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1. 发明申请

WO2011073956A2 HETEROLOGOUS HOSTS 审中-公开
标题翻译：异物宿主
公开(公告)号：WO2011073956A2
公开(公告)日：2011-06-23
申请号：PCT/IB2010/055923
申请日：2010-12-17
申请人： GENE BRIDGES GMBH , ZHANG, Youming
发明人： ZHANG, Youming
IPC分类号： C12P17/10
CPC分类号： C12N15/70 , A61K35/74 , A61K2035/11 , C07D245/02 , C07D417/06 , C07D493/04 , C07D498/22 , C07G17/00 , C07K5/06139 , C07K7/64 , C12N9/1288 , C12N9/90 , C12N9/93 , C12N15/52 , C12N15/74 , C12P17/10 , C12P17/181 , C12Y207/08007 , Y02P20/52
摘要： This invention is related to bacterial engineering and the heterologous expression of useful compounds. In particular, the invention relates to a heterologous host that has been engineered for expression of a gene which is capable of polyketide or non- ribosomal peptide synthesis. Methods of treating cancer are also disclosed.
摘要翻译：本发明涉及细菌工程和有用化合物的异源表达。特别地，本发明涉及已被改造用于表达能够进行聚酮化合物或非核糖体肽合成的基因的异源宿主。还公开了治疗癌症的方法。

2. 发明申请

WO2010140066A2 METHOD OF ALTERING NUCLEIC ACIDS 审中-公开
标题翻译：改变核酸的方法
公开(公告)号：WO2010140066A2
公开(公告)日：2010-12-09
申请号：PCT/IB2010/002396
申请日：2010-06-04
申请人： GENE BRIDGES GMBH , STEWART, Adrian, Francis , IACONE, Roberto , MARESCA, Marcello , ANASTASSIADIS, Konstantinos
发明人： STEWART, Adrian, Francis , IACONE, Roberto , MARESCA, Marcello , ANASTASSIADIS, Konstantinos
CPC分类号： C12N15/102 , C12N15/111 , C12N15/113 , C12N15/90 , C12N2310/124 , C12N2320/50
摘要： The invention provides a method for altering the sequence of a target nucleic acid molecule to incorporate a replacement nucleic acid sequence. The method comprising the steps of: a) introducing a nucleic acid fragment into the target nucleic acid molecule by homologous recombination, wherein the nucleic acid fragment comprises: i) a first region of homology to the target nucleic acid molecule; ii) a 5' splice site; iii) a selectable marker; iv) a 3' splice site; v) a second region of homology to the target nucleic acid molecule; wherein components i) to v) are ordered from 5' to 3'; and the replacement nucleic acid sequence is positioned within or between the first and second regions of homology but external of the splice sites; b) selecting for the introduction of the nucleic acid fragment using the selectable marker; c) incubating the product of step b) under conditions suitable for a splicing reaction to occur such that the selectable marker is excised and from the target nucleic acid, and thereby generating the desired altered target nucleic acid sequence.
摘要翻译：本发明提供了改变靶核酸分子序列以掺入置换核酸序列的方法。该方法包括以下步骤：a）通过同源重组将核酸片段引入靶核酸分子，其中所述核酸片段包含：i）与靶核酸分子同源的第一区域; ii）5'剪接位点; iii）可选标记; iv）3'剪接位点; v）与靶核酸分子同源的第二区域; 其中组分i）至v）从5'到3'被排序; 并且所述置换的核酸序列位于所述第一和第二同源区之内或之间，但位于所述剪接位点的外部; b）使用选择性标记选择引入核酸片段; c）在适合于剪接反应的条件下孵育步骤b）的产物，使得切除选择性标记并从靶核酸中产生所需的改变的靶核酸序列。

