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1. 发明申请

WO2004001063A3 METHODS AND COMPOSITIONS FOR MONITORING PRIMER EXTENSION AND POLYMORPHISM DETECTION REACTIONS 审中-公开
标题翻译：用于监测预处理延伸和多态性检测反应的方法和组合物
公开(公告)号：WO2004001063A3
公开(公告)日：2004-07-01
申请号：PCT/US0319699
申请日：2003-06-23
申请人： ORCHID BIOSCIENCES INC
发明人： MCKEOWN BRIAN
IPC分类号： C12N15/09 , C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6858 , C12Q2537/143 , C12Q2535/125 , C12Q2545/101
摘要： The methods of invention include the use of control primers to monitor the efficacy of amplification and/or primer extension reactions, and possible subsequent use of these control products as sizing markers. The methods of the invention are applicable to single reactions as well as to high-throughput and multiplex systems, including array-based technologies. One embodiment of the invention comprises monitoring the efficacy of a reaction for the detection of polymorphisms in the scrapie gene.
摘要翻译：本发明的方法包括使用对照引物来监测扩增和/或引物延伸反应的功效，以及随后将这些对照产物用作施胶标记物。本发明的方法适用于单反应以及高通量和多重系统，包括基于阵列的技术。本发明的一个实施方案包括监测用于检测瘙痒病基因中多态性的反应的功效。

2. 发明申请

WO0160519B1 WELL PLATE HOLDER 审中-公开
标题翻译：井板架
公开(公告)号：WO0160519B1
公开(公告)日：2002-02-07
申请号：PCT/US0140110
申请日：2001-02-14
申请人： ORCHID BIOSCIENCES INC
发明人： BONGARD RENE , TOKIWA GEORGE , RAYCA KATHRYN E , SABOL THOMAS , PETERSEN JAMES
IPC分类号： B01L3/00 , G01N35/02 , G01N35/04 , B01L9/00
CPC分类号： B01L3/5085 , B01L2300/021 , G01N35/028 , G01N2035/042
摘要： A well plate holder (10) has a generally planar bottom portion (24) having a first side, a second side, a third side and a fourth side. The first side has a first side wall portion (26) extending therefrom and the second side having a second side wall portion (26) extending therefrom. The third side may have a third side wall and the fourth side has a fourth side wall portion (26) extending therefrom. The first side wall may comprise dowel pins (28) and the second side wall may comprise a holding pin (30).
摘要翻译：孔板保持器（10）具有大致平坦的底部（24），其具有第一侧面，第二侧面，第三侧面和第四侧面。第一侧具有从其延伸的第一侧壁部分（26），第二侧部具有从其延伸的第二侧壁部分（26）。第三侧可具有第三侧壁，第四侧具有从其延伸的第四侧壁部分（26）。第一侧壁可以包括定位销（28），并且第二侧壁可以包括保持销（30）。

