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1. 发明申请

WO0069561A8 LIQUID METAL-HEATING APPARATUS FOR BIOLOGICAL/CHEMICAL SAMPLE 审中-公开
标题翻译：液体金属加热装置生物/化学样品
公开(公告)号：WO0069561A8
公开(公告)日：2001-05-31
申请号：PCT/US0013220
申请日：2000-05-12
申请人： HITACHI CHEMICAL RES CT INC , UNIV CALIFORNIA , MITSUHASHI MASATO , CHU CHARLES Y
发明人： MITSUHASHI MASATO , CHU CHARLES Y
IPC分类号： B01L7/00 , B01L7/02 , C12M1/02 , F28D15/00 , B01L3/00 , C12M1/34 , C12M1/36 , F28F7/00 , F28F27/02
CPC分类号： B01L7/5255 , B01L7/02 , B01L7/52 , B01L2300/12 , B01L2300/185 , F28D15/00 , Y10S435/911 , Y10S435/912
摘要： An apparatus (figure 8) for temperature control of biological/chemical samples employing liquid metal (83) is described. A gallium-indium alloy may be used to provide excellent temperature control. Methods of using liquid metal to provide temperature control for biological/chemical samples are also described. A preferred use for the described liquid metal-heating apparatus is to provide precise temperature control for polymerase chain reaction (PCR).
摘要翻译：描述了使用液态金属（83）的生物/化学样品的温度控制的装置（图8）。可以使用镓铟合金来提供优异的温度控制。还描述了使用液态金属为生物/化学样品提供温度控制的方法。所述液体金属加热装置的优选用途是为聚合酶链反应（PCR）提供精确的温度控制。

2. 发明申请

WO2012009464A3 DETECTION OF NUCLEIC ACIDS BY AGGLUTINATION 审中-公开
标题翻译：通过AGGLUTINATION检测核酸
公开(公告)号：WO2012009464A3
公开(公告)日：2014-03-27
申请号：PCT/US2011043898
申请日：2011-07-13
申请人： HITACHI CHEMICAL CO LTD , HITACHI CHEMICAL RES CT INC , YAMAMOTO CINDY
发明人： YAMAMOTO CINDY
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6844 , C12Q2600/178 , C12Q2545/114 , C12Q2565/113 , C12Q2565/519
摘要： Embodiments of the invention relate generally to methods detecting, quantifying, or purifying nucleic acids by way of agglutination reactions. Several embodiments amplify target nucleic acids while incorporating a label such as 5-methyl-cytosine into amplified product and detecting, quantifying, or purifying the product with latex beads coupled to antibody reactive to the 5-methyl-cytosine labeled nucleic acid. Several embodiments incorporate DNA labels into specifically designed primers in order to detect, quantify, or purify a product after agglutination.
摘要翻译：本发明的实施方案一般涉及通过凝集反应检测，定量或纯化核酸的方法。几个实施方案在将扩增产物中引入5-甲基胞嘧啶等标记物的同时扩增靶核酸，并用与5-甲基 - 胞嘧啶标记的核酸反应的抗体偶联的乳胶珠检测，定量或纯化产物。为了在凝集后检测，定量或纯化产物，几个实施方案将DNA标记物并入专门设计的引物中。

