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1. 发明申请

WO2012046061A3 CLOSTRIDIUM DIFFICILE ANTIGENS 审中-公开
标题翻译：细菌抗生素
公开(公告)号：WO2012046061A3
公开(公告)日：2012-12-20
申请号：PCT/GB2011051910
申请日：2011-10-05
申请人： HEALTH PROT AGENCY , MICROPHARM LTD , SHONE CLIFFORD , ROBERTS APRIL , AHERN HELEN , MAYNARD-SMITH MICHAEL , LANDON JOHN
发明人： SHONE CLIFFORD , ROBERTS APRIL , AHERN HELEN , MAYNARD-SMITH MICHAEL , LANDON JOHN
IPC分类号： C07K14/33 , A61K39/08 , C07K16/12
CPC分类号： A61K39/08 , A61K38/164 , A61K39/40 , A61K2039/505 , C07K14/33 , C07K16/1282 , C07K2317/76 , C07K2319/00
摘要： The present invention relates to recombinant Clostridium difficile antigens based on a fusion protein that consists of or comprises a first amino acid sequence and a second amino acid sequence, wherein: a) the first amino acid sequence is provided by an amino acid sequence that has at least 80% sequence identity with an amino acid sequence consisting of residues 500-1850 of a C. difficile Toxin A sequence or residues 1500-1851 of a C. difficile Toxin B sequence; and b) the second amino acid sequence is provided by an amino acid sequence that has at least 80% sequence identity with an amino acid sequence consisting of a long repeat unit located within amino acid residues 1851-2710 of a C. difficile Toxin A sequence or within amino acid residues 1852-2366 of a C. difficile Toxin B sequence; though with the proviso that the fusion protein is not a polypeptide comprising amino acid residues 543-2710 of a C. difficile Toxin A and with the proviso that the fusion protein is not a polypeptide comprising amino acid residues 543-2366 of a C. difficile Toxin B. Also provided is the use of said antigens for the prevention/ treatment/ suppression of Clostridium difficile infection (CDI), together with methods for generating said antigens, methods for generating antibodies that bind to said antigens, and the use of said antibodies for the prevention/ treatment/ suppression of CDI.
摘要翻译：本发明涉及基于融合蛋白的重组艰难梭菌抗原，所述融合蛋白由第一氨基酸序列和第二氨基酸序列组成，或者包含第一氨基酸序列和第二氨基酸序列，其中：a）第一氨基酸序列由氨基酸序列提供，与由艰难梭菌毒素A序列的残基500-1850或艰难梭菌毒素B序列的残基1500-1851组成的氨基酸序列具有至少80％的序列同一性; 和b）第二氨基酸序列由与位于艰难梭菌毒素A序列的氨基酸残基1851-2710内的长重复单元组成的氨基酸序列具有至少80％序列同一性的氨基酸序列提供或在艰难梭菌毒素B序列的氨基酸残基1852-2366内; 尽管附带条件是融合蛋白不是包含艰难梭菌毒素A的氨基酸残基543-2710的多肽，并且附带条件是该融合蛋白不是包含艰难梭菌的氨基酸残基543-2366的多肽毒素B.还提供了所述抗原用于预防/治疗/抑制艰难梭菌感染（CDI）的用途，以及用于产生所述抗原的方法，用于产生结合所述抗原的抗体的方法以及所述抗体用于预防/治疗/抑制CDI。

