专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2010133644A3 AMYLASE POLYPEPTIDES 审中-公开
公开(公告)号：WO2010133644A3
公开(公告)日：2011-01-27
申请号：PCT/EP2010056913
申请日：2010-05-19
申请人： DANISCO , KRAGH KARSTEN MATTHIAS , KELLET-SMITH ANJA HEMMINGSEN , MIKKELSEN RENE , MEJLDAL RIE , JENNER RIKKE L BUNDGAARD
发明人： KRAGH KARSTEN MATTHIAS , KELLET-SMITH ANJA HEMMINGSEN , MIKKELSEN RENE , MEJLDAL RIE , JENNER RIKKE L BUNDGAARD
IPC分类号： C12N9/28 , A23L13/00
CPC分类号： C12P19/14 , C12N9/2417
摘要： This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products.
摘要翻译：本发明涉及多肽，更具体地涉及淀粉酶多肽和编码这些多肽的核酸及其用途。作为生产食品或饲料产品的非麦芽糖外消旋酶。

2. 发明申请

WO2009083592A1 PSEUDOMONAS SACCHAROPHILA G4-AMYLASE VARIANTS AND USES THEREOF 审中-公开
标题翻译： PSEUDOMONAS SACCHAROPHILA G4-氨基酸变体及其用途
公开(公告)号：WO2009083592A1
公开(公告)日：2009-07-09
申请号：PCT/EP2008/068375
申请日：2008-12-30
申请人： DANISCO A/S , Danisco US Inc., Genencor Division , KRAGH, Karsten, Matthias , KELLET-SMITH, Anja Hemmingsen , SHAW, Andrew , CHIN, Regina
发明人： KRAGH, Karsten, Matthias , KELLET-SMITH, Anja Hemmingsen , SHAW, Andrew , CHIN, Regina
IPC分类号： C12N9/28 , A21D8/04 , A23L1/09
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/35 , Y02E50/16 , Y02E50/17
摘要： Pseudomonas saccharophila G4-forming amylase (PS4) variants, and nucleic acids encoding these, and their uses in producing food products and in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch are provided.
摘要翻译：提供了糖酵母（Saccharophila）G4形成淀粉酶（PS4）变体，以及编码它们的核酸，以及它们在食品生产中的用途和在酶催化的高温液化步骤中从淀粉例如玉米淀粉生产乙醇的用途。

3. 发明申请

WO2009088465A3 A PROCESS OF OBTAINING ETHANOL WITHOUT GLUCOAMYLASE USING PSEUDOMONAS SACCHAROPHILA G4-AMYLASE AND VARIANTS THEREOF 审中-公开
公开(公告)号：WO2009088465A3
公开(公告)日：2009-07-16
申请号：PCT/US2008/014105
申请日：2008-12-30
申请人： DANISCO US INC., GENENCOR DIVISION , SHAW, Andrew , CHIN, Regina , KRAGH, Karsten Matthias , KELLET-SMITH, Anja Hemmingsen
发明人： SHAW, Andrew , CHIN, Regina , KRAGH, Karsten Matthias , KELLET-SMITH, Anja Hemmingsen
IPC分类号： C12N9/28 , A23L1/09
摘要： Pseudomonas saccharophila G4 forming amylase (PS4), and variants thereof, advantageously can be used in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch. PS4 produces significant amounts of maltotrioses, which can be utilized by S. cerevisiae in a subsequent fermentation step to produce ethanol. This property of PS4 advantageously allows ethanol to be produced from liquefacted starch in the absence of a saccharification step. PS4 variants are provided that exhibit improved properties, such as thermostability and/or altered exo-specific and endo-specific amylase activity.

4. 发明申请

WO2010133644A2 AMYLASE POLYPEPTIDES 审中-公开
公开(公告)号：WO2010133644A2
公开(公告)日：2010-11-25
申请号：PCT/EP2010/056913
申请日：2010-05-19
申请人： DANISCO A/S , KRAGH, Karsten Matthias , KELLET-SMITH, Anja, Hemmingsen , MIKKELSEN, René , MEJLDAL, Rie , JENNER, Rikke L. Bundgaard
发明人： KRAGH, Karsten Matthias , KELLET-SMITH, Anja, Hemmingsen , MIKKELSEN, René , MEJLDAL, Rie , JENNER, Rikke L. Bundgaard
IPC分类号： C12N9/28
CPC分类号： C12P19/14 , C12N9/2417
摘要： This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products.
摘要翻译：本发明涉及多肽，更具体地涉及淀粉酶多肽和编码这些多肽的核酸及其用途。作为生产食品或饲料产品的非麦芽糖外消旋酶。

