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71. 发明申请

WO2008135382A3 METHOD FOR THE INTENSIVE EXTRACTION OF CELLULAR COMPOUNDS FROM MICRO-ORGANISMS BY CONTINUOUS CULTURE AND EXTRACTION, AND CORRESPONDING DEVICE 审中-公开
标题翻译：通过连续文化和萃取强化提取微量有机化合物的方法及相关装置
公开(公告)号：WO2008135382A3
公开(公告)日：2009-01-08
申请号：PCT/EP2008054869
申请日：2008-04-22
申请人： UNIV NANTES , CENTRE NAT RECH SCIENT , PRUVOST JEREMY , LEGRAND JACK , FOUCAULT ALAIN
发明人： PRUVOST JEREMY , LEGRAND JACK , FOUCAULT ALAIN
IPC分类号： B01D11/04 , C12M1/00 , C12P1/00 , C12P23/00
CPC分类号： C12P1/00 , B01D11/0288 , C12M21/02 , C12M29/18 , C12M47/06 , C12P23/00 , C12R1/89
摘要： The invention relates to a method for the extraction of cellular compounds from micro-organisms of the type that comprises: an extraction step of said cellular compounds from said micro-organisms, characterised in that each step is characterised in a continuous manner, said extraction step being carried out separately from said culture step, and said extraction step being carried out in conditions of biocompatibility with said micro-organisms, followed by: at least one step for recovering said cellular compounds; at least one step for recirculating said micro-organisms towards said culture step.
摘要翻译：本发明涉及一种从该类微生物中提取细胞化合物的方法，该方法包括：从所述微生物中提取所述细胞化合物的提取步骤，其特征在于每个步骤以连续方式表征，所述提取步骤与所述培养步骤分开进行，所述提取步骤在与所述微生物的生物相容性的条件下进行，然后：至少一个步骤，用于回收所述细胞化合物; 用于将所述微生物再循环到所述培养步骤的至少一个步骤。

72. 发明申请

WO2007061943A8 PORTABLE PREPARATION, ANALYSIS, AND DETECTION APPARATUS FOR NUCLEIC ACID PROCESSING 审中-公开
标题翻译：便携式制备，分析和检测装置用于核酸加工
公开(公告)号：WO2007061943A8
公开(公告)日：2008-09-12
申请号：PCT/US2006044878
申请日：2006-11-20
申请人： APPLERA CORP , VANN CHARLES S , GREENSTEIN MICHAEL , CHIANG YUH-MIN
发明人： VANN CHARLES S , GREENSTEIN MICHAEL , CHIANG YUH-MIN
IPC分类号： C12M3/00 , G01N27/26
CPC分类号： G01N27/447 , C12M47/06 , C12Q1/6806 , C12Q1/686
摘要： The present teachings comprise a device and method for lysing and/or purifying biological sample. The device can comprise a cartridge having a chamber containing a biological sample receiving region, a plurality of electrodes, and one or more sieving matrices. The electrodes can be configured to lyse the biological sample through the production of a pulsed electrical field. The electrodes can also be configured to heat lyse the biological sample. The electrodes can also be configured to electrophoretically move the biological sample through one or more sieving matrices. A portion of the sample can be isolated on a membrane. The portion of the sample isolated on the membrane can be amplified and detected. A portion of the sample can be isolated in a collection area present in the cartridge. The portion of the sample isolated in the collection area can be removed from the cartridge.
摘要翻译：本教导包括用于裂解和/或纯化生物样品的装置和方法。该装置可以包括具有包含生物样品接收区域，多个电极和一个或多个筛分基质的腔室的药筒。电极可以被配置成通过产生脉冲电场来裂解生物样品。电极也可以被配置为热裂解生物样品。电极还可以被配置成通过一个或多个筛分基质电泳移动生物样品。样品的一部分可以在膜上分离。可以扩增和检测在膜上分离的样品的部分。样品的一部分可以在存在于盒中的收集区域中分离。分离在收集区域中的样品的部分可以从盒中取出。

73. 发明申请

WO2008075275A1 CELL LYSIS OR ELECTROPORATION DEVICE COMPRISING AT LEAST ONE PYROELECTRIC MATERIAL 审中-公开
标题翻译：包含至少一种PYROELECTRIC材料的细胞分析或电泳装置
公开(公告)号：WO2008075275A1
公开(公告)日：2008-06-26
申请号：PCT/IB2007/055116
申请日：2007-12-14
申请人： KONINKLIJKE PHILIPS ELECTRONICS N. V. , MUELLER, Manfred , JOHNSON, Mark, T. , GILLIES, Murray, F. , PONJEE, Marc, W., G.
发明人： MUELLER, Manfred , JOHNSON, Mark, T. , GILLIES, Murray, F. , PONJEE, Marc, W., G.
IPC分类号： A61N1/32
CPC分类号： A61N1/327 , A61N1/0416 , A61N1/042 , C12M35/02 , C12M47/06
摘要： The present invention relates to a cell lysis or electroporation device comprising at least one pyroelectric material.
摘要翻译：本发明涉及包含至少一种热电材料的细胞裂解或电穿孔装置。

