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    • 2. 发明申请
    • AN ENZYME AND ENZYME PREPARATION WITH ENDOGLUCANASE ACTIVITY
    • 使用内切酶活性的酶和酶制备
    • WO1996011262A1
    • 1996-04-18
    • PCT/DK1995000400
    • 1995-10-06
    • NOVO NORDISK A/SSCHÜLEIN, MartinOXENBOLL, Karen, MargretheANDERSEN, Lene, NonboeLASSEN, Soren, FlenstedKAUPPINEN, Markus, SakariNIELSEN, Jack, Bech
    • NOVO NORDISK A/S
    • C12N09/42
    • D21H17/005C11D3/38645C12N9/2437C12N15/81C12Y302/01004D06M16/003D06P5/158D21C5/005D21H21/10
    • An enzyme composition with substantial cellulolytic, especially endoglucanase, activity at alkaline conditions which is derived from or producible by fungi from the genus Acremonium, provided that the enzyme composition is not immunologically cross-reactive with antibodies raised against endoglucanases (EC 3.2.1.4) from Humicola insolens, DSM 1800, Fusarium oxysporum, DSM 2672, Myceliophthora thermophila, CBS 117.65, or Cephalosporium sp., RYM-202. The invention further relates to a DNA construct comprising a DNA sequence encoding an enzyme exhibiting endoglucanase activity. The enzyme composition and the isolated or cloned and expressed cellulase of the invention may be useful in any industrial process requiring an alkaline cellulase, e.g. for providing localised variation in the colour density of dyed fabric such as stone-washing of denim, for improving the drainage of an aqueous suspension of paper pulp, for the de-inking of recycled paper, in detergent compositions and in fabric softeners.
    • 在碱性条件下具有显着的纤维素分解,特别是内切葡聚糖酶的酶组合物,其衍生自或者由来自顶孢菌属的真菌产生的活性,条件是所述酶组合物与针对内切葡聚糖酶(EC 3.2.1.4)产生的抗体不具有免疫交叉反应性 Humicola insolens,DSM 1800,尖孢镰刀菌,DSM 2672,嗜热丝孢杆菌,CBS 117.65或头孢菌属,RYM-202。 本发明还涉及包含编码表现出内切葡聚糖酶活性的酶的DNA序列的DNA构建体。 本发明的酶组合物和分离或克隆和表达的纤维素酶可用于任何需要碱性纤维素酶的工业过程,例如, 用于提供染色织物的颜色密度的局部变化,例如牛仔布的石洗,用于改进纸浆的水性悬浮液的排出,用于在再生纸中脱墨,在洗涤剂组合物和织物柔软剂中的排墨。