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    • 56. 发明申请
    • METHOD, PHOTOBIOREACTOR AND PHOTOSYNTHESIS LAYERS FOR THE CULTURE OF PHOTOAUTOTROPHIC MICRO-ORGANISMS
    • 光自养培养微生物的方法,照片和光合生物反应器LAYERS
    • WO2013072495A3
    • 2013-08-22
    • PCT/EP2012072909
    • 2012-11-16
    • UNIV BERLIN HUMBOLDT
    • EHWALD RUDOLFBAEHR LARSWUESTENBERG ARNESOH JOEL HERVE
    • C12N1/12C12M1/00
    • C12M21/02C12M23/06C12M23/34C12M25/02C12M29/26C12M31/08C12N1/12
    • The invention relates to a method for cultivating photoautotrophic micro-organisms is proposed, comprising the following steps: provision of a photosynthesis layer (2), which contains a photosynthesis medium and photoautotrophic micro-organisms suspended therein, in a closed accommodation space having a gas-diffusion-inhibiting outer boundary (1), wherein the photosynthesis layer (2) has an average thickness of no more than 10 mm; exposure of the photosynthesis layer (2) without eddying or feeding through a gas in the photosynthesis medium while limiting the diffusive gas exchange of the accommodation space by the environment, wherein the supply of micro-organisms in the photosynthesis medium with hydrogen carbonate ions is maintained by storing hydrogen carbonate ions in the photosynthesis medium and/or providing a photosynthesis substrate in the form of carbon dioxide and hydrogen carbonate from a cell-free carbon storage medium (4) outside the photosynthesis layer; and conducting a gas formed during photosynthesis out of the accommodation space. The invention further relates to a photobioreactor for cultivating photoautotrophic micro-organisms, which comprises a closed accommodation space having a gas-diffusion-inhibiting outer boundary, wherein the accommodation space has a first compartment for forming a photosynthesis layer (2), which has an average thickness of no more than 10 mm, and at least one second compartment for accommodating a carbon storage medium (4), which is separated from the first compartment by at least one membrane that is permeable to at least one photosynthesis substrate selected from the group comprising carbon dioxide and hydrogen carbonate ions.
    • 它提出了一种方法,用于光合自养微生物的培养,其包括: - 提供一个光合作用介质并悬浮含光合作用层在其中光自养微生物(2)与气体扩散阻碍外边界(1)封闭的容纳腔,其中,所述光合作用层(2)具有的平均厚度 具有不超过10毫米; - 无需涡旋暴露光合作用层(2)或使气体在光合作用中,同时限制与环境的容纳空间,的扩散的气体交换,其中,在与碳酸氢根离子的光合介质中的微生物的通过存储碳酸氢根离子在光合作用介质和/或提供一个光合作用基板供给 在从不含细胞的碳存储介质的二氧化碳和/或氢的形式(4)被保持光合作用层的外侧; 和 - 从导出容纳空间光合作用过程中形成的气体。 此外,用于培养光合自养微生物的光生物反应器,提出了一种包括具有气体扩散抑制外边界的封闭容纳空间,其中所述接收空间: - 用于形成光合作用层的第一隔室(2)具有不大于10毫米的平均厚度 包括: - 以及至少一个用于含有从第一隔室通过至少一个膜,其是可渗透的从含有二氧化碳和碳酸氢根离子的组中选择的至少一种光合作用衬底分离碳存储介质(4)的第二隔室。