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    • 4. 发明申请
    • MULTIPLY-SUBSTITUTED PROTEASE VARIANT AND AMYLASE VARIANT-CONTAINING CLEANING COMPOSITIONS
    • 多种取代的蛋白质变体和淀粉含有变化的清洁组合物
    • WO9920723A9
    • 1999-09-02
    • PCT/US9822486
    • 1998-10-23
    • PROCTER & GAMBLEGHOSH CHANCHAL KUMARBAECK ANDRE CESAROHTANI RYOHEIBUSCH ALFREDSHOWELL MICHAEL STANTON
    • GHOSH CHANCHAL KUMARBAECK ANDRE CESAROHTANI RYOHEIBUSCH ALFREDSHOWELL MICHAEL STANTON
    • A23G4/00A23G4/12A23K1/165A61K8/00A61K8/25A61K8/36A61K8/37A61K8/40A61K8/41A61K8/44A61K8/46A61K8/60A61K8/64A61K8/66A61Q1/02A61Q1/14A61Q11/00A61Q11/02A61Q19/00A61Q19/10C11D3/386C11D3/395C11D7/42C12N1/15C12N1/19C12N1/21C12N5/10C12N9/50C12N9/54C12N9/56C12N9/58C12N15/09C12N15/57C12R1/07C11D
    • C12N9/54A23G4/123A23K20/189A61K8/66A61K2800/86A61Q11/00A61Q19/00C11D3/386C11D3/38609C11D3/38618C11D3/3907C11D3/3945C12Y304/00C12Y304/21062
    • The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; an amylase variant and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
    • 本发明涉及包含蛋白酶变体的清洁组合物。 一种清洁组合物包含蛋白酶变体,其包括氨基酸残基与对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置103的氨基酸残基处的另一个天然存在的氨基酸残基的取代,以及氨基酸残基与另一个天然存在的 在一个或多个对应于1,2,3,4,8,9,10,12,13,16,17,18,19,20,21,22,24,27,33, 37,38,42,43,48,55,57,58,61,62,68,72,75,76,77,78,79,86,87,89,97,98,99,101,102, 104,106,107,109,111,114,116,117,119,121,123,126,128,130,131,133,134,137,140,141,142,146,147, 160,166,167,170,173,174,177,181,182,183,184,185,188,192,194,188,203,204,205,206,209,210,211,212,213, 214,215,216,217,218,222,224,227,228,222,224,227,228,223,232,236,237,238,240,242,243,244,245,246,247,248,249,251,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,222,224,227,218,223,232 253,254,25 5,256,257,258,259,260,261,262,263,265,268,269,270,271,272,274和275的解淀粉芽孢杆菌枯草杆菌蛋白酶; 其中当所述蛋白酶变体包含氨基酸残基在对应于位置103和76的位置上的取代时,还存在氨基酸残基在一个或多个除对应于位置27的氨基酸残基位置之外的一个或多个氨基酸残基位置的取代, 99,101,104,107,109,123,128,166,204,206,210,216,217,218,222,260,265或274的解淀粉芽孢杆菌枯草杆菌蛋白酶; 和一种或多种清洁辅助材料。 另一种清洁组合物包含蛋白酶变体,其包括氨基酸残基与对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置62,212,230,232,252和257的一个或多个氨基酸残基处的另一个天然存在的氨基酸残基取代; 淀粉酶变体和一种或多种清洁辅助材料。 还提供了使用清洁组合物的方法。