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1. 发明申请

WO2004094478A2 CLEAVAGE OF FUSION PROTEINS USING GRANZYME B PROTEASE 审中-公开
标题翻译：使用GRANZYME B蛋白的融合蛋白的清除
公开(公告)号：WO2004094478A2
公开(公告)日：2004-11-04
申请号：PCT/DK2004/000282
申请日：2004-04-23
申请人： BOREAN PHARMA A/S , LORENTSEN, Rikke, Høegh , FYNBO, Charlotte, Harkjær
发明人： LORENTSEN, Rikke, Høegh , FYNBO, Charlotte, Harkjær
IPC分类号： C07K19/00
CPC分类号： C12N9/6467 , C07K2319/50 , C12N15/62
摘要： A method for the preparation of a polypeptide of interest in authentic form by enzymatic cleavage of fusion proteins using Granzyme B protease (EC 3.4.21.79). There is also provided fusion proteins comprising a polypeptide of interest and a fusion partner, wherein the junction region between the polypeptide of interest and the fusion partner comprises a Granzyme B protease cleavage site adjacent to the polypeptide of interest, and a human Granzyme B protease variant wherein the Cystein residue no. 228 (chymotrypsinogen numbering) is mutated to Phenylalanine.
摘要翻译：通过使用Granzyme B蛋白酶的酶切割融合蛋白来制备感兴趣的多肽的方法（EC 3.4.21.79）。还提供了包含感兴趣多肽和融合配偶体的融合蛋白，其中感兴趣的多肽和融合配偶体之间的连接区域包含与感兴趣的多肽相邻的颗粒酶B蛋白酶切割位点，以及人粒酶B蛋白酶变体其中半胱氨酸残基号 228（胰凝乳蛋白酶原编号）突变为苯丙氨酸。

2. 发明申请

WO2008090395A1 PRODUCTION OF A LIPID ACYLTRANSFERASE FROM TRANSFORMED BACILLUS LICHENIFORMIS CELLS 审中-公开
标题翻译：从转化的BACILLUS LICHENIFORMIS细胞中产生脂质过氧化物酶
公开(公告)号：WO2008090395A1
公开(公告)日：2008-07-31
申请号：PCT/IB2007/000558
申请日：2007-01-25
申请人： DANISCO A/S , KOLKMAN, Marc , MIKKELSEN, Jørn, Dalgaard , LORENTSEN, Rikke, Høegh
发明人： KOLKMAN, Marc , MIKKELSEN, Jørn, Dalgaard , LORENTSEN, Rikke, Høegh
IPC分类号： C12N9/18 , C12N15/75 , C12N1/21
CPC分类号： C12N9/18 , C12N9/1029 , C12N15/75
摘要： The present invention relates to a method for the production of a lipid acyltransferase comprising the steps of: (i) providing a Bacillus licheniformis cell; (ii) transforming the Bacillus licheniformis cell with a heterologous nucleotide sequence encoding a lipid acyltransferase; and (iii) expressing the lipid acyltransferase in the cell under the control of a promoter sequence. In addition, the present invention further relates to the use of Bacillus licheniformis to express a lipid acyltransferase, a Bacillus licheniformis host cell comprising a heterologous lipid acyltransferase and a vector comprising a nucleotide sequence encoding a lipid acyltransferase operably linked to a promoter sequence homologous to B. licheniformis .
摘要翻译：本发明涉及一种生产脂质酰基转移酶的方法，包括以下步骤：（i）提供地衣芽孢杆菌细胞; （ii）用编码脂质酰基转移酶的异源核苷酸序列转化地衣芽孢杆菌细胞; 和（iii）在启动子序列控制下的细胞中表达脂质酰基转移酶。此外，本发明还涉及地衣芽孢杆菌表达脂质酰基转移酶，包含异源脂质酰基转移酶的地衣芽孢杆菌宿主细胞的使用和包含编码脂质酰基转移酶的核苷酸序列的载体，所述核苷酸序列可操作地连接到与B同源的启动子序列地衣芽孢杆菌