2. 发明申请

WO2006059093A3 FUSION PROTEINS 审中-公开
标题翻译：融合蛋白
公开(公告)号：WO2006059093A3
公开(公告)日：2006-08-17
申请号：PCT/GB2005004585
申请日：2005-12-01
申请人： HEALTH PROT AGENCY , ALLERGAN INC , FOSTER KEITH , CHADDOCK JOHN , MARKS PHILIP , STANCOMBE PATRICK , AOKI K ROGER , FRANCIS JOSEPH , STEWARD LANCE
发明人： FOSTER KEITH , CHADDOCK JOHN , MARKS PHILIP , STANCOMBE PATRICK , AOKI K ROGER , FRANCIS JOSEPH , STEWARD LANCE
IPC分类号： A61K38/16 , C07K14/00 , C07K14/435 , C07K19/00 , C12N15/62
CPC分类号： C12N15/62 , A61K38/00 , C07K14/575 , C07K2319/00
摘要： A single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of a nociceptive sensory afferent; a Targeting Moiety that is capable of binding to a Binding Site on the nociceptive sensory afferent, which Binding Site is capable of undergoing endocytosis to be incorporated into an endosome within the nociceptive sensory afferent; a protease cleavage site at which site the fusion protein is cleavable by a protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the Targeting Moiety; and a translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the nociceptive sensory afferent. Nucleic acid sequences encoding the polypeptide fusion proteins, methods of preparing same and uses thereof are also described.
摘要翻译：一种单链多肽融合蛋白，其包含：非细胞毒性蛋白酶或其片段，所述蛋白酶或蛋白酶片段能够切割伤害性感觉传入的胞外融合装置的蛋白质; 能够结合伤害性感觉传入的结合位点的靶向部位，该结合位点能够经历内吞作用以掺入伤害性感觉传入内的内体; 蛋白酶切割位点，其中融合蛋白可被蛋白酶切割，其中蛋白酶切割位点位于非细胞毒性蛋白酶或其片段与靶向部位之间; 以及易位区域，其能够将位于内体内的蛋白酶或蛋白酶片段穿过内体膜并转移到伤害性感觉传入物的胞质溶胶中。还描述了编码多肽融合蛋白的核酸序列，其制备方法及其用途。

3. 发明申请

WO2004024909A3 RECOMBINANT COLSTRIDIUM NEUROTOXIN FRAGMENTS 审中-公开
标题翻译：重组菌神经毒素片段
公开(公告)号：WO2004024909A3
公开(公告)日：2004-07-08
申请号：PCT/GB0303824
申请日：2003-09-12
申请人： HEALTH PROT AGENCY , SHONE CLIFFORD CHARLES , FOSTER KEITH ALAN , CHADDOCK JOHN , MARKS PHILIP , SUTTON MARK J , STANCOMBE PATRICK , WAYNE JONATHAN
发明人： SHONE CLIFFORD CHARLES , FOSTER KEITH ALAN , CHADDOCK JOHN , MARKS PHILIP , SUTTON MARK J , STANCOMBE PATRICK , WAYNE JONATHAN
IPC分类号： C12N15/09 , A61K20060101 , A61K38/00 , A61K38/16 , A61K39/00 , A61K39/08 , A61K47/48 , A61K48/00 , A61P31/04 , C07K14/33 , C07K14/65 , C07K19/00 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/52 , C12N15/31 , C12N15/62 , C12P21/02 , C12P21/04 , C12R1/145 , C12R1/19
CPC分类号： A61K39/08 , A61K38/00 , A61K39/00 , A61K47/6415 , A61K47/646 , A61K2039/505 , A61K2039/53 , A61K2039/627 , C07K14/33 , C07K2319/00 , C07K2319/20 , C07K2319/23 , C07K2319/50 , C07K2319/55 , C07K2319/705 , C07K2319/75 , C12N9/52 , C12N15/62 , Y10S530/825
摘要： A single polypeptide is provided which comprises first and second domains. The first domain enables the polypeptide to cleave one or more vesicle or plasma-membrane associated proteins essential to exocytosis, and the second domain enables the polypeptide to be translocated into a target cell or increases the solubility of the polypeptide, or both. The polypeptide thus combines useful properties of a clostridial toxin, such as a botulinum or tetanus toxin, without the toxicity associated with the natural molecule. The polypeptide can also contain a third domain that targets it to a specific cell, rendering the polypeptide useful in inhibition of exocytosis in target cells. Fusion proteins comprising the polypeptide, nucleic acids encoding the polypeptide and methods of making the polypeptide are also provided. Controlled activation of the polypeptide, is possible and the polypeptide can be incorporated into vaccines and toxin assays.
摘要翻译：提供了包含第一和第二结构域的单个多肽。第一结构域使多肽能够切割一种或多种与胞吐作用相关的囊泡或质膜相关的蛋白质，而第二结构域使多肽易位于靶细胞或增加多肽或两者的溶解度。因此，该多肽结合梭菌毒素如肉毒杆菌或破伤风毒素的有用性质，而不与天然分子相关的毒性。多肽还可以含有将其靶向特定细胞的第三结构域，使得多肽可用于抑制靶细胞中的胞吐作用。还提供了包含多肽的融合蛋白，编码多肽的核酸和制备多肽的方法。多肽的受控活化是可能的，并且多肽可以并入疫苗和毒素测定中。

