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1. 发明申请

WO2006054815A1 RECOMBINANT EXPRESSION VECTOR FOR PRODUCTION OF PLANTS HAVING MULTIPLE STRESS TOLERANCES, AND METHOD FOR PREPARING MULTIPLE STRESS-TOLERANT PLANTS USING THE SAME 审中-公开
标题翻译：用于生产具有多种应力耐力的植物的重组表达载体，以及使用该方法制备多种耐胁迫性植物的方法
公开(公告)号：WO2006054815A1
公开(公告)日：2006-05-26
申请号：PCT/KR2005/000258
申请日：2005-01-28
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , KWAK, Sang-Soo , KWON, Suk-Yoon , LEE, Haeng-Soon , TANG, Li , LIM, Soon , LEE, Byung-Hyun
发明人： KWAK, Sang-Soo , KWON, Suk-Yoon , LEE, Haeng-Soon , TANG, Li , LIM, Soon , LEE, Byung-Hyun
IPC分类号： C12N15/82
CPC分类号： C12N15/8273 , C12N15/8271
摘要： The present invention relates to a recombinant expression vector for production of multiple stress tolerant plants, which is prepared by attaching multiple stress tolerant genes to oxidative stress inducible promoter, multiple stress tolerant plants transformed with the expression vector and a preparation method for the plants, more precisely, a recombinant expression vector for production of multiple stress tolerant plants that is prepared by combining oxidative stress inducible peroxidase promoter (SWPA2) originated from sweetpotato with multiple stress tolerant genes (SOD (superoxide dismutase) and APX (ascobate peroxidase)) to express the multiple stress tolerant genes in chloroplasts, multiple stress tolerant plants transformed with the expression vector above and a preparation method for the plants. The recombinant expression vector of the present invention is very useful for the production of transformed plants having very strong resistance against multiple stresses caused by oxidative stress inducible herbicides, cold injury, high temperature, salt damage, or various environmental pollutions generating active oxygen, so that the vector can be a great contribution to the increase of productivity of agricultural crops or mass-production of useful components.
摘要翻译：本发明涉及用于产生多种胁迫耐受植物的重组表达载体，其通过将多个胁迫耐受基因连接到氧化应激诱导型启动子，用表达载体转化的多种胁迫耐受植物和植物的制备方法准确地说，通过将源自甘薯的氧化应激诱导型过氧化物酶启动子（SWPA2）与多重胁迫耐受基因（SOD（超氧化物歧化酶）和APX（ascobate过氧化物酶））组合来制备多重胁迫耐受植物的重组表达载体，以表达叶绿体中的多重胁迫耐受基因，用上述表达载体转化的多种胁迫耐受植物和植物的制备方法。本发明的重组表达载体对于生产对由氧化应激诱导型除草剂，冷伤害，高温，盐损害或产生活性氧的各种环境污染引起的多重胁迫具有非常强的抗性的转化植物的生产非常有用，使得该载体可以为提高农作物生产力或大量生产有用成分做出重大贡献。

2. 发明申请

WO2009066919A2 GPT GENE AS A MARKER FOR SELECTING PLANT TRANSFORMANT 审中-公开
标题翻译： GPT基因作为选择植物转化体的标记
公开(公告)号：WO2009066919A2
公开(公告)日：2009-05-28
申请号：PCT/KR2008006784
申请日：2008-11-18
申请人： KOREA RES INST OF BIOSCIENCE , JEON JAE HEUNG , KIM HYUN SOON , KIM YOON SIK , LEE YONG HWA , KIM MI SUN , MIN SUNG RAN , JEONG WON JOONG , KWON SUK YOON , JOUNG HYOUK
发明人： JEON JAE HEUNG , KIM HYUN SOON , KIM YOON SIK , LEE YONG HWA , KIM MI SUN , MIN SUNG RAN , JEONG WON JOONG , KWON SUK YOON , JOUNG HYOUK
IPC分类号： C12N15/11
CPC分类号： C12N9/1288 , C12N15/8209
摘要： The present invention relates to a marker consisting of GPT (UDP-?/-acetylgluco samine:dolichol phosphate-?/-acetylglucosamine-phosphotransferase) gene to select a transformed plant, a recombinant plant expression vector comprising GPT gene, plants that are transformed with said vector and seeds of the plants, a method of selecting a tr ansformed plant comprising steps of transforming a plant cell with said vector and selec ting a transformant in a medium comprising tunicamycin, and a method of producing a t ransformed plant comprising a step of transforming a plant cell with said vector.
摘要翻译：本发明涉及一种由GPT（UDP-β/ - 乙酰基葡糖胺：磷酸二氢叶酸磷酸酯-β/ - 乙酰葡糖胺磷酸转移酶）基因组成的基因，用于选择转化植物，包含GPT基因的重组植物表达载体，所述植物的载体和种子，选择转基因植物的方法，包括以下步骤：用所述载体转化植物细胞并在包含衣霉素的培养基中选择转化体，以及在转化植物中生产的方法，包括转化步骤具有所述载体的植物细胞。