3. 发明申请

WO2010113031A3 METHOD OF ALTERING NUCLEIC ACIDS 审中-公开
公开(公告)号：WO2010113031A3
公开(公告)日：2010-10-07
申请号：PCT/IB2010/000893
申请日：2010-03-30
申请人： GENE BRIDGES GMBH , STEWART, Adrian, Francis , ZHANG, Youming , MARESCA, Marcello , KRANZ, Harald , NOLL, Stephan
发明人： STEWART, Adrian, Francis , ZHANG, Youming , MARESCA, Marcello , KRANZ, Harald , NOLL, Stephan
IPC分类号： C12N15/10 , C12N15/90
摘要： The invention provides a method for altering the sequence of a target nucleic acid molecule, said method comprising the steps of: a) introducing a nucleic acid fragment into the target nucleic acid molecule by homologous recombination, wherein the nucleic acid fragment comprises: i) a first region of homology to the target nucleic acid molecule; ii) a first recognition sequence for a Type IIS nuclease; iii) a selectable marker; iv) a second recognition sequence for a Type IIS nuclease; v) a second region of homology to the target nucleic acid molecule; wherein components i) to v) are ordered from 5' to 3'; and a replacement nucleic acid sequence positioned between the first and second regions of homology but flanking the recognition sequences for the Type IIS nucleases; b) selecting for the incorporation of the replacement nucleic acid using the selectable marker, and c) cleaving the product of step b) with Type IIS nucleases such that the selectable marker is excised, to produce a linear target fragment including the desired replacement sequence.

4. 发明申请

WO2010113031A2 METHOD OF ALTERING NUCLEIC ACIDS 审中-公开
标题翻译：改变核酸的方法
公开(公告)号：WO2010113031A2
公开(公告)日：2010-10-07
申请号：PCT/IB2010000893
申请日：2010-03-30
申请人： GENE BRIDGES GMBH , STEWART ADRIAN FRANCIS , ZHANG YOUMING , MARESCA MARCELLO , KRANZ HARALD , NOLL STEPHAN
发明人： STEWART ADRIAN FRANCIS , ZHANG YOUMING , MARESCA MARCELLO , KRANZ HARALD , NOLL STEPHAN
IPC分类号： C12N15/10
CPC分类号： C12N9/22 , C12N15/102 , C12N15/1082 , C12N15/902 , C12N2800/80
摘要： The invention provides a method for altering the sequence of a target nucleic acid molecule, said method comprising the steps of: a) introducing a nucleic acid fragment into the target nucleic acid molecule by homologous recombination, wherein the nucleic acid fragment comprises: i) a first region of homology to the target nucleic acid molecule; ii) a first recognition sequence for a Type IIS nuclease; iii) a selectable marker; iv) a second recognition sequence for a Type IIS nuclease; v) a second region of homology to the target nucleic acid molecule; wherein components i) to v) are ordered from 5' to 3'; and a replacement nucleic acid sequence positioned between the first and second regions of homology but flanking the recognition sequences for the Type IIS nucleases; b) selecting for the incorporation of the replacement nucleic acid using the selectable marker, and c) cleaving the product of step b) with Type IIS nucleases such that the selectable marker is excised, to produce a linear target fragment including the desired replacement sequence.
摘要翻译：本发明提供了改变靶核酸分子序列的方法，所述方法包括以下步骤：a）通过同源重组将核酸片段引入靶核酸分子，其中所述核酸片段包含：i）与靶核酸分子同源的第一区域; ii）类型IIS核酸酶的第一识别序列; iii）可选标记; iv）类型IIS核酸酶的第二识别序列; v）与靶核酸分子同源的第二区域; 其中组分i）至v）从5'到3'被排序; 和位于同源性的第一和第二区之间但侧翼于IIS型核酸酶的识别序列的置换核酸序列; b）选择使用所述选择性标记并入所述置换的核酸，以及c）用所述IIS核酸酶切割步骤b）的产物，从而切除所述选择性标记，以产生包括所需替换序列的线性靶片段。

