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1. 发明申请

WO2004064537A2 METHOD 审中-公开
标题翻译：方法
公开(公告)号：WO2004064537A2
公开(公告)日：2004-08-05
申请号：PCT/IB2004/000655
申请日：2004-01-15
申请人： DANISCO A/S , KREIJ, Arno, de , MADRID, Susan, Mampusta , MIKKELSEN, Jørn, Dalgaard , SØE, Jørn, Borch
发明人： KREIJ, Arno, de , MADRID, Susan, Mampusta , MIKKELSEN, Jørn, Dalgaard , SØE, Jørn, Borch
IPC分类号： A23L
CPC分类号： A21D8/042 , A23C19/0328 , A23D7/013 , A23G9/363 , A23L15/25 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6436 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918
摘要： A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.
摘要翻译：一种在食品中原位生产乳化剂的方法，其中将脂质酰基转移酶加入食品中。优选地，在不增加或不显着增加食品的游离脂肪酸含量的情况下生产乳化剂。优选地，脂质酰基转移酶是能够将酰基从脂质转移到以下酰基受体中的一种或多种：甾醇，甾烷醇，碳水化合物，蛋白质或其亚单位的甘油。优选地，除了乳化剂之外，可以制备一种或多种甾烷醇酯或甾烷醇酯或蛋白酯或碳水化合物酯或甘油二酯或甘油单酯。这些中的一种或多种可以用作另外的乳化剂。

2. 发明申请

WO2004064987A3 METHOD 审中-公开
标题翻译：方法
公开(公告)号：WO2004064987A3
公开(公告)日：2006-03-23
申请号：PCT/IB2004000575
申请日：2004-01-15
申请人： DANISCO , KREIJ ARNO DE , MADRID SUSAN MAMPUSTA , MIKKELSEN JOERN DALGAARD , SOEE JOERN BORCH
发明人： KREIJ ARNO DE , MADRID SUSAN MAMPUSTA , MIKKELSEN JOERN DALGAARD , SOEE JOERN BORCH
IPC分类号： A21D8/04 , A23C19/032 , A23D7/01 , A23G9/36 , A23L1/03 , A23L1/035 , A23L1/24 , A23L1/30 , A23L1/32 , C11C3/10 , C12N9/10 , C12N9/18 , C12P7/62 , C12P7/64 , C12Q1/48 , C12N15/54 , C12N11/00 , C12N15/55
CPC分类号： A21D8/042 , A23C19/0328 , A23D7/013 , A23G9/363 , A23L15/25 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6436 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918
摘要： A method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxy acid ester, which method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water, wherein said acyl donor is a lipid substrate selected from one or more of the group consisting of a phospholipid, a lysophospholipid, a triacylglyceride, a diglyceride, a glycolipid or a lysoglycolipid and said acyl acceptor is selected from one or more of the group consisting of a carbohydrate, a protein, a protein subunit, or a hydroxy acid; and contacting the admixture with a lipid acyltransferase, such that said lipid acyltransferase catalyses one or both of the following reactions; alcoholysis or transesterification.
摘要翻译：一种生产碳水化合物酯，蛋白质酯，蛋白质亚基酯或羟基酸酯中的一种或多种的方法，该方法包括将酰基供体，酰基受体和水混合以产生高水环境，其包含5-98％水，其中所述酰基供体是选自磷脂，溶血磷脂，三酰基甘油酯，甘油二酯，糖脂或溶血糖脂中的一种或多种的脂质底物，并且所述酰基受体选自以下组中的一种或多种：由碳水化合物，蛋白质，蛋白质亚基或羟基酸组成; 并使所述混合物与脂质酰基转移酶接触，使得所述脂质酰基转移酶催化以下反应中的一个或两个; 醇解或酯交换。