3. 发明申请

WO2009054564A1 METHOD FOR THE SYNTHESIS OF NUCLEIC ACID BY USING FRAGMENTED NUCLEIC ACIDS AND N-PRIMER 审中-公开
标题翻译：通过使用裂解的核酸和N-PRIMER合成核酸的方法
公开(公告)号：WO2009054564A1
公开(公告)日：2009-04-30
申请号：PCT/KR2007/006070
申请日：2007-11-28
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , MOON, Jae Sun , KIM, Sung Uk , HWANG, In Gyu , JWA, Nam Soo , KWON, Suk Yoon
发明人： MOON, Jae Sun , KIM, Sung Uk , HWANG, In Gyu , JWA, Nam Soo , KWON, Suk Yoon
IPC分类号： C12N15/09 , C12N15/11 , C12Q1/68
CPC分类号： C12N15/1096 , C12N15/1093 , C12Q1/6844 , C12Q2523/301 , C12Q2521/107 , C12Q2525/179
摘要： The present invention relates to a method for the synthesis of nucleic acid based on RNA fragmentation and N-primer which is prepared by using a fragmented RNA as a template, a method for constructing a cDNA library, a cDNA library constructed by said method, and a kit comprising N-primer for the synthesis of nucleic acid. According to the present invention, N-primer which may have the same complementarity for all regions of mRNA and various kinds of nucleic acids including mRNAs that are randomly fragmented by an artificial method are used for the synthesis of cDNAs or strands of complementary nucleic acids for every region. Thus, an overlap can be avoided and all kinds of cDNAs originating from mRNA or base sequences of the nucleic aids that are complementary to mRNA can be obtained.
摘要翻译：本发明涉及一种基于RNA片段化的核酸的合成方法和通过使用片段化RNA作为模板制备的N-引物，构建cDNA文库的方法，通过所述方法构建的cDNA文库，以及包含用于合成核酸的N-引物的试剂盒。根据本发明，可以对mRNA的所有区域具有相同的互补性的N-引物和包括通过人造方法随机碎裂的mRNA的各种核酸用于合成互补核酸的cDNA或链，用于每个地区。因此，可以避免重叠，可以获得源自与mRNA互补的核酸辅助子的mRNA或碱基序列的各种cDNA。

4. 发明申请

WO2004003225A1 METHOD FOR HIGH THROUGHPUT SCREENING OF PLANT GROWTH REGULATOR 审中-公开
标题翻译：植物生长调节剂高通量筛选方法
公开(公告)号：WO2004003225A1
公开(公告)日：2004-01-08
申请号：PCT/KR2003/001041
申请日：2003-05-28
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , KWAK, Sang-Soo , LEE, Haeng-Soon , KWON, Suk-Yoon , KIM, Chang-Jin , LEE, Hyang-Burm , LEE, Sang-Han
发明人： KWAK, Sang-Soo , LEE, Haeng-Soon , KWON, Suk-Yoon , KIM, Chang-Jin , LEE, Hyang-Burm , LEE, Sang-Han
IPC分类号： C12Q1/02
CPC分类号： G01N33/5097 , G01N2430/20 , G01N2500/00
摘要： The present invention relates to a method for high throughput screening of plant growth regulator, more particularly to the method comprising; 1) culturing phytomixotrophic cells and candidates of plant growth regulator which were added in a microwell plate, 2) treating 2,3,5-triphenyltetrazolium chloride thereto, 3) reacting thereof by adding ethanol after removing solutions from microwells, 4) transferring the reacting solution into the new microwell plate, and 5) measuring optical density with a high throughput screening reader. Since the method of the present invention can rapidly and conveniently screen many samples and can also evaluate in vivo activities of plant growth regulators, it can effectively be used as a screening method for plant growth inhibitors and activators.
摘要翻译：本发明涉及一种用于植物生长调节剂高通量筛选的方法，更具体地涉及包括以下步骤的方法： 1）培养植物营养细胞和添加在微孔板中的植物生长调节剂候选物，2）处理2,3,5-三苯基四唑氯化物，3）通过在从微孔中除去溶液后加入乙醇进行反应，4）解决方案进入新的微孔板，以及5）用高通量筛选阅读器测量光密度。由于本发明的方法可以快速方便地筛选许多样品，还可以评估植物生长调节剂的体内活性，因此可有效地用作植物生长抑制剂和活化剂的筛选方法。