4. 发明申请

WO2009063078A3 RADIATION DETECTION 审中-公开
标题翻译：辐射检测
公开(公告)号：WO2009063078A3
公开(公告)日：2009-07-09
申请号：PCT/EP2008065612
申请日：2008-11-14
申请人： HEALTH PROT AGENCY , TANNER RICK , EAKINS JONATHAN
发明人： TANNER RICK , EAKINS JONATHAN
IPC分类号： G01T3/00
CPC分类号： G01T3/00
摘要： An instrument for detecting radiation is provided, which comprises an inner core housing a neutron detector, and an outer core comprising a neutron-moderating material, the instrument further including at least one elongate thermal neutron guide located within the outer core and having an inner end that terminates proximal to the neutron detector. In use, the elongate thermal neutron guide channels thermal neutrons towards the neutron detector. Also provided is a method for using said instrument.
摘要翻译：提供了一种用于检测辐射的仪器，其包括容纳中子检测器的内芯和包含中子减速材料的外芯，所述仪器还包括位于外芯内的至少一个细长热中子引导件，并具有内端其终止于中子探测器的近端。在使用中，细长热中子引导通道朝向中子探测器的热中子。还提供了使用所述仪器的方法。

5. 发明申请

WO2006059113A3 FUSION PROTEINS COMPRISING A NON-CYTOTOXIC PROTEASE, A TARGETING MOIETY, A PROTEASE CLEAVAGE SITE AND A TRANSLOCATION DOMAIN 审中-公开
标题翻译：包含非细胞毒素蛋白酶，靶向性蛋白酶，蛋白酶切位点和转录结构域的融合蛋白
公开(公告)号：WO2006059113A3
公开(公告)日：2006-09-28
申请号：PCT/GB2005004606
申请日：2005-12-01
申请人： HEALTH PROT AGENCY , FOSTER KEITH , CHADDOCK JOHN , MARKS PHILIP , STANCOMBE PATRICK , DUROSE LYNDSEY
发明人： FOSTER KEITH , CHADDOCK JOHN , MARKS PHILIP , STANCOMBE PATRICK , DUROSE LYNDSEY
IPC分类号： C07K14/33 , A61K38/00 , C12N15/62
CPC分类号： C12N9/50 , A61K38/00 , C07K14/33 , C07K2319/06 , C07K2319/50 , C12N15/62
摘要： The invention provides a single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of a target cell; a Targeting Moiety that is capable of binding to a Binding Site on the target cell, which Binding Site is capable of undergoing endocytosis to be incorporated into an endosome within the target cell; a protease cleavage site at which site the fusion protein is cleavable by a protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the Targeting Moiety; and a translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the target cell.
摘要翻译：本发明提供了一种单链多肽融合蛋白，其包含：非细胞毒性蛋白酶或其片段，所述蛋白酶或蛋白酶片段能够切割靶细胞的胞外融合装置的蛋白质; 能够结合到目标细胞上的结合位点的靶向物质，该结合位点能够进行内吞作用以掺入靶细胞内的内体; 蛋白酶切割位点，其中融合蛋白可被蛋白酶切割，其中蛋白酶切割位点位于非细胞毒性蛋白酶或其片段与靶向部位之间; 以及易位区，其能够将位于内体内的蛋白酶或蛋白酶片段穿过内体膜并转移到靶细胞的胞质溶胶中。

6. 发明申请

WO2005035733A3 MODIFIED WHOLE CELL, CELL EXTRACT AND OMV-BASED VACCINES 审中-公开
标题翻译：改良的全细胞，细胞提取物和基于OMV的疫苗
公开(公告)号：WO2005035733A3
公开(公告)日：2005-12-29
申请号：PCT/GB2004004274
申请日：2004-10-08
申请人： HEALTH PROT AGENCY , GORRINGE ANDREW R , REDDIN KAREN M , GRAY-OWEN SCOTT D , BOULTON IAN C
发明人： GORRINGE ANDREW R , REDDIN KAREN M , GRAY-OWEN SCOTT D , BOULTON IAN C
IPC分类号： A61K9/00 , A61K9/127 , A61K35/74 , A61K38/00 , A61K39/095
CPC分类号： A61K39/095 , A61K38/00
摘要： Live vaccines, live-attenuated vaccines, dead vaccines, protein preparations for use in vaccines and OMVs for use in vaccines are free of CEACAM1-binding Opa, though max contain Opa variants that are antigenic but don't bind to CEACAM1.
摘要翻译：活疫苗，活减毒疫苗，死疫苗，疫苗中使用的蛋白质制剂以及疫苗中使用的OMV均不含CEACAM1结合Opa，但最大含有Opa变体，它们是抗原性的但不结合CEACAM1。

