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1. 发明申请

WO2012061307A1 PORTABLE DEVICE FOR EX VIVO STIMULATION OF WHOLE BLOOD 审中-公开
标题翻译：便携式装置用于全血淋巴细胞浸润
公开(公告)号：WO2012061307A1
公开(公告)日：2012-05-10
申请号：PCT/US2011/058624
申请日：2011-10-31
申请人： HITACHI CHEMICAL CO., LTD , HITACHI CHEMICAL RESEARCH CENTER, INC. , MITSUHASHI, Masato , MURAKAMI, Taku
发明人： MITSUHASHI, Masato , MURAKAMI, Taku
IPC分类号： A61B5/15 , A61K35/14
CPC分类号： A01N1/0284 , A01N1/0252 , A61B5/15003 , A61B5/150206 , A61B5/150305 , A61B5/150389 , A61B5/150755 , A61B5/153 , A61B5/154 , A61K35/14
摘要： Disclosed are methods, device kits, and systems for improved quantification of mRNA from whole blood. More particularly, the devices and kites related thereto are useful for the controlled and repeatable ex vivo stimulation of whole blood.
摘要翻译：公开了用于改进来自全血的mRNA定量的方法，装置试剂盒和系统。更具体地，与其相关的装置和风筝对于全血的受控和可重复的离体刺激是有用的。

2. 发明申请

WO1998019837A1 CUTTING WORK VEHICLE 审中-公开
标题翻译：切割工作车辆
公开(公告)号：WO1998019837A1
公开(公告)日：1998-05-14
申请号：PCT/JP1997004017
申请日：1997-11-05
申请人： KOMATSU LTD. , KIMURA, Keiji , MURAKAMI, Taku
发明人： KOMATSU LTD.
IPC分类号： B27B17/00
CPC分类号： B28D1/088 , B28D1/082
摘要： A cutting work vehicle capable of performing cutting work while ensuring stability. The vehicle cuts a work (5) with a saw (3) while grasping the work (5) with a grappler (4). The vehicle comprises a lower travelling body (1) and an upper revolving body (2), the saw (3) being provided on the lower travelling body (1), and the grappler (4) being provided on the upper revolving body (2).
摘要翻译：一种能够在确保稳定性的同时进行切割工作的切割作业车辆。车辆用抓斗（4）抓住工件（5）时，用锯（3）切割工件（5）。车辆包括下行进体（1）和上旋转体（2），锯（3）设置在下行进体（1）上，并且抓斗（4）设置在上旋转体（2））。

3. 发明申请

WO1997028459A1 VEHICLE WITH MILLIMETER-WAVE RADAR 审中-公开
标题翻译：车辆与米勒雷达雷达
公开(公告)号：WO1997028459A1
公开(公告)日：1997-08-07
申请号：PCT/JP1997000206
申请日：1997-01-29
申请人： KOMATSU LTD. , KITAHARA, Yasuo , YAMASAKI, Kunihiro , MURAKAMI, Taku
发明人： KOMATSU LTD.
IPC分类号： G01S13/91
CPC分类号： G01S13/931 , G01S7/414 , G01S13/34 , G01S2013/9339 , G01S2013/9342 , G01S2013/9346 , G01S2013/936 , G01S2013/9375 , G01S2013/9378 , G01S2013/9385
摘要： A vehicle with a millimeter-wave radar capable of accurately detecting a stationary object around the vehicle while positively accepting ground clatters. Accordingly, the vehicle is provided with a millimeter-wave transmitting/receiving antenna (2F) fixed thereto with the center (C) of an antenna beam directed to a road surface (5), and a discrimination element (6) for classifying detected objects (4) by discriminating between objects (4a) the distance (L) to which varies with a movement of the vehicle and objects (4b) the distance (L) to which does not vary with a movement of the vehicle.
摘要翻译：具有毫米波雷达的车辆能够准确地检测车辆周围的静止物体，同时积极地接受地面咔嗒声。因此，车辆设置有固定于其上的毫米波发送/接收天线（2F），天线波束的中心（C）指向路面（5），以及用于对检测到的物体进行分类的识别元件（6）（4）通过区分物体（4a）与车辆的运动和物体（4b）之间的距离（L）随着车辆的运动而不变的距离（L）。

