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1. 发明申请

WO2008137728A8 SYSTEM AND METHOD FOR PROTEOMICS 审中-公开
标题翻译：系统与方法
公开(公告)号：WO2008137728A8
公开(公告)日：2009-12-23
申请号：PCT/US2008062482
申请日：2008-05-02
申请人： PROTEIN FOREST INC , JOHANSEN JACK , GARLICK RUSSELL K , SKEA WILLIAM , HARALAMPU STEPHEN , KAGAN OREN
发明人： JOHANSEN JACK , GARLICK RUSSELL K , SKEA WILLIAM , HARALAMPU STEPHEN , KAGAN OREN
IPC分类号： G01N27/447
CPC分类号： G01N27/44795
摘要： Significantly higher yield and better resolution in pi gels are obtained by creating traps having two or more layers of gel (26, 28) containing closely stepped immobilized pH buffers. Proteins move from a pH at which they are negatively charged towards an anode at which they are positively charged. The gel layers (26, 28) containing immobilized pH buffers trap the proteins when the immobilized buffer pH and the protein pi are approximately the same. The protein is trapped within the second layer and not on the surface of or interface of the second layer.
摘要翻译：通过产生具有两层或更多层凝胶（26,28）的包含紧密阶梯的固定化pH缓冲液的阱，可以获得明显更高的产率和更好的分辨率。蛋白质从带负电荷的pH向负极移动。含有固定化pH值缓冲液的凝胶层（26,28）在固定化缓冲液pH和蛋白质pI大致相同时捕获蛋白质。蛋白质被捕获在第二层内，而不是在第二层的表面或界面上。

2. 发明申请

WO2007106370A9 TRANSFER DEVICE FOR TWO - DIMENSIONAL ELECTROPHORESIS 审中-公开
标题翻译：用于二维电泳的转移装置
公开(公告)号：WO2007106370A9
公开(公告)日：2007-12-13
申请号：PCT/US2007005966
申请日：2007-03-08
申请人： PROTEIN FOREST INC , HARALAMPU STEPHEN , SKEA WILLIAM M , CHLENOV MARK , THOMPSON EDWARD
发明人： HARALAMPU STEPHEN , SKEA WILLIAM M , CHLENOV MARK , THOMPSON EDWARD
IPC分类号： G01N27/447 , C07K1/26
CPC分类号： C07K1/26 , C07K1/285 , G01N27/44773
摘要： Apparatus and method for transfer of analytes from a first dimension isoelectric focusing gel to a second dimension, such as an electrophoretic analytical gel. In a preferred embodiment, the device (10) facilitates the transfer of protein analytes from a digital proteome chip (12) to a second dimension electrophoretic analytical gel (18). The main features of the preferred embodiment are a recessed location (14) on a collar (16a, 16b), a mechanical clamp device (22) and stops (20a, 20b) that locate the first dimension gel at a fixed and known position in space, and a low density medium that assures uniform electrical contact between the first and second dimension analyses.The advantage of an apparatus that places the first dimension in a known position is that the final data analysis is greatly facilitated by precisely knowing the locations of the first dimension pH features. The medium that assures electrical contact also has the advantage of simplifying the final data analysis by assuring a uniform frame of reference on how the second dimension analysis was run.
摘要翻译：将分析物从第一维等电聚焦凝胶转移到第二维的装置和方法，例如电泳分析凝胶。在优选的实施方案中，装置（10）有助于将蛋白质分析物从数字蛋白质组芯片（12）转移到第二维电泳分析凝胶（18）。优选实施例的主要特征是在轴环（16a，16b）上的凹进位置（14），机械夹紧装置（22）和止动件（20a，20b），其将第一尺寸凝胶定位在固定的已知位置空间和低密度介质，其确保第一和第二维度分析之间的均匀电接触。将第一维度置于已知位置的装置的优点是通过精确地知道第一维度的位置来极大地促进最终数据分析第一维pH特征。确保电接触的介质还具有通过确保对第二维分析如何运行的统一的参考框架来简化最终数据分析的优点。

3. 发明申请

WO2007106370A2 TWO-DIMENSIONAL TRANSFER DEVICE 审中-公开
标题翻译：二维传输设备
公开(公告)号：WO2007106370A2
公开(公告)日：2007-09-20
申请号：PCT/US2007005966
申请日：2007-03-08
申请人： PROTEIN FOREST INC , HARALAMPU STEPHEN , SKEA WILLIAM M , CHLENOV MARK , THOMPSON EDWARD
发明人： HARALAMPU STEPHEN , SKEA WILLIAM M , CHLENOV MARK , THOMPSON EDWARD
IPC分类号： G01N27/447 , C07K1/26
CPC分类号： C07K1/26 , C07K1/285 , G01N27/44773
摘要： A system including apparatus and method of use that insures uniform contact and transfer of analytes from a first dimensional electrophoretic analytical gel to a second dimension, such as an electrophoretic analytical gel, has been developed. The device provides a means for transfer of analytes from a first dimension isoelectric focusing gel to a second dimension electrophoretic analytical gel. In a preferred embodiment, the device facilitates the transfer of protein analytes from a digital proteome chip to a second dimension electrophoretic analytical gel. The second dimension electrophoretic analytical gel can be for use with any technique known in the art, such as, but not limited to SDS-PAGE molecular weight analysis, secondary isoelectric focusing, and capillary electrophoresis. The main features of the preferred embodiment are a mechanical device that locates the first dimension analysis at a fixed and known position in space, and a low density medium that assures uniform electrical contact between the first and second dimension analyses. The advantage of an apparatus that places the first dimension in a known position is that the final data analysis is greatly facilitated by precisely knowing the locations of the first dimension pH features. The medium that assures electrical contact also has the advantage of simplifying the final data analysis by assuring a uniform frame of reference on how the second dimension analysis was run.
摘要翻译：已经开发了包括使分析物从第一维电泳分析凝胶到第二维度（例如电泳分析凝胶）的均匀接触和转移的装置和方法的系统。该装置提供了将分析物从第一维等电聚焦凝胶转移到第二尺寸电泳分析凝胶的方法。在优选的实施方案中，该装置有助于将蛋白质分析物从数字蛋白质组芯片转移到第二维电泳分析凝胶。第二维电泳分析凝胶可用于本领域已知的任何技术，例如但不限于SDS-PAGE分子量分析，二次等电聚焦和毛细管电泳。优选实施例的主要特征是将第一维度分析定位在空间中的固定和已知位置的机械装置以及确保第一和第二尺寸分析之间的均匀电接触的低密度介质。将第一维度置于已知位置的装置的优点是通过精确地知道第一维度pH特征的位置来极大地促进最终数据分析。确保电接触的介质还具有通过确保对第二维分析如何运行的统一的参考框架来简化最终数据分析的优点。

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