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1. 发明申请

WO1991016433A1 NEW BACILLUS THURINGIENSIS STRAINS AND THEIR GENES ENCODING INSECTICIDAL TOXINS 审中-公开
标题翻译：新型香草芽孢杆菌菌株及其编码杀虫毒素的基因
公开(公告)号：WO1991016433A1
公开(公告)日：1991-10-31
申请号：PCT/EP1991000791
申请日：1991-04-24
申请人： PLANT GENETIC SYSTEMS N.V. , PEFEROEN, Marnix , LAMBERT, Bart , VAN ANDENHOVE, Katrien
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N15/32
CPC分类号： C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162 , Y10S435/832
摘要： Two new Bacillus thuringiensis strains, which are deposited at the DSM under accession nos 5870 and 5871, produce new crystal proteins during sporulation that are toxic to Coleoptera and that are encoded by new genes. The crystal proteins contain protoxins, which can yield toxins as trypsin-digestion products. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes an insecticidally effective portion of its respective protoxin or encodes its respective toxin, is resistant to Coleoptera. Each strain, itself, or its crystals, crystal proteins, protoxin, toxin and/or insecticidally effective protoxin portion can be used as the active ingredient in an insecticidal composition for combatting Coleoptera.
摘要翻译：在DSM下保藏的两种新的苏云金芽孢杆菌菌株在加入5870和5871下，在孢子形成过程中产生新的晶体蛋白，对鞘翅目有毒，并由新基因编码。晶体蛋白含有原毒素，其可产生作为胰蛋白酶消化产物的毒素。一种植物，其基因组用来自任一种菌株的DNA序列转化并编码其各自原毒素的杀虫有效部分或编码其各自的毒素，对鞘翅目具有抗性。每种菌株本身或其晶体，晶体蛋白质，原毒素，毒素和/或杀虫有效的前毒素部分都可以用作杀虫鞘组合物中的活性成分。

2. 发明申请

WO1994005771A2 NEW BACILLUS THURINGIENSIS STRAINS AND THEIR INSECTICIDAL PROTEINS 审中-公开
标题翻译：新的BACILLUS THURINGIENSIS菌株及其杀虫蛋白
公开(公告)号：WO1994005771A2
公开(公告)日：1994-03-17
申请号：PCT/EP1993001820
申请日：1993-07-12
申请人： PLANT GENETIC SYSTEMS N.V. , LAMBERT, Bart , JANSENS, Stefan , VAN AUDENHOVE, Katrien , PEFEROEN, Marnix
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N01/20
CPC分类号： C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinia nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.
摘要翻译：四种新型苏云金芽孢杆菌菌株，其保藏在BCCM-LMG，保藏号为。 LMG P-12592，LMG P-12593，LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质，更特别地针对夜蛾科（Spodoptera spp）等等。和Agrotis ipsilon，针对鞭毛虫如鸵鸟（Ostrinia nubilalis），以及针对pon科（Yponomeutidae）如小菜蛾（Plutella xylostella），并且由新基因编码。晶体蛋白含有原毒素，其可以产生作为胰蛋白酶消化产物的毒素。一种植物，其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化，对鳞翅目具有抗性。每种菌株本身或其晶体，晶体蛋白质，原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。

3. 发明申请

WO1994024264A1 NEW BACILLUS THURINGIENSIS STRAINS AND THEIR INSECTICIDAL PROTEINS 审中-公开
标题翻译：新的BACILLUS THURINGIENSIS菌株及其杀虫蛋白
公开(公告)号：WO1994024264A1
公开(公告)日：1994-10-27
申请号：PCT/EP1994000553
申请日：1994-02-25
申请人： PLANT GENETIC SYSTEMS N.V. , LAMBERT, Bart , JANSENS, Stefan , VAN AUDENHOVE, Katrien , PEFEROEN, Marnix , VAN RIE, Jeroen , VAN AARSSEN, Roel
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N01/20
CPC分类号： C12R1/075 , A01N63/00 , C07K14/325 , C12N15/8286 , Y02A40/162 , Y10S424/832 , Y10S530/825
摘要： Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis spp., against Pyralidae such as Ostrinia nubilalis, against Gelechiidae such as Phthorimaea operculella, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystrals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.
摘要翻译：四种新型苏云金芽孢杆菌菌株，其保藏在BCCM-LMG，保藏号为。 LMG P-12592，LMG P-12593，LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质，更特别地针对夜蛾科（Spodoptera spp）等等。和Agrotis属，针对Pyr科（Ostrinia nubilalis），针对禾本科（Phleorimaea operculella）的Gelechiidae，以及针对小叶菜（Plutella xylostella）等Yponomeutidae，并由新基因编码。晶体蛋白含有原毒素，其可以产生作为胰蛋白酶消化产物的毒素。用来自任一种菌株并编码其各自毒素的DNA序列转化其基因组的植物对鳞翅目具有抗性。每种菌株本身或其低温物质，晶体蛋白质，原毒素或毒素均可用作杀虫鳞虫的杀虫组合物中的活性成分。