4. 发明申请

WO1997012713A1 DIAMOND TOOL FOR CUTTING REINFORCED STRUCTURE AND METHOD OF CUTTING REINFORCED STRUCTURE 审中-公开
标题翻译：用于切割加强结构的金刚石工具和切削加强结构的方法
公开(公告)号：WO1997012713A1
公开(公告)日：1997-04-10
申请号：PCT/JP1996002842
申请日：1996-09-30
申请人： KOMATSU LTD. , HOSHINO, Akifumi , MURAKAMI, Taku
发明人： KOMATSU LTD.
IPC分类号： B23D61/18
CPC分类号： B23D61/185 , B28D1/025 , B28D1/12
摘要： A diamond tool for cutting a reinforced structure and a method of cutting a reinforced structure, wherein the consumption of diamond abrasive is reduced, so that a life of the diamond tool is extended. An abrasive layer (1) of a diamond tool (10) for cutting a reinforced structure comprises diamond particles (2) and graphite particles (3) which are bonded together with binder (4). A preferable graphite content in the abrasive layer is 20 to 30 volume percentage. Also, a method of cutting a reinforced structure comprises cutting a reinforced structure (70) while feeding a cutting liquid containing graphite particles near the contact area between the reinforced structure (70) and a diamond tool (20). A preferable graphite content in the cutting liquid is 2 to 20 percent by weight.
摘要翻译：用于切割加强结构的金刚石工具和切割加强结构的方法，其中金刚石磨料的消耗减少，使得金刚石工具的寿命延长。用于切割加强结构的金刚石工具（10）的研磨层（1）包括金刚石颗粒（2）和石墨颗粒（3），其与粘合剂（4）结合在一起。研磨层中优选的石墨含量为20〜30体积％。此外，切割加强结构的方法包括：在加强结构（70）和金刚石工具（20）之间的接触区域附近供给包含石墨颗粒的切割液体的同时切割加强结构（70）。切削液中优选的石墨含量为2〜20重量％。

5. 发明申请

WO1992017749A1 METHOD FOR SENSING PART WHERE ELECTROMAGNETIC CHARACTERISTICS ARE CHANGED, AND DEVICE THEREOF 审中-公开
标题翻译：用于感应电磁特性变化的部分的方法及其装置
公开(公告)号：WO1992017749A1
公开(公告)日：1992-10-15
申请号：PCT/JP1992000352
申请日：1992-03-24
申请人： KABUSHIKI KAISHA KOMATSU SEISAKUSHO , MURAKAMI, Taku
发明人： KABUSHIKI KAISHA KOMATSU SEISAKUSHO
IPC分类号： G01D05/245
CPC分类号： G01D5/142 , G01B7/003 , G01D5/147 , G01D5/2451 , G01P3/488
摘要： A method for sensing with a high accuracy a single part, at which electromagnetic characteristics of a member to be sensed are changed, and for obtaining the output whose waveform is of a narrow peak width, and the device thereof. For this purpose, four electromagnetic sensing elements (10A-10D) are so aligned that when the two elements (10A, 10B) are simultaneously in the positions just over a protruding part (16) where electromagnetic characteristics are changed, the other two elements (10C, 10D) are in the positions just over a recessed part (18). Further, the mutually opposing two arms of a bridge circuit (30) are connected with one of the electromagnetic sensing elements (10A, 10B) and one of the elements (10C, 10D) respectively. Thereby, a single output having a waveform corresponding to the part at which the electromagnetic characteristics are changed is sensed.
摘要翻译：一种用于高精度地感测要被感测的构件的电磁特性改变的单个部分以及用于获得其波形窄峰值的输出的装置及其装置。为此，四个电磁感测元件（10A-10D）被对齐，使得当两个元件（10A，10B）同时位于电磁特性改变的突出部分（16）上方的位置时，另外两个元件 10C，10D）位于刚好在凹部（18）上的位置。此外，桥接电路（30）的相互相对的两个臂分别与电磁感测元件（10A，10B）和元件（10C，10D）之一连接。因此，检测到具有与电磁特性被改变的部分对应的波形的单个输出。