74. 发明申请

WO2008067044A2 DISPERSION DEVICES FOR AGGREGATES 审中-公开
标题翻译：聚合物分散装置
公开(公告)号：WO2008067044A2
公开(公告)日：2008-06-05
申请号：PCT/US2007/080958
申请日：2007-10-10
申请人： MERIAL LIMITED , RIGAUT, Guillaume
发明人： RIGAUT, Guillaume
IPC分类号： C12M1/00 , C12N5/06
CPC分类号： C12M43/02 , C12M47/06 , C12N5/0603 , C12N2531/00
摘要： The present invention encompasses a flow-through dispersion device for the dispersion of cell aggregates, the device comprising a first or upstream inlet obstacle and a second or downstream outlet obstacle, each obstacle having at least one traversing hole wherein no two holes align. Between the two obstacles is a turbulence chamber, such that when a cell suspension is passed through the inlet holes, into the chamber and then exits the second obstacle, the turbulence within the chamber disrupts and disperses cell aggregates. The device optionally may have more than two obstacles and turbulence chambers and multiple units may be placed in series to increase the amount of turbulence and time applied to a volume of cells. The invention further encompasses methods of using the device to disperse cell aggregates, and methods of culturing cells that involve seeding cultures and maintaining dispersed individual cells.
摘要翻译：本发明包括用于细胞团聚体分散的流通式分散装置，该装置包括第一或上游进口障碍物和第二或下游出口障碍物，每个障碍物具有至少一个横向孔其中没有两个孔对齐。在两个障碍物之间是湍流室，使得当细胞悬液通过入口孔进入室并随后离开第二障碍物时，室内的湍流扰乱并分散细胞聚集体。该设备可选地可以具有多于两个的障碍物，并且湍流室和多个单元可以串联放置以增加施加到一定体积的细胞的湍流量和时间量。本发明还包括使用该装置分散细胞聚集体的方法，以及培养包括接种培养物和维持分散的单个细胞的细胞的方法。

75. 发明申请

WO2006105251A3 ELECTROSONIC CELL MANIPULATION DEVICE AND METHOD OF USE THEREOF 审中-公开
标题翻译：电子细胞操作装置及其使用方法
公开(公告)号：WO2006105251A3
公开(公告)日：2008-03-27
申请号：PCT/US2006011565
申请日：2006-03-29
申请人： GEORGIA TECH RES INST , FEDOROV ANDREI , DEGERTEKIN FAHRETTIN
发明人： FEDOROV ANDREI , DEGERTEKIN FAHRETTIN
IPC分类号： C12M3/00 , C12N13/00 , C12N15/87
CPC分类号： C12M35/02 , C12M35/04 , C12M47/04 , C12M47/06 , C12N13/00
摘要： In a method of injecting a substance into a living cell, the substance, the cell and a liquid are placed into a tapering passage (312). Energy is applied, thereby inducing poration. To sort cells, a cellular suspension is placed in a tapering passage (312), including a narrow end that defines an opening (314) that has a dimension corresponding to a cell size. An acoustic wave is applied by a transducer (330), thereby forcing cells having a cell size smaller than the selected cell size through the opening (312). To extract material from a cell, an electric field, formed between a first electrode (320) and a second electrode (322), and an acoustic wave are applied, thereby causing the cell membrane to allow the material to pass out of the cell.
摘要翻译：在将物质注入活细胞的方法中，物质，细胞和液体被放置在锥形通道（312）中。施加能量，从而诱导发芽。为了对细胞进行分类，将细胞悬浮液放置在锥形通道（312）中，包括限定具有对应于细胞尺寸的尺寸的开口（314）的窄端。声波由换能器（330）施加，从而通过开口（312）迫使具有小于所选单元尺寸的单元尺寸的单元。为了从电池提取材料，施加形成在第一电极（320）和第二电极（322）之间的电场和声波，从而使细胞膜允许材料通过电池。