3. 发明申请

WO2007117611A3 ENHANCED T CELL RECEPTOR-MEDIATED TUMOR NECROSIS FACTOR SUPERFAMILY AND CHEMOKINE MRNA EXPRESSION IN PERIPHERAL BLOOD LEUKOCYTES IN PATIENTS WITH CROHN'S DISEASE 审中-公开
标题翻译：增强T细胞受体介导的肿瘤坏死因子超家族和CHEMOKINE在外周血白细胞介素的表达与患有慢性疾病的患者
公开(公告)号：WO2007117611A3
公开(公告)日：2008-08-07
申请号：PCT/US2007008597
申请日：2007-04-05
申请人： HITACHI CHEMICAL CO LTD , HITACHI CHEMICAL RES CT INC , CEDARS SINAI MEDICAL CENTER , MITSUHASHI MASATO , TARGAN STEPHAN R
发明人： MITSUHASHI MASATO , TARGAN STEPHAN R
IPC分类号： G01N33/53
CPC分类号： C12Q1/6883 , C12Q2600/106 , C12Q2600/136 , C12Q2600/158
摘要： A method is disclosed for determining whether a human having Crohn's disease is likely to respond to a therapy targeting a TNFSF member or a cytokine by measuring the level of certain mRNAs in response to a stimulus. A method of evaluating the effectiveness of a Crohn's disease therapy in a human is also disclosed. Furthermore, a method of screening compounds for use in the treatment of Crohn's disease is disclosed. A method of monitoring the disease state over time in Crohn's disease patients is also disclosed.
摘要翻译：公开了一种用于通过测量响应于刺激的某些mRNA的水平来确定克罗恩病患者是否可能对靶向TNFSF成员或细胞因子的治疗作出反应的方法。还公开了评估人类克罗恩病治疗有效性的方法。此外，公开了一种筛选用于治疗克罗恩病的化合物的方法。还公开了一种在克罗恩病患者中随时间监测疾病状态的方法。

4. 发明申请

WO2006045053A3 METHOD FOR TAILORING ADMINISTRATION OF DRUGS BY QUANTITATION OF MRNA 审中-公开
标题翻译：通过MRNA定量定量化药物的方法
公开(公告)号：WO2006045053A3
公开(公告)日：2007-05-18
申请号：PCT/US2005037925
申请日：2005-10-20
申请人： HITACHI CHEMICAL CO LTD , HITACHI CHEMICAL RES CT INC , MITSUHASHI MASATO
发明人： MITSUHASHI MASATO
IPC分类号： C12Q1/68
CPC分类号： G01N33/57426 , C12Q1/6886 , C12Q2600/158 , G01N2333/4724
摘要： The present invention discloses a method for tailoring drug protocols to individual patients based on the levels of marker mRNA measured in leukocytes after stimulation of whole blood of the patient with candidate drugs. A method of measuring a patient's responsiveness to a drug is disclosed that includes exposing whole blood of the patient to the drug for 7 hours or less; after the exposure, measuring the amount of an mRNA associated with an effect of the drug in blood cells; and identifying responsiveness to the drug based on the results of the measurement, wherein a change in the amount of the mRNA indicates the patient's responsiveness to the drug. The amount of mRNA measured in the blood cells may be compared with the level of mRNA present in the cells before exposure or with the level of mRNA present in cells exposed for the same amount of time to a control vehicle. Marker mRNAs useful in the present invention include mRNAs encoding the gene product of the p21, BAX, PUMA, NOXA, and IL-2 genes. The method may be employed for patients with, among other conditions, cancer or diseases or conditions requiring immunosuppression.
摘要翻译：本发明公开了一种基于在患有候选药物的患者的全血刺激后在白细胞中测量的标记物mRNA水平对个体患者定制药物方案的方法。公开了一种测量患者对药物的反应性的方法，其包括将患者的全血暴露于药物7小时以下; 在暴露后，测量与药物在血细胞中的作用相关的mRNA的量; 并且基于测量结果确定对药物的反应性，其中mRNA量的变化表示患者对药物的反应性。可以将在血细胞中测量的mRNA的量与暴露前细胞中存在的mRNA水平或存在于暴露于对照载体相同时间量的细胞中的mRNA水平进行比较。可用于本发明的标记mRNA包括编码p21，BAX，PUMA，NOXA和IL-2基因的基因产物的mRNA。该方法可用于患有除其他病症之外的患有癌症或需要免疫抑制的疾病或病症的患者。