3. 发明申请

WO0076662A2 MICROENABLED CHEMICAL REACTION IN MICROFLUIDIC CHIPS 审中-公开
标题翻译：微流化学药剂中微量化学反应
公开(公告)号：WO0076662A2
公开(公告)日：2000-12-21
申请号：PCT/US0016056
申请日：2000-06-12
申请人： ORCHID BIOSCIENCES INC
发明人： SWENSON ROLF E , JIAN LIN , DEWITT SHEILA H
IPC分类号： B01J19/00 , B01L3/00 , C07B61/00 , C07C45/50 , C07C205/58 , C07C227/08 , C07C229/60 , C07K1/04 , C40B40/10 , C40B50/14 , C40B60/14 , B01L
CPC分类号： C07K1/047 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00319 , B01J2219/00351 , B01J2219/00353 , B01J2219/00418 , B01J2219/00454 , B01J2219/00459 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/0072 , B01J2219/00725 , B01L3/5027 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , B01L2300/0867 , B01L2300/0877 , B01L2400/0487 , C07B2200/11 , C07C45/50 , C07C201/12 , C07C227/08 , C40B40/10 , C40B50/14 , C40B60/14 , C07C229/60 , C07C205/58 , C07C47/02
摘要： A chemical process to obtain products of higher purity or in shorter time is provided by performing chemical reactions in a reactions volume less than 1 microliter. The advantages of this process are due to better mixing or heat exchanche (each 10 fold decrease in reaction volume leads to a 100-fold improvement mixing or heat exchange) or better surface to volume ratio. Better surface to volume ratio is a major factor in reactions critically dependent on surface mediated events such as heterogeneous catalysis. This increase in yield and speed of reaction can be conducted in parallel sets of reaction chambers provided by multilayer glass and silicon chips. Reagents can be added via a microfluidic chip incorporating channels and orthogonal row/column reagent delivery options. This discovery is amendable to solid supported synthesis as well as solution synthesis. Because of the better surface to volume ratio, this process is particularly useful with solid catalyst on the edges of the reaction chamber and can be used for hydrogenation, and hydroformulation. This process is demonstrated in reactions involving nucleophilic aromatic substitution on a solid support.
摘要翻译：通过在小于1微升的反应体积中进行化学反应来提供获得更高纯度或更短时间的产物的化学方法。该方法的优点是由于更好的混合或热交换（反应体积的每10倍减少导致混合或热交换改善100倍）或更好的表面与体积比。更好的表面与体积比是重要依赖于表面介导的事件（如非均相催化）的反应的主要因素。反应的产率和速度的这种增加可以由多层玻璃和硅片提供的平行的反应室组进行。可以通过结合通道和正交行/列试剂递送选项的微流控芯片添加试剂。这一发现可以修改为固体支持的合成以及溶液合成。由于表面与体积之比更好，该方法对于反应室边缘的固体催化剂特别有用，可用于氢化和加氢成型。在固体支持物上涉及亲核芳香取代的反应中证明了这一过程。

4. 发明申请

WO2004013346A2 METHODS AND COMPOSITIONS FOR GENOTYPING 审中-公开
标题翻译：基因组学的方法和组合
公开(公告)号：WO2004013346A2
公开(公告)日：2004-02-12
申请号：PCT/US0324198
申请日：2003-08-01
申请人： ORCHID BIOSCIENCES INC
发明人： MCKEOWN BRIAN , DERBYSHIRE ROGER , ROWAN PAUL , SUNG ROBERT
IPC分类号： C12Q1/68 , C12Q
CPC分类号： C12Q1/6858 , C12Q2545/101 , C12Q2525/155
摘要： The invention provides compositions and methods for performing primer extension reactions, including employment of amplification primers having 5' tags to incorporate into amplicons variant nucleotides of interest from target nucleic acids at known ratios, with or without sequences surrounding the variant nucleotides of interest. The invention provides identifying the variant nucleotides generated from the target nucleic acid and generated from the 5' tags, comparing the results, evaluating the efficiency of the primer extension reactions, and monitoring the efficacy of such reactions. The invention accounts for DNA sequence and experimental variables that may affect efficiency of incorporation of nucleotides, and provides a reference point for the interpretation of polymorphisms. The invention also provides methods of breeding scrapie-resistant sheep populations.
摘要翻译：本发明提供了用于进行引物延伸反应的组合物和方法，包括使用具有5'标签的扩增引物，以具有或不具有围绕所关注的变体核苷酸的序列，以目标核酸以已知比例掺入感兴趣的扩增子变体核苷酸。本发明提供鉴定从靶核酸产生并从5'标签产生的变体核苷酸，比较结果，评估引物延伸反应的效率和监测这些反应的功效。本发明涉及可能影响核苷酸掺入效率的DNA序列和实验变量，并为多态性的解释提供参考。本发明还提供了育种抗瘟病羊种群的方法。

5. 发明申请

WO0129262A3 GENOTYPING REAGENTS, KITS AND METHODS OF USE THEREOF 审中-公开
标题翻译：基因组试剂，试剂盒及其使用方法
公开(公告)号：WO0129262A3
公开(公告)日：2002-12-27
申请号：PCT/US0028436
申请日：2000-10-13
申请人： ORCHID BIOSCIENCES INC , PICOULT-NEWBURG LESLIE , POHL MARK
发明人： PICOULT-NEWBURG LESLIE , POHL MARK
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q2600/156
摘要： The present invention provides oligonucleotides that can be used to determine the presence, absence or identity of a single nucleotide polymorphism (SNP), kits containing such oligonucleotides, and methods of genotyping a nucleic acid sample using such oligonucleotides.
摘要翻译：本发明提供可用于确定单核苷酸多态性（SNP）的存在，不存在或同一性的寡核苷酸，含有此类寡核苷酸的试剂盒，以及使用这种寡核苷酸对核酸样品进行基因分型的方法。