76. 发明申请

WO2006060282A2 MIXING DEVICES FOR CHEMICAL LYSIS OF CELLS 审中-公开
标题翻译：化学物质的混合装置
公开(公告)号：WO2006060282A2
公开(公告)日：2006-06-08
申请号：PCT/US2005042797
申请日：2005-11-28
申请人： MERIAL LTD , DETRAZ NOEL JOSEPH FRANCOIS , RIGAUT GUILLAUME
发明人： DETRAZ NOEL JOSEPH FRANCOIS , RIGAUT GUILLAUME
IPC分类号： C12P1/00 , C12N1/06
CPC分类号： C12N1/06 , B01F5/0268 , B01F5/0451 , B01F5/0682 , B01F5/0688 , B01F2005/0034 , C12M47/06
摘要： The invention relates to flow-through devices for mixing fluids and uses thereof for mixing and for lysing cells to release biological compounds of interest. The invention particularly encompasses flow-through methods for mixing and chemical methods for the isolation and purification of plasmid DNA from cell culture.
摘要翻译：本发明涉及用于混合流体的流通装置及其用于混合和裂解细胞以释放感兴趣的生物化合物的用途。本发明特别包括用于混合的流通方法和用于从细胞培养物分离和纯化质粒DNA的化学方法。

77. 发明申请

WO2006042551A1 A BIOGAS PRODUCING FACILITY WITH ANAEROBIC HYDROLYSIS 审中-公开
标题翻译：一个生物技术生产设备与厌氧水解
公开(公告)号：WO2006042551A1
公开(公告)日：2006-04-27
申请号：PCT/DK2005/000677
申请日：2005-10-19
申请人： BIO-CIRCUIT APS , JENSEN, Jan , JENSEN, Preben
发明人： JENSEN, Jan , JENSEN, Preben
IPC分类号： C02F11/04
CPC分类号： C02F11/04 , C12M21/04 , C12M23/36 , C12M23/58 , C12M47/06 , Y02E50/343 , Y02W10/23
摘要： The present invention relates to a method and a facility for conversion of organic waste into biogas, i.e. a methane containing gas, with an improved efficiency and economy. The method comprises three consecutive steps of: i) digestion of the organic waste in a first reactor; ii) hydrolysis of the digested organic waste in an anaerobic hydrolysis tank; and iii) digestion of the hydrolyzed organic waste in a second reactor; wherein evolved gases are removed from the anaerobic hydrolysis tank. The biogas producing facility comprises a first reactor for holding organic waste for production of biogas by digestion and having an output for digested waste, and an anaerobic tank that is connected to the reactor output for anaerobic hydrolysis of the digested waste and having an output for hydrolysed material that is connected to an input of a second reactor for adding hydrolysed material to the content of the reactor and wherein a gas is passed through the headspace of the anaerobic hydrolysis tank for removal of gases from the digested waste. The anaerobic hydrolysis process and the evaporation and wash out of gases from the hydrolysis tank makes the energy content of material that has not been digested in the reactor easier and immediate available for bacterial digestion and the evaporation and wash out of gases in the hydrolysis tank further reduce inhibition of the bacteria and enhance the biogas production velocity and thus, the hydrolysed material is fed back into a reactor for further bacterial conversion into biogas. Furthermore, the gas that has been passed through the headspace of the anaerobic hydrolysis tank may be cooled in a heat exchanger so that the condensable gases of the gases removed from the anaerobic hydrolysis tank content condense, the condensed water containing the removed gases.
摘要翻译：本发明涉及将有机废物转化为沼气的方法和设备，即具有提高的效率和经济性的含甲烷气体。该方法包括以下三个步骤：i）在第一反应器中消化有机废物; ii）在厌氧水解槽中水解消化的有机废物; 和iii）在第二反应器中消化水解的有机废物; 其中放出的气体从厌氧水解槽中除去。沼气生产设施包括：第一反应器，用于通过消化保持用于生产沼气的有机废物，并具有用于消化废物的输出;以及厌氧池，其连接到反应器输出端，用于消化废物的厌氧水解并具有水解产物连接到第二反应器的输入的材料，用于将水解的材料加入到反应器的内容物中，并且其中气体通过厌氧水解槽的顶部空间，以从消化的废物中除去气体。厌氧水解过程以及水解槽中气体的蒸发和洗脱使得反应器中未被消化的材料的能量含量更容易，立即可用于细菌消化，并进一步蒸发和洗出水解罐中的气体减少细菌的抑制并提高生物气的生产速度，从而将水解的物质反馈回反应器进一步细菌转化成沼气。此外，已经通过厌氧水解槽的顶部空间的气体可以在热交换器中冷却，使得从厌氧水解槽内容物中除去的气体的可冷凝气体冷凝，含有除去的气体的冷凝水。