5. 发明申请

WO2009066919A3 GPT GENE AS A MARKER FOR SELECTING PLANT TRANSFORMANT 审中-公开
公开(公告)号：WO2009066919A3
公开(公告)日：2009-05-28
申请号：PCT/KR2008/006784
申请日：2008-11-18
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , JEON, Jae Heung , KIM, Hyun Soon , KIM, Yoon Sik , LEE, Yong Hwa , KIM, Mi Sun , MIN, Sung Ran , JEONG, Won Joong , KWON, Suk Yoon , JOUNG, Hyouk
发明人： JEON, Jae Heung , KIM, Hyun Soon , KIM, Yoon Sik , LEE, Yong Hwa , KIM, Mi Sun , MIN, Sung Ran , JEONG, Won Joong , KWON, Suk Yoon , JOUNG, Hyouk
IPC分类号： C12N15/11
摘要： The present invention relates to a marker consisting of GPT (UDP-Λ/-acetylgluco samine:dolichol phosphate-Λ/-acetylglucosamine-phosphotransferase) gene to select a transformed plant, a recombinant plant expression vector comprising GPT gene, plants that are transformed with said vector and seeds of the plants, a method of selecting a tr ansformed plant comprising steps of transforming a plant cell with said vector and selec ting a transformant in a medium comprising tunicamycin, and a method of producing a t ransformed plant comprising a step of transforming a plant cell with said vector.

6. 发明申请

WO2009051289A1 PATATIN-FREE POTATO PLANT 审中-公开
标题翻译： PATATIN-POTATO植物
公开(公告)号：WO2009051289A1
公开(公告)日：2009-04-23
申请号：PCT/KR2007/006078
申请日：2007-11-29
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , JEON, Jae Heung , KIM, Hyun Soon , KIM, Yoon Sik , LEE, Yong Hwa , KIM, Mi Sun , MIN, Sung Ran , JEONG, Won Joong , KWON, Suk Yoon , JOUNG, Hyouk
发明人： JEON, Jae Heung , KIM, Hyun Soon , KIM, Yoon Sik , LEE, Yong Hwa , KIM, Mi Sun , MIN, Sung Ran , JEONG, Won Joong , KWON, Suk Yoon , JOUNG, Hyouk
IPC分类号： C12N15/63 , C12N15/64 , C12N15/82 , A01H1/00
CPC分类号： C12N15/8218 , C12N15/8257
摘要： The present invention relates to a patatin-free potato plant. More specifically, the present invention relates to a recombinant vector comprising patatin gene that is ope ratively inserted to a vector for hairpin RNA interference (hpRNAi), a plant transformed with said vector, and a method of producing glycoproteins for treating conditions in hum ans comprising steps of expressing said glycoproteins in the above-described transgenic plant and isolating them therefrom. It is expected that the patatin-free or patatin-reduced potato plant of the present invention that is transformed with hpRNAi can be used as a host for producing various human therapeutic glycoproteins, due to plant-specific g lycosylation and reduction in glycoproteins.
摘要翻译：本发明涉及一种不含patatin的马铃薯植物。更具体地说，本发明涉及包含patatin基因的重组载体，该基因插入到发夹RNA干扰载体（hpRNAi），用所述载体转化的植物，以及生产糖蛋白的方法，所述方法包括：在上述转基因植物中表达所述糖蛋白并从其中分离的步骤。由于植物特异性g赖氨酰化和糖蛋白的还原，预期用hpRNAi转化的本发明的不含patatin的或patatin-reduced马铃薯植物可用作产生各种人类治疗性糖蛋白的宿主。

