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1. 发明申请

WO2010140066A2 METHOD OF ALTERING NUCLEIC ACIDS 审中-公开
标题翻译：改变核酸的方法
公开(公告)号：WO2010140066A2
公开(公告)日：2010-12-09
申请号：PCT/IB2010/002396
申请日：2010-06-04
申请人： GENE BRIDGES GMBH , STEWART, Adrian, Francis , IACONE, Roberto , MARESCA, Marcello , ANASTASSIADIS, Konstantinos
发明人： STEWART, Adrian, Francis , IACONE, Roberto , MARESCA, Marcello , ANASTASSIADIS, Konstantinos
CPC分类号： C12N15/102 , C12N15/111 , C12N15/113 , C12N15/90 , C12N2310/124 , C12N2320/50
摘要： The invention provides a method for altering the sequence of a target nucleic acid molecule to incorporate a replacement nucleic acid sequence. The method comprising the steps of: a) introducing a nucleic acid fragment into the target nucleic acid molecule by homologous recombination, wherein the nucleic acid fragment comprises: i) a first region of homology to the target nucleic acid molecule; ii) a 5' splice site; iii) a selectable marker; iv) a 3' splice site; v) a second region of homology to the target nucleic acid molecule; wherein components i) to v) are ordered from 5' to 3'; and the replacement nucleic acid sequence is positioned within or between the first and second regions of homology but external of the splice sites; b) selecting for the introduction of the nucleic acid fragment using the selectable marker; c) incubating the product of step b) under conditions suitable for a splicing reaction to occur such that the selectable marker is excised and from the target nucleic acid, and thereby generating the desired altered target nucleic acid sequence.
摘要翻译：本发明提供了改变靶核酸分子序列以掺入置换核酸序列的方法。该方法包括以下步骤：a）通过同源重组将核酸片段引入靶核酸分子，其中所述核酸片段包含：i）与靶核酸分子同源的第一区域; ii）5'剪接位点; iii）可选标记; iv）3'剪接位点; v）与靶核酸分子同源的第二区域; 其中组分i）至v）从5'到3'被排序; 并且所述置换的核酸序列位于所述第一和第二同源区之内或之间，但位于所述剪接位点的外部; b）使用选择性标记选择引入核酸片段; c）在适合于剪接反应的条件下孵育步骤b）的产物，使得切除选择性标记并从靶核酸中产生所需的改变的靶核酸序列。

2. 发明申请

WO2012168167A1 METHOD FOR DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO VASCULAR BED CELLS 审中-公开
标题翻译：将多发性干细胞分化成血管细胞的方法
公开(公告)号：WO2012168167A1
公开(公告)日：2012-12-13
申请号：PCT/EP2012/060461
申请日：2012-06-04
申请人： F. HOFFMANN-LA ROCHE AG , CHRISTENSEN, Klaus , GRAF, Martin , IACONE, Roberto , PATSCH, Christoph , THOMA, Eva, Christina
发明人： CHRISTENSEN, Klaus , GRAF, Martin , IACONE, Roberto , PATSCH, Christoph , THOMA, Eva, Christina
IPC分类号： C12N5/078
CPC分类号： G01N33/5088 , C12N5/069 , C12N5/0691 , C12N2501/01 , C12N2501/165 , C12N2501/41 , C12N2501/415 , C12N2501/727 , C12N2501/999 , C12N2506/02 , C12N2506/45
摘要： This application relates to a method for differentiating pluripotent stem cells (PSCs) into vascular bed cells. Moreover this application relates to a method for differentiating human embryonic stem cells (ESCs) and induced pluripotent stem cells (i PSCs) into vascular bed cells based on linked steps of chemically defined medium inductions.
摘要翻译：本申请涉及将多能干细胞（PSC）分化成血管床细胞的方法。此外，本申请涉及一种基于化学上定义的介质诱导物的连接步骤将人类胚胎干细胞（ESCs）和诱导的多能干细胞（i PSCs）分化成血管床细胞的方法。

3. 发明申请

WO2012022725A2 CONVERSION OF SOMATIC CELLS TO INDUCED REPROGRAMMED NEURAL STEM CELLS (IRNSCS) 审中-公开
标题翻译：体细胞转化为诱导重新编程的神经干细胞（IRNSCS）
公开(公告)号：WO2012022725A2
公开(公告)日：2012-02-23
申请号：PCT/EP2011/064051
申请日：2011-08-16
申请人： F. HOFFMANN-LA ROCHE AG , CHRISTENSEN, Klaus , GRAF, Martin , IACONE, Roberto , JAGASIA, Ravi
发明人： CHRISTENSEN, Klaus , GRAF, Martin , IACONE, Roberto , JAGASIA, Ravi
IPC分类号： C12N5/0797
CPC分类号： C12N5/0623 , A61K35/30 , C12N15/86 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/13 , C12N2501/405 , C12N2501/60 , C12N2501/602 , C12N2501/727 , C12N2506/1307 , C12N2510/00
摘要： This application relates to a method for converting somatic cells to Neural Stem Cells (NSCs). Moreover this application relates to a method for converting human fibroblasts, keratinocytes or adipocytes to neural stem cells based on linked steps of genes transduction and chemically defined medium induction.
摘要翻译：本申请涉及用于将体细胞转化为神经干细胞（NSC）的方法。此外，本申请涉及基于基因转导和化学上确定的培养基诱导的连接步骤将人成纤维细胞，角质形成细胞或脂肪细胞转化为神经干细胞的方法。

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