3. 发明申请

WO2004064537A3 METHOD FOR THE IN SITU PRODUCTION OF AN EMULSIFIER IN FOODSTUFF 审中-公开
标题翻译：在食品生产过程中的一种乳化剂的方法
公开(公告)号：WO2004064537A3
公开(公告)日：2006-03-23
申请号：PCT/IB2004000655
申请日：2004-01-15
申请人： DANISCO , KREIJ ARNO DE , MADRID SUSAN MAMPUSTA , MIKKELSEN JOERN DALGAARD , SOEE JOERN BORCH
发明人： KREIJ ARNO DE , MADRID SUSAN MAMPUSTA , MIKKELSEN JOERN DALGAARD , SOEE JOERN BORCH
IPC分类号： A21D8/04 , A23C19/032 , A23D7/01 , A23G9/36 , A23L1/03 , A23L1/035 , A23L1/24 , A23L1/30 , A23L1/32 , C11C3/10 , C12N9/10 , C12N9/18 , C12P7/62 , C12P7/64 , C12Q1/48 , C12N15/54 , C12N11/00 , C12N15/55 , C12Q1/44
CPC分类号： A21D8/042 , A23C19/0328 , A23D7/013 , A23G9/363 , A23L15/25 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6436 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918
摘要： A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.
摘要翻译：一种在食品中原位生产乳化剂的方法，其中将脂质酰基转移酶加入食品中。优选地，在不增加或不显着增加食品的游离脂肪酸含量的情况下生产乳化剂。优选地，脂质酰基转移酶是能够将酰基从脂质转移到以下酰基受体中的一种或多种：甾醇，甾烷醇，碳水化合物，蛋白质或其亚单位的甘油。优选地，除了乳化剂之外，可以制备一种或多种甾烷醇酯或甾烷醇酯或蛋白酯或碳水化合物酯或甘油二酯或甘油单酯。这些中的一种或多种可以用作另外的乳化剂。

4. 发明申请

WO2009024736A1 PROTEIN 审中-公开
标题翻译：蛋白
公开(公告)号：WO2009024736A1
公开(公告)日：2009-02-26
申请号：PCT/GB2008/000676
申请日：2008-02-27
申请人： DANISCO A/S , KREIJ, Arno, De , MADRID, Susan, Mampusti , MIKKELSEN, Jørn, Dalgaard , SOE, Jørn, Borch , TURNER, Mark , GOODWINS, Jonathan
发明人： KREIJ, Arno, De , MADRID, Susan, Mampusti , MIKKELSEN, Jørn, Dalgaard , SOE, Jørn, Borch , TURNER, Mark , GOODWINS, Jonathan
IPC分类号： C07K14/195 , C12P7/64 , C12N9/10 , A21D8/04 , A23C19/032 , C12Q1/48
CPC分类号： A21D8/042 , A23C19/0328 , A23C19/0684 , A23L15/25 , A23L29/06 , C12N9/1029 , C12N9/18 , C12N11/00 , C12Y301/01
摘要： A lipid acyltransferase is described. The lipid acyltransferase comprises the amino acid sequence shown as SEQ ID No. 90, or an amino acid sequence which has 95% or more identity with SEQ ID No. 90.
摘要翻译：描述脂质酰基转移酶。脂质酰基转移酶包含SEQ ID No.90所示的氨基酸序列或与SEQ ID No.90具有95％以上同一性的氨基酸序列。