3. 发明申请

WO2005066347A1 PROTEINS 审中-公开
标题翻译：蛋白质
公开(公告)号：WO2005066347A1
公开(公告)日：2005-07-21
申请号：PCT/IB2004/004378
申请日：2004-12-23
申请人： DANISCO A/S , KELLET-SMITH, Anja Hemmingsen , LORENTSEN, Rikke, Høegh , SØE, jørn, Borch , MIKKELSON, Jørn, Dalgaard , KREIJ, Arno de
发明人： KELLET-SMITH, Anja Hemmingsen , LORENTSEN, Rikke, Høegh , SØE, jørn, Borch , MIKKELSON, Jørn, Dalgaard , KREIJ, Arno de
IPC分类号： C12N15/54
CPC分类号： C12N9/1029 , C12Y203/01085
摘要： The present invention relates to a method of producing a variant glycolipid acyltransferase enzyme comprising: (a) selecting a parent enzyme which is a glycolipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S; (b) modifying one or more amino acids to produce a variant glycolipid acyltransferase; (c) testing the variant glycolipid acyltransferase for transferase activity, an optionally hydrolytic activity, on a galactolipid substrate, and optionally a phospholipid substrate and/or optionally a triglyceride substrate; (d) selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme; and optionally (e) preparing a quantity of the variant enzyme. The present invention further relates to variant lipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S, and wherein the variant enzyme comprises one or more amino acid modifications compared with a parent sequence at any one or more of the amino acid residues defined in set 2, set 4, set 6 or set 7.
摘要翻译：本发明涉及一种生产变体糖脂酰基转移酶的方法，包括：（a）选择亲糖基酶，其为糖脂酰基转移酶，其特征在于该酶包含氨基酸序列基序GDSX，其中X是一个或多个以下氨基酸残基L，A，V，I，F，Y，H，Q，T，N，M或S; （b）修饰一个或多个氨基酸以产生变体糖脂酰基转移酶; （c）测试变体糖脂酰基转移酶对半乳糖脂底物和任选的磷脂底物和/或任选的甘油三酯底物的转移酶活性，任选水解活性; （d）与母体酶相比，选择对半乳糖脂增强活性的变体酶; 和（e）制备一定量的变体酶。本发明还涉及变体脂质酰基转移酶，其特征在于该酶包含氨基酸序列基序GDSX，其中X是以下氨基酸残基中的一个或多个L，A，V，I，F，Y，H，Q ，T，N，M或S，并且其中所述变体酶与第2组，第4组，第6组或第7组中定义的任何一个或多个氨基酸残基与母体序列相比包含一个或多个氨基酸修饰。

4. 发明申请

WO2010122532A3 FEED SUPPLEMENT 审中-公开
公开(公告)号：WO2010122532A3
公开(公告)日：2010-10-28
申请号：PCT/IB2010/051804
申请日：2010-04-23
申请人： DANISCO A/S , ISAKSEN, Mai Faurschou , LORENTSEN, Rikke Høegh , PLUMSTEAD, Peter , ROMERO MILLÁN, Luis Fernando , MADRID, Susan , CHERRY, Lin , WARD, Michael , ZARGAHI, Masoud Rajabi
发明人： ISAKSEN, Mai Faurschou , LORENTSEN, Rikke Høegh , PLUMSTEAD, Peter , ROMERO MILLÁN, Luis Fernando , MADRID, Susan , CHERRY, Lin , WARD, Michael , ZARGAHI, Masoud Rajabi
IPC分类号： A23K1/165 , C12N9/16 , C12N9/20
摘要： The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.5.

5. 发明申请

WO2010122531A2 METHOD 审中-公开
标题翻译：方法
公开(公告)号：WO2010122531A2
公开(公告)日：2010-10-28
申请号：PCT/IB2010/051802
申请日：2010-04-23
申请人： DANISCO A/S , MADRID, Susan M , LIN, Cherry , ZARGAHI, Masoud Rajabi , LORENTSEN, Rikke Høegh , ISAKSEN, Mai Faurschou , WARD, Michael
发明人： MADRID, Susan M , LIN, Cherry , ZARGAHI, Masoud Rajabi , LORENTSEN, Rikke Høegh , ISAKSEN, Mai Faurschou , WARD, Michael
IPC分类号： C12N9/20
CPC分类号： C12N9/20 , A23K20/189 , A23L29/06 , A23L33/14 , A23V2002/00 , C12N15/80 , C12Y301/01003
摘要： The present invention provides a method of producing a lipolytic enzyme comprising the steps of: (i) providing a Trichoderma reesei cell comprising a) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 or an amino acid sequence which has at least 40% sequence identity to SEQ ID NO: 1 or 2; and/or b) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4, and/or c) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which has at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4 or the complement of any thereof under stringent conditions; and (ii) culturing the cell under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme.
摘要翻译：本发明提供一种生产脂肪分解酶的方法，包括以下步骤：（i）提供里氏木霉细胞，其包含a）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1所示的氨基酸序列或 SEQ ID NO：2或与SEQ ID NO：1或2具有至少40％序列同一性的氨基酸序列; 和/或b）编码脂解酶的至少一种异源核苷酸序列，其中所述核苷酸序列包含SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列 SEQ ID NO：3或SEQ ID NO：4，和/或c）至少一个编码脂肪分解酶的异源核苷酸序列，其中所述核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或在严格条件下与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性或其任何互补序列的核苷酸序列; 和（ii）在允许表达编码所述脂肪分解酶的所述异源核苷酸序列的条件下培养细胞。

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