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81. 发明申请

WO2006110855A2 METHODS FOR DETERMINING SEQUENCE VARIANTS USING ULTRA-DEEP SEQUENCING 审中-公开
标题翻译：使用超深度序列测定序列变量的方法
公开(公告)号：WO2006110855A2
公开(公告)日：2006-10-19
申请号：PCT/US2006/013753
申请日：2006-04-12
申请人： 454 LIFE SCIENCES CORPORATION , LEAMON, John, Harris , LEE, William, Lun , SIMONS, Jan, Fredrick , DESANY, Brian , RONAN, Mike, Todd , DRAKE, James , LOHMAN, Kenton , EGHOLM, Michael , ROTHBERG, Jonathan
发明人： LEAMON, John, Harris , LEE, William, Lun , SIMONS, Jan, Fredrick , DESANY, Brian , RONAN, Mike, Todd , DRAKE, James , LOHMAN, Kenton , EGHOLM, Michael , ROTHBERG, Jonathan
CPC分类号： C12Q1/6858 , C12Q1/6869 , C12Q2565/537 , C12Q2565/515 , C12Q2545/114
摘要： The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.
摘要翻译：所要求保护的发明提供了使用焦磷酸测序技术直接测序PCR产物的新的样品制备方法。 PCR产物可以是基因组的特定区域。本公开中提供的技术允许一个个体或个体群体中的单个等位基因多态性的SNP（单核苷酸多态性）检测，分类和评估。结果可用于患者的诊断和治疗以及病毒和细菌群体鉴定的评估。

82. 发明申请

WO2006083305A2 METHODS AND COMPOSITIONS FOR THE DETECTION OF BIOLOGICAL MOLECULES USING A TWO PARTICLE COMPLEX 审中-公开
标题翻译：使用两个颗粒复合物检测生物分子的方法和组合物
公开(公告)号：WO2006083305A2
公开(公告)日：2006-08-10
申请号：PCT/US2005022388
申请日：2005-06-23
申请人： BARD ALLEN , MIAO WUJIAM
发明人： BARD ALLEN , MIAO WUJIAM
CPC分类号： G01N33/582 , C12Q1/6802 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , C12Q1/6837 , G01N33/532 , G01N33/54313 , G01N33/54373 , C12Q2565/515 , C12Q2563/143 , C12Q2563/137 , C12Q2563/103
摘要： The invention provides methods of detecting analytes of interest in a sample using electrogenerated chemiluminescence. The invention also provides compositions comprising at least one solid support that entraps or contains an electrogenerated chemiluminescent moiety.
摘要翻译：本发明提供使用电生化学发光检测样品中感兴趣的分析物的方法。本发明还提供了包含至少一种固体支持物的组合物，其夹带或包含电致化学发光部分。

83. 发明申请

WO2006028517A2 COMPARATIVE GENOMIC RESEQUENCING 审中-公开
标题翻译：比较基因组测序
公开(公告)号：WO2006028517A2
公开(公告)日：2006-03-16
申请号：PCT/US2005/012136
申请日：2005-04-08
申请人： NIMBLEGEN SYSTEMS, INC. , GREEN, Roland, D. , SMITH, Steve , ALBERT, Thomas, J. , NUWAYSIR, Emilie, F.
发明人： GREEN, Roland, D. , SMITH, Steve , ALBERT, Thomas, J. , NUWAYSIR, Emilie, F.
IPC分类号： C12M1/34 , C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6827 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , C12Q2600/156 , Y10S977/791 , Y10S977/795 , C12Q2565/515 , C12Q2539/101
摘要： The present invention is an improved method of resequencing DNA using microarrays to rapidly map and identify SNPs, deletions and amplification events present in the genome of an organism. The method is performed by hybridizing a reference and a test genome to two separate arrays with each array exhibiting a specific intensity pattern. The intensity differences between the reference and the test genome arrays are used to produce a mutation map. The mapped differences are resequenced on a set of resequencing arrays to identify specific genetic mutations.
摘要翻译：本发明是使用微阵列重新测序DNA以改进生物体基因组中存在的SNP，缺失和扩增事件的方法和程序的改进方法。该方法通过将参照和测试基因组与两个分开的阵列杂交来进行，每个阵列表现出特定的强度模式。参考和测试基因组阵列之间的强度差异用于产生突变图。映射的差异在一组重排序阵列上重新排序，以确定特定的基因突变。