5. 发明申请

WO2010049807A3 T7 DUAL PROMOTER-BASED VECTOR SYSTEM FOR RECOMBINANT EXPRESSION OF POLYPEPTIDES 审中-公开
标题翻译： T7双重促进剂的多聚体表达载体系统
公开(公告)号：WO2010049807A3
公开(公告)日：2010-08-26
申请号：PCT/IB2009007384
申请日：2009-10-29
申请人： GENE BRIDGES GMBH , ZHANG YOUMING
发明人： ZHANG YOUMING
IPC分类号： C12N15/73
CPC分类号： C12N15/73
摘要： A two vector system for broad host range inducible expression of a polypeptide of interest comprising: i). a first vector comprising: (a) the T7 promoter positioned 5' of an inducible promoter; (b) a cloning site positioned 3' of the inducible promoter for insertion of a gene encoding the polypeptide of interest; and ii). a second vector for making a host cell competent for T7 RNA polymerase expression, wherein the vector comprises a gene encoding the T7 RNA polymerase under the control of an inducible promoter.
摘要翻译：用于广泛宿主范围的两种载体系统诱导性表达感兴趣的多肽，包括：i）。第一载体包含：（a）位于诱导型启动子5'的T7启动子; （b）位于诱导型启动子3'的克隆位点，用于插入编码目标多肽的基因; 和ii）。用于制备用于T7RNA聚合酶表达的宿主细胞的第二载体，其中所述载体包含编码在诱导型启动子控制下的T7RNA聚合酶的基因。

6. 发明申请

WO2002062988A2 RECOMBINATION METHOD 审中-公开
标题翻译：重组方法
公开(公告)号：WO2002062988A2
公开(公告)日：2002-08-15
申请号：PCT/IB2002/001415
申请日：2002-02-08
申请人： GENE BRIDGES GMBH , MUIJRERS, Joep, P., P. , ZHANG, Youming , STEWART, Adrian, Francis
发明人： MUIJRERS, Joep, P., P. , ZHANG, Youming , STEWART, Adrian, Francis
IPC分类号： C12N15/00
CPC分类号： C12N15/102
摘要： The invention relates to a novel method for altering the sequence of a nucleic acid molecule using repair recombination in a simple one component system. The frequency of the recombination reaction is high, allowing a range of feasible selection strategies to identify successful recombination events. The method involves the steps of bringing a first nucleic acid molecule into contact with a second nucleic acid molecule in the presence of a phage annealing protein into contact with a second nucleic acid molecule in the presence of a phage annealing protein, or a functional equivalent or fragment thereof, wherein said first nucleic acid molecule comprises at least two regions of shared sequence homology with the second nucleic acid molecule, under conditions suitable for repair recombination to occur between said first and second nucleic acid molecules; ad selecting a nucleic acid molecule whose sequence has been altered so as to include sequence from said second nucleic acid molecule.
摘要翻译：本发明涉及一种在简单的单一成分系统中使用修复重组改变核酸分子的序列的新方法。重组反应的频率很高，允许一系列可行的选择策略来鉴定成功的重组事件。该方法包括以下步骤：在噬菌体退火蛋白存在下，使第一核酸分子与第二核酸分子接触，以在噬菌体退火蛋白或功能等价物或存在下与第二核酸分子接触，其中所述第一核酸分子在适于在所述第一和第二核酸分子之间发生修复重组的条件下包含与所述第二核酸分子具有共享序列同源性的至少两个区域; 选择其序列已被改变以便包括来自所述第二核酸分子的序列的核酸分子。

7. 发明申请

WO2017174753A1 ADENOVIRAL VECTORS 审中-公开
标题翻译：腺病毒载体
公开(公告)号：WO2017174753A1
公开(公告)日：2017-10-12
申请号：PCT/EP2017/058306
申请日：2017-04-06
申请人： GENE BRIDGES GMBH
发明人： STEWART, Adrian, Francis , FU, Jun , EHRHARDT, Anja , EHRKE-SCHULZ, Eric , ZHANG, Wenli
IPC分类号： C12N15/86 , C07K14/005
摘要： The invention relates to adenoviral vectors, cells for use in generating adenoviral vectors, methods for generating adenoviral vectors, and therapeutic uses of adenoviral vectors in gene therapy, tumour therapy and as vaccines.
摘要翻译：本发明涉及腺病毒载体，用于产生腺病毒载体的细胞，产生腺病毒载体的方法以及腺病毒载体在基因治疗，肿瘤治疗和疫苗中的治疗用途。