7. 发明申请

WO2004062677A9 BACTERIOPHAGE FOR THE TREATMENT OF BACTERIAL BIOFILMS 审中-公开
标题翻译：用于处理细菌生物膜的细菌
公开(公告)号：WO2004062677A9
公开(公告)日：2004-10-07
申请号：PCT/GB2004000073
申请日：2004-01-12
申请人： HEALTH PROT AGENCY , SHARP RICHARD , HUGHES GAVIN , HART ANTHONY , WALKER JAMES TAGGART
发明人： SHARP RICHARD , HUGHES GAVIN , HART ANTHONY , WALKER JAMES TAGGART
IPC分类号： A61K35/76 , A61K38/51 , A61P31/04
CPC分类号： A61K35/76 , A61K38/51 , C12N7/00 , C12N9/88 , C12N2795/00032 , C12N2795/10032 , C12N2795/10043 , C12Y402/02003 , A61K2300/00
摘要： The present invention provides a composition for treating a bacterial biofilm, comprising a first bacteriophage that is capable of infecting a bacterium within said biofilm, and a first polysaccharide lyase enzyme that is capable of degrading a polysaccharide within said biofilm. The composition preferably further comprises a pharmaceutically-acceptable antimicrobial agent, and may also include a DNase. Also provided are modified bacteriophages, methods of creating modified bacteriophages, use of said compositions and bacteriophage for treating biofilms, and methods for treating biofilms using the bacteriophages and compositions of the invention.
摘要翻译：本发明提供了一种用于治疗细菌生物膜的组合物，其包含能够感染所述生物膜内的细菌的第一噬菌体和能够降解所述生物膜内的多糖的第一多糖裂解酶。组合物优选还包含药学上可接受的抗微生物剂，并且还可以包括DNA酶。还提供了修饰的噬菌体，产生改性噬菌体的方法，所述组合物的用途和噬菌体用于治疗生物膜，以及使用本发明的噬菌体和组合物处理生物膜的方法。

8. 发明申请

WO03004520A3 MYCOBACTERIAL ANTIGENS EXPRESSED DURING LATENCY 审中-公开
标题翻译：延迟期间表达的MYCOBACTERIAL抗原
公开(公告)号：WO03004520A3
公开(公告)日：2003-10-16
申请号：PCT/GB0203052
申请日：2002-07-04
申请人： HEALTH PROT AGENCY , JAMES BRIAN WILLIAM , MARSH PHILIP , HAMPSHIRE TOBIAS
发明人： JAMES BRIAN WILLIAM , MARSH PHILIP , HAMPSHIRE TOBIAS
IPC分类号： G01N33/53 , A61K35/76 , A61K38/00 , A61K38/55 , A61K39/00 , A61K39/04 , A61K39/39 , A61K39/395 , A61K45/00 , A61K48/00 , A61P31/04 , C07K14/35 , C07K16/40 , C12N1/21 , C12N9/00 , C12N9/99 , C12N15/09 , C12N15/31 , C12Q1/06 , C12Q1/68 , C12R1/32 , G01N33/58 , G01N37/00 , A61K38/16 , C07K16/12 , C12N5/10 , C12N15/11 , C12N15/74 , C12N15/86 , G01N33/50
CPC分类号： C07K14/35 , A61K38/00 , A61K39/00
摘要： A method is provided for identifying mycobacterial genes that are induced or up-regulated under culture conditions that are nutrient-starving and which maintain mycobacterial latency, said conditions being obtainable by batch fermentation of a mycobacterium for at least 20 days post-inoculation, when compared with culture conditions that are not nutrient-starving and which support exponential growth of said mycobacterium. Said induced or up-regulated genes form the basis of nucleic acid vaccines, or provide targets to allow preparation of attenuated mycobacteria for vaccines against mycobacterial infections. Similarly, peptides encoded by said induced or up-regulated genes are employed in vaccines. In a further embodiment, the identified genes/peptides provide the means for identifying the presence of a mycobacterial infection in a clinical sample by nucleic acid probe or antibody detection.
摘要翻译：提供了一种用于鉴定在营养饥饿的培养条件下诱导或上调的并且维持分枝杆菌潜伏期的分枝杆菌基因的方法，所述条件可通过分批杆菌分批发酵至少20天接种后获得，当比较其中培养条件不是营养饥饿并且支持所述分枝杆菌的指数生长。所述诱导的或上调的基因构成核酸疫苗的基础，或提供靶标，以允许制备减毒分枝杆菌用于针对分枝杆菌感染的疫苗。类似地，由所述诱导或上调基因编码的肽用于疫苗。在另一个实施方案中，所鉴定的基因/肽提供了通过核酸探针或抗体检测鉴定临床样品中分枝杆菌感染的存在的方法。