5. 发明申请

WO2006133399A9 METHOD FOR PREDICTING IMMUNE RESPONSE TO NEOPLASTIC DISEASE BASED ON mRNA EXPRESSION PROFILE IN NEOPLASTIC CELLS AND STIMULATED LEUKOCYTES 审中-公开
标题翻译：基于神经细胞和刺激白血病细胞中mRNA表达谱的免疫应答预防神经病变的方法
公开(公告)号：WO2006133399A9
公开(公告)日：2007-03-01
申请号：PCT/US2006022427
申请日：2006-06-08
申请人： HITACHI CHEMICAL RES CT INC , HITACHI CHEMICAL CO LTD , MITSUHASHI MASATO
发明人： MITSUHASHI MASATO
IPC分类号： C12Q1/70 , G01N33/00 , G01N33/53
CPC分类号： G01N33/505 , C12Q1/6806 , C12Q1/6886 , C12Q2600/118 , C12Q2600/158 , G01N33/574
摘要： Tumor necrosis factor (TNF) is capable of inducing apoptosis by interacting with specific TNF receptors on the surface of cancer cells. Because multiple members of TNF ligand and receptor are present within each superfamily, over 300 different ligand-receptor combinations exist. Activated blood leukocytes produce TNF as part of the immune response to cancer, as well as producing chemokines to attract other leukocytes to the site. A method is disclosed of detecting significant induction of a variety of TNF superfamily subtype and chemokine mRNAs in blood leukocytes when whole blood is exposed to heat-aggregated IgG or anti-T cell receptor antibodies as a model of immune system interactions. Substantial individual-to-individual variation is observed in TNF subtypes and chemokines induced. Since peripheral blood leukocytes are the supply of anti-cancer immune cells, the quantitation of ex vivo inducibility of appropriate TNF ligands and chemokines in blood will be useful in individualized cancer immunotherapy. If the tumor mass is small, such as with early invisible metastatic lesions, appropriate TNF assaults may be sufficient to prevent relapse.
摘要翻译：肿瘤坏死因子（TNF）能够通过与癌细胞表面的特异性TNF受体相互作用诱导细胞凋亡。由于TNF配体和受体的多个成员存在于每个超家族中，所以存在超过300种不同的配体 - 受体组合。活化的血液白细胞产生TNF作为对癌症的免疫应答的一部分，以及产生趋化因子以吸引其他白细胞到该部位。公开了当全血暴露于作为免疫系统相互作用的模型的热聚集的IgG或抗T细胞受体抗体时，检测血白细胞中各种TNF超家族亚型和趋化因子mRNA的显着诱导的方法。在TNF亚型和趋化因子诱导中观察到显着的个体与个体差异。由于外周血白细胞是抗癌免疫细胞的供应，所以在个体化的癌症免疫治疗中定量适当的TNF配体和趋化因子在血液中的离体诱导性将是有用的。如果肿瘤质量小，如早期隐形转移性病变，适当的TNF攻击可能足以防止复发。

6. 发明申请

WO2006116708A2 METHOD OF MINIMIZING REAGENT CONSUMPTION IN MICROPLATE-BASED REACTIONS 审中-公开
标题翻译：最小化基于微波反应的试剂消耗的方法
公开(公告)号：WO2006116708A2
公开(公告)日：2006-11-02
申请号：PCT/US2006016321
申请日：2006-04-28
申请人： HITACHI CHEMICAL RES CT INC , HITACHI CHEMICAL CO LTD , MITSUHASHI MASATO , OGURA MIEKO
发明人： MITSUHASHI MASATO , OGURA MIEKO
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： B01L3/50853 , B01L2300/046 , B01L2300/0654 , C12Q1/6806 , Y10S436/809 , C12Q2527/137
摘要： A method is provided for performing a reaction, such as the synthesis of concentrated cDNA, in the wells of a microplate while minimizing the volume of the solution of reagents required to perform the reaction. In the method, a pestle is inserted into the well of a microplate to which a substance has been immobilized. A volume of reagent solution is introduced into the well that is insufficient to cover the portion of the well onto which the substance is immobilized. The insertion of the pestle displaces reagent solution and increases the surface area of the solution in contact with the portion of the well to which the substance has been immobilized when the pestle is inserted.
摘要翻译：提供了一种方法，用于在微量培养板的孔中进行诸如合成浓缩cDNA的反应，同时使进行反应所需的试剂溶液的体积最小化。在该方法中，将杵插入已经固定有物质的微孔板的孔中。将一定体积的试剂溶液引入孔中，其不足以覆盖固定有物质的孔的部分。当插入杵时，杵的插入取代试剂溶液并增加溶液的表面积，该表面积与物质固定的孔的部分接触。