6. 发明申请

WO2004003220A3 METHODS AND COMPOSITION FOR ANALYZING COMPROMISED SAMPLES USING SNPS 审中-公开
公开(公告)号：WO2004003220A3
公开(公告)日：2004-01-08
申请号：PCT/US2003/020150
申请日：2003-06-26
申请人： ORCHID BIOSCIENCES, INC , GILES, Robert , BAISCH, Jeanine, M. , MCKEOWN, Brian , STOLOROW, Mark
发明人： GILES, Robert , BAISCH, Jeanine, M. , MCKEOWN, Brian , STOLOROW, Mark
IPC分类号： C07H21/04
摘要： The present invention provides methods and compositions for analyzing compromised nucleic acid samples. The present invention also includes methods of selecting panels and panels of single nucleotide polymorphisms that are selected so as to be outside of tandem repeat regions, and are not genetically linked.

7. 发明申请

WO0050871A9 MICROSTRUCTURES FOR USE IN BIOLOGICAL ASSAYS AND REACTIONS 审中-公开
标题翻译：显微结构用于生物分析和反应
公开(公告)号：WO0050871A9
公开(公告)日：2001-08-30
申请号：PCT/US0005320
申请日：2000-02-25
申请人： ORCHID BIOSCIENCES INC
发明人： DAPPRICH JOHANNES
IPC分类号： B01L3/00 , B29C65/48 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N21/03 , G01N15/06 , C12M3/00
CPC分类号： B01L3/502707 , B01J2219/00286 , B01J2219/00317 , B01J2219/00353 , B01J2219/00378 , B01J2219/00389 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00659 , B01J2219/00677 , B01J2219/00691 , B01J2219/00722 , B01J2219/00743 , B01L3/505 , B01L3/5085 , B01L2200/0689 , B01L2200/12 , B01L2300/0654 , B01L2300/0819 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2400/0481 , B29C65/483 , B29C66/54 , B29C66/543 , B29C66/71 , B29L2031/756 , B82Y30/00 , C12Q1/6834 , C40B40/06 , C40B60/14 , G01N21/03 , G01N2021/0321 , G01N2021/0325 , G01N2021/0346 , Y10T436/25 , Y10T436/2575 , B29K2083/00 , B29K2075/00 , B29K2027/06
摘要： The present invention relates to microstructures (11) and microstructure assemblies (10) which are fabricated from a cast or molded polymer material, such as polydimethylsiloxane (PDMS). The invention describes and claims methods and compositions from which a variety of microstructures (11) may be prepared. The microstructures (11) are particularly useful for use in assays and reactions relating to biological assays, including applications in medical diagnostics. Such assays and reactions include, but are no means limited to, polynucleotide sequencing and analysis, including analysis of single nucleotide polymorphisms, and for polynucleotide amplification such as PCR.
摘要翻译：本发明涉及由浇铸或模制的聚合物材料如聚二甲基硅氧烷（PDMS）制造的微结构（11）和微结构组件（10）。本发明描述并要求可制备各种微结构（11）的方法和组合物。微结构（11）特别适用于与生物测定有关的测定和反应，包括在医学诊断中的应用。此类测定和反应包括但不限于多核苷酸测序和分析，包括单核苷酸多态性的分析，以及多核苷酸扩增如PCR。