8. 发明申请

WO2014060427A3 ELECTROCOMPETENT CELLS AND PREPARATION THEREOF 审中-公开
标题翻译：电解质细胞及其制备
公开(公告)号：WO2014060427A3
公开(公告)日：2014-07-03
申请号：PCT/EP2013071546
申请日：2013-10-15
申请人： UNIV SAARLAND , GENE BRIDGES GMBH , UNIV DRESDEN TECH
发明人： MUELLER ROLF , TU QIANG , ZHANG YOUMING , STEWART FRANCIS , FU JUN
IPC分类号： C12N1/20 , C12N15/87
CPC分类号： C12N15/743 , C12N1/20 , C12N15/70 , C12N15/74
摘要： The present invention provides a method of preparing electrocompetent cells from Gram-negative bacteria characterized in that the bacteria are prepared under room temperature. Also provided are electrocompetent Gram-negative bacteria and kits which comprise the electrocompetent bacteria.
摘要翻译：本发明提供了由革兰氏阴性菌制备电感受态细胞的方法，其特征在于所述细菌在室温下制备。还提供了电感受态革兰氏阴性细菌和包含电感受态细菌的试剂盒。

9. 发明申请

WO2011073956A3 HETEROLOGOUS HOSTS 审中-公开
标题翻译：异体宿主
公开(公告)号：WO2011073956A3
公开(公告)日：2012-01-12
申请号：PCT/IB2010055923
申请日：2010-12-17
申请人： GENE BRIDGES GMBH , ZHANG YOUMING , FU JUN , BIAN XIAOYING , STEWART FRANCIS , MUELLER ROLF
发明人： ZHANG YOUMING , FU JUN , BIAN XIAOYING , STEWART FRANCIS , MUELLER ROLF
IPC分类号： C12P17/10 , A61K35/74 , C07D417/06 , C07D493/04 , C12N9/00 , C12N9/90 , C12N15/52 , C12N15/87 , C12P17/18
CPC分类号： C12N15/70 , A61K35/74 , A61K2035/11 , C07D245/02 , C07D417/06 , C07D493/04 , C07D498/22 , C07G17/00 , C07K5/06139 , C07K7/64 , C12N9/1288 , C12N9/90 , C12N9/93 , C12N15/52 , C12N15/74 , C12P17/10 , C12P17/181 , C12Y207/08007 , Y02P20/52
摘要： This invention is related to bacterial engineering and the heterologous expression of useful compounds. In particular, the invention relates to a heterologous host that has been engineered for expression of a gene which is capable of polyketide or non- ribosomal peptide synthesis. Methods of treating cancer are also disclosed.
摘要翻译：本发明涉及细菌工程和有用化合物的异源表达。特别地，本发明涉及已被改造用于表达能够进行聚酮化合物或非核糖体肽合成的基因的异源宿主。还公开了治疗癌症的方法。

10. 发明申请

WO2011154927A9 DIRECT CLONING 审中-公开
公开(公告)号：WO2011154927A9
公开(公告)日：2011-12-15
申请号：PCT/IB2011/052549
申请日：2011-06-10
申请人： GENE BRIDGES GMBH , ZHANG, Youming , FU, Jun , STEWART, Francis
发明人： ZHANG, Youming , FU, Jun , STEWART, Francis
IPC分类号： C12N15/90 , C12N9/22
摘要： A method for performing homologous recombination between at least a first nucleic acid molecule and a second nucleic acid molecule which share at least one region of sequence homology. A method for improving the efficiency of homologous recombination.

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