6. 发明申请

WO2006074162A3 PRIMER GENERATION ROLLING CIRCLE AMPLIFICATION 审中-公开
标题翻译：主发电轧制圆盘放大
公开(公告)号：WO2006074162A3
公开(公告)日：2006-08-31
申请号：PCT/US2006000086
申请日：2006-01-04
申请人： HITACHI CHEMICAL CO LTD , HITACHI CHEMICAL RES CT INC , MURAKAMI TAKU
发明人： MURAKAMI TAKU
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2531/125
摘要： A method of amplifying a nucleic acid is provided which comprises: generating a first nucleic acid primer from a first nucleic acid sequence; combining the first nucleic acid primer with a first polymerase and a first circular nucleic acid probe, wherein the first circular nucleic acid probe contains at least one antisense sequence to a second nucleic acid sequence and at least one antisense sequence to the first nucleic acid primer; producing at least one repeat of a sequence copy of the first circular nucleic acid probe by rolling circle amplification using the first polymerase, wherein the sequence copy contains at least the second nucleic acid sequence; generating a second nucleic acid primer from the second nucleic acid sequence; combining the second nucleic acid primer with a second polymerase and a second circular nucleic acid probe, where the second circular nucleic acid probe contains at least one antisense sequence to the second nucleic acid primer; and producing at least one repeat of a sequence copy of the second circular nucleic acid probe by rolling circle amplification using the second polymerase. The method may be employed to detect molecules of interest such as nucleic acid sequences, DNA methylation, single nucleotide polymorphisms (SNP), proteins and posttranslational modifications. Furthermore, a ribbon probe is provided that comprises a circular nucleic acid probe and a nucleic acid lock probe, wherein: the nucleic acid lock probe contains at least a cleavable linker, and the circular nucleic acid probe and the lock probe are unable to dissociate without cleaving the cleavable linker.
摘要翻译：提供了扩增核酸的方法，其包括：从第一核酸序列产生第一核酸引物; 将第一核酸引物与第一聚合酶和第一环状核酸探针组合，其中所述第一环状核酸探针含有与第二核酸序列的至少一个反义序列和与第一核酸引物的至少一个反义序列; 通过使用第一聚合酶的滚环扩增产生第一环状核酸探针的序列拷贝的至少一个重复序列，其中序列拷贝至少包含第二核酸序列; 从第二核酸序列产生第二核酸引物; 将所述第二核酸引物与第二聚合酶和第二环形核酸探针组合，其中所述第二环形核酸探针含有与所述第二核酸引物至少一个反义序列; 并通过使用第二聚合酶的滚环扩增产生第二环状核酸探针的序列拷贝的至少一个重复序列。该方法可用于检测感兴趣的分子，如核酸序列，DNA甲基化，单核苷酸多态性（SNP），蛋白质和翻译后修饰。此外，提供了包含环状核酸探针和核酸锁定探针的带状探针，其中：核酸锁定探针至少含有可切割的接头，并且环状核酸探针和锁定探针不能解离切割可切割的接头。