7. 发明申请

WO2006074162A3 PRIMER GENERATION ROLLING CIRCLE AMPLIFICATION 审中-公开
标题翻译：主发电轧制圆盘放大
公开(公告)号：WO2006074162A3
公开(公告)日：2006-08-31
申请号：PCT/US2006000086
申请日：2006-01-04
申请人： HITACHI CHEMICAL CO LTD , HITACHI CHEMICAL RES CT INC , MURAKAMI TAKU
发明人： MURAKAMI TAKU
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2531/125
摘要： A method of amplifying a nucleic acid is provided which comprises: generating a first nucleic acid primer from a first nucleic acid sequence; combining the first nucleic acid primer with a first polymerase and a first circular nucleic acid probe, wherein the first circular nucleic acid probe contains at least one antisense sequence to a second nucleic acid sequence and at least one antisense sequence to the first nucleic acid primer; producing at least one repeat of a sequence copy of the first circular nucleic acid probe by rolling circle amplification using the first polymerase, wherein the sequence copy contains at least the second nucleic acid sequence; generating a second nucleic acid primer from the second nucleic acid sequence; combining the second nucleic acid primer with a second polymerase and a second circular nucleic acid probe, where the second circular nucleic acid probe contains at least one antisense sequence to the second nucleic acid primer; and producing at least one repeat of a sequence copy of the second circular nucleic acid probe by rolling circle amplification using the second polymerase. The method may be employed to detect molecules of interest such as nucleic acid sequences, DNA methylation, single nucleotide polymorphisms (SNP), proteins and posttranslational modifications. Furthermore, a ribbon probe is provided that comprises a circular nucleic acid probe and a nucleic acid lock probe, wherein: the nucleic acid lock probe contains at least a cleavable linker, and the circular nucleic acid probe and the lock probe are unable to dissociate without cleaving the cleavable linker.
摘要翻译：提供了扩增核酸的方法，其包括：从第一核酸序列产生第一核酸引物; 将第一核酸引物与第一聚合酶和第一环状核酸探针组合，其中所述第一环状核酸探针含有与第二核酸序列的至少一个反义序列和与第一核酸引物的至少一个反义序列; 通过使用第一聚合酶的滚环扩增产生第一环状核酸探针的序列拷贝的至少一个重复序列，其中序列拷贝至少包含第二核酸序列; 从第二核酸序列产生第二核酸引物; 将所述第二核酸引物与第二聚合酶和第二环形核酸探针组合，其中所述第二环形核酸探针含有与所述第二核酸引物至少一个反义序列; 并通过使用第二聚合酶的滚环扩增产生第二环状核酸探针的序列拷贝的至少一个重复序列。该方法可用于检测感兴趣的分子，如核酸序列，DNA甲基化，单核苷酸多态性（SNP），蛋白质和翻译后修饰。此外，提供了包含环状核酸探针和核酸锁定探针的带状探针，其中：核酸锁定探针至少含有可切割的接头，并且环状核酸探针和锁定探针不能解离切割可切割的接头。

8. 发明申请

WO0242500A3 APPARATUS AND METHOD FOR ELECTROPHORETIC MICROSPOT CONCENTRATION 审中-公开
标题翻译：电子显微镜浓度的装置和方法
公开(公告)号：WO0242500A3
公开(公告)日：2003-05-01
申请号：PCT/US0146035
申请日：2001-10-31
申请人： HITACHI CHEMICAL RES CT INC , HITACHI CHEMICAL CO LTD , MITSUHASHI MASATO , MURAKAMI TAKU , TAMURA TSURUKI , YOHDA MASAFUMI , OKOCHI MINA
发明人： MITSUHASHI MASATO , MURAKAMI TAKU , TAMURA TSURUKI , YOHDA MASAFUMI , OKOCHI MINA
IPC分类号： B01D19/04 , B01D61/00 , B01L3/00 , C02F1/44 , C12Q1/68 , G01N1/34 , G01N1/40 , G01N27/447
CPC分类号： G01N27/44704 , B01L3/5025 , B01L2300/0618 , B01L2300/0829 , B01L2400/0421 , G01N27/44756 , G01N2001/4016 , G01N2001/4038
摘要： A method and apparatus for concentrating dilute biomolecules in samples using electrophoresis is described. The method involves using the charged nature of the biomolecules to localize them to a microspot containing a dialysis membrane. The method and apparatus may also be used for identifying the presence of a biomolecule in a mixture or to quantitate the amount of a specific biomolecule in a mixture. The method and apparatus may also be used to produce a high throughput method of analysis and quantitation of a sample containing biomolecules.
摘要翻译：描述了使用电泳浓缩样品中的稀释生物分子的方法和装置。该方法包括使用生物分子的带电性质将其定位于含有透析膜的微管。该方法和装置也可用于鉴定混合物中生物分子的存在或定量混合物中特定生物分子的量。该方法和装置还可用于产生含有生物分子的样品的高通量分析和定量方法。

