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    • 9. 发明申请
    • METHOD FOR ENCODING AND SCREENING COMBINATORIAL LIBRARIES
    • 编辑和筛选组合图书馆的方法
    • WO2007070761A2
    • 2007-06-21
    • PCT/US2006/061728
    • 2006-12-07
    • COLORADO SEMINARY, WHICH OWNS AND OPERATES THE UNIVERSITY OF DENVERKUTATELADZE, Andrei G.KOTTANI, RudreshaVALIULIN, RomanMAJJIGAPU, Janaki
    • KUTATELADZE, Andrei G.KOTTANI, RudreshaVALIULIN, RomanMAJJIGAPU, Janaki
    • C40B30/06
    • C40B30/06
    • A method of screening a library comprising: (i) providing either (a) a library comprising more than one copy of different library members, each copy of a different library member attached to a different releasable tag through a releasable covalent bond; where a plurality of tags uniquely encode each library member; or (b) a library comprising one or more copies of a library member attached to a support, with a plurality of tags uniquely encoding each library member; or (c) a library comprising different library members, each different library member attached to a plurality of tags uniquely encoding the different library member; (ii) providing a target compound with tethered sensitizer in specific binding proximity to the library, allowing specific binding of the target compound with tethered sensitizer to a selected library member; (iii) exciting the tethered sensitizer with excitation photoradiation, whereby the releasable tags attached to the selected library member are released; and (iv) detecting the releasable tags.
    • 一种筛选文库的方法,包括:(i)提供(a)包含多于一个不同文库成员的文库的文库,通过可释放共价键连接到不同可释放标签的不同文库成员的每个拷贝; 其中多个标签唯一地编码每个库成员; 或(b)包括连接到支持的库成员的一个或多个副本的库,具有唯一编码每个库成员的多个标签; 或(c)包含不同库成员的库,每个不同的库成员附加到唯一编码不同库成员的多个标签; (ii)提供具有特异性结合到文库的具有束缚敏化剂的靶化合物,允许目标化合物与拴系的敏化剂特异性结合到选定的文库成员; (iii)通过激发光激发激发拴系的敏化剂,从而释放附着在所选文库构件上的可释放标签; 和(iv)检测可释放标签。