含编码异源确定的感兴趣的多肽或其重复的确定的片段，其通过编码氨基酸（s）的密码子彼此连接并连接到所述相关序列的其余部分），其定义了围绕肽的选择性可切割的边界位点对由所述前体编码核酸的经修饰的相关序列编码的杂交储存蛋白的兴趣; 回收栽培植物的种子并提取其中所含的杂种储存蛋白; 从所述杂交储存蛋白中切出感兴趣的肽，纯化并回收目的肽。感兴趣的多肽可以是生物活性蛋白质和/或标记的蛋白质。

3. 发明申请

WO0060088A3 PLANT VIRAL MOVEMENT PROTEIN GENES 审中-公开
标题翻译：植物病毒运动蛋白基因
公开(公告)号：WO0060088A3
公开(公告)日：2001-04-26
申请号：PCT/US0009110
申请日：2000-04-06
申请人： DU PONT , KREBBERS ENNO , WENG ZUDE , CAHOON REBECCA E
发明人： KREBBERS ENNO , WENG ZUDE , CAHOON REBECCA E
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , C12Q1/68 , G01N33/68
CPC分类号： C12N15/8283 , C07K14/415 , C12N15/8209
摘要： This invention relates to an isolated nucleic acid fragment encoding a viral movement protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the viral movement protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the viral movement protein in a transformed host cell.
摘要翻译：本发明涉及编码病毒运动蛋白的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码病毒运动蛋白的全部或大部分的嵌合基因，其中嵌合基因的表达导致转化宿主中病毒运动蛋白水平的改变细胞。

4. 发明申请

WO1995034634A2 USE OF ANTHOCYANIN GENES TO MAINTAIN MALE STERILE PLANTS 审中-公开
标题翻译：使用蒽醌类基因维持雄性不育植物
公开(公告)号：WO1995034634A2
公开(公告)日：1995-12-21
申请号：PCT/EP1995002157
申请日：1995-06-06
申请人： PLANT GENETIC SYSTEMS, N.V. , KREBBERS, Enno , WILLIAMS, Mark , LEEMANS, Jan
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N00/00
CPC分类号： A01H1/02 , C07K14/415 , C12N15/8289 , Y10S47/01
摘要： A plant consisting essentially of cells which comprise in their genome a homozygous male-sterility genotype at a first genetic locus; and a color-linked restorer genotype at a second genetic locus, which is heterozygous (Rf/-) for a foreign DNA Rf. The foreign DNA Rf comprises: a) a fertility-restorer gene capable of preventing the phenotypic expression of the male-sterility genotype, and b) at least one anthocyanin regulatory gene involved in the regulation of anthocyanin biosynthesis in cells of seeds of the plant which is capable of producing anthocyanin at least in the seeds of the plant, so that anthocyanin production in the seeds is visible externally. Preferably, the anthocyanin regulatory gene is a shortened R, B or C1 gene or a continuation thereof. The invention also relates to DNA sequences encoding shortened R, B or C1 anthocyanin regulatory genes and to a process for maintaining a line of male-sterile plants which comprises crossing a male-sterile parent plant and a maintainer parent plant comprising homozygous male-sterility genotype and a restore genotype comprising fertility-restorer gene and an anthocyanin regulatory gene.
摘要翻译：基本上由细胞组成的植物，在其基因组中在第一遗传基因座处包含纯合的雄性不育基因型; 和在第二个遗传基因座上的染色连接的恢复基因型，其对于外源DNA Rf是杂合的（Rf / - ）。外源DNA Rf包括：a）能够预防雄性不育基因型表型表达的育性恢复基因，以及b）至少一种参与植物种子细胞中花青素生物合成调节的花青素调节基因，能够至少在植物的种子中产生花青素，使得种子中的花青素生产在外部是可见的。优选地，花青素调节基因是缩短的R，B或C1基因或其延续。本发明还涉及编码缩短的R，B或C1花青素调节基因的DNA序列以及保持一系列雄性不育植物的方法，该方法包括杂交雄性不育母本植物和包含纯合雄性不育基因型的维持母本植物以及包含生育恢复基因和花青素调节基因的恢复基因型。