84. 发明申请

WO0161036A8 A METHOD OF MAPPING RESTRICTION ENDONUCLEASE CLEAVAGE SITES 审中-公开
标题翻译：映射限制性内切酶CLEAVAGE SITES的方法
公开(公告)号：WO0161036A8
公开(公告)日：2004-04-15
申请号：PCT/GB0100718
申请日：2001-02-19
申请人： COMPLETE GENOMICS AS , LEXOW PREBEN , TOWLER PHILIP DEAN
发明人： LEXOW PREBEN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/683 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/525 , C12Q2565/515 , C12Q2525/191 , C12Q2525/131
摘要： The invention provides a two-step sorting procedure where it is possible to scan the overhanging single-stranded ends of nucleic acid fragments quickly and efficiently using solid supports, such as microarrays. Use is made of two different sets of degenerate overhang-adaptors in this regard. The invention also provides new methods and strategies inter alia for collecting information about sequences and cleavage sites that are between the cleavage sites that have generated an overhang pair. An effective method of producing the restriction map, making it easier to create multiple maps, is also described.
摘要翻译：本发明提供两步分选程序，其中可以使用固体支持物（例如微阵列）快速有效地扫描核酸片段的突出单链末端。在这方面使用两套不同的退化突出接头。本发明还提供了新的方法和策略，特别是用于收集关于产生突出对的切割位点之间的序列和切割位点的信息。还描述了生产限制图的有效方法，使得更容易创建多个映射。

85. 发明申请

WO02099423A3 METHOD FOR DETERMINING AN ANALYTE 审中-公开
标题翻译：方法用于测定分析
公开(公告)号：WO02099423A3
公开(公告)日：2003-09-18
申请号：PCT/EP0206003
申请日：2002-05-31
申请人： NANOTYPE GMBH , BLANK KERSTIN , SPINNLER KATRIN , MAI THAO , OESTERHELT FILIPP , GILBERT ILKA , STEIPE BORIS , GAUB HERMANN , ALBRECHT CHRISTIAN , BRINK GUNNAR
发明人： BLANK KERSTIN , SPINNLER KATRIN , MAI THAO , OESTERHELT FILIPP , GILBERT ILKA , STEIPE BORIS , GAUB HERMANN , ALBRECHT CHRISTIAN , BRINK GUNNAR
IPC分类号： C12Q1/68 , G01N33/543 , G01N33/53
CPC分类号： G01N33/54366 , C12Q1/6837 , C12Q2565/515
摘要： The invention relates to a method for determining an analyte in a sample. According to said method, a probe is transferred from a plunger onto an analyte that is bound to a support material. The method is particularly suitable for micro-arrays. The invention also relates to a device for carrying out said method.
摘要翻译：本发明涉及为s过程对于其中探头从邮票上发送一个键合到背衬分析物的分析物的样品中的确定。该方法特别适用于微阵列。本发明还涉及一种用于实施该方法的装置。

86. 发明申请

WO03057901A2 METHOD FOR DETECTING NUCLEIC ACID MOLECULES BASED ON THE RELATIVE MOVEMENT OF SURFACES 审中-公开
标题翻译：基于表面相对运动检测核酸分子的方法
公开(公告)号：WO03057901A2
公开(公告)日：2003-07-17
申请号：PCT/US0300268
申请日：2003-01-06
申请人： INTEGRATED NANO TECH LLC , CONNOLLY DENNIS M , DEBOER CHARLES D , POTTER MICHAEL D
发明人： CONNOLLY DENNIS M , DEBOER CHARLES D , POTTER MICHAEL D
IPC分类号： C12Q1/68 , G01N3/00 , G01N3/06 , G01N11/16 , C12Q
CPC分类号： C12Q1/6837 , G01N11/16 , G01N2203/0025 , G01N2203/0623 , C12Q2565/515 , C12Q2565/543
摘要： The present invention relates to methods for detecting a target nucleic acid molecule in a sample. The methods involve providing a device having surfaces not in contact with each other, at least one of which is moveable. A pair of oligonucleotide probes complementary to the target nucleic acid molecule are attached to the surfaces of the device. The device is then contacted with a sample, under selective hybridization conditions, to permit target molecules, if any, present in the sample, to hybridize to the probes. Finally, it is determined whether there is a decrease in the relative movement between the surfaces, thereby indicating the presence of the target molecule in the sample. Alternatively, single stranded nucleic acid molecules are attached to the surfaces to determine whether the two surfaces are closer to one another than prior to the contacting. Devices used to carry out the above methods are also disclosed.
摘要翻译：本发明涉及检测样品中靶核酸分子的方法。所述方法包括提供具有彼此不接触的表面的装置，其中至少一个是可移动的。与靶核酸分子互补的一对寡核苷酸探针附着到装置的表面。然后在选择性杂交条件下将该装置与样品接触，以允许存在于样品中的靶分子（如果有的话）与探针杂交。最后，确定表面之间的相对移动是否有减少，从而表明样品中目标分子的存在。或者，单链核酸分子连接到表面以确定两个表面是否比接触之前彼此更接近。还公开了用于执行上述方法的装置。