5. 发明申请

WO2009088465A2 A PROCESS OF OBTAINING ETHANOL WITHOUT GLUCOAMYLASE USING PSEUDOMONAS SACCHAROPHILA G4-AMYLASE AND VARIANTS THEREOF 审中-公开
标题翻译：使用PSEUDOMONAS SACCHAROPHILA G4-氨基酸获得乙醇胺的方法及其变体
公开(公告)号：WO2009088465A2
公开(公告)日：2009-07-16
申请号：PCT/US2008014105
申请日：2008-12-30
申请人： DANISCO US INC GENENCOR DIV , SHAW ANDREW , CHIN REGINA , KRAGH KARSTEN MATTHIAS , KELLET-SMITH ANJA HEMMINGSEN
发明人： SHAW ANDREW , CHIN REGINA , KRAGH KARSTEN MATTHIAS , KELLET-SMITH ANJA HEMMINGSEN
IPC分类号： C12N9/28 , A23L1/09
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/35 , Y02E50/16 , Y02E50/17
摘要： Pseudomonas saccharophila G4 forming amylase (PS4), and variants thereof, advantageously can be used in an enzyme-catalyzed high temperature liquefaction step to produce ethanol from starch, e.g., cornstarch. PS4 produces significant amounts of maltotrioses, which can be utilized by S. cerevisiae in a subsequent fermentation step to produce ethanol. This property of PS4 advantageously allows ethanol to be produced from liquefacted starch in the absence of a saccharification step. PS4 variants are provided that exhibit improved properties, such as thermostability and/or altered exo-specific and endo-specific amylase activity.
摘要翻译：糖酵母糖酵母G4形成淀粉酶（PS4）及其变体有利地可用于酶催化的高温液化步骤以从淀粉例如玉米淀粉产生乙醇。 PS4产生显着量的麦芽三糖，其可以在随后的发酵步骤中被酿酒酵母用于产生乙醇。 PS4的这种性质有利地允许在没有糖化步骤的情况下从液化淀粉生产乙醇。提供了PS4变体，其具有改进的性质，例如热稳定性和/或改变的外切特异性和内切特异性淀粉酶活性。

6. 发明申请

WO2005066347A1 PROTEINS 审中-公开
标题翻译：蛋白质
公开(公告)号：WO2005066347A1
公开(公告)日：2005-07-21
申请号：PCT/IB2004/004378
申请日：2004-12-23
申请人： DANISCO A/S , KELLET-SMITH, Anja Hemmingsen , LORENTSEN, Rikke, Høegh , SØE, jørn, Borch , MIKKELSON, Jørn, Dalgaard , KREIJ, Arno de
发明人： KELLET-SMITH, Anja Hemmingsen , LORENTSEN, Rikke, Høegh , SØE, jørn, Borch , MIKKELSON, Jørn, Dalgaard , KREIJ, Arno de
IPC分类号： C12N15/54
CPC分类号： C12N9/1029 , C12Y203/01085
摘要： The present invention relates to a method of producing a variant glycolipid acyltransferase enzyme comprising: (a) selecting a parent enzyme which is a glycolipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S; (b) modifying one or more amino acids to produce a variant glycolipid acyltransferase; (c) testing the variant glycolipid acyltransferase for transferase activity, an optionally hydrolytic activity, on a galactolipid substrate, and optionally a phospholipid substrate and/or optionally a triglyceride substrate; (d) selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme; and optionally (e) preparing a quantity of the variant enzyme. The present invention further relates to variant lipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S, and wherein the variant enzyme comprises one or more amino acid modifications compared with a parent sequence at any one or more of the amino acid residues defined in set 2, set 4, set 6 or set 7.
摘要翻译：本发明涉及一种生产变体糖脂酰基转移酶的方法，包括：（a）选择亲糖基酶，其为糖脂酰基转移酶，其特征在于该酶包含氨基酸序列基序GDSX，其中X是一个或多个以下氨基酸残基L，A，V，I，F，Y，H，Q，T，N，M或S; （b）修饰一个或多个氨基酸以产生变体糖脂酰基转移酶; （c）测试变体糖脂酰基转移酶对半乳糖脂底物和任选的磷脂底物和/或任选的甘油三酯底物的转移酶活性，任选水解活性; （d）与母体酶相比，选择对半乳糖脂增强活性的变体酶; 和（e）制备一定量的变体酶。本发明还涉及变体脂质酰基转移酶，其特征在于该酶包含氨基酸序列基序GDSX，其中X是以下氨基酸残基中的一个或多个L，A，V，I，F，Y，H，Q ，T，N，M或S，并且其中所述变体酶与第2组，第4组，第6组或第7组中定义的任何一个或多个氨基酸残基与母体序列相比包含一个或多个氨基酸修饰。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式