5. 发明申请

WO2005066347A1 PROTEINS 审中-公开
标题翻译：蛋白质
公开(公告)号：WO2005066347A1
公开(公告)日：2005-07-21
申请号：PCT/IB2004/004378
申请日：2004-12-23
申请人： DANISCO A/S , KELLET-SMITH, Anja Hemmingsen , LORENTSEN, Rikke, Høegh , SØE, jørn, Borch , MIKKELSON, Jørn, Dalgaard , KREIJ, Arno de
发明人： KELLET-SMITH, Anja Hemmingsen , LORENTSEN, Rikke, Høegh , SØE, jørn, Borch , MIKKELSON, Jørn, Dalgaard , KREIJ, Arno de
IPC分类号： C12N15/54
CPC分类号： C12N9/1029 , C12Y203/01085
摘要： The present invention relates to a method of producing a variant glycolipid acyltransferase enzyme comprising: (a) selecting a parent enzyme which is a glycolipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S; (b) modifying one or more amino acids to produce a variant glycolipid acyltransferase; (c) testing the variant glycolipid acyltransferase for transferase activity, an optionally hydrolytic activity, on a galactolipid substrate, and optionally a phospholipid substrate and/or optionally a triglyceride substrate; (d) selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme; and optionally (e) preparing a quantity of the variant enzyme. The present invention further relates to variant lipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S, and wherein the variant enzyme comprises one or more amino acid modifications compared with a parent sequence at any one or more of the amino acid residues defined in set 2, set 4, set 6 or set 7.
摘要翻译：本发明涉及一种生产变体糖脂酰基转移酶的方法，包括：（a）选择亲糖基酶，其为糖脂酰基转移酶，其特征在于该酶包含氨基酸序列基序GDSX，其中X是一个或多个以下氨基酸残基L，A，V，I，F，Y，H，Q，T，N，M或S; （b）修饰一个或多个氨基酸以产生变体糖脂酰基转移酶; （c）测试变体糖脂酰基转移酶对半乳糖脂底物和任选的磷脂底物和/或任选的甘油三酯底物的转移酶活性，任选水解活性; （d）与母体酶相比，选择对半乳糖脂增强活性的变体酶; 和（e）制备一定量的变体酶。本发明还涉及变体脂质酰基转移酶，其特征在于该酶包含氨基酸序列基序GDSX，其中X是以下氨基酸残基中的一个或多个L，A，V，I，F，Y，H，Q ，T，N，M或S，并且其中所述变体酶与第2组，第4组，第6组或第7组中定义的任何一个或多个氨基酸残基与母体序列相比包含一个或多个氨基酸修饰。

6. 发明申请

WO2004064987A2 METHOD 审中-公开
标题翻译：方法
公开(公告)号：WO2004064987A2
公开(公告)日：2004-08-05
申请号：PCT/IB2004/000575
申请日：2004-01-15
申请人： DANISCO A/S , KREIJ, Arno De , MADRID, Susan, Mampusta , MIKKELSEN, Jørn, Dalgaard , SØE, Jørn, Borch
发明人： KREIJ, Arno De , MADRID, Susan, Mampusta , MIKKELSEN, Jørn, Dalgaard , SØE, Jørn, Borch
IPC分类号： B01F
CPC分类号： A21D8/042 , A23C19/0328 , A23D7/013 , A23G9/363 , A23L15/25 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6436 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918
摘要： A method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxy acid ester, which method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water, wherein said acyl donor is a lipid substrate selected from one or more of the group consisting of a phospholipid, a lysophospholipid, a triacylglyceride, a diglyceride, a glycolipid or a lysoglycolipid and said acyl acceptor is selected from one or more of the group consisting of a carbohydrate, a protein, a protein subunit, or a hydroxy acid; and contacting the admixture with a lipid acyltransferase, such that said lipid acyltransferase catalyses one or both of the following reactions; alcoholysis or transesterification.
摘要翻译：一种生产碳水化合物酯，蛋白质酯，蛋白质亚基酯或羟基酸酯中的一种或多种的方法，该方法包括将酰基供体，酰基受体和水混合以产生高水环境，其包含5-98％水，其中所述酰基供体是选自磷脂，溶血磷脂，三酰基甘油酯，甘油二酯，糖脂或溶血糖脂中的一种或多种的脂质底物，并且所述酰基受体选自以下组中的一种或多种：由碳水化合物，蛋白质，蛋白质亚基或羟基酸组成; 并使所述混合物与脂质酰基转移酶接触，使得所述脂质酰基转移酶催化以下反应中的一个或两个; 醇解或酯交换。

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