8. 发明申请

WO0104909B1 FLUID DELIVERY SYSTEM FOR A MICROFLUIDIC DEVICE USING A PRESSURE PULSE 审中-公开
标题翻译：使用压力脉冲的微流体装置的流体输送系统
公开(公告)号：WO0104909B1
公开(公告)日：2001-05-03
申请号：PCT/US0040277
申请日：2000-06-21
申请人： ORCHID BIOSCIENCES INC
发明人： MCBRIDE STERLING EDUARD
IPC分类号： B01J19/00 , B01L3/00 , B01L99/00 , C40B60/14 , G01N1/00 , G01N35/00 , G25B9/00 , C12M1/34 , G01D15/18 , G01N1/14
CPC分类号： G01N35/1095 , B01J19/0093 , B01J2219/00317 , B01J2219/00409 , B01J2219/00418 , B01J2219/00585 , B01J2219/00783 , B01J2219/00853 , B01J2219/00891 , B01L3/0293 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L2200/0605 , B01L2200/0673 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , B01L2400/0487 , B01L2400/0688 , C40B60/14 , G01N2035/00148
摘要： A microfluidic fluid delivery system includes a microfluidic device (16) having a fluid input. A fluid reservoir (14) is fluidically coupled to the fluid input. A gas delivery system has a pulse generator (34, 34') that generates an electric pulse. An electrically operated valve (15, 30) is coupled to the pulse generator (34, 34') and the gas pressure source. The valve (15, 30) controls the gas pressure pulse in response to the electric pulse. The gas pressure pulse displaces fluid from the fluid reservoir (14) into the plurality of capillaries.
摘要翻译：微流体输送系统包括具有流体输入的微流体装置（16）。流体储存器（14）流体耦合到流体输入端。气体输送系统具有产生电脉冲的脉冲发生器（34,34'）。电动阀（15,30）连接到脉冲发生器（34,34'）和气体压力源。阀（15,30）响应于电脉冲控制气体压力脉冲。气体压力脉冲将流体从流体储存器（14）移位到多个毛细管中。

9. 发明申请

WO03020950A3 METHODS AND COMPOSITIONS FOR BI-DIRECTIONAL POLYMORPHISM DETECTION 审中-公开
标题翻译：用于双向多态性检测的方法和组合物
公开(公告)号：WO03020950A3
公开(公告)日：2003-04-17
申请号：PCT/US0227262
申请日：2002-08-27
申请人： ORCHID BIOSCIENCES INC , KUNKEL MARK , GELFAND CRAIG
发明人： KUNKEL MARK , GELFAND CRAIG
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6827 , C12Q2563/107 , C12Q2535/125
摘要： The present invention provides methods and compositions for detecting polymorphic sites by employing bi-directional primer extension reactions. In one embodiment, the present invention provides methods and compositions that minimize cost of reagents, such as labeled nucleotides, and minimize the cost of detection instrumentation.
摘要翻译：本发明提供了通过使用双向引物延伸反应来检测多态性位点的方法和组合物。在一个实施方案中，本发明提供使试剂（例如标记的核苷酸）的成本最小化以及使检测仪器的成本最小化的方法和组合物。

10. 发明申请

WO2004013346A8 METHODS AND COMPOSITIONS FOR GENOTYPING 审中-公开
标题翻译：基因组学的方法和组合
公开(公告)号：WO2004013346A8
公开(公告)日：2007-08-30
申请号：PCT/US0324198
申请日：2003-08-01
申请人： ORCHID BIOSCIENCES INC
发明人： MCKEOWN BRIAN , DERBYSHIRE ROGER , ROWAN PAUL , SUNG ROBERT
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6858 , C12Q2545/101 , C12Q2525/155
摘要： The invention provides compositions and methods for performing primer extension reactions, including employment of amplification primers having 5' tags to incorporate into amplicons variant nucleotides of interest from target nucleic acids at known ratios, with or without sequences surrounding the variant nucleotides of interest. The invention provides identifying the variant nucleotides generated from the target nucleic acid and generated from the 5' tags, comparing the results, evaluating the efficiency of the primer extension reactions, and monitoring the efficacy of such reactions. The invention accounts for DNA sequence and experimental variables that may affect efficiency of incorporation of nucleotides, and provides a reference point for the interpretation of polymorphisms. The invention also provides methods of breeding scrapie-resistant sheep populations.
摘要翻译：本发明提供了用于进行引物延伸反应的组合物和方法，包括使用具有5'标签的扩增引物，以具有或不具有围绕所关注的变体核苷酸的序列，以目标核酸以已知比例掺入感兴趣的扩增子变体核苷酸。本发明提供鉴定从靶核酸产生并从5'标签产生的变体核苷酸，比较结果，评估引物延伸反应的效率和监测这些反应的功效。本发明涉及可能影响核苷酸掺入效率的DNA序列和实验变量，并为多态性的解释提供参考。本发明还提供了育种抗瘟病羊群体的方法。

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