87. 发明申请

WO2003029490A1 METHODS AND ARRAYS FOR DETECTING BIOLOGICAL MOLECULES 审中-公开
标题翻译：检测生物分子的方法和阵列
公开(公告)号：WO2003029490A1
公开(公告)日：2003-04-10
申请号：PCT/US2002/019346
申请日：2002-06-18
申请人： WANG, Yingjian
发明人： WANG, Yingjian
IPC分类号： C12Q1/68
CPC分类号： G01N33/6842 , B01J19/0046 , B01J2219/00387 , B01J2219/00497 , B01J2219/0052 , B01J2219/00527 , B01J2219/00576 , B01J2219/00659 , B01J2219/00673 , B01J2219/00675 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/00743 , B82Y30/00 , C07B2200/11 , C12Q1/6837 , C40B40/00 , G01N33/5005 , G01N33/54306 , G01N33/54366 , G01N33/6854 , C12Q2565/515
摘要： Arrays and methods for detecting one or more biological molecules, where the methods generally comprise the steps of: providing a first support immobilized with one or more reagents; providing a second support immobilized with one on more of ligands; contacting the reagents immobilized to the first support with the ligands immobilized on the second support whereby one or more of the reagents bind to one or more of the ligands; and separating the first support from the second support so that one or more of the bound reagents remain bound to one or more ligands on the second support after separation. In one preferred method, proteins are immobilized on a support with adequate strength so that the proteins can be dissociated from the support under certain conditions, such as after binding with other proteins immobilized on another support. For example, antibody arrays produced according to the present invention may be used to detect protein expressions in a protein lysate and may be used in immunostaining to reveal the presence and location of proteins in cells.
摘要翻译：用于检测一种或多种生物分子的阵列和方法，其中所述方法通常包括以下步骤：提供固定有一种或多种试剂的第一载体; 提供固定有更多配体上的一个的第二载体; 使固定在第一载体上的试剂与固定在第二载体上的配体接触，由此一种或多种试剂与一种或多种配体结合; 以及将第一载体与第二载体分离，使得一个或多个结合的试剂在分离后保持与第二载体上的一种或多种配体结合。在一种优选的方法中，将蛋白质固定在具有足够强度的载体上，使得蛋白质可以在某些条件下，例如在与另一种载体上固定的其它蛋白质结合后，从支持物解离。例如，根据本发明产生的抗体阵列可用于检测蛋白质裂解物中的蛋白质表达，并且可用于免疫染色以揭示蛋白质在细胞中的存在和位置。

88. 发明申请

WO2002099423A2 VERFAHREN ZUR BESTIMMUNG EINES ANALYTEN 审中-公开
标题翻译：方法用于测定分析
公开(公告)号：WO2002099423A2
公开(公告)日：2002-12-12
申请号：PCT/EP2002/006003
申请日：2002-05-31
申请人： NANOTYPE GMBH , BLANK, Kerstin , SPINNLER, Katrin , MAI, Thao , OESTERHELT, Filipp , GILBERT, Ilka , STEIPE, Boris , GAUB, Hermann , ALBRECHT, Christian , BRINK, Gunnar
发明人： BLANK, Kerstin , SPINNLER, Katrin , MAI, Thao , OESTERHELT, Filipp , GILBERT, Ilka , STEIPE, Boris , GAUB, Hermann , ALBRECHT, Christian , BRINK, Gunnar
IPC分类号： G01N33/53
CPC分类号： G01N33/54366 , C12Q1/6837 , C12Q2565/515
摘要： Die vorliegende Erfindung betrifft en Verfahren zur Bestimmung eines Analyten in einer Probe, bei dem eine Sonde von einem Stempel auf einen an eine Unterlage gebundenen Analyten übertragen wird. Das Verfahren ist insbesondere für Microarrays geeignet. Die Erfindung betrifft auch eine Vorrichtung zur Durchführung des Verfahrens.
摘要翻译：本发明涉及为s过程对于其中探头从邮票上发送一个键合到背衬分析物的分析物的样品中的确定。该方法特别适用于微阵列。本发明还涉及一种用于实施该方法的装置。

