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1. 发明申请

WO2005033340A3 METHODS AND KITS FOR HYBRIDIZING MULTIPLE PROBE PANELS TO NUCLEIC ACID SAMPLES 审中-公开
公开(公告)号：WO2005033340A3
公开(公告)日：2005-04-14
申请号：PCT/US2004/032446
申请日：2004-09-29
申请人： APPLERA CORPORATION , WILLIAMS, Brett , JOHANSEN, Jack , HYLDIG-NIELSEN, Jens, J.
发明人： WILLIAMS, Brett , JOHANSEN, Jack , HYLDIG-NIELSEN, Jens, J.
IPC分类号： C12Q1/68
摘要： Described herein are methods and kits that employ multiple probe sets in combination with sequential steps of hybridization analysis for multiplex analysis and/or detection of nucleic acids having one or more distinguishable target sequences.

2. 发明申请

WO2008137728A8 SYSTEM AND METHOD FOR PROTEOMICS 审中-公开
标题翻译：系统与方法
公开(公告)号：WO2008137728A8
公开(公告)日：2009-12-23
申请号：PCT/US2008062482
申请日：2008-05-02
申请人： PROTEIN FOREST INC , JOHANSEN JACK , GARLICK RUSSELL K , SKEA WILLIAM , HARALAMPU STEPHEN , KAGAN OREN
发明人： JOHANSEN JACK , GARLICK RUSSELL K , SKEA WILLIAM , HARALAMPU STEPHEN , KAGAN OREN
IPC分类号： G01N27/447
CPC分类号： G01N27/44795
摘要： Significantly higher yield and better resolution in pi gels are obtained by creating traps having two or more layers of gel (26, 28) containing closely stepped immobilized pH buffers. Proteins move from a pH at which they are negatively charged towards an anode at which they are positively charged. The gel layers (26, 28) containing immobilized pH buffers trap the proteins when the immobilized buffer pH and the protein pi are approximately the same. The protein is trapped within the second layer and not on the surface of or interface of the second layer.
摘要翻译：通过产生具有两层或更多层凝胶（26,28）的包含紧密阶梯的固定化pH缓冲液的阱，可以获得明显更高的产率和更好的分辨率。蛋白质从带负电荷的pH向负极移动。含有固定化pH值缓冲液的凝胶层（26,28）在固定化缓冲液pH和蛋白质pI大致相同时捕获蛋白质。蛋白质被捕获在第二层内，而不是在第二层的表面或界面上。

3. 发明申请

WO2003080834A2 PURIFICATION METHODS FOR OLIGONUCLEOTIDES AND THEIR ANALOGS 审中-公开
标题翻译：寡核苷酸及其类似物的纯化方法
公开(公告)号：WO2003080834A2
公开(公告)日：2003-10-02
申请号：PCT/GB2003/001161
申请日：2003-03-19
申请人： AVECIA BIOTECHNOLOGY INC. , AVECIA LIMITED , JOHANSEN, Jack, T.
发明人： JOHANSEN, Jack, T.
IPC分类号： C12N15/10
CPC分类号： C12N15/101
摘要： The present invention discloses methods for separating oligonucleotides from impurities. In the methods of the invention, a target oligonucleotide, in a mixture comprising the target oligonucleotide and an impurity, is separated from the impurity using a titratable anion exchange composition. The target oligonucleotide is bound to the titratable anion exchange composition and an eluting solution which increases in pH over time is passed through the titratable anion exchange composition with the target oligonucleotide bound thereon. Preferably, the eluting solution does not substantially increase its salt concentration. The target oligonucleotide is eluted and thereby separated from the impurity which either elutes at a lower pH or a higher pH than the target oligonucleotide.
摘要翻译：本发明公开了从杂质中分离寡核苷酸的方法。在本发明的方法中，使用可滴定阴离子交换组合物将包含目标寡核苷酸和杂质的混合物中的目标寡核苷酸与杂质分离。目标寡核苷酸与可滴定阴离子交换组合物结合，随着时间推移pH增加的洗脱溶液通过可滴定阴离子交换组合物，其上结合有目标寡核苷酸。优选地，洗脱溶液基本上不增加其盐浓度。目标寡核苷酸被洗脱，从而从杂质中分离出来，所述杂质在比目标寡核苷酸更低或更高的pH下洗脱。

