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    • 8. 发明申请
    • METHOD FOR RECOMBINATING PLASTID USING PROCARYOTIC RECOMBINASE GENE
    • 使用过程重组蛋白酶基因重组PLASTID的方法
    • WO2003060137A1
    • 2003-07-24
    • PCT/KR2002/002506
    • 2002-12-31
    • KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYLIU, Jang-RyolJEONG, Won-joongMIN, Sung-ranJEONG, Seok-wonHAN, Su-kyoung
    • LIU, Jang-RyolJEONG, Won-joongMIN, Sung-ranJEONG, Seok-wonHAN, Su-kyoung
    • C12N15/82
    • C12N15/8214C12N15/8213
    • The objective of this invention is to enhance the efficiency of plastid transformation using nuclear transformed plants in which the microbial recombinase A( recA ) is to target to (or expressed in) the plastid. This invention will be better explained by the following detailed descriptions. A plant is transformed with a nuclear transformation vector containing the microbial recA gene added with a plastid targeting sequence. In this nuclear transformed plant, the frequency of plastid transformation is enhanced greater than two-folds due to increased homologous recombination between the plastid transformation vector carrying genes of interest (or target genes) and the plastid genome. In addition, because plastid transformation is accomplished through a gradual process, adventitious shoots selected after being subjected to plastid transformation should be cut into explants, and then shoots regenerated from the explants are to be reselected until all of the plastids in the shoots are uniformly transformed. However, when the nuclear transformed plant is used, the number of reselection is reduced to 1/2 to 1/3 due to increased homologous recombination.
    • 本发明的目的是使用核转化植物提高质体转化的效率,其中微生物重组酶A(recA)靶向(或表达于)质体。 以下的详细说明将更好地说明本发明。 用含有质体靶向序列的微生物recA基因的核转化载体转化植物。 在这种核转化植物中,质体转化的频率由于携带感兴趣的基因(或靶基因)的质体转化载体和质体基因组之间的同源重组增加而增加了大于2倍。 另外,由于质体转化是通过逐步过程完成的,因此应将外来物质切割后进入质体转化后的不定芽进行切割,然后重新选择从外植体中再生的枝条,直到枝条中的所有质体均匀转化为止 。 然而,当使用核转化植物时,由于增加的同源重组,重选次数减少到1/2至1/3。