5. 发明申请

WO2006138638A1 METHODS AND COMPOSITIONS FOR GENE SILENCING 审中-公开
标题翻译：用于基因沉默的方法和组合物
公开(公告)号：WO2006138638A1
公开(公告)日：2006-12-28
申请号：PCT/US2006/023607
申请日：2006-06-16
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E.I. DUPONT DE NEMOURS AND COMPANY , PLANT BIOSCIENCE LIMITED NORWICH (GB) , BAULCOMBE, David , KREBBERS, Enno , HITZ, William, D. , GLASSMAN, Kimberly, F. , AUKERMAN, Milo, J. , WILLIAMS, Robert , YOO, Byung-chun
发明人： BAULCOMBE, David , KREBBERS, Enno , HITZ, William, D. , GLASSMAN, Kimberly, F. , AUKERMAN, Milo, J. , WILLIAMS, Robert , YOO, Byung-chun
IPC分类号： C12N15/82 , C12N5/04 , A01H5/00 , A01H5/10 , A01H3/00
CPC分类号： C12N15/8218 , C07H21/02 , C07H21/04 , C12N2310/14
摘要： Methods and compositions are provided for reducing the level of expression of a target polynucleotide in an organism. The methods and compositions selectively silence the target polynucleotide through the expression of a chimeric polynucleotide comprising the target for a sRNA (the trigger sequence) operably linked to a sequence corresponding to all or part of the gene or genes to be silenced. In this manner, the final target of silencing is an endogenous gene in the organism in which the chimeric polynucleotide is expressed. In a further embodiment, the miRNA target is that of a heterologous miRNA or siRNA, the latter of which is coexpressed in the cells at the appropriate developmental stage to provide silencing of the final target when and where desired. In a further embodiment, the final target may be a gene in a second organism, such as a plant pest, that feeds upon the organism containing the chimeric gene or genes. Compositions further comprise vectors, seeds, grain, cells, and organisms, including plants and plant cells, comprising the chimeric polynucleotide of the invention.
摘要翻译：提供了用于降低生物体中靶多核苷酸表达水平的方法和组合物。所述方法和组合物通过表达嵌合多核苷酸来选择性沉默靶多核苷酸，所述嵌合多核苷酸包含可操作地连接到对应于待沉默的基因或基因的全部或部分的序列的sRNA（触发序列）的靶。以这种方式，沉默的最终靶标是其中表达嵌合多核苷酸的生物体中的内源基因。在另一个实施方案中，miRNA靶是异源miRNA或siRNA的靶标，其后者在合适的发育阶段在细胞中共表达，以在需要时和期望时提供最终靶的沉默。在另一个实施方案中，最终靶可以是在含有嵌合基因或基因的生物体上进食的第二生物体如植物害虫中的基因。组合物还包含载体，种子，谷粒，细胞和生物，包括植物和植物细胞，其包含本发明的嵌合多核苷酸。

6. 发明申请

WO1998016650A1 ENHANCED TRANSGENE EXPRESSION IN A POPULATION OF MONOCOT CELLS EMPLOYING SCAFFOLD ATTACHMENT REGIONS 审中-公开
标题翻译：增加移植区域的单克隆细胞群体的转录突变
公开(公告)号：WO1998016650A1
公开(公告)日：1998-04-23
申请号：PCT/US1997017709
申请日：1997-10-01
申请人： E.I. DU PONT DE NEMOURS AND COMPANY , ODELL, Joan, Tellefsen , KREBBERS, Enno
发明人： E.I. DU PONT DE NEMOURS AND COMPANY
IPC分类号： C12N15/82
CPC分类号： C12N15/822
摘要： A method of increasing transgene expression in a population of monocot plant cells is described which involves the use of a DNA construct comprising, inter alia , at least one chicken lysozyme gene locus scaffold attachment region.
摘要翻译：描述了在单子叶植物细胞群体中增加转基因表达的方法，其涉及使用包含尤其是至少一个鸡溶菌酶基因位点支架连接区的DNA构建体。

7. 发明申请

WO1990004032A1 A PROCESS FOR THE PRODUCTION OF TRANSGENIC PLANTS WITH INCREASED NUTRITIONAL VALUE VIA THE EXPRESSION OF MODIFIED 2S STORAGE ALBUMINS 审中-公开
标题翻译：通过修改的2S储存铝的表达生产具有增加的营养价值的转基因植物的方法
公开(公告)号：WO1990004032A1
公开(公告)日：1990-04-19
申请号：PCT/EP1989001229
申请日：1989-10-13
申请人： PLANT GENETIC SYSTEMS N.V. , EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA ... , DE CLERCQ, Ann , KREBBERS, Enno , VANDERKERCKHOVE, Joël , BARRETO DE CASTRO, Luiz , GANDER, Eugen , VAN MONTAGU, Marc
发明人： PLANT GENETIC SYSTEMS N.V. , EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA ...
IPC分类号： C12N15/82
CPC分类号： C07K14/00 , C07K14/415 , C07K2319/02 , C12N15/8251 , C12N15/8253 , C12N15/8254
摘要： The invention pertains to a process for producing transgenic plants with increased nutritional value. It comprises: cultivating plants obtained from regenerated plant cells or from seeds of plants obtained from said regenerated plant cells over one or several generations, whose genetic patrimony, replicable with said plants, comprises a precursor-coding nucleic acid sequence encoding the precursor of a 2S albumin storage protein and placed under the control of a promoter capable of directing gene expression in plants, said precursor-coding nucleic acid being modified in a nonessential region of its relevant sequence which encodes the mature 2S albumin or a subunit thereof with a nucleic acid insert in appropriate reading frame relationship with the surrounding part of said relevant sequence, said insert including a determined segment encoding an heterologous determined polypeptide containing appropriate aminoacid such as lysine and/or methionine and/or threonine and/or phenylalanine and/or trytophane and/or leucine and/or valine and/or isoleucine.
摘要翻译：本发明涉及生产营养价值增加的转基因植物的方法。它包括：从再生植物细胞获得的植物或从所述再生植物细胞获得的植物种子培育一代或几代，其遗传遗传可与所述植物复制，其包含编码2S前体的前体编码核酸序列白蛋白存储蛋白并置于能够引导植物中基因表达的启动子的控制下，所述前体编码核酸在其相关序列的非必需区域中被修饰，所述相关序列编码成熟的2S白蛋白或其亚基与核酸插入片段所述插入物包含编码含有适当氨基酸例如赖氨酸和/或甲硫氨酸和/或苏氨酸和/或苯丙氨酸和/或试ane酮的异源确定多肽的确定区段和/或亮氨酸和/或缬氨酸和/或异亮氨酸。

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