4. 发明申请

WO2008137728A1 SYSTEM AND METHOD FOR PROTEOMICS 审中-公开
标题翻译：系统与方法
公开(公告)号：WO2008137728A1
公开(公告)日：2008-11-13
申请号：PCT/US2008/062482
申请日：2008-05-02
申请人： PROTEIN FOREST, INC. , GARLICK, Russell, K. , SKEA, William , HARALAMPU, Stephen , JOHANSEN, Jack , KAGAN, Oren
发明人： GARLICK, Russell, K. , SKEA, William , HARALAMPU, Stephen , JOHANSEN, Jack , KAGAN, Oren
IPC分类号： G01N27/447
CPC分类号： G01N27/44795
摘要： Significantly higher yield and better resolution in pi gels are obtained by creating traps having two or more layers of gel (26, 28) containing closely stepped immobilized pH buffers. Proteins move from a pH at which they are negatively charged towards an anode at which they are positively charged. The gel layers (26, 28) containing immobilized pH buffers trap the proteins when the immobilized buffer pH and the protein pi are approximately the same. The protein is trapped within the second layer and not on the surface of or interface of the second layer.
摘要翻译：通过产生具有两层或更多层凝胶（26,28）的包含紧密阶梯的固定化pH缓冲液的阱，可以获得明显更高的产率和更好的分辨率。蛋白质从带负电荷的pH向负极移动。含有固定化pH值缓冲液的凝胶层（26,28）在固定化缓冲液pH和蛋白质pI大致相同时捕获蛋白质。蛋白质被捕获在第二层内，而不是在第二层的表面或界面上。

5. 发明申请

WO2005033340A2 METHODS AND KITS FOR HYBRIDIZING MULTIPLE PROBE PANELS TO NUCLEIC ACID SAMPLES 审中-公开
标题翻译：用于将多个探针面板杂交到核酸样品的方法和工具
公开(公告)号：WO2005033340A2
公开(公告)日：2005-04-14
申请号：PCT/US2004032446
申请日：2004-09-29
申请人： APPLERA CORP , WILLIAMS BRETT , JOHANSEN JACK , HYLDIG-NIELSEN JENS J
发明人： WILLIAMS BRETT , JOHANSEN JACK , HYLDIG-NIELSEN JENS J
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6816 , C12Q1/6883 , C12Q2537/161 , C12Q2537/143 , C12Q2537/1373 , C12Q2565/518
摘要： Described herein are methods and kits that employ multiple probe sets in combination with sequential steps of hybridization analysis for multiplex analysis and/or detection of nucleic acids having one or more distinguishable target sequences.
摘要翻译：本文描述的是使用多个探针组合与杂交分析的顺序步骤进行多重分析和/或检测具有一个或多个可区分靶序列的核酸的方法和试剂盒。

6. 发明申请

WO2003080834A3 PURIFICATION METHODS FOR OLIGONUCLEOTIDES AND THEIR ANALOGS 审中-公开
公开(公告)号：WO2003080834A3
公开(公告)日：2003-10-02
申请号：PCT/GB2003/001161
申请日：2003-03-19
申请人： AVECIA BIOTECHNOLOGY INC. , AVECIA LIMITED , JOHANSEN, Jack, T.
发明人： JOHANSEN, Jack, T.
IPC分类号： C12N15/10
摘要： The present invention discloses methods for separating oligonucleotides from impurities. In the methods of the invention, a target oligonucleotide, in a mixture comprising the target oligonucleotide and an impurity, is separated from the impurity using a titratable anion exchange composition. The target oligonucleotide is bound to the titratable anion exchange composition and an eluting solution which increases in pH over time is passed through the titratable anion exchange composition with the target oligonucleotide bound thereon. Preferably, the eluting solution does not substantially increase its salt concentration. The target oligonucleotide is eluted and thereby separated from the impurity which either elutes at a lower pH or a higher pH than the target oligonucleotide.

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