9. 发明申请

WO2010004336A3 STIMULATED CELL STANDARDS 审中-公开
标题翻译：刺激的细胞标准
公开(公告)号：WO2010004336A3
公开(公告)日：2010-04-29
申请号：PCT/GB2009050815
申请日：2009-07-09
申请人： HEALTH PROT AGENCY , STEBBINGS RICHARD JOHN
发明人： STEBBINGS RICHARD JOHN
IPC分类号： G01N33/96
CPC分类号： G01N33/96
摘要： Methods for producing stimulated, positive and negative control reference standard for monitoring intracellular cytokine levels and cytokine release in test samples by stimulating cells to produce cytokines in the presence of a cytokine release inhibitor, fixing the stimulated cells with a fixative such as paraformaldehyde, washing to remove excess fixatives and freeze-drying the stimulated, fixed cells. Methods for producing labelled reference standards for cell proliferation assays are also disclosed, in which proliferation-competent mammalian cells, isolated from a human or animal body are labelled with a label, such as a dye, that is divided between daughter cells during cell proliferation (e.g. carboxyfluorescein succinimidyl ester), the cells are stimulated to proliferate, the proliferated cells are fixed by addition of a fixative and then preserved by freeze drying or cryopreservation.
摘要翻译：通过在细胞因子释放抑制剂存在下刺激细胞产生细胞因子，用固定剂如多聚甲醛固定刺激的细胞，洗涤以测定受试样品中细胞内细胞因子水平和细胞因子释放，产生受刺激的阳性和阴性对照参照标准的方法去除多余的固定剂并冷冻干燥受刺激的固定细胞。还公开了产生用于细胞增殖测定的标记参考标准物的方法，其中从人体或动物体分离的增殖活性哺乳动物细胞用在细胞增殖过程中在子细胞之间分开的标记物（例如染料）标记例如羧基荧光素琥珀酰亚胺酯），刺激细胞增殖，通过加入固定剂固定增殖的细胞，然后通过冷冻干燥或冷冻保存来保存。

10. 发明申请

WO2008096177A3 DETECTION OF HUMAN PAPILLOMAVIRUS 审中-公开
标题翻译：人乳头瘤病毒的检测
公开(公告)号：WO2008096177A3
公开(公告)日：2008-10-16
申请号：PCT/GB2008050080
申请日：2008-02-08
申请人： HEALTH PROT AGENCY , CORLESS CAROLINE , GUIVER MALCOLM
发明人： CORLESS CAROLINE , GUIVER MALCOLM
IPC分类号： C12Q1/70
CPC分类号： C12Q1/708
摘要： There is provided an in vitro method of detecting human papillomavirus nucleic acid in a sample, comprising: (a) contacting said sample with forward and reverse oligonucleotide primers, wherein said primers bind to target sites in the human papillomavirus L1 gene, or the complement thereof, under conditions suitable to promote amplification of a portion of said human papillomavirus L1 gene or complement, thereby generating an amplicon; (b) contacting said amplicon with a probe, wherein the probe binds to a target site within said amplicon; and (c) detecting binding of said probe to said amplicon; wherein said forward primer binds to a target site having the sequence SEQ ID NO: 1; and wherein said reverse primer binds to a target site having the sequence SEQ ID NO: 2.
摘要翻译：提供了检测样品中人乳头瘤病毒核酸的体外方法，包括：（a）使所述样品与正向和反向寡核苷酸引物接触，其中所述引物与人乳头瘤病毒L1基因或其补体中的靶位点结合在适合于促进所述人乳头瘤病毒L1基因或补体的一部分扩增的条件下，由此产生扩增子; （b）使所述扩增子与探针接触，其中所述探针结合所述扩增子内的靶位点; 和（c）检测所述探针与所述扩增子的结合; 其中所述正向引物结合具有序列SEQ ID NO：1的靶位点; 并且其中所述反向引物与具有序列SEQ ID NO：2的靶位点结合。

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