4. 发明申请

WO1991000353A2 MUTANT MICROBIAL 'alpha'-AMYLASES WITH INCREASED THERMAL, ACID AND/OR ALKALINE STABILITY 审中-公开
标题翻译：具有增加的热，酸和/或碱性稳定性的突变微生物'α' - 淀粉酶
公开(公告)号：WO1991000353A2
公开(公告)日：1991-01-10
申请号：PCT/EP1990001042
申请日：1990-06-27
申请人： GIST-BROCADES N.V. , PLANT GENETIC SYSTEMS N.V. , QUAX, Wilhelmus, Johannes , LAROCHE, Yves , VOLLEBREGT, Adrianus, Wilhelmus, Hermanus , STANSSENS, Patrick , LAUWEREYS, Marc
发明人： GIST-BROCADES N.V. , PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N15/56
CPC分类号： C12N15/75 , C12N9/2414 , C12N9/2417 , C12N15/70
摘要： Thermostable and acid stable α-amylases are provided as expression products of genetically engineered α-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant α-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.
摘要翻译：本发明涉及作为从微生物分离的基因工程α-淀粉酶基因表达产物的热稳定性和酸稳定性α-淀粉酶，所述微生物优选属于杆菌属（Bacilli）类。化学和酶促诱变的方法例如是酸性亚硫酸盐过程以及酶促的erronic掺入间隔的异源双链体DNA。突变α-淀粉酶在淀粉加工和纺织品退浆的工业应用中具有优异的性能，例如在宽pH范围内增强的热稳定性。

5. 发明申请

WO1987005629A1 PLANT CELLS RESISTANT TO GLUTAMINE-SYNTHETASE INHIBITORS, MADE BY GENETIC ENGINEERING 审中-公开
标题翻译：植物细胞抗谷氨酰胺合成酶抑制剂，通过遗传工程制造
公开(公告)号：WO1987005629A1
公开(公告)日：1987-09-24
申请号：PCT/EP1987000141
申请日：1987-03-11
申请人： PLANT GENETIC SYSTEMS N.V. , BIOGEN N.V. , LEEMANS, Jan , ROTTERMAN, Johan , DE BLOCK, Marc , THOMPSON, Charles , MOUVA, Rao
发明人： PLANT GENETIC SYSTEMS N.V. , BIOGEN N.V.
IPC分类号： C12N15/00
CPC分类号： A01H1/00 , C07K2319/00 , C12N9/1029 , C12N15/63 , C12N15/70 , C12N15/76 , C12N15/8277 , Y10S47/01
摘要： DNA fragment containing a determined gene, the expression of which inhibits the antibiotic and herbicidal effects of Bialaphos and related products. It also relates to recombinant vectors, containing such DNA fragment, which enable this protective gene to be introduced and expressed into cells and plant cells.

6. 发明申请

WO1991016432A1 MODIFIED BACILLUS THURINGIENSIS INSECTICIDAL-CRYSTAL PROTEIN GENES AND THEIR EXPRESSION IN PLANT CELLS 审中-公开
标题翻译：改良的BACILLUS THURINGIENSIS INSECTICIDAL-CRYSTAL蛋白基因及其在植物细胞中的表达
公开(公告)号：WO1991016432A1
公开(公告)日：1991-10-31
申请号：PCT/EP1991000733
申请日：1991-04-17
申请人： PLANT GENETIC SYSTEMS N.V. , CORNELISSEN, Marc , SOETAERT, Piet , STAM, Maike , DOCKX, Jan
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N15/31
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： A DNA fragment, encoding all or an insecticidally-effective part of a Bt crystal protein, is modified by changing A and T sequences to corresponding G and C sequences encoding the same amino acids.
摘要翻译：通过将A和T序列改变成编码相同氨基酸的相应G和C序列来修饰编码Bt结晶蛋白的全部或杀虫有效部分的DNA片段。