7. 发明申请

WO0242500A3 APPARATUS AND METHOD FOR ELECTROPHORETIC MICROSPOT CONCENTRATION 审中-公开
标题翻译：电子显微镜浓度的装置和方法
公开(公告)号：WO0242500A3
公开(公告)日：2003-05-01
申请号：PCT/US0146035
申请日：2001-10-31
申请人： HITACHI CHEMICAL RES CT INC , HITACHI CHEMICAL CO LTD , MITSUHASHI MASATO , MURAKAMI TAKU , TAMURA TSURUKI , YOHDA MASAFUMI , OKOCHI MINA
发明人： MITSUHASHI MASATO , MURAKAMI TAKU , TAMURA TSURUKI , YOHDA MASAFUMI , OKOCHI MINA
IPC分类号： B01D19/04 , B01D61/00 , B01L3/00 , C02F1/44 , C12Q1/68 , G01N1/34 , G01N1/40 , G01N27/447
CPC分类号： G01N27/44704 , B01L3/5025 , B01L2300/0618 , B01L2300/0829 , B01L2400/0421 , G01N27/44756 , G01N2001/4016 , G01N2001/4038
摘要： A method and apparatus for concentrating dilute biomolecules in samples using electrophoresis is described. The method involves using the charged nature of the biomolecules to localize them to a microspot containing a dialysis membrane. The method and apparatus may also be used for identifying the presence of a biomolecule in a mixture or to quantitate the amount of a specific biomolecule in a mixture. The method and apparatus may also be used to produce a high throughput method of analysis and quantitation of a sample containing biomolecules.
摘要翻译：描述了使用电泳浓缩样品中的稀释生物分子的方法和装置。该方法包括使用生物分子的带电性质将其定位于含有透析膜的微管。该方法和装置也可用于鉴定混合物中生物分子的存在或定量混合物中特定生物分子的量。该方法和装置还可用于产生含有生物分子的样品的高通量分析和定量方法。

8. 发明申请

WO1996013703A1 LOAD SENSOR SUBSTRATE AND LOAD SENSOR 审中-公开
标题翻译：负载传感器基板和负载传感器
公开(公告)号：WO1996013703A1
公开(公告)日：1996-05-09
申请号：PCT/JP1995002189
申请日：1995-10-25
申请人： KOMATSU LTD. , NABEOKA, Kazuyuki , MURAKAMI, Taku , KITSUNAI, Masami
发明人： KOMATSU LTD.
IPC分类号： G01L01/22
CPC分类号： G01G3/141 , G01L1/2237 , G01L5/0004
摘要： A load sensor substrate and a load sensor having a simple structure and a high strength and a high measurement accuracy. Four small holes (21 to 24) are bored in a metallic disc (10) symmetrically with respect to orthogonal axes (L1, L2) whose intersection is the center (Oo) of the disc. Strain gauges (31 to 34) having sensitivity directions which are the directions of the orthogonal axes are disposed between the adjacent holes (21 to 24). A load sensor is produced by fixing a load sensor substrate (100g) in a recess (320) of a pin (300), sealing the upper part of the recess (320) by a plug (330) and integrating it with the pin (300).
摘要翻译：负载传感器基板和负载传感器，其结构简单，强度高，测量精度高。四个小孔（21至24）相对于其交点是圆盘的中心（Oo）的正交轴线（L1，L2）对称地在金属盘（10）中钻孔。具有作为正交轴方向的敏感度方向的应变计（31〜34）配置在相邻的孔（21〜24）之间。负载传感器通过将负载传感器基板（100g）固定在销（300）的凹部（320）中，通过插头（330）密封凹部（320）的上部并将其与销（ 300）。