7. 发明申请

WO2009031740A1 FUNCTIONAL DATA INTEGRATED GENETIC MARKER SYSTEM, SSR-FMM, AND IMPROVED GENETIC RESEARCH AND MARKER ASSISTED SELECTION WITH SSR-FMM 审中-公开
标题翻译：功能数据综合遗传标记系统，SSR-FMM和改良的遗传研究和标记辅助选择与SSR-FMM
公开(公告)号：WO2009031740A1
公开(公告)日：2009-03-12
申请号：PCT/KR2008/001478
申请日：2008-03-17
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , HUR, Cheol Goo , PAIK, Hyo Jung , KWON, Suk Yoon , CHOI, Do Il , KIM, Byung Dong , HAN, Jung Heon , PARK, Jeong Mee
发明人： HUR, Cheol Goo , PAIK, Hyo Jung , KWON, Suk Yoon , CHOI, Do Il , KIM, Byung Dong , HAN, Jung Heon , PARK, Jeong Mee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895
摘要： The present invention relates to an integrated genetic marker system SSR-FMM comprising a consensus sequence in which SSR (Simple Sequence Repeat), that is present in a consensus sequence produced by EST clustering and contig assembly and entire mRNA sequence or a part of RNA transcript sequence, and a domain sequence pattern FM (Functional Motif), that is present when translated into a protein sequence, are comprised, or a part of transcript, or an entire marker. The SSR-FMM genetic marker system of the present invention can be provided as a molecular genetic marker system that can be efficiently used for simultaneous implementation of trait selection and functional analysis of a gene by an integrative analysis of genetic information and -omics information that is required for identifying a trait function.
摘要翻译：本发明涉及一种综合遗传标记系统SSR-FMM，其包含共有序列，其中存在于通过EST聚类和重叠群组合产生的共有序列中的SSR（简单序列重复）和整个mRNA序列或RNA转录物的一部分序列，以及当翻译成蛋白质序列时存在的域序列模式FM（Functional Motif），其包含或部分转录物或整个标记。可以提供本发明的SSR-FMM遗传标记系统作为分子遗传标记系统，其可以有效地用于同时实现基因的性状选择和功能分析，通过遗传信息和 - 组学信息的综合分析需要识别特征功能。

8. 发明申请

WO2005080557A1 MULTIPLE STRESS-INDUCIBLE PEROXIDASE PROMOTER DERIVED FROM IPOMOEA BATATAS 审中-公开
标题翻译：来自IPOMOEA BATATAS的多重应力诱导过氧化物酶促进剂
公开(公告)号：WO2005080557A1
公开(公告)日：2005-09-01
申请号：PCT/KR2004/000381
申请日：2004-02-24
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , KWAK, Sang-Soo , KWON, Suk-Yoon , LEE, Haeng-Soon , RYU, Sun-Hwa
发明人： KWAK, Sang-Soo , KWON, Suk-Yoon , LEE, Haeng-Soon , RYU, Sun-Hwa
IPC分类号： C12N9/08
CPC分类号： C12N9/0065 , C12N15/8237 , C12N15/8238 , C12N15/8239
摘要： The present invention relates to a multiple stress inducible promoter, more particularly to a multiple stress inducible peroxidase promoter derived by Ipomoea batatas, a expression vector for production of transgenic plants with enhanced tolerance to multiple stress containing the promoter, a transgenic plant cell and plants transduced by the expression vector, and a method for generating the transgenic plants. Promoter of present invention will be biotechnologically useful for develpoment of industrial transgenic plants with enhanced toleransce to multiple stress and plant cell lines to produce useful proteins.
摘要翻译：本发明涉及多重胁迫诱导型启动子，更具体地涉及由番薯promoter promoter衍生的多重应激诱导型过氧化物酶启动子，用于产生具有增强的耐受多重胁迫的转基因植物的表达载体，其含有启动子，转基因植物细胞和植物转导通过表达载体，以及产生转基因植物的方法。本发明的启动子将在生物技术上用于具有增强的对多种应激和植物细胞系的耐受性以产生有用蛋白质的工业转基因植物的开发。

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