7. 发明申请

WO1990004032A1 A PROCESS FOR THE PRODUCTION OF TRANSGENIC PLANTS WITH INCREASED NUTRITIONAL VALUE VIA THE EXPRESSION OF MODIFIED 2S STORAGE ALBUMINS 审中-公开
标题翻译：通过修改的2S储存铝的表达生产具有增加的营养价值的转基因植物的方法
公开(公告)号：WO1990004032A1
公开(公告)日：1990-04-19
申请号：PCT/EP1989001229
申请日：1989-10-13
申请人： PLANT GENETIC SYSTEMS N.V. , EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA ... , DE CLERCQ, Ann , KREBBERS, Enno , VANDERKERCKHOVE, Joël , BARRETO DE CASTRO, Luiz , GANDER, Eugen , VAN MONTAGU, Marc
发明人： PLANT GENETIC SYSTEMS N.V. , EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA ...
IPC分类号： C12N15/82
CPC分类号： C07K14/00 , C07K14/415 , C07K2319/02 , C12N15/8251 , C12N15/8253 , C12N15/8254
摘要： The invention pertains to a process for producing transgenic plants with increased nutritional value. It comprises: cultivating plants obtained from regenerated plant cells or from seeds of plants obtained from said regenerated plant cells over one or several generations, whose genetic patrimony, replicable with said plants, comprises a precursor-coding nucleic acid sequence encoding the precursor of a 2S albumin storage protein and placed under the control of a promoter capable of directing gene expression in plants, said precursor-coding nucleic acid being modified in a nonessential region of its relevant sequence which encodes the mature 2S albumin or a subunit thereof with a nucleic acid insert in appropriate reading frame relationship with the surrounding part of said relevant sequence, said insert including a determined segment encoding an heterologous determined polypeptide containing appropriate aminoacid such as lysine and/or methionine and/or threonine and/or phenylalanine and/or trytophane and/or leucine and/or valine and/or isoleucine.
摘要翻译：本发明涉及生产营养价值增加的转基因植物的方法。它包括：从再生植物细胞获得的植物或从所述再生植物细胞获得的植物种子培育一代或几代，其遗传遗传可与所述植物复制，其包含编码2S前体的前体编码核酸序列白蛋白存储蛋白并置于能够引导植物中基因表达的启动子的控制下，所述前体编码核酸在其相关序列的非必需区域中被修饰，所述相关序列编码成熟的2S白蛋白或其亚基与核酸插入片段所述插入物包含编码含有适当氨基酸例如赖氨酸和/或甲硫氨酸和/或苏氨酸和/或苯丙氨酸和/或试ane酮的异源确定多肽的确定区段和/或亮氨酸和/或缬氨酸和/或异亮氨酸。

8. 发明申请

WO1989010396A1 PLANTS WITH MODIFIED STAMEN CELLS 审中-公开
标题翻译：具有修饰的STAMEN细胞的植物
公开(公告)号：WO1989010396A1
公开(公告)日：1989-11-02
申请号：PCT/EP1989000495
申请日：1989-04-27
申请人： PLANT GENETIC SYSTEMS N.V. , MARIANI, Celestina , LEEMANS, Jan , DE GREEF, Willy , DE BEUCKELEER, Marc
发明人： PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N05/00
CPC分类号： C12Y302/01031 , C07K14/415 , C12N9/1085 , C12N9/16 , C12N9/22 , C12N9/2434 , C12N9/50 , C12N15/8222 , C12N15/8231 , C12N15/8289 , C12Y301/27003
摘要： A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which selectively disrupts the metabolism, functioning and/or development of stamen cells of the plant. The foreign DNA sequence also optionally encodes a marker.

9. 发明申请

WO1990000605A1 METHODS AND MEANS FOR CONTROLLING THE STABILITY OF PROTEINS 审中-公开
标题翻译：控制蛋白质稳定性的方法和手段
公开(公告)号：WO1990000605A1
公开(公告)日：1990-01-25
申请号：PCT/EP1989000838
申请日：1989-07-17
申请人： PLANT GENETIC SYSTEMS N.V. , GIST - BROCADES N.V. , MRABET, Nadir , LASTERS, Ignace , STANSSENS, Patrick , MATTHYSSENS, Gaston , WODAK, Shoshana , QUAX, Wilhelmus, Johannes
发明人： PLANT GENETIC SYSTEMS N.V. , GIST - BROCADES N.V.
IPC分类号： C12N15/00
CPC分类号： C12Y102/01012 , C12N9/0008 , C12N9/0089 , C12N9/92 , C12P21/02
摘要： The invention pertains to a method for the production of a biologically active modified protein derived from a starting protein having essentially the same kind of biological activity with an attendant modulation effect on, particularly increase of, the stability as compared with that of the starting protein. The method comprises substituting an arginine residue for a lysine residue of the starting protein at a site that can sterically accommodate the substitution, without substantially altering the biological activity of the starting protein, said site being preferably of low solvent accessibility, at interfaces between domains or sub-units of the starting protein.
摘要翻译：本发明涉及一种生产生物活性的修饰蛋白质的方法，该蛋白质具有与起始蛋白质相比具有基本上相同种类的生物活性的起始蛋白质，具有调节作用，特别是增加的稳定性。该方法包括在可以空间适应取代的位置处将起始蛋白质的赖氨酸残基取代，而基本上不改变起始蛋白质的生物活性，所述位点优选在溶剂可接近性低的区域之间，起始蛋白质的亚单位。

10. 发明申请

WO1990000601A1 NOVEL GLUCOSE ISOMERASE ENZYMES AND THEIR USE 审中-公开
标题翻译：新型葡萄糖异构酶及其用途
公开(公告)号：WO1990000601A1
公开(公告)日：1990-01-25
申请号：PCT/EP1989000839
申请日：1989-07-17
申请人： GIST-BROCADES N.V. , PLANT GENETIC SYSTEMS N.V. , LUITEN, Rudolf, Gijsbertus, Marie , QUAX, Wilhelmus, Johannes , SCHUURHUIZEN, Paul, William , MRABET, Nadir
发明人： GIST-BROCADES N.V. , PLANT GENETIC SYSTEMS N.V.
IPC分类号： C12N09/92

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