89. 发明申请

WO0198534A3 MATRIX SCREENING METHOD 审中-公开
标题翻译：矩阵筛选方法
公开(公告)号：WO0198534A3
公开(公告)日：2002-05-30
申请号：PCT/GB0102831
申请日：2001-06-22
申请人： DIVERSYS LTD , HOLT LUCY JESSICA , TOMLINSON IAN
发明人： HOLT LUCY JESSICA , TOMLINSON IAN
IPC分类号： G01N33/50 , B01J19/00 , B01L3/02 , C07K16/18 , C12N15/09 , C12N15/10 , C12Q1/02 , C12Q1/68 , C12Q1/70 , C40B30/04 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/15 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/68 , B01L3/00
CPC分类号： C40B30/04 , B01J19/0046 , B01J2219/00364 , B01J2219/0038 , B01J2219/00387 , B01J2219/0052 , B01J2219/00531 , B01J2219/00533 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00668 , B01J2219/00677 , B01J2219/00691 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , B01L3/0244 , B01L2400/0406 , B82Y30/00 , C07K16/18 , C07K2317/21 , C07K2317/622 , C12N15/1055 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/54366 , G01N33/6842 , G01N33/6845 , C12Q2565/515
摘要： The invention concerns a method which can be used to screen two or more repertoires of molecules against one another and/or to create combinatorial repertoires by combining two or more repertoires. In particular, the invention relates to a method whereby two repertoires of molecules can be screened such that substantially all members of the first repertoire are tested against substantially all members of the second repertoire for functional interactions. Furthermore, the invention relates to the creation and screening of antibody repertoires by combining a repertoire of heavy chains with a repertoire of light chains such that antibodies formed by the substantially all combinations of heavy and light chains can be screened against one or more target ligands.
摘要翻译：本发明涉及一种方法，其可用于通过组合两个或更多个谱组来筛选分子的两个或多个谱系和/或组合组合谱。特别地，本发明涉及一种可以筛选分子的两个谱系的方法，使得基本上所有的第一个谱系的所有成员都被测试了基本上所有的第二个谱的所有成员的功能相互作用。此外，本发明涉及通过将重链的谱系与轻链的所有组成相结合来产生和筛选抗体库，使得由重链和轻链的基本上所有组合形成的抗体可以针对一种或多种靶配体进行筛选。

90. 发明申请

WO02004676A2 COMPOSITE AND AVERAGED HYBRIDIZATIONS 审中-公开
标题翻译：复合和平均混合
公开(公告)号：WO02004676A2
公开(公告)日：2002-01-17
申请号：PCT/US2001/021382
申请日：2001-07-05
IPC分类号： C12Q1/68 , G06F19/20
CPC分类号： G06F19/20 , C12Q1/6837 , C12Q2565/515 , C12Q2545/113
摘要： Composite hybridization arrays and averaged hybridization arrays are provided. Composite hybridization arrays are formed a user selected set of hybridization arrays, and once instantiated in a hybridization array database, are available for searching, analysis, and other data processing as with other types of hybridization arrays. This allows otherwise experiments using multiple different nucleotide microarrays to be efficiently consolidated and analyzed. Averaged hybridization array provide correctly averaged values from multiple user selected dual channel hybridization arrays.
摘要翻译：提供了复合杂交阵列和平均杂交阵列。复合杂交阵列形成用户选择的杂交阵列，并且一旦在杂交阵列数据库中实例化，可用于搜索，分析和其他数据处理，与其他类型的杂交阵列一样。这允许其他实验使用多个不同的核苷酸微阵列进行有效的巩固和分析。平均杂交阵列提供来自多个用户选择的双通道杂交阵列的正确平均值。

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