78. 发明申请

WO2006036757A2 HIGH TEMPERATURE FLOW-THROUGH DEVICE FOR RAPID SOLUBILIZATION AND ANALYSIS 审中-公开
标题翻译：用于快速溶解和分析的高温流动装置
公开(公告)号：WO2006036757A2
公开(公告)日：2006-04-06
申请号：PCT/US2005034045
申请日：2005-09-23
申请人： SANDIA NAT LAB , WEST JASON A A , HUKARI KYLE W , PATEL KAMLESH D , PETERSON KENNETH A
发明人： WEST JASON A A , HUKARI KYLE W , PATEL KAMLESH D , PETERSON KENNETH A
CPC分类号： B01L3/5027 , B01L7/00 , B01L2300/0877 , B01L2300/0887 , B01L2300/1827 , C12M47/06 , C12M47/20 , C12N1/066
摘要： Devices and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.
摘要翻译：提供了用于热裂解生物材料的装置和方法，例如营养细菌细菌和细菌孢子。描述了溶解细菌孢子的热溶解方法。公开了用于直接分析的系统，包括耦合到样品制备站的热裂解器。提供能够对生物材料进行样品裂解，标记和蛋白质指纹分析的集成系统，例如营养细菌细菌，细菌孢子和病毒。

79. 发明申请

WO2005028635A3 MICROFLUIDIC DIFFERENTIAL EXTRACTION CARTRIDGE 审中-公开
标题翻译：微流体微分萃取筒
公开(公告)号：WO2005028635A3
公开(公告)日：2005-06-16
申请号：PCT/US2004030899
申请日：2004-09-17
申请人： MICROFLUIDIC SYSTEMS INC
发明人： BELGRADER PHILLIP , YUAN BOB , AFLATOONI NIMA
IPC分类号： B01L3/00 , C12M1/42 , C12N20060101 , C12N1/06 , C12N13/00 , C12Q1/04
CPC分类号： B01L3/5027 , B01L3/5025 , B01L2200/0631 , B01L2200/0647 , B01L2200/10 , B01L2300/0636 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/1827 , B01L2400/0409 , B01L2400/0487 , C12M47/06 , C12N1/06 , C12N1/066 , C12N13/00
摘要： A differential extraction system is directed to a microfluid-based integrated cartridge to automate differential extraction of specific cell types within a mixed sample. The integrated cartridge includes a sonication module for Selective cell lysis, separating means to eliminate centrifugation, high surface area pillar chip modules to purify DNA from a cell lysate, and microfluid circuitry to integrate the steps in an automated platform.
摘要翻译：差分提取系统针对基于微流体的集成盒以自动化混合样品内特定细胞类型的差异提取。该集成盒包括用于选择性细胞裂解的超声处理模块，用于消除离心作用的分离装置，用于从细胞裂解物中纯化DNA的高表面积柱芯片模块以及用于将这些步骤集成到自动化平台中的微流体电路。

80. 发明申请

WO2004099384A2 SOLID PHASE CELL LYSIS AND CAPTURE PLATFORM 审中-公开
标题翻译：固体相细胞分析和捕获平台
公开(公告)号：WO2004099384A2
公开(公告)日：2004-11-18
申请号：PCT/US2004/013767
申请日：2004-05-03
申请人： SIGMA-ALDRICH CO. , KAPPEL, William, K. , MEHIGH, Richard, J. , JENKINS, Elizabeth, A.
发明人： KAPPEL, William, K. , MEHIGH, Richard, J. , JENKINS, Elizabeth, A.
IPC分类号： C12N
CPC分类号： C12N1/06 , C07K1/36 , C12M47/06 , C12N15/1003 , C12N15/1006 , C12N15/1017
摘要： The present invention provides containers, processes, and kits relating to the extraction or the extraction and isolation of a cellular component from a host cell. More specifically, the containers of the invention comprise a mouth; an interior surface comprising a sidewall formation and a bottom; a volume; a lytic reagent; and in some instances, a supported capture ligand. Methods and kits for the extraction or the extraction and isolation of a cellular component from a host cell using the containers described herein are also provided.
摘要翻译：本发明提供与从宿主细胞中提取或提取和分离细胞组分相关的容器，方法和试剂盒。更具体地，本发明的容器包括口; 包括侧壁结构和底部的内表面; 一卷溶解试剂; 并且在一些情况下，是支持的捕获配体。还提供了使用本文所述的容器从宿主细胞中提取或提取和分离细胞组分的方法和试剂盒。

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