9. 发明申请

WO2011156734A3 METHOD OF CHARACTERIZING VASCULAR DISEASES 审中-公开
标题翻译：表征血管疾病的方法
公开(公告)号：WO2011156734A3
公开(公告)日：2012-03-01
申请号：PCT/US2011040015
申请日：2011-06-10
申请人： HITACHI CHEMICAL CO LTD , HITACHI CHEMICAL RES CT INC , MITSUHASHI MASATO
发明人： MITSUHASHI MASATO
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6883 , C12Q2600/158 , C12Q2600/178
摘要： Embodiments of the invention relate generally to methods of diagnosing diseases and measuring homeostatic states. In particular, the methods described here are used to characterize RNA from vesicles for expression of disease related markers. Embodiments of the invention also relate generally to the characterization of RNA by using sensitive techniques such as PCR to internally sample organ health using whole blood.
摘要翻译：本发明的实施方案一般涉及诊断疾病和测量内稳态的方法。特别地，本文描述的方法用于表征囊泡中的RNA以表达与疾病相关的标记物。本发明的实施方案一般还涉及通过使用诸如PCR的敏感技术来使用全血对内部采样器官健康进行RNA的表征。

10. 发明申请

WO2007084796A3 IONIC POLYMER DEVICES AND METHODS OF FABRICATING THE SAME 审中-公开
标题翻译：离子聚合物装置及其制造方法
公开(公告)号：WO2007084796A3
公开(公告)日：2008-07-17
申请号：PCT/US2007001853
申请日：2007-01-23
申请人： HITACHI CHEMICAL RES CT INC , HITACHI CHEMICAL CO LTD , WU YONGXIAN , LI YANGYANG
发明人： WU YONGXIAN , LI YANGYANG
IPC分类号： C08J5/20
CPC分类号： H01M4/8642 , C08J5/20 , F03G7/005 , H01B1/122
摘要： An embodiment provides an ionic polymer device comprising two extended electrode layers comprising a plurality of conductive particles, wherein the plurality of conductive particles form a concentration gradient in each of the two extended electrode layers, an ionic polymer dielectric layer between two extended electrode layers, and at least one conductive layer on outer surfaces of two extended electrode layers. Another embodiment provides an ionic polymer device comprising a polymer composite with a plurality of surface features on two opposite surfaces, and at least one conductive layer on each of said two opposite surfaces. One embodiment provides a method of making an ionic polymer device, comprising forming a partially cured polymer-metallic salt layer, reducing the metallic salt to form a plurality of metal particles, thereby forming a first extended electrode layer and a second extended electrode layer at and near opposite surfaces of the ionic polymer device.
摘要翻译：一个实施方案提供了一种离子聚合物装置，其包含两个包括多个导电颗粒的延伸电极层，其中多个导电颗粒在两个延伸电极层中的每一个中形成浓度梯度，在两个延伸电极层之间形成离子聚合物电介质层，在两个延伸的电极层的外表面上的至少一个导电层。另一个实施方案提供了一种离子聚合物装置，其包括在两个相对表面上具有多个表面特征的聚合物复合物，以及在所述两个相对表面中的每一个上的至少一个导电层。一个实施方案提供了一种制备离子聚合物装置的方法，包括形成部分固化的聚合物金属盐层，还原金属盐以形成多个金属颗粒，由此在其上形成第一延伸电极层和第二延伸电极层，靠近离子聚合物装置的相对表面。

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