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1. 发明申请

WO2002062988A2 RECOMBINATION METHOD 审中-公开
标题翻译：重组方法
公开(公告)号：WO2002062988A2
公开(公告)日：2002-08-15
申请号：PCT/IB2002/001415
申请日：2002-02-08
申请人： GENE BRIDGES GMBH , MUIJRERS, Joep, P., P. , ZHANG, Youming , STEWART, Adrian, Francis
发明人： MUIJRERS, Joep, P., P. , ZHANG, Youming , STEWART, Adrian, Francis
IPC分类号： C12N15/00
CPC分类号： C12N15/102
摘要： The invention relates to a novel method for altering the sequence of a nucleic acid molecule using repair recombination in a simple one component system. The frequency of the recombination reaction is high, allowing a range of feasible selection strategies to identify successful recombination events. The method involves the steps of bringing a first nucleic acid molecule into contact with a second nucleic acid molecule in the presence of a phage annealing protein into contact with a second nucleic acid molecule in the presence of a phage annealing protein, or a functional equivalent or fragment thereof, wherein said first nucleic acid molecule comprises at least two regions of shared sequence homology with the second nucleic acid molecule, under conditions suitable for repair recombination to occur between said first and second nucleic acid molecules; ad selecting a nucleic acid molecule whose sequence has been altered so as to include sequence from said second nucleic acid molecule.
摘要翻译：本发明涉及一种在简单的单一成分系统中使用修复重组改变核酸分子的序列的新方法。重组反应的频率很高，允许一系列可行的选择策略来鉴定成功的重组事件。该方法包括以下步骤：在噬菌体退火蛋白存在下，使第一核酸分子与第二核酸分子接触，以在噬菌体退火蛋白或功能等价物或存在下与第二核酸分子接触，其中所述第一核酸分子在适于在所述第一和第二核酸分子之间发生修复重组的条件下包含与所述第二核酸分子具有共享序列同源性的至少两个区域; 选择其序列已被改变以便包括来自所述第二核酸分子的序列的核酸分子。

2. 发明申请

WO2003010322A1 IMPROVED RECT OR RECET CLONING AND SUBCLONING METHOD 审中-公开
标题翻译：改进的重建或恢复克隆和扩充方法
公开(公告)号：WO2003010322A1
公开(公告)日：2003-02-06
申请号：PCT/EP2002/008096
申请日：2002-07-19
申请人： ZHANG, Youming , STEWART, Francis, A. , MUIJRERS, Joep, P., P.
发明人： ZHANG, Youming , STEWART, Francis, A. , MUIJRERS, Joep, P., P.
IPC分类号： C12N15/90
CPC分类号： C12N15/64 , C12N15/66
摘要： The invention refers to an improved method for DNA cloning and subcloning using Rec T or RecET-mediated homologous recombination. Further, novel reagent kits suitable for carrying out the method are provided.
摘要翻译：本发明涉及使用Rec T或RecET介导的同源重组的DNA克隆和亚克隆的改进方法。此外，提供了适用于实施该方法的新型试剂盒。

3. 发明申请

WO2002062988A3 RECOMBINATION METHOD 审中-公开
公开(公告)号：WO2002062988A3
公开(公告)日：2002-08-15
申请号：PCT/IB2002/001415
申请日：2002-02-08
申请人： GENE BRIDGES GMBH , MUIJRERS, Joep, P., P. , ZHANG, Youming , STEWART, Adrian, Francis
发明人： MUIJRERS, Joep, P., P. , ZHANG, Youming , STEWART, Adrian, Francis
IPC分类号： C12N15/00
摘要： The invention relates to a novel method for altering the sequence of a nucleic acid molecule using repair recombination in a simple one component system. The frequency of the recombination reaction is high, allowing a range of feasible selection strategies to identify successful recombination events. The method involves the steps of bringing a first nucleic acid molecule into contact with a second nucleic acid molecule in the presence of a phage annealing protein into contact with a second nucleic acid molecule in the presence of a phage annealing protein, or a functional equivalent or fragment thereof, wherein said first nucleic acid molecule comprises at least two regions of shared sequence homology with the second nucleic acid molecule, under conditions suitable for repair recombination to occur between said first and second nucleic acid molecules; ad selecting a nucleic acid molecule whose sequence has been altered so as to include sequence from said second nucleic acid molecule.

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