9. 发明申请

WO2011090803A1 METHOD OF MULTI PATHOGEN DETECTION 审中-公开
标题翻译：多病原体检测方法
公开(公告)号：WO2011090803A1
公开(公告)日：2011-07-28
申请号：PCT/US2011/000124
申请日：2011-01-24
申请人： HITACHI CHEMICAL CO., LTD. , HITACHI CHEMICAL RESEARCH CENTER, INC. , MURAKAMI, Taku
发明人： MURAKAMI, Taku
IPC分类号： C12N1/20
CPC分类号： C12Q1/04
摘要： A culturing method includes culturing a sample having one or more species of target pathogens of a detection assay and competing microorganisms under oxygen-poor condition in a growth medium. The growth medium can be a non-selective medium. The oxygen-poor condition is effective to prevent competing microorganisms from suppressing growth of the target pathogens. This method can be used in combination with a sample preparation method for large volume particulate samples using highly porous filter material and porous spherical filter aid.
摘要翻译：培养方法包括在生长培养基中培养具有检测测定的一种或多种目标病原体的样品和在贫氧条件下的竞争性微生物。生长培养基可以是非选择性培养基。缺氧条件有效地防止竞争性微生物抑制靶病原体的生长。该方法可以与使用高度多孔过滤材料和多孔球形助滤剂的大体积颗粒样品的样品制备方法结合使用。

10. 发明申请

WO2006074162A2 PRIMER GENERATION ROLLING CIRCLE AMPLIFICATION 审中-公开
标题翻译：主要产生滚子圆柱放大
公开(公告)号：WO2006074162A2
公开(公告)日：2006-07-13
申请号：PCT/US2006/000086
申请日：2006-01-04
申请人： HITACHI CHEMICAL COMPANY, LTD. , HITACHI CHEMICAL RESEARCH CENTER, INC. , MURAKAMI, Taku
发明人： MURAKAMI, Taku
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2531/125
摘要： A method of amplifying a nucleic acid is provided which comprises: generating a first nucleic acid primer from a first nucleic acid sequence; combining the first nucleic acid primer with a first polymerase and a first circular nucleic acid probe, wherein the first circular nucleic acid probe contains at least one antisense sequence to a second nucleic acid sequence and at least one antisense sequence to the first nucleic acid primer; producing at least one repeat of a sequence copy of the first circular nucleic acid probe by rolling circle amplification using the first polymerase, wherein the sequence copy contains at least the second nucleic acid sequence; generating a second nucleic acid primer from the second nucleic acid sequence; combining the second nucleic acid primer with a second polymerase and a second circular nucleic acid probe, where the second circular nucleic acid probe contains at least one antisense sequence to the second nucleic acid primer; and producing at least one repeat of a sequence copy of the second circular nucleic acid probe by rolling circle amplification using the second polymerase. The method may be employed to detect molecules of interest such as nucleic acid sequences, DNA methylation, single nucleotide polymorphisms (SNP), proteins and posttranslational modifications. Furthermore, a ribbon probe is provided that comprises a circular nucleic acid probe and a nucleic acid lock probe, wherein: the nucleic acid lock probe contains at least a cleavable linker, and the circular nucleic acid probe and the lock probe are unable to dissociate without cleaving the cleavable linker.
摘要翻译：提供了扩增核酸的方法，其包括：从第一核酸序列产生第一核酸引物; 将第一核酸引物与第一聚合酶和第一环状核酸探针组合，其中第一环状核酸探针含有至少一个与第二核酸序列的反义序列和至少一个与第一核酸引物的反义序列; 通过使用所述第一聚合酶的滚环扩增产生所述第一环状核酸探针的序列拷贝的至少一个重复序列，其中所述序列拷贝至少含有所述第二核酸序列; 从所述第二核酸序列产生第二核酸引物; 将所述第二核酸引物与第二聚合酶和第二环状核酸探针组合，其中所述第二环状核酸探针含有至少一个与所述第二核酸引物的反义序列; 以及通过使用第二聚合酶的滚环扩增产生第二环状核酸探针的序列拷贝的至少一个重复。该方法可用于检测感兴趣的分子，例如核酸序列，DNA甲基化，单核苷酸多态性（SNP），蛋白质和翻译后修饰。此外，提供了带状探针，其包括圆形核酸探针和核酸锁定探针，其中：所述核酸锁定探针至少含有可切割的接头，并且所述环状核酸探针和所述锁定探针不能在没有切割可切割的接头。

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